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Cldn18 (NM_019815) Mouse Tagged ORF Clone
CAT＃: MR225222
- TrueORF®
Cldn18 (Myc-DDK-tagged) - Mouse claudin 18 (Cldn18), transcript variant A1.1
ORF Plasmid: tGFP
"NM_019815" in other vectors (4)
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CNY 2,400.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225222 representing NM_019815
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACCACCACGTGCCAGGTGGTAGGGCTTCTCCTGTCCCTCCTGGGTCTGGCCGGCTGCATAGCCG CCACTGGGATGGACATGTGGAGCACTCAAGACCTGTATGACAACCCAGTCACCGCCGTGTTCCAGTATGA AGGGCTCTGGAGGAGTTGCGTGCAACAGAGCTCGGGGTTCACCGAGTGCCGGCCATACTTCACCATCCTG GGCCTTCCAGCCATGCTGCAAGCTGTACGAGCCCTGATGATCGTGGGCATTGTTCTGGGGGTCATCGGTA TCCTCGTGTCCATCTTCGCCCTGAAGTGCATTCGCATTGGTAGCATGGATGACTCTGCCAAGGCCAAGAT GACTCTGACTTCTGGGATCTTGTTCATCATCTCCGGCATCTGTGCAATCATTGGTGTGTCTGTGTTTGCC AACATGCTGGTGACCAACTTCTGGATGTCCACAGCTAACATGTACAGCGGCATGGGCGGCATGGGTGGCA TGGTGCAGACCGTTCAGACCAGGTACACCTTTGGTGCAGCTCTGTTCGTGGGCTGGGTTGCTGGAGGCCT CACCCTGATTGGGGGAGTGATGATGTGCATCGCCTGCCGTGGCCTGACACCAGATGACAGCAACTTCAAA GCTGTGTCTTACCATGCCTCTGGCCAAAATGTTGCCTACAGGCCTGGAGGCTTTAAGGCCAGCACTGGCT TTGGGTCCAACACCAGAAACAAGAAGATCTACGATGGGGGTGCCCGCACAGAAGACGATGAACAGTCTCA TCCTACCAAGTATGACTATGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225222 representing NM_019815
Red=Cloning site Green=Tags(s) MATTTCQVVGLLLSLLGLAGCIAATGMDMWSTQDLYDNPVTAVFQYEGLWRSCVQQSSGFTECRPYFTIL GLPAMLQAVRALMIVGIVLGVIGILVSIFALKCIRIGSMDDSAKAKMTLTSGILFIISGICAIIGVSVFA NMLVTNFWMSTANMYSGMGGMGGMVQTVQTRYTFGAALFVGWVAGGLTLIGGVMMCIACRGLTPDDSNFK AVSYHASGQNVAYRPGGFKASTGFGSNTRNKKIYDGGARTEDDEQSHPTKYDYV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019815 |
| ORF Size | 792 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019815.3, NP_062789.1 |
| RefSeq Size | 2842 bp |
| RefSeq ORF | 795 bp |
| Locus ID | 56492 |
| UniProt ID | P56857 |
| MW | 28.6 kDa |
| Gene Summary | This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets, and also play critical roles in maintaining cell polarity and signal transductions. This gene is a downstream target gene regulated by the T/EBP/NKX2.1 homeodomain transcription factor. Four alternatively spliced transcript variants resulted from alternative promoters and alternative splicing have been identified, which encode two lung-specific isoforms and two stomach-specific isoforms respectively. This gene is also expressed in colons, inner ear and skin, and its expression is increased in both experimental colitis and ulcerative colitis. [provided by RefSeq, Aug 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC210060 | Cldn18 (untagged) - Mouse claudin 18 (Cldn18), transcript variant A1.1, (10ug) |
CNY 3,600.00 |
|
| MG225222 | Cldn18 (tGFP-tagged) - Mouse claudin 18 (Cldn18) transcript variant A1.1, (10ug) |
CNY 4,370.00 |
|
| MR225222L3 | Lenti ORF clone of Cldn18 (Myc-DDK-tagged) - Mouse claudin 18 (Cldn18), transcript variant A1.1 |
CNY 4,800.00 |
|
| MR225222L4 | Lenti ORF clone of Cldn18 (mGFP-tagged) - Mouse claudin 18 (Cldn18), transcript variant A1.1 |
CNY 4,800.00 |
