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Mcoln3 (NM_134160) Mouse Tagged ORF Clone
CAT＃: MR225394
- TrueORF®
Mcoln3 (Myc-DDK-tagged) - Mouse mucolipin 3 (Mcoln3)
ORF Plasmid: tGFP
"NM_134160" in other vectors (4)
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CNY 6,176.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 6720490O21Rik; TRPML3; Va |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225394 representing NM_134160
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAATCCCGAGGTGCTGGTTAGCAGCTGCAGAGCTCGCCAAGATGAAAGCCCCTGCACTTTCCACC CGAGCTCGTCCCCGTCAGAGCAGCTTCTCTTAGAAGACCAGATGAGGCGGAAACTCAAGTTCTTTTTTAT GAATCCTTGTGAGAAGTTCTGGGCTCGGGGTAGGAAGCCATGGAAACTTGCCATACAGATTCTGAAAATC GCGATGGTGACTATCCAGCTGGTTCTGTTTGGACTAAGTAACCAGATGGTAGTAGCTTTCAAAGAGGAGA ACACTATAGCCTTCAAACACCTCTTCCTAAAGGGCTACATGGATCGAATGGACGACACCTATGCAGTGTA CACTCAGAGTGAAGTGTATGACCAGATCATCTTTGCAGTGACCCAGTACTTGCAGCTTCAGAACATCTCC GTGGGCAATCACGCTTATGAGAACAAGGGGACTAAGCAGTCGGCGATGGCAATCTGTCAGCACTTCTACA GGCAAGGAACCATCTGCCCCGGGAACGACACCTTTGACATCGATCCAGAAGTTGAAACAGAATGTTTCCT TGTAGAGCCAGATGAAGCTTCCCACCTTGGAACGCCTGGAGAAAATAAACTCAACCTGAGCCTGGACTTC CACAGACTTCTGACGGTGGAGCTCCAGTTTAAGCTCAAAGCCATCAATCTGCAGACAGTTCGACACCAGG AGCTTCCTGACTGTTACGACTTTACGCTGACTATAACATTCGACAACAAGGCTCACAGTGGAAGAATCAA AATAAGCTTAGACAACGACATTTCTATCAAAGAATGCAAAGACTGGCATGTGTCTGGATCAATTCAGAAG AACACACACTACATGATGATCTTTGATGCCTTTGTCATTCTGACCTGCTTGGCCTCACTGGTGCTGTGTG CCAGGTCTGTGATTAGGGGTCTTCAGCTTCAGCAGGAGTTTGTCAACTTCTTCCTTCTTCACTACAAGAA GGAAGTTTCGGCCTCTGATCAGATGGAGTTCATCAACGGGTGGTACATTATGATCATCATTAGTGACATA TTGACAATCGTTGGATCAGTTCTGAAAATGGAAATCCAAGCCAAGAGTCTCACAAGCTATGATGTCTGCA GCATACTTCTCGGGACGTCAACTATGCTCGTGTGGCTTGGAGTTATCCGATACCTGGGTTTCTTTGCGAA GTACAATCTCCTTATTCTGACCCTCCAGGCAGCGCTGCCCAACGTCATGAGGTTCTGTTGCTGCGCTGCT ATGATCTATCTAGGCTATTGCTTTTGCGGATGGATTGTGCTGGGCCCTTACCATGAGAAGTTCCGTTCCC TGAACAGGGTCTCCGAGTGCCTGTTCTCGCTGATAAACGGAGACGATATGTTTTCCACATTTGCGAAAAT GCAGCAGAAGAGTTACCTGGTGTGGCTGTTCAGCCGAGTCTACCTGTACTCGTTCATCAGCCTCTTCATT TACATGATTCTGAGCCTTTTCATCGCGCTCATCACAGACACATACGAAACAATTAAGCACTACCAGCAAG ATGGCTTCCCAGAGACGGAACTTCGAAAGTTTATAGCGGAATGCAAAGACCTCCCCAACTCCGGAAAATA CAGATTAGAAGATGACCCTCCGGGTTCTTTACTCTGCTGCTGCAAAAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225394 representing NM_134160
Red=Cloning site Green=Tags(s) MANPEVLVSSCRARQDESPCTFHPSSSPSEQLLLEDQMRRKLKFFFMNPCEKFWARGRKPWKLAIQILKI AMVTIQLVLFGLSNQMVVAFKEENTIAFKHLFLKGYMDRMDDTYAVYTQSEVYDQIIFAVTQYLQLQNIS VGNHAYENKGTKQSAMAICQHFYRQGTICPGNDTFDIDPEVETECFLVEPDEASHLGTPGENKLNLSLDF HRLLTVELQFKLKAINLQTVRHQELPDCYDFTLTITFDNKAHSGRIKISLDNDISIKECKDWHVSGSIQK NTHYMMIFDAFVILTCLASLVLCARSVIRGLQLQQEFVNFFLLHYKKEVSASDQMEFINGWYIMIIISDI LTIVGSVLKMEIQAKSLTSYDVCSILLGTSTMLVWLGVIRYLGFFAKYNLLILTLQAALPNVMRFCCCAA MIYLGYCFCGWIVLGPYHEKFRSLNRVSECLFSLINGDDMFSTFAKMQQKSYLVWLFSRVYLYSFISLFI YMILSLFIALITDTYETIKHYQQDGFPETELRKFIAECKDLPNSGKYRLEDDPPGSLLCCCKK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_134160 |
| ORF Size | 1659 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_134160.1, NP_598921.1 |
| RefSeq Size | 1712 bp |
| RefSeq ORF | 1662 bp |
| Locus ID | 171166 |
| UniProt ID | Q8R4F0 |
| MW | 64.2 kDa |
| Gene Summary | Nonselective ligand-gated cation channel probably playing a role in the regulation of membrane trafficking events. Acts as Ca(2+)-permeable cation channel with inwardly rectifying activity (PubMed:17989217). Mediates release of Ca(2+) from endosomes to the cytoplasm, contributes to endosomal acidification and is involved in the regulation of membrane trafficking and fusion in the endosomal pathway (By similarity). Does not seem to act as mechanosensory transduction channel in inner ear sensory hair cells. Proposed to play a critical role at the cochlear stereocilia ankle-link region during hair-bundle growth (PubMed:18801844). Involved in the regulation of autophagy. Through association with GABARAPL2 may be involved in autophagosome formation possibly providing Ca(2+) for the fusion process (PubMed:24269818). Through a possible and probably tissue-specific heteromerization with MCOLN1 may be at least in part involved in many lysosome-dependent cellular events. Possible heteromeric ion channel assemblies with TRPV5 show pharmacological similarity with TRPML3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218873 | Mcoln3 (untagged) - Mouse mucolipin 3 (Mcoln3), (10ug) |
CNY 7,030.00 |
|
| MG225394 | Mcoln3 (tGFP-tagged) - Mouse mucolipin 3 (Mcoln3), (10ug) |
CNY 6,080.00 |
|
| MR225394L3 | Lenti ORF clone of Mcoln3 (Myc-DDK-tagged) - Mouse mucolipin 3 (Mcoln3) |
CNY 7,410.00 |
|
| MR225394L4 | Lenti ORF clone of Mcoln3 (mGFP-tagged) - Mouse mucolipin 3 (Mcoln3) |
CNY 7,410.00 |
