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Gli2 (NM_001081125) Mouse Tagged ORF Clone
CAT＃: MR225917
- TrueORF®
Gli2 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2)
ORF Plasmid: tGFP
"NM_001081125" in other vectors (4)
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CNY 10,536.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW546128 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225917 representing NM_001081125
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACTTCTGCCCCAGCCCCTGCACTGGAGAAGAAAGAAGCCAAGAGTGGTCTCTTGGAGGACAGCA GCTTCCCCGACCCAGGGAAAAAGGCCTGTCCTCTGGCGGTGGCCGCAGCTGTAGCCGCCCACGGAGTTCC TCAGCAGCTCCTGCCGGCTTTCCACGCGCCTTTGCCGATTGACATGAGACACCAGGAGGGAAGGTACCAT TATGACCCTCACTCTGTCCACAGTGTACACGGGCCTCCCACCCTAAGTGGCAGCCCCTTCATCTCAGATA TCTCCTTGATACGACTTTCTCCACACCCTGCTGGCCCTGGAGAGTCACCCTTCAGCGCCCACCACCCCTA CGTGAACCCCCATATGGAGCACTACCTCCGGTCTGTGCACAGCAGCCCCACACTCTCAATGATCTCTGCC GCCAGGGGCCTCAGCCCTGCTGATGTGGCCCACGAACATCTGAAAGAGAGGGGACTCTTTAGCCTCGCAG CCCCAGGCACCAACCCTTCAGACTATTACCACCAGATGACCCTCATGGCAAGCCACCCCACCCCTTATGG GGACCTTCTAATGCAGAGCGGGGGTGCTGCTAGCGCACCCCATCTCCATGACTACCTCAACCCTGTGGAT GCATCACGATTCTCTAGTCCACGTGTGACCCCACGACTGAGCCGCAAGCGGGCTCTGTCCATCTCCCCGC TCTCAGATGCCAGCCTCGACCTACAACGCATGATTCGGACCTCTCCCAACTCGCTGGTAGCTTACATCAA CAACTCCAGGAGCAGCTCAGCAGCCAGTGGCTCTTATGGACATCTGTCTGCTGGTGCCCTCAGCCCAGCC TTCACTTTTCCCCACCCCATCAATCCTGTGGCCTACCAGCAGATCCTGAGCCAGCAGCGGGGCCTGGGCT CAGCCTTTGGACACACACCACCCCTGATCCAGCCTTCACCCACCTTCTTGGCCCAGCAGCCCATGACTCT CACCTCCATCAGCACCATGCCTACCCAACTCAGCAGCAGTAGCAGCAACTGTCTAAATGATGCCAACCAG AACAAGCAGAACAGCGAGTCAGCTGTGAGCAGCACCGTGAACCCCATCACCATTCATAAGCGGAGCAAGG TCAAGACTGAGGCTGAGGGCCTGCGTCCAGCATCCCCGCTTGGACTGACACAGGAGCAGCTGGCCGATCT CAAGGAAGACCTGGACAGGGATGACTGTAAGCAGGAGGCCGAGGGTGTCATCTACGAGACCAACTGCCAC TGGGCAGACTGCACCAAGGAGTATGACACACAGGAGCAGCTGGTGCATCATATCAACAATGAACACATCC ACGGGGAGAAGAAGGAGTTCGTGTGCCGCTGGCAGGCCTGCACGAGAGAGCAGAAGCCCTTCAAGGCCCA GTACATGCTGGTTGTTCACATGCGCAGACACACGGGTGAGAAGCCACACAAGTGCACGTTCGAAGGCTGT TCCAAGGCCTACTCTCGCCTGGAGAACCTGAAGACACACCTGCGTTCACACACAGGAGAGAAGCCATATG TGTGTGAACACGAAGGCTGTAACAAAGCCTTCTCCAATGCCTCAGACCGCGCCAAGCACCAGAACCGCAC TCACTCCAATGAGAAACCCTACATCTGCAAGATCCCAGGCTGCACCAAGAGGTACACAGACCCCAGCTCA CTCCGCAAGCATGTGAAGACTGTCCATGGGCCAGACGCCCATGTCACCAAGAAACAGCGTAATGATGTGC ATGTCCGTGCTCCACTGCTCAAGGAGAATGGGGATAATGAGGCCAGCGCCGAGCCAGGTGGCCGGGGACC TGAGGAGAGTGTGGAGGCCAGTAGCACCAGCCACACTGTGGAGGACTGCCTACATATCAAAGCCATCAAG ACAGAGAGCTCCGGGCTTTGTCAGTCCAGCCCCGGGGCCCAGTCATCCTGCAGCAGCGAGCCCTCTCCCC TGGGCAGTGCCCCCAACAATGACAGTGGCATGGAGATGCCGGGGACAGGGCCTGGGAGTCTGGGAGACCT GACAGCACTGGCTGACACGTGTCCAGGAGCTGACACCTCAGCCCTGGCTGCACCCTCCACTGGTGGCCTG CAGCTGCGCAAACACATGAGCACCGTGCATCGCTTTGAGCAGCTGAAGAGAGAGAAGCTCAAGTCACTGA AGGATTCCTGCTCGTGGGCCGGCCCAGCTCCACACACCCGCAACACCAAGCTGCCTCCCCTTCCAGTCAA TGGTTCTGTCCTGGAAAACTTCAACAATACAGGGGGCGGTGGACCGGCAGGACTGCTGCCCAGCCAGCGG CTACCAGAGCTGACCGAAGTGACGATGCTGAGCCAGCTGCAGGAACGAAGAGACAGCTCCACCAGCACCA TGAGCTCGGCCTACACTGTGAGCCGCCGCTCCTCTGGCATCTCCCCATACTTCTCTAGCCGTCGCTCCAG CGAGGCTTCGCCTCTCGGTGGCCTACGCCCGCACAACGCCAGCTCAGCAGACTCCTATGACCCCATCTCC ACAGATGCCTCTCGGCGCTCCAGTGAAGCCAGCCAGTGCAGTGGCGGTGGCCCAGGGCTGCTCAACCTCA CACCTGCGCAGCAGTACAACCTGCGTGCCAAGTACGCAGCGGCCACAGGTGGACCACCGCCCACGCCACT GCCGGGCCTCGATCGTGTAAGCCTTCGTACCCGCCTGGCCTTGCTGGATGCTCCTGAGCGTGCACTTCCT GGTGCCTGCCCACATCCACTGGGGCCACGGCGTGGCAGCGATGGGCCTACCTATAGCCATGGTCATGGCC ATGGCTACGCAGGTGCGGCTCCAGCATTCCCCCACGAGGGGCCAAACAGCAGCACACGGCGGGCCAGCGA CCCTGTGCGGCGCCCTGACCCCCTTATTCTGCCTCGAGTGCAACGTTTCCACAGTACCCACAACATGAAT CCAGGTTCACTGCCACCCTGCGCTGATCGGCGTGGCCTGCACGTACAGAGCCACCCCAGCGTAGACAGCA ACCTGACCCGCAACGCCTACTCTCCCAGACCCCCTAGCATCAATGAGAACGTGGTGATGGAGGCCGTGGC TGCTGGGGTAGACGGCCCAGGGCTAGAGTGCGACCTGGGGCTGGTGGAGGATGAGCTGGTGCTGCCAGAT GATGTGGTACAGTACATCAAGGCTCACACCGGTGGTACCTTGGATGACGGCATTCGGCAGGGGTATCCCA CAGAAGGTACTGGCTTCCCCGAGAACTCTAAGCTGCCCAGTCCTGGGCTACAAGGCCACCGCAGGCTAGC AGCTGCCGACTCCAACATGGGTCCTTCTGCTCCTGGACTCGGGGGCTGCCAGCTGAGCTACAGCCCCTCC TCCAACCTCAACAAGAGCAACATGCCTGTGCAGTGGAATGAGGTGAGTTCTGGCACCGTGGATGCCCTGC CTACCCAGGTGAAGCCACCTCCTTTCCCTCACAGCAACCTGGCTGTGGTCCAACAGAAGCCAGCCTTTGG CCAGTATCCAGGATATAATCCACAATCCGTGCAGAGCAGCTCCGGAGGTCTAGACAGCACCCAGCCGCAC CTACAGCTTCGAGGAGCCCCCTCTGCATCAAGAGGGAGCTACACGCAACAGCCTCGACAGCCAGCTGCAG GCAGTCAGTGCCTGGGTATGAGTGCGGCCATGAGCCCGCAGGCCAGCTACAGCCAAGCCCACCCCCAGCT GAGCCCAAACATTGTCAGCGGATCTCTGAACCAGTTTTCTCCCTCCTGCAGCAATATGGCAGCCAAGCCC AGCCACCTGGGACTCCCTCAGCAAATGGAAGTTGTCCCCAATGCCACCATCATGAATGGCCATCAACGGG AGCACGGGGTCCCCAATTCATCCCTGGCTGCGGTGTCACAACCTCACCCAGTCCTGAGCTATCCCCAGCA GGACAGCTACCAACAGGGCTCCAACCTTCTGTCATCCCATCAGCCTGGCTTCATGGAGTCCCAGCAGAAC GCGGGCTTTGGTCTCATGCAGCCTCGGCCACCCCTGGAACCCAACACGGCCAGCCGTCACCGTGGAGTAC GTTCTGGGCAACAGCAGTTGTATGCCAGGACCACTGGCCAAGCCATGGTCACATCAGCCAACCAAGAGAC AGCAGAAGCTATGCCCAAGGGACCAGCAGGGACCATGGTATCCCTAGCTCCTCAGCCATCTCAGGACACA GGGCGGGCACAAGATCAGAACACGCTATACTACTATGGCCAGATCCACATGTATGAACAGAATGGAGGCT GCCCAGCCGTGCAGCCCCAGCCGCCACAACCACAAGCTTGCTCAGACAGTATCCAGCCTGAGCCTTTGCC TTCACCGGGAGTCAACCAGGTGTCTAGCACCGTGGACTCCCAGCTCCTGGAGCCCCCCCAGATTGACTTT GATGCCATCATGGATGATGGTGATCACTCGAGTTTGTTTTCTGGTGCACTGAGCCCAACCCTTCTCCACA ATCTCTCCCAGAATTCCTCACGCCTCACCACACCCCGGAATTCCTTGACACTGCCCTCCATCCCTGCGGG CATCAGCAACATGGCCGTGGGCGACATGAGTTCCATGCTCACCAGCCTGGCTGAAGAGAGCAAGTTTTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225917 representing NM_001081125
Red=Cloning site Green=Tags(s) METSAPAPALEKKEAKSGLLEDSSFPDPGKKACPLAVAAAVAAHGVPQQLLPAFHAPLPIDMRHQEGRYH YDPHSVHSVHGPPTLSGSPFISDISLIRLSPHPAGPGESPFSAHHPYVNPHMEHYLRSVHSSPTLSMISA ARGLSPADVAHEHLKERGLFSLAAPGTNPSDYYHQMTLMASHPTPYGDLLMQSGGAASAPHLHDYLNPVD ASRFSSPRVTPRLSRKRALSISPLSDASLDLQRMIRTSPNSLVAYINNSRSSSAASGSYGHLSAGALSPA FTFPHPINPVAYQQILSQQRGLGSAFGHTPPLIQPSPTFLAQQPMTLTSISTMPTQLSSSSSNCLNDANQ NKQNSESAVSSTVNPITIHKRSKVKTEAEGLRPASPLGLTQEQLADLKEDLDRDDCKQEAEGVIYETNCH WADCTKEYDTQEQLVHHINNEHIHGEKKEFVCRWQACTREQKPFKAQYMLVVHMRRHTGEKPHKCTFEGC SKAYSRLENLKTHLRSHTGEKPYVCEHEGCNKAFSNASDRAKHQNRTHSNEKPYICKIPGCTKRYTDPSS LRKHVKTVHGPDAHVTKKQRNDVHVRAPLLKENGDNEASAEPGGRGPEESVEASSTSHTVEDCLHIKAIK TESSGLCQSSPGAQSSCSSEPSPLGSAPNNDSGMEMPGTGPGSLGDLTALADTCPGADTSALAAPSTGGL QLRKHMSTVHRFEQLKREKLKSLKDSCSWAGPAPHTRNTKLPPLPVNGSVLENFNNTGGGGPAGLLPSQR LPELTEVTMLSQLQERRDSSTSTMSSAYTVSRRSSGISPYFSSRRSSEASPLGGLRPHNASSADSYDPIS TDASRRSSEASQCSGGGPGLLNLTPAQQYNLRAKYAAATGGPPPTPLPGLDRVSLRTRLALLDAPERALP GACPHPLGPRRGSDGPTYSHGHGHGYAGAAPAFPHEGPNSSTRRASDPVRRPDPLILPRVQRFHSTHNMN PGSLPPCADRRGLHVQSHPSVDSNLTRNAYSPRPPSINENVVMEAVAAGVDGPGLECDLGLVEDELVLPD DVVQYIKAHTGGTLDDGIRQGYPTEGTGFPENSKLPSPGLQGHRRLAAADSNMGPSAPGLGGCQLSYSPS SNLNKSNMPVQWNEVSSGTVDALPTQVKPPPFPHSNLAVVQQKPAFGQYPGYNPQSVQSSSGGLDSTQPH LQLRGAPSASRGSYTQQPRQPAAGSQCLGMSAAMSPQASYSQAHPQLSPNIVSGSLNQFSPSCSNMAAKP SHLGLPQQMEVVPNATIMNGHQREHGVPNSSLAAVSQPHPVLSYPQQDSYQQGSNLLSSHQPGFMESQQN AGFGLMQPRPPLEPNTASRHRGVRSGQQQLYARTTGQAMVTSANQETAEAMPKGPAGTMVSLAPQPSQDT GRAQDQNTLYYYGQIHMYEQNGGCPAVQPQPPQPQACSDSIQPEPLPSPGVNQVSSTVDSQLLEPPQIDF DAIMDDGDHSSLFSGALSPTLLHNLSQNSSRLTTPRNSLTLPSIPAGISNMAVGDMSSMLTSLAEESKFF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081125 |
| ORF Size | 4620 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 6708 bp |
| RefSeq ORF | 4635 bp |
| Locus ID | 14633 |
| UniProt ID | Q0VGT2 |
| MW | 165.5 kDa |
| Gene Summary | Functions as transcription regulator in the hedgehog (Hh) pathway (PubMed:9006072). Functions as transcriptional activator (PubMed:10806483). May also function as transcriptional repressor (PubMed:10433919). Requires STK36 for full transcriptional activator activity (PubMed:10806483). Binds to the DNA sequence 5'-GAACCACCCA-3' which is part of the TRE-2S regulatory element (By similarity). Is involved in the smoothened (SHH) signaling pathway (PubMed:10433919). Required for normal skeleton development (PubMed:9006072).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224662 | Gli2 (untagged) - Mouse GLI-Kruppel family member GLI2 (Gli2), (10ug) |
CNY 19,860.00 |
|
| MG225917 | Gli2 (tGFP-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2), (10ug) |
CNY 14,920.00 |
|
| MR225917L3 | Lenti ORF clone of Gli2 (Myc-DDK-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2) |
CNY 15,490.00 |
|
| MR225917L4 | Lenti ORF clone of Gli2 (mGFP-tagged) - Mouse GLI-Kruppel family member GLI2 (Gli2) |
CNY 15,490.00 |
