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Spo11 (NM_012046) Mouse Tagged ORF Clone
CAT＃: MR226168
- TrueORF®
Spo11 (Myc-DDK-tagged) - Mouse sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae) (Spo11), transcript variant 1
ORF Plasmid: tGFP
"NM_012046" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI449549; Spo11a; Spo11b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226168 representing NM_012046
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTTCGCGCCTATGGGGCCCGAGGCCTCGTTCTTCGACGCCCTGGATCGGCACAGGGCTTCCCTGT TGGCCATGGTGAAGAGAGGCGCAGGGGAGACCCCTGCCGGGGCCACCCGCGTGGCCTCTAGTTCTGAGGT TCTTACAGCTATAGAAAATATTATCCAAGACATAATCAAAAGCTTGGCAAGAAATGAAGTGCCTGCCTTC ACAATAGACAACAGATCGAGCTGGGAAAACATAATGTTTGATGATTCTGTCGGCCTTCGGATGATACCTC AGTGTACCACAAGAAAAATCAGAAGCGATTCACCAAAATCAGTTAAGAAATTCGCTCTGATTCTGAAAGT ATTGTCCATGATTTATAAATTAATACAGAGCGACACTTATGCAACCAAGAGAGACATATACTACACTGAC AGCCAGCTCTTTGGGAACCAGGCTGCGGTGGACAGCGCCATCGATGACATTTCCTGTATGCTGAAAGTGC CCAGGAGGAGTCTGCACGTGCTATCTACTTCCAAGGGATTGATTGCTGGCAACTTGAGATACATGGAGGA AGATGGTACCAGAGTCCAGTGTACCTGTAGTGCCACGGCTACTGCTGTGCCGACTAACATTCAAGGAATG CAGCATCTGATCACAGATGCGAAGTTTCTGTTAATAGTCGAGAAGGATGCAACATTTCAGCGGCTCCTGG ACGACAACTTCTGCAGCAGGATGTCCCCGTGCATCATGGTTACGGGAAAGGGCGTTCCAGATCTGAACAC GAGGCTCTTGGTAAAGAAGCTGTGGGACACCTTTCATATTCCTGTTTTCACACTGGTCGATGCAGATCCC TACGGCATCGAGATAATGTGCATCTATAAGTACGGATCCATGTCCATGTCTTTTGAAGCTCACAATCTCA CTATCCCAACAATCAGATGGCTTGGTCTCCTCCCTTCTGATATCCAGAGGTTAAATATACCTAAGGATAG TTTGATCCCACTGACAAAGCATGACCAGATGAAGCTGGACAGCATCCTGAAGAGGCCTTACATTACCTAC CAGCCCCTCTGGAAAAAAGAGCTGGAGATGATGGCAGACTCTAAGATGAAGGCAGAGATCCAAGCCTTGA CCTTGCTGTCGTCAGACTACCTGTCCAGAGTGTACTTACCCAACAAGCTGAGGTTTGGAGGATGGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226168 representing NM_012046
Red=Cloning site Green=Tags(s) MAFAPMGPEASFFDALDRHRASLLAMVKRGAGETPAGATRVASSSEVLTAIENIIQDIIKSLARNEVPAF TIDNRSSWENIMFDDSVGLRMIPQCTTRKIRSDSPKSVKKFALILKVLSMIYKLIQSDTYATKRDIYYTD SQLFGNQAAVDSAIDDISCMLKVPRRSLHVLSTSKGLIAGNLRYMEEDGTRVQCTCSATATAVPTNIQGM QHLITDAKFLLIVEKDATFQRLLDDNFCSRMSPCIMVTGKGVPDLNTRLLVKKLWDTFHIPVFTLVDADP YGIEIMCIYKYGSMSMSFEAHNLTIPTIRWLGLLPSDIQRLNIPKDSLIPLTKHDQMKLDSILKRPYITY QPLWKKELEMMADSKMKAEIQALTLLSSDYLSRVYLPNKLRFGGWI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_012046 |
| ORF Size | 1188 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_012046.2, NP_036176.1 |
| RefSeq Size | 1716 bp |
| RefSeq ORF | 1191 bp |
| Locus ID | 26972 |
| UniProt ID | Q9WTK8 |
| MW | 44.6 kDa |
| Gene Summary | Isoform 1: Component of a topoisomerase 6 complex specifically required for meiotic recombination. Together with TOP6BL, mediates DNA cleavage that forms the double-strand breaks (DSB) that initiate meiotic recombination (PubMed:26917764). The complex promotes relaxation of negative and positive supercoiled DNA and DNA decatenation through cleavage and ligation cycles. Essential for the phosphorylation of SMC3, HORMAD1 and HORMAD2 (PubMed:22346761).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209757 | Spo11 (untagged) - Mouse sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae) (Spo11), transcript variant 1, (10ug) |
CNY 5,488.00 |
|
| MG226168 | Spo11 (tGFP-tagged) - Mouse sporulation protein meiosis-specific SPO11 homolog (S. cerevisiae) (Spo11) transcript variant 1, (10ug) |
CNY 4,370.00 |
|
| MR226168L3 | Lenti ORF clone of Spo11 (Myc-DDK-tagged) - Mouse sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae) (Spo11), transcript variant 1 |
CNY 5,890.00 |
|
| MR226168L4 | Lenti ORF clone of Spo11 (mGFP-tagged) - Mouse sporulation protein, meiosis-specific, SPO11 homolog (S. cerevisiae) (Spo11), transcript variant 1 |
CNY 5,890.00 |
