United States
Germany
Japan
United Kingdom
China
Ufl1 (NM_026194) Mouse Tagged ORF Clone
CAT＃: MR225944
- TrueORF®
Ufl1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810074P20 gene (1810074P20Rik)
ORF Plasmid: tGFP
"NM_026194" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 5,816.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1810074P20Rik; AI429228; Kiaa0776; Maxer; mKIAA0776; Rcad |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225944 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGCCTGGGAGGAGATCCGGCGGCTGGCGGCTGACTTCCAGCGGGCGCAGTTTGCAGAGTCCA CGCAAAGGTTGTCAGAGCGGAACTGCATTGAGATTGTGAATAAATTGATCTCTCAGAAACAGCTGGAAGT AGTCCATACACTTGACGGCAAGGAATATATCACTCCAGCCCAAATCAGTAAAGAAATGAGAGACGAGCTA CATGTCCGAGGTGGTCGAGTAAACATTGTTGACTTACAACAGGTTATTAATGTGGACCTGACTCATATCG AGAGTAGAGTCAGTGATATTATTAAATCAGAGAAGCACGTGCAGATGGTACTGGGACAACTGATTGATGA GAACTATCTGGATCGGTTATCAGAAGAGGTGAATGATAAATTGCAAGAAAGTGGTCAAGTAACTGTCTCA GAACTGTGTAAAGCCTATGACCTTCCTGGGGATTTTCTGACACAGGCATTAACTCAGCGACTTGGCCGGA TTATCAATGGCCATCTGGACCTTGATAACAGAGGAGTGATTTTCACTGAGGCCTTTGTAGCTCGCCATAA AGCACGTATCCGCGGACTCTTCAGTGCCATTACACGGCCTACACCAGTGAACTCTTTGGTTTCAAAATAT GGATTTCAGGAACAGCTCCTTTACTCTGTGCTCGAGGACCTCGTCAGCACTGGCCGCTTACGAGGCACCG TGGTCGGCGGGCGACAGGATAAAGCAGTCTTTGTCCCTGACATCTACTCCAGGACACAGAGTACTTGGGT GGACTCCTTTTTCAGGCAGAATGGCTATCTAGAATTTGACGCTTTATCCAGACTTGGAATCCCAGATGCT GTGAACTATATAAAGAAAAGGTATAAGAATACCCAACTCTTGTTTTTGAAGGCAACTTGTGTTGGCCAAG GACTCGTAGACCAGGTGGAAGCATCCGTAGAGGAAGCCATCAGCTCAGGGACCTGGGTTGACATTTCGCC TCTGCTACCCAGTTCCTTGTCAGTTGAAGATGCTGCTATGCTGCTCCAGCAGGTGATGAGACCATTCGGC AAGCTCGCTTCAGCTATAGTCTTTAGTGACACGGTTGTGGTGAGTGAAAAGTTCATAACTGACTGCACAG GACTGTTCAGCGAACGGATGCACCAAAAAGCTGAGAAGGAAATGAAAAATAATCCTGTGCATTTAATCAC TGAAGAAGATCTGAAACAAATTTCCATTTTAGAAAGTGTTAATACAAGTAAAAAGGATAAAAAAGATGAA CGCAGGAAGAAAGCAACAGAGGGCTCTGGAAGTGTAAGAGGAGGAGGAGGGGGCAATGCCAGAGAATATA AAATTAAGAAAACCAAGAAGAAGGGAAGAAAAGATGAGGACAGTGATGATGAGTCCCAGTCGTCGCATGG TGGAAAGAAGAAGCCGGACATCACATTCATGTTCCAGGATGAAATTGAAGATTGCTTAAGGAAACACATT CAGGATGCCCCTGAGGAGTTTATTTCAGAGCTTGCAGAATACTTAATAAAGCCTCTTAATAAAATGTATC TTGAGGTAGTACGTTCAGTCTTCATGTCTTCAACATCTGCTTCTGGAACTGGCAGAAAACGCACAATCAA GGACTTACAAGAAGAGGTTTCAAACCTATACAATAATATCAGATTATTTGAAAAGGGAATGAAATATTTT GCAGATGACACACAGACTGCTCTTACCAAGCACTTGCTGAAGACAGTGTGTACCGATATCACTAACCTCA TGTTCAACTTCCTAGCCTCAGATTTCCTCATGGCAGTGGAGGAGCCTGCAGCCATTACAAGTGATATAAG AAAAAAAATTTTAAGCAAATTGACAGAAGAAACCAAAGTAGCCCTCACAAAACTCCATAACTCTCTCAAT GAAAAAAGCATAGAAGACTTCCTGTCTTGCCTGGATTCTGCCACAGAAGCGTGTGACATTATGGTGAAAA AGGGAGACAAGAAAAGGGAAAGACAAATCCTCTTCCAGCATCGACAGGCTCTGTGTGAGCAGCTGAAGGT CACAGAAGACCCTGCTCTGATTTTACACCTCACAGCAGTCCTGTTATTTCAGCTCTCAACCCACAGCATG CTCCACGCACCTGGAAGGTGTGTCCCACAGATCATCGCGTTTCTTCACAGCAAAATCCCAGAGGATCAAC ATACTCTCTTGGTAAAATATCAAGGTTTGGTTGTGAAACAGTTAGTCAGTCAAAATAAGAAGACTGGGCA GGGAGAGGATCCGTCAAGCGATGAATTAGACAAGGAACAACATGATGTCACAAACGCTACTCGGAAAGAG CTTCAGGAACTGTCATTATCCATTAAGGACCTTGTTCTCAAGTCCAGGAAATCATCTGTCACAGAGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225944 protein sequence
Red=Cloning site Green=Tags(s) MADAWEEIRRLAADFQRAQFAESTQRLSERNCIEIVNKLISQKQLEVVHTLDGKEYITPAQISKEMRDEL HVRGGRVNIVDLQQVINVDLTHIESRVSDIIKSEKHVQMVLGQLIDENYLDRLSEEVNDKLQESGQVTVS ELCKAYDLPGDFLTQALTQRLGRIINGHLDLDNRGVIFTEAFVARHKARIRGLFSAITRPTPVNSLVSKY GFQEQLLYSVLEDLVSTGRLRGTVVGGRQDKAVFVPDIYSRTQSTWVDSFFRQNGYLEFDALSRLGIPDA VNYIKKRYKNTQLLFLKATCVGQGLVDQVEASVEEAISSGTWVDISPLLPSSLSVEDAAMLLQQVMRPFG KLASAIVFSDTVVVSEKFITDCTGLFSERMHQKAEKEMKNNPVHLITEEDLKQISILESVNTSKKDKKDE RRKKATEGSGSVRGGGGGNAREYKIKKTKKKGRKDEDSDDESQSSHGGKKKPDITFMFQDEIEDCLRKHI QDAPEEFISELAEYLIKPLNKMYLEVVRSVFMSSTSASGTGRKRTIKDLQEEVSNLYNNIRLFEKGMKYF ADDTQTALTKHLLKTVCTDITNLMFNFLASDFLMAVEEPAAITSDIRKKILSKLTEETKVALTKLHNSLN EKSIEDFLSCLDSATEACDIMVKKGDKKRERQILFQHRQALCEQLKVTEDPALILHLTAVLLFQLSTHSM LHAPGRCVPQIIAFLHSKIPEDQHTLLVKYQGLVVKQLVSQNKKTGQGEDPSSDELDKEQHDVTNATRKE LQELSLSIKDLVLKSRKSSVTEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_026194 |
| ORF Size | 2382 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_026194.1, NM_026194.2, NM_026194.3, NM_026194.4, NP_080470.2 |
| RefSeq Size | 4335 bp |
| RefSeq ORF | 2382 bp |
| Locus ID | 67490 |
| UniProt ID | Q8CCJ3 |
| MW | 89.5 kDa |
| Gene Summary | E3 protein ligase that mediates ufmylation, the covalent attachment of the ubiquitin-like modifier UFM1 to substrate proteins, a post-translational modification on lysine residues of proteins that may play a crucial role in a number of cellular processes. Mediates DDRGK1 ufmylation and may regulate the proteasomal degradation of DDRGK1 and CDK5RAP3 thereby modulating NF-kappa-B signaling. May also play a role in nuclear receptor-mediated transcription through TRIP4 ufmylation. May play a role in the unfolded protein response, mediating the ufmylation of multiple proteins in response to endoplasmic reticulum stress. Anchors CDK5RAP3 in the cytoplasm, preventing its translocation to the nucleus which allows expression of the CCND1 cyclin and progression of cells through the G1/S transition.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221639 | Ufl1 (untagged) - Mouse RIKEN cDNA 1810074P20 gene (1810074P20Rik), (10ug) |
CNY 10,170.00 |
|
| MG225944 | Ufl1 (tGFP-tagged) - Mouse RIKEN cDNA 1810074P20 gene (1810074P20Rik), (10ug) |
CNY 6,370.00 |
|
| MR225944L3 | Lenti ORF clone of Ufl1 (Myc-DDK-tagged) - Mouse RIKEN cDNA 1810074P20 gene (1810074P20Rik) |
CNY 7,700.00 |
|
| MR225944L4 | Lenti ORF clone of Ufl1 (mGFP-tagged) - Mouse RIKEN cDNA 1810074P20 gene (1810074P20Rik) |
CNY 7,700.00 |
