Scn4b (NM_001013390) Mouse Tagged ORF Clone

CAT#: MR226154

  • TrueORF®

Scn4b (Myc-DDK-tagged) - Mouse sodium channel, type IV, beta (Scn4b)

ORF Plasmid: DDK tGFP



  "NM_001013390" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,081.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


SCN4B Rabbit polyclonal Antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

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Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms Gm1471
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR226154 representing NM_001013390
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTCCCGGGCTGGGAACCGAGGCAATACTCAGGCGAGATGGCTGGGCACTGGGCTTTTGGGTCTCTTCC
TGCTTCCCATGTACCTGTCGTTGGAGGTATCTGTGGGAAAGGCCACCACCATCTACGCTATTAACGGCTC
ATCGATCCTGCTGCCCTGCACCTTCTCCAGCTGTTATGGCTTTGAGAACCTCTACTTCAAGTGGTCCTAC
AATAACAGCGAAACATCCAGGATTCTCATCGACGGGATCGTGAAGAACGATAAGTCTGACCCTAAGGTGA
GAGTGAAGGATGATGATCGAATCACCCTAGAGGGCTCCACCAAGGAGAAGACGAATAACATCTCCATCCT
CTTGAGTGACCTGGAGTTCAGTGACACGGGCAGATACACCTGCTTCGTGAGGAACCCCAAGGAGAAGGAC
TTGAACAACTCTGCCACCATCTTCCTCCAAGTGGTTGATAAATTGGAAAAAGTGGACAACACGGTGACTC
TCATCATCCTGGCTGTGGTGGGCGGGGTCATTGGACTTCTTGTTTGCATCCTTCTGCTGAAGAAGCTCAT
CACCTTCATCCTGAAGAAGACCCGAGAGAAGAAGAAGGAGTGTCTCGTGAGTTCCTCTGGGAATGACAAC
ACAGAGAACGGGTTGCCTGGCTCCAAGGCAGAAGAGAAGCCACCCACAAAAGTG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR226154 representing NM_001013390
Red=Cloning site Green=Tags(s)

MSRAGNRGNTQARWLGTGLLGLFLLPMYLSLEVSVGKATTIYAINGSSILLPCTFSSCYGFENLYFKWSY
NNSETSRILIDGIVKNDKSDPKVRVKDDDRITLEGSTKEKTNNISILLSDLEFSDTGRYTCFVRNPKEKD
LNNSATIFLQVVDKLEKVDNTVTLIILAVVGGVIGLLVCILLLKKLITFILKKTREKKKECLVSSSGNDN
TENGLPGSKAEEKPPTKV

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001013390
ORF Size 684 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001013390.3
RefSeq Size 4244 bp
RefSeq ORF 687 bp
Locus ID 399548
UniProt ID Q7M729
MW 25.6 kDa
Gene Summary Modulates channel gating kinetics. Causes negative shifts in the voltage dependence of activation of certain alpha sodium channels, but does not affect the voltage dependence of inactivation. Modulates the susceptibility of the sodium channel to inhibition by toxic peptides from spider, scorpion, wasp and sea anemone venom (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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