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Sun2 (NM_194342) Mouse Tagged ORF Clone
CAT＃: MR225632
- TrueORF®
Sun2 (Myc-DDK-tagged) - Mouse Sad1 and UNC84 domain containing 2 (Sun2), transcript variant 3
ORF Plasmid: tGFP
"NM_194342" in other vectors (4)
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CNY 5,120.00
CNY 6,180.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B230369L08Rik; C030011B15; Unc84b |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225632 representing NM_194342
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAGACGAAGCCAGCGCCTCACTCGCTACTCTCAGGATGATAACGATGGCGGCAGCAGCAGCAGTG GTGCGAGCTCCGTGGCAGGAAGCCAGGGCACCGTGTTTAAAGACAGTCCTCTCAGGACTTTGAAGAGGAA ATCCAGCAACATGAAGCACCTGTCCCCAGCTCCACAGCTGGGCCCCTCCTCTGACTCCCACACCTCCTAC TACAGCGAGTCTGTGGTTCGAGAGTCCTACATCGGCAGCCCCCGGGCTGTGTCCCTCGCCAGGAGTGCCC TCCTGGATGACCACCTACACAGTGAGCCCTACTGGAGCGGGGACCTTCGGGGGAGGAGGAGGAGAGGAAC AGGTGGTTCTGAGAGCAGCAAGGCCAATGGGCTCACCGCGGAGAGCAAGGCCTCAGAAGACTTTTTCGGA TCTTCCTCAGGCTATTCTTCAGAGGATGACCTTGCAGGCCGCCTCTTTGGTCTTCTCTACTGGTGGATTG GCACCACCTGGTACCGCCTGACAACTGCTGCCTCCCTCCTGGATGTCTTCGTCCTAACCAGGTCCAGGCA CTTCTCGCTGAACCTGAAGAGTTTTCTGTGGTTCCTTCTGCTCTTGCTACTCCTGACTGGTCTGACCTAC GGTGCTTGGCATTTTTACCCCTTAGGGCTGCAGACATTGCAACCCGCTGTGGTCTCCTGGTGGGCAGCAA AAGAGAGCAGGAAGCAGCCAGAGGTGTGGGAATCCAGAGACGCCTCCCAGCACTTCCAGGCTGAGCAGCG CGTTCTCTCCCGGGTTCACTCTCTGGAGCGGCGTCTGGAAGCCCTTGCTGCAGACTTTTCCTCCAACTGG CAGAAGGAGGCCATACGGCTGGAACGCCTGGAGCTGCGGCAGGGGGCTGCTGGCCATGGAGGAGGCAGTA GCCTGAGCCATGAAGATGCCCTGTCTCTCCTAGAAGGGTTGGTGAGCCGCCGCGAGGCTACCCTGAAGGA GGACTTGCGCAGGGACACAGTGGCTCATATCCAGGAAGAATTGGCTACCCTGAGGGCAGAGCATCACCAA GACTCGGAAGATCTCTTCAAGAAGATCGTCCAGGCCTCTCAGGAGTCCGAAGCCCGAGTCCAGCAGCTGA AGACAGAATGGAAAAGCATGACCCAGGAGGCCTTCCAGGAGAGCTCTGTGAAGGAGCTGGGACGGCTGGA AGCCCAGCTGGCCAGCCTGCGGCAGGAGCTGGCTGCCCTGACTCTGAAGCAGAACTCGGTGGCAGATGAA GTGGGCCTGCTGCCACAGAAGATCCAGGCTGCCAGGGCTGATGTGGAATCCCAGTTCCCTGACTGGATCA GGCAGTTCCTTCTTGGAGACAGGGGTGCGCGCAGCGGGCTCCTGCAGAGAGATGAGATGCACGCTCAGCT GCAGGAGCTGGAGAACAAGATCCTTACCAAGATGGCTGAGATGCAGGGCAAGTCAGCCAGGGAGGCCGCA GCGTCCCTGGGACAGATACTGCAGAAAGAAGGCATAGTTGGGGTGACAGAGGAGCAGGTGCACCGGATCG TCAAGCAGGCCCTGCAGCGCTACAGTGAGGACAGGATTGGAATGGTGGATTACGCCCTGGAATCAGGAGG AGCCAGTGTTATCAGCACCCGCTGCTCTGAGACTTATGAGACCAAGACGGCACTCCTCAGCCTCTTTGGC ATCCCCCTGTGGTACCACTCCCAGTCACCTCGGGTCATTCTGCAGCCAGATGTGCACCCAGGCAACTGCT GGGCCTTCCAGGGGCCCCAGGGCTTTGCAGTGGTCCGCCTCTCTGCTCGAATCCGACCTACAGCCGTTAC CTTAGAGCACGTGCCCAAGGCCTTGTCACCCAACAGCACTATCTCCAGTGCTCCCAAGGACTTCGCCATC TTTGGCTTCGATGAAGACCTGCAGCAGGAAGGGACACTTCTTGGCACGTTTGCCTACGACCAGGATGGGG AGCCCATCCAGACCTTCTATTTCCAGGCCTCTAAGATGGCCACATACCAAGTTGTGGAGCTTCGGATCCT GACCAACTGGGGCCACCCTGAGTACACGTGTATCTACCGCTTCCGGGTGCACGGAGAGCCTGCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225632 representing NM_194342
Red=Cloning site Green=Tags(s) MSRRSQRLTRYSQDDNDGGSSSSGASSVAGSQGTVFKDSPLRTLKRKSSNMKHLSPAPQLGPSSDSHTSY YSESVVRESYIGSPRAVSLARSALLDDHLHSEPYWSGDLRGRRRRGTGGSESSKANGLTAESKASEDFFG SSSGYSSEDDLAGRLFGLLYWWIGTTWYRLTTAASLLDVFVLTRSRHFSLNLKSFLWFLLLLLLLTGLTY GAWHFYPLGLQTLQPAVVSWWAAKESRKQPEVWESRDASQHFQAEQRVLSRVHSLERRLEALAADFSSNW QKEAIRLERLELRQGAAGHGGGSSLSHEDALSLLEGLVSRREATLKEDLRRDTVAHIQEELATLRAEHHQ DSEDLFKKIVQASQESEARVQQLKTEWKSMTQEAFQESSVKELGRLEAQLASLRQELAALTLKQNSVADE VGLLPQKIQAARADVESQFPDWIRQFLLGDRGARSGLLQRDEMHAQLQELENKILTKMAEMQGKSAREAA ASLGQILQKEGIVGVTEEQVHRIVKQALQRYSEDRIGMVDYALESGGASVISTRCSETYETKTALLSLFG IPLWYHSQSPRVILQPDVHPGNCWAFQGPQGFAVVRLSARIRPTAVTLEHVPKALSPNSTISSAPKDFAI FGFDEDLQQEGTLLGTFAYDQDGEPIQTFYFQASKMATYQVVELRILTNWGHPEYTCIYRFRVHGEPAH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_194342 |
| ORF Size | 2097 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_194342.3, NP_919323.2 |
| RefSeq Size | 3711 bp |
| RefSeq ORF | 2100 bp |
| Locus ID | 223697 |
| UniProt ID | Q8BJS4 |
| MW | 78.6 kDa |
| Gene Summary | As a component of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex, involved in the connection between the nuclear lamina and the cytoskeleton. The nucleocytoplasmic interactions established by the LINC complex play an important role in the transmission of mechanical forces across the nuclear envelope and in nuclear movement and positioning. Specifically, SYNE2 and SUN2 assemble in arrays of transmembrane actin-associated nuclear (TAN) lines which are bound to F-actin cables and couple the nucleus to retrograde actin flow during actin-dependent nuclear movement. Required for interkinetic nuclear migration (INM) and essential for nucleokinesis and centrosome-nucleus coupling during radial neuronal migration in the cerebral cortex and during glial migration. Required for nuclear migration in retinal photoreceptor progenitors implicating association with cytoplasmic dynein-dynactin and kinesin motor complexes, and probably B-type lamins; SUN1 and SUN2 seem to act redundantly. The SUN1/2:KASH5 LINC complex couples telomeres to microtubules during meiosis; SUN1 and SUN2 seem to act at least partial redundantly. Anchors chromosome movement in the prophase of meiosis and is involved in selective gene expression of coding and non-coding RNAs needed for gametogenesis. Required for telomere attachment to nuclear envelope and gametogenesis. May also function on endocytic vesicles as a receptor for Rab5-GDP and participate in the activation of Rab5.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
The SUN2-nesprin-2 LINC complex and KIF20A function in the Golgi dispersal
,Hieda, M;Matsumoto, T;Isobe, M;Kurono, S;Yuka, K;Kametaka, S;Wang, JY;Chi, YH;Kameda, K;Kimura, H;Matsuura, N;Matsuura, S;,
Scientific reports
,PubMed ID 33686165
[SUN2]
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Documents
| Product Manuals |
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC220620 | Sun2 (untagged) - Mouse Sad1 and UNC84 domain containing 2 (Sun2), transcript variant 3, (10ug) |
CNY 9,030.00 |
|
| MG225632 | Sun2 (tGFP-tagged) - Mouse Sad1 and UNC84 domain containing 2 (Sun2), (10ug) |
CNY 6,720.00 |
|
| MR225632L3 | Lenti ORF clone of Sun2 (Myc-DDK-tagged) - Mouse Sad1 and UNC84 domain containing 2 (Sun2), transcript variant 3 |
CNY 8,080.00 |
|
| MR225632L4 | Lenti ORF clone of Sun2 (mGFP-tagged) - Mouse Sad1 and UNC84 domain containing 2 (Sun2), transcript variant 3 |
CNY 8,080.00 |
