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Ephb2 (NM_010142) Mouse Tagged ORF Clone
CAT＃: MR225676
- TrueORF®
Ephb2 (Myc-DDK-tagged) - Mouse Eph receptor B2 (Ephb2)
ORF Plasmid: tGFP
"NM_010142" in other vectors (4)
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CNY 10,832.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Cek5; Dr; Drt; Er; Erk; ETECK; Hek5; Nu; Nuk; Prk; Prkm5; Qek5; Sek; Sek3; Tyr; Tyro5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225676 representing NM_010142
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGTGCGCAGGCTGGGGGCCGCGCTGCTGCTGCTGCCGCTGCTAGCCGCCGTGGAAGAAACCCTGA TGGACTCTACGACAGCAACGGCTGAGCTGGGCTGGATGGTACATCCCCCATCAGGGTGGGAAGAGGTGAG CGGCTACGACGAGAACATGAACACTATCCGTACCTACCAGGTGTGCAATGTCTTTGAGTCAAGCCAGAAC AACTGGCTGCGGACCAAATTCATCCGGCGCCGCGGCGCCCACCGCATCCACGTGGAGATGAAGTTCTCGG TGCGTGACTGCAGCAGCATTCCCAGCGTGCCGGGCTCCTGCAAGGAGACCTTCAACCTCTACTACTATGA GGCTGATTTTGACTTAGCCACCAAAACCTTTCCCAACTGGATGGAGAATCCGTGGGTGAAGGTGGACACC ATCGCGGCCGATGAGAGCTTCTCTCAGGTGGACCTGGGTGGCCGCGTCATGAAAATCAACACTGAGGTGC GAAGCTTCGGTCCCGTGTCCCGCAATGGTTTCTACCTGGCCTTCCAGGACTATGGCGGCTGTATGTCCCT CATTGCTGTGCGCGTCTTCTACCGGAAGTGCCCCCGAATCATCCAGAATGGTGCCATCTTCCAGGAGACA CTGTCGGGGGCTGAGAGCACTTCGCTGGTGGCAGCTCGGGGCAGCTGCATCGCCAATGCTGAAGAAGTGG ATGTGCCCATCAAACTCTACTGTAACGGGGACGGCGAATGGCTGGTGCCCATAGGTCGCTGCATGTGCAA GGCGGGCTTCGAGGCTGTGGAGAACGGCACCGTCTGCCGAGGTTGTCCATCAGGAACCTTCAAGGCCAAC CAAGGGGACGAAGCCTGCACCCACTGTCCCATCAACAGCCGCACCACCTCCGAGGGTGCCACCAACTGTG TATGCCGCAACGGCTACTACAGGGCCGACCTGGACCCCTTAGACATGCCTTGCACAACCATCCCCTCTGC GCCCCAGGCTGTGATCTCCAGCGTCAACGAGACGTCCCTCATGCTAGAGTGGACCCCACCCCGAGACTCG GGGGGTCGCGAGGATCTTGTTTACAACATCATCTGCAAGAGCTGTGGCTCCGGCCGGGGCGCATGCACGC GCTGCGGGGACAACGTGCAGTACGCGCCCCGCCAGCTGGGCCTGACTGAGCCGCGCATCTACATCAGTGA CCTGCTGGCACACACGCAGTACACCTTCGAGATCCAGGCCGTGAACGGTGTGACTGACCAGAGTCCCTTC TCACCTCAGTTCGCCTCTGTGAACATCACCACCAACCAAGCAGCACCATCGGCCGTGTCCATCATGCACC AGGTGAGCCGCACTGTGGACAGCATCACCCTGTCGTGGTCCCAGCCAGACCAGCCCAACGGTGTGATCCT GGACTACGAGCTGCAGTACTATGAGAAGGAGCTCAGTGAGTACAACGCCACGGCCATAAAAAGCCCCACC AACACAGTCACTGTGCAGGGCCTCAAAGCCGGCGCCATCTATGTCTTCCAGGTGCGGGCACGCACCGTTG CAGGCTATGGGCGCTACAGTGGCAAGATGTACTTCCAAACCATGACAGAAGCCGAGTACCAGACCAGCAT CAAGGAAAAGCTACCCCTCATCGTTGGCTCCTCCGCCGCCGGCTTAGTCTTCCTCATCGCTGTGGTCGTC ATTGCCATCGTATGTAACAGACGGGGGTTTGAGCGTGCCGACTCAGAGTACACGGACAAGCTACAACACT ACACCAGCGGACACATGACCCCAGGCATGAAGATCTATATAGACCCTTTCACCTATGAAGATCCTAATGA GGCAGTGCGGGAGTTTGCCAAGGAAATTGACATCTCCTGTGTCAAGATTGAGCAGGTGATCGGAGCAGGG GAATTTGGTGAGGTCTGCAGTGGCCATTTGAAGCTGCCAGGCAAGAGAGAGATCTTTGTAGCCATCAAGA CCCTCAAGTCAGGATACACGGAGAAACAGCGCCGGGACTTCCTGAGTGAGGCATCCATCATGGGCCAGTT CGACCACCCCAATGTCATCCATCTGGAAGGGGTTGTCACCAAGAGCACACCTGTCATGATCATCACTGAA TTCATGGAGAACGGATCTCTGGACTCCTTCCTCCGGCAAAACGATGGGCAGTTCACAGTCATCCAACTGG TGGGCATGCTGAGGGGCATTGCAGCCGGCATGAAGTACCTGGCGGACATGAACTACGTGCACCGTGACCT TGCTGCTCGAAACATCCTCGTCAACAGCAACCTGGTGTGTAAGGTGTCTGATTTTGGGCTCTCACGCTTC CTGGAGGATGACACGTCTGACCCCACCTATACCAGCGCTCTGGGTGGGAAGATCCCCATCCGTTGGACGG CACCGGAAGCCATCCAGTACCGGAAATTCACCTCGGCCAGTGATGTGTGGAGCTATGGCATCGTCATGTG GGAGGTGATGTCCTACGGGGAACGACCCTACTGGGACATGACCAATCAAGACGTAATCAACGCCATTGAA CAGGACTACAGACTACCTCCGCCCATGGACTGCCCTAGTGCCCTGCACCAGCTCATGCTGGACTGCTGGC AGAAGGACCGCAACCACCGGCCCAAGTTCGGCCAGATTGTCAACACGCTGGACAAGATGATCCGAAACCC CAACAGCCTCAAAGCCATGGCACCCCTGTCCTCTGGCATCAACCTGCCACTGCTGGACCGCACGATACCG GACTACACCAGCTTTAACACGGTGGATGAGTGGCTAGAGGCCATCAAGATGGGCCAGTACAAGGAGAGCT TTGCCAACGCCGGCTTTACCTCTTTCGACGTTGTATCTCAGATGATGATGGAGGACATTCTCCGCGTTGG GGTCACTCTAGCTGGCCACCAGAAAAAAATCCTGAACAGTATCCAGGTGATGCGGGCCCAGATGAACCAG ATCCAGTCTGTAGAGGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225676 representing NM_010142
Red=Cloning site Green=Tags(s) MAVRRLGAALLLLPLLAAVEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQN NWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVKVDT IAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQET LSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKAN QGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDS GGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPF SPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPT NTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEKLPLIVGSSAAGLVFLIAVVV IAIVCNRRGFERADSEYTDKLQHYTSGHMTPGMKIYIDPFTYEDPNEAVREFAKEIDISCVKIEQVIGAG EFGEVCSGHLKLPGKREIFVAIKTLKSGYTEKQRRDFLSEASIMGQFDHPNVIHLEGVVTKSTPVMIITE FMENGSLDSFLRQNDGQFTVIQLVGMLRGIAAGMKYLADMNYVHRDLAARNILVNSNLVCKVSDFGLSRF LEDDTSDPTYTSALGGKIPIRWTAPEAIQYRKFTSASDVWSYGIVMWEVMSYGERPYWDMTNQDVINAIE QDYRLPPPMDCPSALHQLMLDCWQKDRNHRPKFGQIVNTLDKMIRNPNSLKAMAPLSSGINLPLLDRTIP DYTSFNTVDEWLEAIKMGQYKESFANAGFTSFDVVSQMMMEDILRVGVTLAGHQKKILNSIQVMRAQMNQ IQSVEV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_010142 |
| ORF Size | 2958 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_010142.4, NP_034272.1 |
| RefSeq Size | 4780 bp |
| RefSeq ORF | 2961 bp |
| Locus ID | 13844 |
| UniProt ID | P54763 |
| MW | 110.3 kDa |
| Gene Summary | This gene encodes a member of the Eph receptor family of receptor tyrosine kinase transmembrane glycoproteins. These receptors consist of an N-terminal glycosylated ligand-binding domain, a transmembrane region and an intracellular kinase domain. The encoded receptor preferentially binds membrane-bound ephrin-B ligands and is involved in nervous system and vascular development. This gene is used as a marker of intestinal stem cells. Homozygous knockout mice for this gene exhibit impaired axon guidance and vestibular function. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222940 | Ephb2 (untagged) - Mouse Eph receptor B2 (Ephb2), (10ug) |
CNY 10,840.00 |
|
| MG225676 | Ephb2 (tGFP-tagged) - Mouse Eph receptor B2 (Ephb2), (10ug) |
CNY 10,830.00 |
|
| MR225676L3 | Lenti ORF clone of Ephb2 (Myc-DDK-tagged) - Mouse Eph receptor B2 (Ephb2) |
CNY 11,780.00 |
|
| MR225676L4 | Lenti ORF clone of Ephb2 (mGFP-tagged) - Mouse Eph receptor B2 (Ephb2) |
CNY 11,780.00 |
