Mc4r (NM_016977) Mouse Tagged ORF Clone
CAT#: MR225777
- TrueORF®
Mc4r (Myc-DDK-tagged) - Mouse melanocortin 4 receptor (Mc4r)
ORF Plasmid: tGFP
"NM_016977" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | Fatb; Mc4-r; Pk; Pkcp |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225777 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACTCCACCCACCACCATGGCATGTATACTTCCCTCCACCTCTGGAACCGCAGCAGCTACGGGCTGC ACGGCAATGCCAGCGAGTCGCTGGGGAAGGGCCACCCGGACGGAGGATGCTATGAGCAACTTTTTGTTTC CCCCGAGGTGTTTGTGACTCTGGGTGTCATAAGCCTGTTGGAGAACATTCTAGTGATCGTGGCGATAGCC AAGAACAAGAACCTGCACTCACCCATGTACTTTTTCATCTGTAGCCTGGCTGTGGCAGATATGCTGGTGA GCGTTTCGAATGGGTCGGAAACCATCGTCATTACCCTGTTAAACAGTACGGATACGGATGCCCAGAGCTT CACCGTGAACATTGATAATGTCATTGACTCTGTGATCTGTAGCTCCTTGCTCGCATCCATTTGCAGCCTG CTTTCCATTGCGGTGGACAGGTATTTCACTATCTTTTACGCGCTCCAGTACCATAACATCATGACGGTTA GGCGGGTCGGGATCATCATAAGTTGTATCTGGGCAGCTTGCACTGTGTCAGGCGTCCTCTTCATCATTTA CTCGGACAGCAGCGCTGTCATCATCTGCCTCATTTCCATGTTCTTCACTATGCTAGTTCTCATGGCCTCT CTCTATGTCCACATGTTCCTGATGGCGAGGCTTCACATTAAGAGGATTGCTGTCCTCCCAGGCACAGGGA CCATCCGCCAGGGTACCAACATGAAGGGGGCGATTACCTTGACCATCCTGATTGGAGTCTTTGTTGTCTG CTGGGCCCCGTTCTTTCTCCATTTACTGTTCTACATCTCTTGCCCTCAGAATCCATACTGCGTGTGCTTC ATGTCTCATTTTAATTTGTATCTCATACTGATCATGTGTAACGCCGTCATCGACCCTCTCATTTATGCCC TCCGGAGTCAAGAACTGAGGAAAACTTTCAAAGAGATCATCTGTTTCTATCCTCTGGGAGGCATCTGTGA GTTGTCTAGCAGGTAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225777 protein sequence
Red=Cloning site Green=Tags(s) MNSTHHHGMYTSLHLWNRSSYGLHGNASESLGKGHPDGGCYEQLFVSPEVFVTLGVISLLENILVIVAIA KNKNLHSPMYFFICSLAVADMLVSVSNGSETIVITLLNSTDTDAQSFTVNIDNVIDSVICSSLLASICSL LSIAVDRYFTIFYALQYHNIMTVRRVGIIISCIWAACTVSGVLFIIYSDSSAVIICLISMFFTMLVLMAS LYVHMFLMARLHIKRIAVLPGTGTIRQGTNMKGAITLTILIGVFVVCWAPFFLHLLFYISCPQNPYCVCF MSHFNLYLILIMCNAVIDPLIYALRSQELRKTFKEIICFYPLGGICELSSRY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016977 |
ORF Size | 999 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016977.4 |
RefSeq Size | 2783 bp |
RefSeq ORF | 999 bp |
Locus ID | 17202 |
UniProt ID | P56450 |
MW | 37 kDa |
Gene Summary | This gene encodes a member of the melanocortin receptor family. Melanocortin receptors are transmembrane G-protein coupled receptors, which respond to small peptide hormones and exhibit diverse functions and tissue type localization. As part of the central nervous melanocortin system, the encoded protein is competitively bound by either melanocyte stimulating hormone or agouti-related protein to regulate energy homeostasis. Disruption of this gene promotes hyperphagia and obesity, and is associated with increased cholesterol levels and insulin resistance. [provided by RefSeq, Dec 2012] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208946 | Mc4r (untagged) - Mouse melanocortin 4 receptor (Mc4r), (10ug) |
CNY 2,400.00 |
|
MG225777 | Mc4r (tGFP-tagged) - Mouse melanocortin 4 receptor (Mc4r), (10ug) |
CNY 4,000.00 |
|
MR225777L3 | Lenti ORF clone of Mc4r (Myc-DDK-tagged) - Mouse melanocortin 4 receptor (Mc4r) |
CNY 4,750.00 |
|
MR225777L4 | Lenti ORF clone of Mc4r (mGFP-tagged) - Mouse melanocortin 4 receptor (Mc4r) |
CNY 4,750.00 |