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Gna12 (NM_010302) Mouse Tagged ORF Clone
CAT＃: MR225752
- TrueORF®
Gna12 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha 12 (Gna12)
ORF Plasmid: tGFP
"NM_010302" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI414047; AI504261; Galpha12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225752 representing NM_010302
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCCGGGGTGGTGCGGACCCTTAGCCGCTGCTTGCTGCCGGCCGAGGCCGGAGCCCGCGAGCGCAGGG CGGGCGCGGCGCGCGACGCGGAGCGCGAGGCCCGACGGCGCAGCCGCGACATCGACGCGCTGCTGGCCCG CGAGCGGCGCGCGGTGCGGCGGCTGGTCAAGATCCTGCTGCTGGGCGCCGGCGAGAGCGGCAAGTCCACC TTCCTCAAGCAGATGCGCATCATCCACGGCCGGGAGTTCGACCAGAAGGCGCTGCTGGAGTTCCGCGACA CCATCTTCGACAACATCCTTAAGGGTTCGAGGGTTCTTGTGGACGCTCGAGACAAGCTCGGCATTCCCTG GCAGCACTCTGAGAACGAGAAGCACGGGATGTTTCTGATGGCCTTCGAGAACAAGGCAGGGCTGCCTGTG GAGCCTGCCACCTTCCAGCTCTACGTGCCAGCCCTGAGTGCCCTCTGGAGAGACTCGGGGATCAGGGAAG CCTTCAGCCGCAGAAGCGAGTTCCAGCTGGGTGAATCAGTGAAGTACTTCCTGGATAACTTGGACCGGAT TGGCCAGCTGAACTACTTCCCCAGTAAGCAAGACATCCTGCTGGCTAGAAAGGCCACCAAGGGAATCGTG GAACATGACTTCGTTATAAAGAAAATCCCATTTAAGATGGTGGATGTGGGCGGCCAGAGGTCACAGCGCC AGAAGTGGTTCCAGTGCTTCGACGGCATCACATCTATCCTGTTCATGGTGTCCTCGAGCGAGTATGACCA GGTCCTCATGGAGGACAGGCGCACCAACCGGCTGGTGGAGTCCATGAACATCTTCGAGACCATCGTCAAC AACAAGCTCTTCTTCAACGTCTCCATCATCCTCTTCCTCAACAAGATGGACCTCCTGGTGGAGAAGGTGA AGTCTGTGAGCATTAAGAAGCACTTCCCAGATTTCAAGGGCGACCCGCACCGGCTGGAGGACGTCCAGCG CTACCTGGTGCAGTGCTTCGACAGGAAGCGCAGGAACCGCAGCAAGCCCCTGTTCCACCACTTCACCACC GCCATAGACACCGAGAACATCCGCTTCGTGTTTCATGCTGTGAAGGACACGATCCTGCAGGAGAACCTGA AAGACATCATGCTGCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225752 representing NM_010302
Red=Cloning site Green=Tags(s) MSGVVRTLSRCLLPAEAGARERRAGAARDAEREARRRSRDIDALLARERRAVRRLVKILLLGAGESGKST FLKQMRIIHGREFDQKALLEFRDTIFDNILKGSRVLVDARDKLGIPWQHSENEKHGMFLMAFENKAGLPV EPATFQLYVPALSALWRDSGIREAFSRRSEFQLGESVKYFLDNLDRIGQLNYFPSKQDILLARKATKGIV EHDFVIKKIPFKMVDVGGQRSQRQKWFQCFDGITSILFMVSSSEYDQVLMEDRRTNRLVESMNIFETIVN NKLFFNVSIILFLNKMDLLVEKVKSVSIKKHFPDFKGDPHRLEDVQRYLVQCFDRKRRNRSKPLFHHFTT AIDTENIRFVFHAVKDTILQENLKDIMLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010302 |
| ORF Size | 1137 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010302.2, NP_034432.1 |
| RefSeq Size | 1880 bp |
| RefSeq ORF | 1140 bp |
| Locus ID | 14673 |
| UniProt ID | P27600 |
| MW | 44.5 kDa |
| Gene Summary | Guanine nucleotide-binding proteins (G proteins) are involved as modulators or transducers in various transmembrane signaling systems (PubMed:19151758, PubMed:21212405, PubMed:22609986). Activates effector molecule RhoA by binding and activating RhoGEFs (ARHGEF12/LARG) (By similarity). GNA12-dependent Rho signaling subsequently regulates transcription factor AP-1 (activating protein-1) (PubMed:19151758, PubMed:21212405). GNA12-dependent Rho signaling also regulates protein phosphatese 2A activation causing dephosphorylation of its target proteins (By similarity). Promotes tumor cell invasion and metastasis by activating RhoA/ROCK signaling pathway and up-regulating proinflammatory cytokine production (By similarity). Inhibits CDH1-mediated cell adhesion in process independent from Rho activation (By similarity). Together with NAPA promotes CDH5 localization to plasma membrane (By similarity). May play a role in the control of cell migration through the TOR signaling cascade (PubMed:22609986).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208562 | Gna12 (untagged) - Mouse guanine nucleotide binding protein, alpha 12 (Gna12), (10ug) |
CNY 3,990.00 |
|
| MG225752 | Gna12 (tGFP-tagged) - Mouse guanine nucleotide binding protein alpha 12 (Gna12), (10ug) |
CNY 3,140.00 |
|
| MR225752L3 | Lenti ORF clone of Gna12 (Myc-DDK-tagged) - Mouse guanine nucleotide binding protein, alpha 12 (Gna12) |
CNY 4,750.00 |
|
| MR225752L4 | Lenti ORF clone of Gna12 (mGFP-tagged) - Mouse guanine nucleotide binding protein, alpha 12 (Gna12) |
CNY 4,750.00 |
