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Dlc1 (NM_015802) Mouse Tagged ORF Clone
CAT＃: MR225228
- TrueORF®
Dlc1 (Myc-DDK-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 2
ORF Plasmid: tGFP
"NM_015802" in other vectors (4)
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CNY 12,168.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A730069N07Rik; Arhgap7; dlc-1; HP; STARD12 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225228 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTAGAGACGAGCCGGACACCATGATCCTAACACAAATTGAAGCCAAGGAAGCTTGCGACTGGCTGA GAGTGACTGGTTTCCCCCAGTATGCACAGCTGTATGAAGATCTACTGTTCCCTATTGATATTGCGCTGGT CAAGAGAGAACATGACTTCTTGGACAGAGACGCCATCGAGGCTCTCTGCAGGCGCTTAAATACTCTAAAC AAGTGTGCAGTCATGAAGCTGGAGATCAGTCCTCACCGGAAGCGAAGTGAGGATTCGGATGAAGATGAGC CTTGCGCCATAAGCGGCAAGTGGACTTTCCAGAGGGACAGCAAGCGGTGGTCCCGCCTTGAAGAGTTTGA CGTCTTTTCCCCAAAGCAGGATCCAATCCCTGGGTCCCCAGACAACTCTCGTTTGCAAAGCGCCACAAGC CACGAAAGCATGCTGACAGACCTCAGCGAGCACCAGGAGGTGGCCTCTGTCCGAAGCCTCAGCAGCACCA GCAGCAGCGTCCCCACCCACGCAGCCCACAGTGGAGATGCCACTACGCCCCGAACCAATTCCGTCATCAG CGTCTGCTCCTCCGGACACTTTGTAGGCAATGATGACTCTTTTTCCAGCCTGCCGTCTCCCAAGGAACTG TCCAGCTTCAGTTTTAGCATGAAAGGCCACCACGAGAAGAACACCAAGTCGAAGACGCGGAGCCTGCTCA AACGCATGGAGAGCCTGAAGCTCAAGGGCTCCCACCACAGCAAGCACAAGGCGCCTTCCAAGCTGGGGTT GATCATCAGTGCTCCCATTCTGCAGGAGGGTATGGATGAGGCAAAGCTGAAGCAGCTGAACTGTGTGGAG ATCTCAGCCCTCAATGGCAACCACATCAACGTGCCCATGGTACGGAAAAGGAGCGTGTCTAACTCCACCC AGACCAGCAGCAGCAGCAGCCAATCAGAGACCAGCAGCGCGGTCAGCACACCCAGCCCGGTCACCAGGAC CCGGAGCCTCAGCACCTGTAACAAGCGGGTGGGCATGTATCTAGAGGGCTTCGACCCATTCAGTCAGTCC ACCTTGAACAACGTGACGGAGCAGAACTATAAAAACCGTGAGAGCTACCCAGAGGACACGGTGTTCTACA TTCCCGAAGATCACAAGCCCGGCACCTTCCCTAAGGCCCTCTCCCATGGCAGTTTCTGTCCCTCGGGAAA CAGTTCTGTGAACTGGAGGACCGGAAGCTTCCATGGCCCTGGCCATCTCAGCCTACGGAGAGAAAACAGC CATGACAGTCCTAAGGAGCTGAAGAGACGCAATTCTTCCAGCTCTCTGAGCAGCCGCCTGAGCATCTATG ATAACGTACCGGGTTCTATCCTGTACTCCAGCTCGGGAGAACTGGCCGACCTGGAGAATGAGGACATCTT CCCTGAGCTGGATGACATTCTCTACCACGTGAAGGGGATGCAGCGGATAGTCAACCAGTGGTCCGAGAAG TTTTCCGACGAGGGAGACTCGGACTCAGCCCTGGACTCTGTCTCTCCTTGCCCGTCATCTCCAAAACAGA TACACCTGGATGTGGACCATGACCGAAGGACACCCAGTGACCTGGACAGCACAGGCAACTCCCTCAATGA GCCCGAAGAGCCCACTGATATCCCGGAAAGAAGAGACTCAGGGGTGGGGGCTTCCCTGACCAGGTGCAAT AGGCACAGACTGAGGTGGCACAGCTTCCAGAGCTCCCACCGGCCGAGCCTCAACTCCGTGTCACTGCAGA TTAACTGCCAGTCTGTGGCCCAGATGAACCTGCTGCAGAAATACTCGCTCCTGAAATTGACGGCCCTGCT GGAAAAGTACACGCCTTCCAACAAGCACGGCTTCAGCTGGGCTGTGCCCAAGTTCATGAAAAGGATCAAG GTTCCAGACTACAAGGACCGGAGTGTGTTTGGGGTCCCACTGACTGTGAACGTGCAGCGCTCAGGACAGC CCCTGCCCCAGAGCATCCAGCAGGCCATGCGCTACCTCCGTAACCACTGTCTGGACCAGGTCGGGCTCTT CAGGAAGTCAGGTGTCAAATCCCGGATCCAGGCTCTACGCCAGATGAATGAAAGCGCTGAAGATAATGTC AACTATGAAGGCCAGTCTGCTTATGATGTGGCAGACATGTTAAAGCAATATTTTCGAGATCTTCCTGAGC CCCTCATGACGAACAAACTCTCCGAAACCTTCCTGCAGATCTACCAGTATGTTCCCAAGGACCAGCGCCT CCAAGCCATCAAGGCGGCCATTATGCTCCTGCCCGACGAGAACCGGGAGGTTCTGCAGACACTTCTCTAT TTCCTGAGCGATGTCACAGCGGCTGTGAAAGAAAACCAGATGACTCCCACCAACCTGGCTGTGTGCCTAG CTCCGTCCCTCTTCCACCTCAACACCCTGAAGCGAGAGAATTCTTCTCCAAGGGTAATGCAAAGAAAACA GAGTTTGGGCAAACCAGACCAGAAAGACCTGAATGAAAACCTAGCGGCGACTCAAGGGCTGGCCCACATG ATTGCTGAGTGCAAGAAGCTCTTCCAGGTCCCTGAGGAAATGAGCCGGTGCCGTAACTCCTACACTGAAC AAGAGCTGAAGCCCCTTACCCTGGAGGCCTTGGGACACCTGAATAGTGACCAGCCTGCTGACTACAGACA CTTCCTCCAGGACTGTGTGGATGGCCTGTTTAAGGAGGTCAAAGAGAAGTTCAAAGGCTGGGTCAGCTAC CCCACCTCCGAACAGGCTGAGCTGTCCTATAAGAAGGTCAGCGAAGGACCCCCGTTAAGGCTTTGGAGGT CAACTATCGAAGTCCCCGCTGCACCCGAGGAGATCTTAAAGCGCCTTCTGAAGGAGCAACACCTCTGGGA TGTGGACCTGCTGGACTCCAAGGTGATTGAAATCCTGGACAGCCAGACTGAAATCTACCAATACGTCCAA AACAGCATGGCGCCCCACCCTGCGCGGGACTACGTCGTATTGAGGACCTGGAGGACTAACTTACCCAGGG GGGCCTGTGCTCTCTTACTCACCTCTGTGGATCACGACCGGGCCCCCGTGGCGGGGGTGAGGGTTAATGT GCTCCTGTCCAGATATTTGATTGAACCCTGCGGGTCAGGAAAATCCAAGCTTACCTACATGTGCAGAGCT GATTTAAGGGGCCACATGCCGGAGTGGTACTCAAAATCTTTTGGACATTTGTGTGCAGCCGAAGTGGTAA AGATCCGAGACTCCTTCAGTAATCAGAACACTGAAAGCAAGGACACGAGATCTAGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225228 protein sequence
Red=Cloning site Green=Tags(s) MCRDEPDTMILTQIEAKEACDWLRVTGFPQYAQLYEDLLFPIDIALVKREHDFLDRDAIEALCRRLNTLN KCAVMKLEISPHRKRSEDSDEDEPCAISGKWTFQRDSKRWSRLEEFDVFSPKQDPIPGSPDNSRLQSATS HESMLTDLSEHQEVASVRSLSSTSSSVPTHAAHSGDATTPRTNSVISVCSSGHFVGNDDSFSSLPSPKEL SSFSFSMKGHHEKNTKSKTRSLLKRMESLKLKGSHHSKHKAPSKLGLIISAPILQEGMDEAKLKQLNCVE ISALNGNHINVPMVRKRSVSNSTQTSSSSSQSETSSAVSTPSPVTRTRSLSTCNKRVGMYLEGFDPFSQS TLNNVTEQNYKNRESYPEDTVFYIPEDHKPGTFPKALSHGSFCPSGNSSVNWRTGSFHGPGHLSLRRENS HDSPKELKRRNSSSSLSSRLSIYDNVPGSILYSSSGELADLENEDIFPELDDILYHVKGMQRIVNQWSEK FSDEGDSDSALDSVSPCPSSPKQIHLDVDHDRRTPSDLDSTGNSLNEPEEPTDIPERRDSGVGASLTRCN RHRLRWHSFQSSHRPSLNSVSLQINCQSVAQMNLLQKYSLLKLTALLEKYTPSNKHGFSWAVPKFMKRIK VPDYKDRSVFGVPLTVNVQRSGQPLPQSIQQAMRYLRNHCLDQVGLFRKSGVKSRIQALRQMNESAEDNV NYEGQSAYDVADMLKQYFRDLPEPLMTNKLSETFLQIYQYVPKDQRLQAIKAAIMLLPDENREVLQTLLY FLSDVTAAVKENQMTPTNLAVCLAPSLFHLNTLKRENSSPRVMQRKQSLGKPDQKDLNENLAATQGLAHM IAECKKLFQVPEEMSRCRNSYTEQELKPLTLEALGHLNSDQPADYRHFLQDCVDGLFKEVKEKFKGWVSY PTSEQAELSYKKVSEGPPLRLWRSTIEVPAAPEEILKRLLKEQHLWDVDLLDSKVIEILDSQTEIYQYVQ NSMAPHPARDYVVLRTWRTNLPRGACALLLTSVDHDRAPVAGVRVNVLLSRYLIEPCGSGKSKLTYMCRA DLRGHMPEWYSKSFGHLCAAEVVKIRDSFSNQNTESKDTRSR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_015802 |
| ORF Size | 3276 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 6184 bp |
| RefSeq ORF | 3279 bp |
| Locus ID | 50768 |
| UniProt ID | Q9R0Z9 |
| MW | 123.3 kDa |
| Gene Summary | Functions as a GTPase-activating protein for the small GTPases RHOA, RHOB, RHOC and CDC42, terminating their downstream signaling. This induces morphological changes and detachment through cytoskeletal reorganization, playing a critical role in biological processes such as cell migration and proliferation. Also functions in vivo as an activator of the phospholipase PLCD1. Active DLC1 increases cell migration velocity but reduces directionality (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223509 | Dlc1 (untagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 2, (10ug) |
CNY 13,970.00 |
|
| MG225228 | Dlc1 (tGFP-tagged) - Mouse deleted in liver cancer 1 (Dlc1) transcript variant 2, (10ug) |
CNY 12,070.00 |
|
| MR225228L3 | Lenti ORF clone of Dlc1 (Myc-DDK-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 2 |
CNY 12,830.00 |
|
| MR225228L4 | Lenti ORF clone of Dlc1 (mGFP-tagged) - Mouse deleted in liver cancer 1 (Dlc1), transcript variant 2 |
CNY 12,830.00 |
