Pkn2 (NM_178654) Mouse Tagged ORF Clone
CAT#: MR225284
- TrueORF®
Pkn2 (Myc-DDK-tagged) - Mouse protein kinase N2 (Pkn2)
ORF Plasmid: tGFP
"NM_178654" in other vectors (4)
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CNY 7,200.00
CNY 8,650.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 6030436C20Rik; AI507382; PRK2; Prkcl2; Stk7 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR225284 representing NM_178654
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGTCCAACCCCGACCGGGGGGAGATCCTCCTCACCGAGCTGCAGGGAGATTCCCGGACTCTTCCAT TTTCTGAGAATGTGAGTGCTGTTCAGAAATTAGACTTTTCAGATACAATGGTGCAACAGAAATTGGACGA CATCAAGGATCGAATAAAGAGAGAAATAAGGAAAGAACTGAAAATCAAAGAAGGAGCTGAAAACCTGAGG AAAGTTACAACAGATAAGAAAAATTTGGCTTATGTAGACAACATTTTGAAAAAATCAAATAAAAAATTAG AAGAATTACATCACAAGCTACAAGAATTAAATGCACACATCGTTGTATCGGATCCAGAGGACTCCACAGA TTGTCCGAGGACTCCAGATACTCCAAATAGTGACTCTCGTAGTTCTACTAGCAACAATAGACTGATGGCC TTACAGAAACAACTGGATATAGAACTTAAAGTAAAGCAGGGTGCAGAGAACATGATACAGATGTACTCAA ATGGCTCTTCAAAGGATCGGAAACTCCATGGTACAGCGCAGCAACTGCTACAGGATAGCAAGACAAAAAT AGAAGTCATCCGGATGCAGATTCTTCAGGCCGTCCAGACCAATGAGCTGGCTTTTGATAATGCAAAACCT GTGATAAGCCCTCTTGAGCTTCGGATGGAAGAATTAAGACATCATTTTAAGATAGAATTTGCAGTAGCCG AAGGTGCAAAGAATGTCATGAAGTTACTTGGCTCAGGAAAAGTAACAGACAGAAAAGCGCTTTCAGAAGC ACAAGCGAGATTTAATGAATCAAGTCAGAAGTTGGACCTTTTAAAGTATTCACTAGAGCAAAGGTTAAAT GAGCTCCCCAGGAATCACCCCAAAAGCAGTGTCGTCATTGAGGAGCTTTCACTGGTTGCGTCACCAACCC TGAGTCCACGGCAGAGCATGCTGTCCACGCAGAACCAGTATAGCACACTGTCCAAGCCAGCGGCGCTAAC CGGTACTTTGGAAGTTCGTCTTATGGGGTGCCAAGATATCCTAGAGAATGTACCTGGACGGTCAAAAGCA ACGTCGGTAGCACTACCTGGGTGGAGTCCAAGTGACAACAGATCGTCTTTTATGAGCAGAACCAGCAAGA GTAAAAGTGGGAGTAGTCGCAACCTCCTGAAAACGGATGACTTGTCCAATGATGTTTGTGCTGTTTTGAA GCTAGATAATACTGTGGTTGGTCAAACTAGCTGGAAACCCATTTCAAATCAGTCGTGGGACCAGAAGTTT ACACTGGAACTAGACAGGTCACGTGAACTGGAAATTTCAGTTTATTGGCGTGATTGGCGATCTCTGTGTG CTGTAAAATTTCTGAGGTTAGAAGATTTTTTAGACAACCAGCGGCATGGCATGTGTCTCTATTTGGAACC ACAGGGTACTTTGTTTGCAGAGGTTACTTTTTTTAATCCAGTTATTGAAAGACGACCAAAACTCCAAAGA CAAAAGAAAATTTTTTCAAAACAACAAGGCAAAACATTTCTCAGAGCTCCTCAGATGAATATTAATATTG CCACTTGGGGAAGACTAGTGAGACGAGCTATTCCCACAGTAAATCATTCTGGCACATTCAGCCCTCAGAC TCCTGTGCCTGCAACAGTGCCAGTCGTTGACGCACGCATTCCTGATCTAGCACCTCCGGCCAGTGACTCT ACAGTAACCAAATTGGACTTTGATCTTGAACCTGAACCTCCTCCAGCCCCACCTCGTGCTTCTTCCCTTG GAGAGACAGATGAATCTTCTGAATTGAGAGTTTTGGATATACCTGGACAGGGTTCAGAAACTGTTTTCAA TATTGAGAATGACAGAAATAACCTGCGTCCAAAATCCAAATCTGAATATGAGCTGAGCATTCCTGACTCA GGTCGAAGCTGCTGGGGTGTTGGAGAGCTTGATGACAAGAGAGCTCAGCAGAGGTTTCAGTTCAGTCTAC AGGACTTCAGGTGCTGTGCTGTCTTGGGGAGAGGACACTTTGGAAAGGTGCTGTTGGCGGAATATAAACA CACAAATGAAATGTTTGCAATAAAAGCCTTAAAGAAAGGAGACATTGTGGCACGAGATGAAGTAGACAGC CTGATGTGTGAAAAAAGAATTTTTGAGACTGTGAATAGTGTCAGGCACCCGTTTCTAGTGAACCTTTTTG CATGTTTCCAAACAAAAGAACATGTTTGCTTCGTGATGGAGTATGCTGCAGGTGGTGACCTGATGATGCA CATCCATACTGATGTCTTCTCTGAACCCAGAGCTGTATTTTATGCAGCATGTGTAGTTCTTGGGTTGCAA TACTTACATGAACACAAAATTGTTTATAGAGATTTGAAGTTGGATAACTTGTTGCTAGATACAGAAGGCT TTGTGAAAATTGCTGACTTTGGTCTTTGCAAAGAAGGAATGGGGTATGGAGATAGGACAAGCACATTTTG TGGTACTCCTGAGTTTCTTGCCCCAGAAGTCCTAACAGAAACATCGTACACAAGGGCTGTAGATTGGTGG GGCCTTGGTGTACTTATATATGAGATGCTCGTCGGCGAGTCTCCCTTTCCTGGTGATGATGAAGAAGAGG TTTTTGACAGTATTGTAAATGACGAAGTAAGGTATCCAAGGTTCTTATCTACAGAAGCCATTTCCATAAT GAGAAGGCTGCTAAGGAGAAATCCTGAGCGGCGCCTGGGAGCTGGTGAGAAGGATGCAGAGGATGTCAAG AAGCACCCGTTTTTCCGGCTAACCGACTGGAGTGCTCTAATGGACAAAAAAGTAAAGCCACCATTTGTAC CTACCATTAGAGGACGGGAAGATGTTAGTAACTTTGATGATGAATTTACCTCAGAAGCACCTATTCTGAC TCCACCTCGAGAACCAAGGATACTCTTGGAAGAGGAGCAGGAGATGTTCCACGATTTTGACTACGTTGCT GATTGGTGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225284 representing NM_178654
Red=Cloning site Green=Tags(s) MASNPDRGEILLTELQGDSRTLPFSENVSAVQKLDFSDTMVQQKLDDIKDRIKREIRKELKIKEGAENLR KVTTDKKNLAYVDNILKKSNKKLEELHHKLQELNAHIVVSDPEDSTDCPRTPDTPNSDSRSSTSNNRLMA LQKQLDIELKVKQGAENMIQMYSNGSSKDRKLHGTAQQLLQDSKTKIEVIRMQILQAVQTNELAFDNAKP VISPLELRMEELRHHFKIEFAVAEGAKNVMKLLGSGKVTDRKALSEAQARFNESSQKLDLLKYSLEQRLN ELPRNHPKSSVVIEELSLVASPTLSPRQSMLSTQNQYSTLSKPAALTGTLEVRLMGCQDILENVPGRSKA TSVALPGWSPSDNRSSFMSRTSKSKSGSSRNLLKTDDLSNDVCAVLKLDNTVVGQTSWKPISNQSWDQKF TLELDRSRELEISVYWRDWRSLCAVKFLRLEDFLDNQRHGMCLYLEPQGTLFAEVTFFNPVIERRPKLQR QKKIFSKQQGKTFLRAPQMNINIATWGRLVRRAIPTVNHSGTFSPQTPVPATVPVVDARIPDLAPPASDS TVTKLDFDLEPEPPPAPPRASSLGETDESSELRVLDIPGQGSETVFNIENDRNNLRPKSKSEYELSIPDS GRSCWGVGELDDKRAQQRFQFSLQDFRCCAVLGRGHFGKVLLAEYKHTNEMFAIKALKKGDIVARDEVDS LMCEKRIFETVNSVRHPFLVNLFACFQTKEHVCFVMEYAAGGDLMMHIHTDVFSEPRAVFYAACVVLGLQ YLHEHKIVYRDLKLDNLLLDTEGFVKIADFGLCKEGMGYGDRTSTFCGTPEFLAPEVLTETSYTRAVDWW GLGVLIYEMLVGESPFPGDDEEEVFDSIVNDEVRYPRFLSTEAISIMRRLLRRNPERRLGAGEKDAEDVK KHPFFRLTDWSALMDKKVKPPFVPTIRGREDVSNFDDEFTSEAPILTPPREPRILLEEEQEMFHDFDYVA DWC TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_178654 |
ORF Size | 2949 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_178654.4, NP_848769.2 |
RefSeq Size | 6207 bp |
RefSeq ORF | 2952 bp |
Locus ID | 109333 |
UniProt ID | Q8BWW9 |
MW | 112.1 kDa |
Gene Summary | PKC-related serine/threonine-protein kinase and Rho/Rac effector protein that participates in specific signal transduction responses in the cell. Plays a role in the regulation of cell cycle progression, actin cytoskeleton assembly, cell migration, cell adhesion, tumor cell invasion and transcription activation signaling processes. Phosphorylates CTTN in hyaluronan-induced astrocytes and hence decreases CTTN ability to associate with filamentous actin. Phosphorylates HDAC5, therefore lead to impair HDAC5 import. Direct RhoA target required for the regulation of the maturation of primordial junctions into apical junction formation in bronchial epithelial cells. Required for G2/M phases of the cell cycle progression and abscission during cytokinesis in a ECT2-dependent manner. Stimulates FYN kinase activity that is required for establishment of skin cell-cell adhesion during keratinocytes differentiation. Regulates epithelial bladder cells speed and direction of movement during cell migration and tumor cell invasion. Inhibits Akt pro-survival-induced kinase activity. Mediates Rho protein-induced transcriptional activation via the c-fos serum response factor (SRF). Involved in the negative regulation of ciliogenesis (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC222959 | Pkn2 (untagged) - Mouse protein kinase N2 (Pkn2), (10ug) |
CNY 12,640.00 |
|
MG225284 | Pkn2 (tGFP-tagged) - Mouse protein kinase N2 (Pkn2), (10ug) |
CNY 9,500.00 |
|
MR225284L3 | Lenti ORF clone of Pkn2 (Myc-DDK-tagged) - Mouse protein kinase N2 (Pkn2) |
CNY 10,550.00 |
|
MR225284L4 | Lenti ORF clone of Pkn2 (mGFP-tagged) - Mouse protein kinase N2 (Pkn2) |
CNY 10,550.00 |