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Nek10 (NM_001195229) Mouse Tagged ORF Clone
CAT＃: MR225666
- TrueORF®
Nek10 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)- related kinase 10 (Nek10)
ORF Plasmid: tGFP
"NM_001195229" in other vectors (4)
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CNY 7,768.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Gm282 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225666 representing NM_001195229
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCGATCAAGATACAAAGGCGAAGAGCACAGAGAAAACCGCTGATAAGCAACAAGGGACCACCACCA GGGACTATTCAGATCTTAAAAGACTTCGGTGCCTGTTGAACGTTCAGTCAAGCAAACAGCAGCTTCCAGC CATTAACTTTGACAGTGCCCAAAATAACATGACAAAGTCTGAGCCAACCATCAGGACAGGGGGACATAGA GCTCGTGGTCAATGGCATGAGTCCACAGAAGCAGTTGAACTTGAAAATTTTAGTATAAACTATAAGAATG AAAGGAATTTCAGCAAACATCCACAGCATCAGTTATTTCAAGAGATCTTCACAGCTCTGGTGAGAAACAG ACTTATATGCAGAGAGTGGGTTAATCGTGCTCCATCCATTCATTTTCTGAGAGTGTTAATTTGTCTGAGG CTGCTGATGCGGGATCCATGTTATCAGGAAATACTCCATAAATTGGGTGGTATTGAAGACCTTGCTCAGT ACATGGAGATCGTGGCCAACGAGTACCTGGGCTACGCTGAGGAGCAGCACTGTGTGGACAAGCTGGTCAA TATGACGTATATTTTTCAAAAACTTGCTGCTGTGAAAGACCAAAGAGAATGGGTTACTGCAAGTGGAGCC CACAAGACATTAGTGAGTTTGCTTGGTGCCCGAGACACCACTGTCCTCTTGGGTGCTCTGCTGGCACTGG CCAGCTTAGCAGAGAGTTCAGAGTGTAGGGAGAAGATCAGTGAACTCAACGTAGTAGAAAATCTGTTGAT GATTCTGCACGAATATGATTTACTCTCCAAAAGACTCACTGCCGAGCTCCTGCGCCTGCTTTGTGCTGAG CCCCAGATCAAGGAGCAGGTGAAACTCTATGAAGGGATCCCCATCCTGCTCAGTCTGCTTCATTCTGATC ACTTGAAGCTGCTGTGGAGTGTGATCTGGATTCTGGTCCAGGTGTGTGAGGACCCGGAGACTAGTGTGGA AATCCGAATCTGGGGTGGCATCAAGCAGCTCCTTCATATTTTACAAGGAGACAGAAATTTTGTTTCTGAC CGTTCTTCCATCGGAAGCCTGTCCAGTGCAAATGCCGCAGGACGCATCCAGCAGCTTCATTTGTCAGAAG ATTTAAGCCCTGGGGAAATCGAGGAAAATACTGTCTCTCTCCAAGCAGCCTGCTGTGCTGCCCTCACCGA GCTGGCCCTCAATGATACCAATGCCCACCAAGTGGTACAGGAAAATGGTGTATATACAATAGCAAAATTA ATTTTACCAAATAAACAATCGAATGCAGCACAAACTAATCTACTACAGTGTTATGCTTTCAGAACCCTGA GGTTTCTCTTCAGTATGGAAAGGAACAGGCCACTCTTTAAAAGACTTTTTCCCACCGACCTGTTTGAGAC CTTCATTGACATAGGACATTACGTTCGTGATATTGGTGCTTATAAGGACTTGGTATCACAGTTGAATTTA CTATTGGAAGATGAGCTGAAGCAAATTGCTGAGAATATTGAAAGCATCAATCAGAAGAAAGCGCCCTTGA AGTACATAGGTGACTATGCTGTGTTGGACCACCTTGGAAGTGGAGCTTTTGGCTGTGTTTATAAGGTTAG AAAGCGTAGTGGTCAAAATCTTTTAGCAATGAAGGAGGTCAATTTGCATAACCCAGCATTTGGAAAGGAT AAGAAAGACCGAGACAGCAGTGTCAAGAACATTGTTTCTGAATTAACAATAATTAAGGAGCAGCTTTATC ATCCCAATGTTGTACGTTATTATAAAACATTTTTGGAAAATGACAGGTTGTATATCGTTATGGAGCTGAT AGAAGGAGCCCCACTTGGAGAGCACTTTAATTCTTTGAAGGAAAAACATCACCATTTCAGTGAAGAGCGA CTGTGGAAAATATTTATACAGCTGTGCTTAGCTCTTCGGTATCTGCACAAGGAGAAGAGGATTGTTCACA GAGATCTCACGCCAAACAACATTATGCTGGGAGATAAGGACAAAGTGACAGTTACTGACTTTGGCCTGGC AAAGCAAAAACAGGAAAGCAGTAAGCTTACATCTATGGTGGGAACGATCCTCTATTCTTGCCCAGAGGTC CTGAAGAGTGAGCCATATGGAGAGAAGGCCGATGTCTGGGCTGCTGGCTGCATCCTTTATCAGATGGCGA CTCTAAGTCCTCCCTTCTGTAGCACCAACATGCTCTCCTTGGCTACTAAAATAGTGGAGGCTGTATATGA GCCAGTGCCAGAAGGTATCTACTCTGAAAAAGTGACAGATACCATTAGAAGGTGCCTCACTCCTGATGCA GAAGCCCGTCCAGATATTGTAGAAGTTAGTTCCATGATATCAGATGTCATGATGAAGTATTTAGACAGAT TATCCACATCCCAGCTAGCCTTGGAGAGGAAACTGGAAAGGGAGCGGAGGCGCACACAGAGATACTTTAT GGAAGCCAACAGGAATGCTGTCACATGTCACCATGAGCTGGCTCTTCTGTCCCAGGAGACCTTTGAGAAG GCAAGCCTGAGCAGCAGTAGCAGCGGGGCAGCCAGCCTGAAAAGTGAGCTTTCAGAGAGCGCAGAGCTGC CTGGAGAGGGCTGCCACATCCCCTGCGGGAAGGAGGAGGACAGGGTCTGTGAGGAGGTGCTGTCAGAAGA CAACTTCCAGCTGGAGAGTGTGGAGAAAGACCTGTATTCTGAGCTAGACGATGAGCTGGACGTCTCGGAC AACTGTAGCAGCTCCAGTTCAAGCCCCCTAAAGGAGTCTACATTCAGCATTTTAAAGCGAAGTTTCAGTG CTTCGGGAAGAGAAAGGCACTCACAGGCAAGGGACTTCATTGCTGGACTAGGATCAAGACCAAGACCAGC ATCTGCAGGCATCGCTGTATCCCAGCGGAAAGTGCGGCAGATCTGCGACCCCATTCAGCAGATCCTGATC CAGCTACATAAAGTTATCTACATCACGCAGCTCCCTCCAGCTCTGCACCATGATCTGAAGAGAAGGGTTA TAGAGAGATTCAAGAAGTCCCTGTTCAGTCAGCAGAGCAACCCATGTAACCTGAAATCTGAAATTAAGAA GTTGTCTCAGGGGTCTCCAGAACCCATCGAGCTGAACTTTCTTACCTCAGATTATCATTCACTTCGCCAT TCACGAGCTGCAAACAACTGGTCCCCAAGTGACCCCACAGGTTCACCCAGCAGCTTTGAAGTGGAAGAGG GAGTCACATATGAGCAGATGCAGACTGTGATTGAAGAAGTCCTAGAGGAAAGTGGCTATTACAACTTTAC AACTAAGAGGTGTCACTCTTTTCCATGGGTGACCAAGAGCTACACAGCCAAAAGA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225666 representing NM_001195229
Red=Cloning site Green=Tags(s) MPDQDTKAKSTEKTADKQQGTTTRDYSDLKRLRCLLNVQSSKQQLPAINFDSAQNNMTKSEPTIRTGGHR ARGQWHESTEAVELENFSINYKNERNFSKHPQHQLFQEIFTALVRNRLICREWVNRAPSIHFLRVLICLR LLMRDPCYQEILHKLGGIEDLAQYMEIVANEYLGYAEEQHCVDKLVNMTYIFQKLAAVKDQREWVTASGA HKTLVSLLGARDTTVLLGALLALASLAESSECREKISELNVVENLLMILHEYDLLSKRLTAELLRLLCAE PQIKEQVKLYEGIPILLSLLHSDHLKLLWSVIWILVQVCEDPETSVEIRIWGGIKQLLHILQGDRNFVSD RSSIGSLSSANAAGRIQQLHLSEDLSPGEIEENTVSLQAACCAALTELALNDTNAHQVVQENGVYTIAKL ILPNKQSNAAQTNLLQCYAFRTLRFLFSMERNRPLFKRLFPTDLFETFIDIGHYVRDIGAYKDLVSQLNL LLEDELKQIAENIESINQKKAPLKYIGDYAVLDHLGSGAFGCVYKVRKRSGQNLLAMKEVNLHNPAFGKD KKDRDSSVKNIVSELTIIKEQLYHPNVVRYYKTFLENDRLYIVMELIEGAPLGEHFNSLKEKHHHFSEER LWKIFIQLCLALRYLHKEKRIVHRDLTPNNIMLGDKDKVTVTDFGLAKQKQESSKLTSMVGTILYSCPEV LKSEPYGEKADVWAAGCILYQMATLSPPFCSTNMLSLATKIVEAVYEPVPEGIYSEKVTDTIRRCLTPDA EARPDIVEVSSMISDVMMKYLDRLSTSQLALERKLERERRRTQRYFMEANRNAVTCHHELALLSQETFEK ASLSSSSSGAASLKSELSESAELPGEGCHIPCGKEEDRVCEEVLSEDNFQLESVEKDLYSELDDELDVSD NCSSSSSSPLKESTFSILKRSFSASGRERHSQARDFIAGLGSRPRPASAGIAVSQRKVRQICDPIQQILI QLHKVIYITQLPPALHHDLKRRVIERFKKSLFSQQSNPCNLKSEIKKLSQGSPEPIELNFLTSDYHSLRH SRAANNWSPSDPTGSPSSFEVEEGVTYEQMQTVIEEVLEESGYYNFTTKRCHSFPWVTKSYTAKR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001195229 |
| ORF Size | 3345 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001195229.1, NP_001182158.1 |
| RefSeq Size | 3348 bp |
| RefSeq ORF | 3348 bp |
| Locus ID | 674895 |
| UniProt ID | Q3UGM2 |
| MW | 126.7 kDa |
| Gene Summary | Plays a role in the cellular response to UV irradiation. Mediates G2/M cell cycle arrest, MEK autoactivation and ERK1/2-signaling pathway activation in response to UV irradiation.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The E3 ubiquitin ligase RNF114 and TAB1 degradation are required for maternal-to-zygotic transition
,Yang, Y;Zhou, C;Wang, Y;Liu, W;Liu, C;Wang, L;Liu, Y;Shang, Y;Li, M;Zhou, S;Wang, Y;Zeng, W;Zhou, J;Huo, R;Li, W;,
EMBO Rep
,PubMed ID 28073917
[Nek10]
|
Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223626 | Nek10 (untagged) - Mouse NIMA (never in mitosis gene a)- related kinase 10 (Nek10), (10ug) |
CNY 14,250.00 |
|
| MG225666 | Nek10 (tGFP-tagged) - Mouse NIMA (never in mitosis gene a)- related kinase 10 (Nek10), (10ug) |
CNY 10,740.00 |
|
| MR225666L3 | Lenti ORF clone of Nek10 (Myc-DDK-tagged) - Mouse NIMA (never in mitosis gene a)- related kinase 10 (Nek10) |
CNY 11,690.00 |
|
| MR225666L4 | Lenti ORF clone of Nek10 (mGFP-tagged) - Mouse NIMA (never in mitosis gene a)- related kinase 10 (Nek10) |
CNY 11,690.00 |
