Lrat (NM_023624) Mouse Tagged ORF Clone
CAT#: MR225686
- TrueORF®
Lrat (Myc-DDK-tagged) - Mouse lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) (Lrat)
ORF Plasmid: tGFP
"NM_023624" in other vectors (4)
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CNY 2,081.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 1300010A18Rik; AI449251 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225686 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGAACCCAATGCTGGAAGCTGCGTCCCTCCTCCTGGAGAAACTGCTCCTTATTTCCAACTTCAAGC TCTTTAGCGTGAGCGTCCCGGGAGGAGGCACAGGGAAGAACCGTCCCTATGAAATCAGCTCTTTCGTCCG GGGCGACGTGTTGGAGGTGTCACGGACCCATTTTATCCACTATGGGATCTACCTGGGGGAAAACCGTGTC GCCCATCTAATGCCTGACATCCTGTTGGCCCTGACCAATGACAAGGAACGCACTCAGAAGGTGGTCTCCA ACAAGCGTCTCCTCCTGGGAGTCATTTGCAAGGTGGCTAGCATCCGTGTGGACACAGTAGAGGACTTTGC CTACGGAGCAGACATCCTAGTCAATCACCTAGACGGGACTCTCAAGAAGAAGTCCTTACTCAATGAGGAG GTGGCACGCAGAGCTGAGCAGCAGTTGGGACTGACTCCATACAGCCTACTGTGGAACAACTGCGAACACT TTGTGACTTACTGCAGATATGGCTCTCGGATCAGTCCACAGGCTGAGAAGTTCTATGACACCGTGAAGAT AATCATTCGTGATCAGAGAAGCAGTCTTGCTTCGGCTGTCTTGGGATTAGCATCTATTGTCTACACAGGC CTGGCATCATATATGACCCTTCCTGCAATCTGTATCCCATTCTGCTTGTGGATGATGTCTGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225686 protein sequence
Red=Cloning site Green=Tags(s) MKNPMLEAASLLLEKLLLISNFKLFSVSVPGGGTGKNRPYEISSFVRGDVLEVSRTHFIHYGIYLGENRV AHLMPDILLALTNDKERTQKVVSNKRLLLGVICKVASIRVDTVEDFAYGADILVNHLDGTLKKKSLLNEE VARRAEQQLGLTPYSLLWNNCEHFVTYCRYGSRISPQAEKFYDTVKIIIRDQRSSLASAVLGLASIVYTG LASYMTLPAICIPFCLWMMSG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_023624 |
ORF Size | 696 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_023624.4, NP_076113.1 |
RefSeq Size | 5349 bp |
RefSeq ORF | 696 bp |
Locus ID | 79235 |
UniProt ID | Q9JI60 |
MW | 25.8 kDa |
Gene Summary | Transfers the acyl group from the sn-1 position of phosphatidylcholine to all-trans retinol, producing all-trans retinyl esters (By similarity). Retinyl esters are storage forms of vitamin A (By similarity). LRAT plays a critical role in vision (By similarity). It provides the all-trans retinyl ester substrates for the isomerohydrolase which processes the esters into 11-cis-retinol in the retinal pigment epithelium; due to a membrane-associated alcohol dehydrogenase, 11 cis-retinol is oxidized and converted into 11-cis-retinaldehyde which is the chromophore for rhodopsin and the cone photopigments (By similarity). Required for the survival of cone photoreceptors and correct rod photoreceptor cell morphology (PubMed:25416279).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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LRAT Overexpression Diminishes Intracellular Levels of Biologically Active Retinoids and Reduces Retinoid Antitumor Efficacy in the Murine Melanoma B16F10 Cell Line
,Amann, PM;Czaja, K;Bazhin, AV;Rühl, R;Eichmüller, SB;Merk, HF;Baron, JM;,
Skin Pharmacol Physiol
,PubMed ID 25721651
[LRAT]
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Documents
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FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC211915 | Lrat (untagged) - Mouse lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) (Lrat), (10ug) |
CNY 3,990.00 |
|
MG225686 | Lrat (tGFP-tagged) - Mouse lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) (Lrat), (10ug) |
CNY 2,850.00 |
|
MR225686L3 | Lenti ORF clone of Lrat (Myc-DDK-tagged) - Mouse lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) (Lrat) |
CNY 4,750.00 |
|
MR225686L4 | Lenti ORF clone of Lrat (mGFP-tagged) - Mouse lecithin-retinol acyltransferase (phosphatidylcholine-retinol-O-acyltransferase) (Lrat) |
CNY 4,750.00 |