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B4galt5 (NM_019835) Mouse Tagged ORF Clone
CAT＃: MR225689
- TrueORF®
B4galt5 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5)
ORF Plasmid: tGFP
"NM_019835" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 9430078I07Rik; AW049941; AW539721 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225689 representing NM_019835
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCGCCCGCCGGGGGCTGCTGCGGCTGCCGCGCCGCTCGCTGCTCGCCGCGCTCTTCTTCTTCTCGC TGTCGTCCTCGCTGCTCTACTTCGTCTATGTGGCGCCCGGCATAGTGAACACCTACCTCTTCATGGTGCA GGCTCAGGGCATCCTACTGCGGGACAATGTGAGGACCATCGGTGCCCAGGTGTACGAGCAGGTGGTCCGC AGCGCCTATGCCAAGAGGAACAGCAGCCTGAATGACTCAGATTATCCTCTGGACTTGAACCACAGTGAAG CCTTTCCCCCAACCACGACCTTTCTTCCTGAAGATTTCACCTACTTTGCCAACCACCCTTGCCCAGAGAG GCTCCCTTCCATGAAGGGCCCGATAGATATAAACATGAGTGAGATCGCAATGGACGATATTCATGAGCTG TTCTCCAGAGACCCGGCCATTAAGCTCGGAGGGCACTGGAAGCCTGCAGACTGTGTGCCTCGGTGGAAGG TGGCTATCCTCATCCCCTTCCGGAACCGCCATGAGCACCTCCCGGTCCTCCTGAGACACCTGCTCCCCAT GCTTCAGCGCCAGCGCCTGCAGTTCGCCTTCTATGTGATCGAGCAAGTTGGCACCCAGCCCTTTAACCGA GCCATGCTTTTCAATGTTGGTTTTCAAGAAGCCATGAAGGACTTGGATTGGGATTGTCTGATCTTCCATG ACGTCGATCACATACCTGAGAGTGACCGAAACTACTATGGCTGCGGGCAGATGCCCAGGCACTTTGCAAC CAAGCTGGACAAGTACATGTACCTGCTGCCCTACACTGAGTTTTTTGGTGGCGTGAGCGGCCTGACTGTA GAACAGTTTCGGAAAATCAATGGCTTTCCTAATGCCTTCTGGGGCTGGGGCGGAGAAGATGACGACTTGT GGAACAGGGTACAAAATGCAGGCTATTCTGTGAGCCGGCCAGAAGGGGACACAGGAAAATACAAGTCCAT TCCTCACCACCATCGAGGAGAGGTCCAGTTTCTTGGCAGGTACGCTCTGCTGAGGAAGTCAAAGGAGCGG CAAGGACTGGATGGCCTCAACAACCTGAACTACTCTGCGAATGTCACGTACGACGCCTTGTATAAAAACA TAACTGTCAACTTGACACCCGAGCTGGCTCAGGTGACTGAGTAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225689 representing NM_019835
Red=Cloning site Green=Tags(s) MRARRGLLRLPRRSLLAALFFFSLSSSLLYFVYVAPGIVNTYLFMVQAQGILLRDNVRTIGAQVYEQVVR SAYAKRNSSLNDSDYPLDLNHSEAFPPTTTFLPEDFTYFANHPCPERLPSMKGPIDINMSEIAMDDIHEL FSRDPAIKLGGHWKPADCVPRWKVAILIPFRNRHEHLPVLLRHLLPMLQRQRLQFAFYVIEQVGTQPFNR AMLFNVGFQEAMKDLDWDCLIFHDVDHIPESDRNYYGCGQMPRHFATKLDKYMYLLPYTEFFGGVSGLTV EQFRKINGFPNAFWGWGGEDDDLWNRVQNAGYSVSRPEGDTGKYKSIPHHHRGEVQFLGRYALLRKSKER QGLDGLNNLNYSANVTYDALYKNITVNLTPELAQVTEY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019835 |
| ORF Size | 1164 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019835.2, NP_062809.2 |
| RefSeq Size | 4205 bp |
| RefSeq ORF | 1167 bp |
| Locus ID | 56336 |
| UniProt ID | Q9JMK0 |
| MW | 45.3 kDa |
| Gene Summary | Catalyzes the synthesis of lactosylceramide (LacCer) via the transfer of galactose from UDP-galactose to glucosylceramide (GlcCer) (PubMed:21057870, PubMed:23882130, PubMed:30114188). LacCer is the starting point in the biosynthesis of all gangliosides (membrane-bound glycosphingolipids) which play pivotal roles in the CNS including neuronal maturation and axonal and myelin formation (PubMed:30114188). Plays a role in the glycosylation of BMPR1A and regulation of its protein stability (PubMed:29415997). Essential for extraembryonic development during early embryogenesis (PubMed:20574042, PubMed:21057870).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210040 | B4galt5 (untagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5), (10ug) |
CNY 3,990.00 |
|
| MG225689 | B4galt5 (tGFP-tagged) - Mouse UDP-Gal:betaGlcNAc beta 14-galactosyltransferase polypeptide 5 (B4galt5), (10ug) |
CNY 3,710.00 |
|
| MR225689L3 | Lenti ORF clone of B4galt5 (Myc-DDK-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5) |
CNY 5,230.00 |
|
| MR225689L4 | Lenti ORF clone of B4galt5 (mGFP-tagged) - Mouse UDP-Gal:betaGlcNAc beta 1,4-galactosyltransferase, polypeptide 5 (B4galt5) |
CNY 5,230.00 |
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