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Smarcc1 (NM_009211) Mouse Tagged ORF Clone
CAT＃: MR225193
- TrueORF®
Smarcc1 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 (Smarcc1)
ORF Plasmid: tGFP
"NM_009211" in other vectors (4)
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CNY 11,020.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI115498; BAF155; msp3; Rsc8; SRG3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225193 representing NM_009211
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCGACAGCGGGTGGCGGTCCGGGAGCAGCAGCAGGCGCCGTGGGTGCAGGGGGTGCGGCGGCGG CCTCCGGGCTGGCCGTGTACCGGAGGAAGGACGGGGGCCCGGCCAGCAAGTTTTGGGAGAGCCCGGACAC GGTGTCCCAGCTAGATTCGGTGCGAGTCTGGCTGGGCAAGCACTACAAGAAGTATGTTCATGCAGATGCT CCTACCAATAAAACACTAGCTGGACTGGTGGTGCAGCTTCTACAGTTCCAAGAAGATGCCTTTGGGAAGC ATGTCACCAACCCAGCTTTCACCAAACTACCTGCAAAATGTTTCATGGATTTCAAAGCTGGAGGCACCTT GTGTCACATTCTTGGGGCAGCTTACAAGTACAAAAATGAACAGGGCTGGCGGAGATTTGATCTTCAGAAC CCATCCCGAATGGATCGTAACGTTGAAATGTTCATGAACATTGAGAAAACATTGGTACAGAACAACTGTC TGACTAGACCAAACATCTACCTCATTCCAGACATTGATTTGAAGTTGGCTAACAAGTTGAAAGATATCAT CAAACGGCATCAGGGGACATTTACTGATGAGAAGTCAAAAGCTTCCCACCATATTTATCCATATCCTTCC TCACAAGAGGATGAGGAGTGGCTGAGACCAGTGATGAGGAGAGACAAGCAGGTGCTGGTGCACTGGGGTT TCTACCCAGACAGCTATGACACTTGGGTCCACAGTAATGATGTTGATGCTGAAATTGAAGATGCACCAAT CCCAGAAAAGCCCTGGAAGGTTCATGTAAAATGGATTTTGGACACTGACGTTTTCAATGAATGGATGAAT GAAGAGGATTATGAAGTGGATGAGAACAGAAAGCCAGTGAGCTTTCGTCAACGAATTTCAACAAAGAATG AAGAGCCAGTCAGAAGTCCAGAAAGGAGAGACAGAAAAGCCTCTGCCAACTCTAGGAAGAGGAAACCTTC CCCTTCTCCTCCTCCTCCCACAGCCACAGAGTCCCGCAAGAAGAGCGGGAAGAAAGGACAAGCTAGCCTT TATGGGAAACGTAGAAGTCAGAAGGAAGAAGATGAGCAAGAAGATCTTACCAAGGACATGGAAGACCCCA CACCTGTACCTAACATAGAGGAAGTGGTTCTCCCTAAGAATGTAAACCCAAAGAAGGACAGTGAAAACAC ACCCGTTAAAGGAGGCACGGTGGCAGATCTAGATGAGCAGGATGAAGAAGCAGTTACAACAGGAGGAAAG GAAGATGAAGATCCCAGCAAAGGTGATCCAAGTCGCTCAGTTGACCCAGGTGAAGACAACGTGACAGAAC AGACCAATCACATCATTATTCCCAGCTACGCATCCTGGTTTGATTATAATTGTATTCATGTCATTGAACG GCGTGCGCTTCCTGAGTTCTTTAATGGAAAAAACAAATCCAAGACCCCTGAAATATACTTGGCATATCGA AATTTTATGATTGACACATACCGTCTAAACCCTCAAGAATATTTAACCAGCACTGCTTGCCGGCGAAACC TGACTGGAGATGTGTGTGCTGTGATGAGGGTTCATGCCTTCTTAGAGCAGTGGGGTCTTGTTAACTACCA AGTTGACCCAGAGAGTCGACCCATGGCAATGGGACCTCCTCCCACTCCTCACTTCAATGTGTTAGCTGAC ACACCCTCTGGGCTTGTGCCCCTGCATCTTCGATCACCTCAGGTCCCTGCCGCTCAACAGATGTTAAATT TTCCTGAGAAGAACAAGGAAAAACCAATTGATTTGCAGAACTTTGGTCTTCGAACTGACATTTACTCCAA GAAAACACTGGCAAAGAGTAAAGGTGCTAGTGCTGGAAGGGAGTGGACAGAACAGGAGACCCTTCTTCTC CTAGAGGCTCTGGAGATGTACAAGGACGATTGGAATAAAGTGTCAGAACATGTTGGAAGCCGTACTCAGG ACGAATGCATCCTCCACTTTCTGAGGCTTCCCATTGAGGACCCTTACCTTGAAAATTCAGATGCTTCTCT TGGGCCACTGGCTTACCAGCCTGTCCCTTTCAGCCAGTCGGGAAACCCGGTGATGAGCACTGTTGCCTTT TTAGCATCTGTCGTTGACCCCCGTGTAGCATCTGCTGCAGCAAAAGCAGCGTTGGAGGAGTTTTCTCGTG TCCGAGAAGAAGTACCCCTGGAATTGGTTGAAGCACATGTCAAGAAAGTACAGGAAGCTGCAAGAGCCTC TGGGAAGGTGGACCCCACCTATGGCTTGGAGAGCAGCTGTATTGCTGGCACAGGGCCTGACGAGCCAGAG AAGCTTGAAGGATCTGAAGAAGAGAAGATGGAAACAGATCCTGATGGTCAGCAGCCTGAAAAGGCAGAAA ACAAAGTGGAAAATGAATCGGATGAAGGTGATAAAATACAAGATCGAGAGAATGAAAAAAACACTGAGAA GGAACAAGATAGTGACGTCAGTGAGGATGTCAAGCCAGAAGAAAAGGAGAATGAAGAGAACAAAGAGCTC ACTGATACATGTAAAGAAAGAGAAAGCGATGCCGGGAAGAAGAAAGTGGAACACGAGATTTCGGAAGGAA ACGTTGCCACAGCCGCAGCAGCTGCTCTGGCCTCAGCTGCTACTAAAGCCAAGCACCTGGCGGCTGTTGA AGAAAGAAAAATCAAGTCCTTGGTAGCTCTCTTGGTTGAAACACAAATGAAGAAACTAGAGATCAAACTT CGACATTTTGAAGAGCTGGAGACTATAATGGACAGAGAGAAAGAGGCTCTAGAACAACAGAGACAGCAGT TGCTTACTGAGCGTCAGAACTTCCACATGGAACAGTTGAAATATGCTGAACTACGTGCCCGGCAGCAAAT GGAGCAGCAGCAGCAGCATGGCCAGACACCTCAGCAGGCGCACCAGCACACGGGAGGGCCGGGGATGGCC CCACTTGGAGCCACAGGCCACCCTGGCATGATGCCGCATCAGCAGCCCCCTCCCTACCCACTGATGCACC ATCAGATGCCGCCACCCCATCCTCCCCAACCAGGTCAAATACCAGGCCCTGGCTCCATGATGCCTGGCCA GCCCATGCCAGGTCGCATGATCCCCGCTGTGGCAGCCAACATTCACCCTACTGGGAGTGGCCCTACCCCT CCTGGTATGCCTCCAATGCCCGGAAACATCTTAGGACCCCGGGTACCCCTCACAGCACCAAACGGCATGT ATCCTCCTCCACCACAGCAGCAGCAGCCGCCTCCTCCTGCAGATGGGGTCCCTCCACCTCCTGCTCCAGG CCCACCCGCCTCGGCCACTCCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225193 representing NM_009211
Red=Cloning site Green=Tags(s) MAATAGGGPGAAAGAVGAGGAAAASGLAVYRRKDGGPASKFWESPDTVSQLDSVRVWLGKHYKKYVHADA PTNKTLAGLVVQLLQFQEDAFGKHVTNPAFTKLPAKCFMDFKAGGTLCHILGAAYKYKNEQGWRRFDLQN PSRMDRNVEMFMNIEKTLVQNNCLTRPNIYLIPDIDLKLANKLKDIIKRHQGTFTDEKSKASHHIYPYPS SQEDEEWLRPVMRRDKQVLVHWGFYPDSYDTWVHSNDVDAEIEDAPIPEKPWKVHVKWILDTDVFNEWMN EEDYEVDENRKPVSFRQRISTKNEEPVRSPERRDRKASANSRKRKPSPSPPPPTATESRKKSGKKGQASL YGKRRSQKEEDEQEDLTKDMEDPTPVPNIEEVVLPKNVNPKKDSENTPVKGGTVADLDEQDEEAVTTGGK EDEDPSKGDPSRSVDPGEDNVTEQTNHIIIPSYASWFDYNCIHVIERRALPEFFNGKNKSKTPEIYLAYR NFMIDTYRLNPQEYLTSTACRRNLTGDVCAVMRVHAFLEQWGLVNYQVDPESRPMAMGPPPTPHFNVLAD TPSGLVPLHLRSPQVPAAQQMLNFPEKNKEKPIDLQNFGLRTDIYSKKTLAKSKGASAGREWTEQETLLL LEALEMYKDDWNKVSEHVGSRTQDECILHFLRLPIEDPYLENSDASLGPLAYQPVPFSQSGNPVMSTVAF LASVVDPRVASAAAKAALEEFSRVREEVPLELVEAHVKKVQEAARASGKVDPTYGLESSCIAGTGPDEPE KLEGSEEEKMETDPDGQQPEKAENKVENESDEGDKIQDRENEKNTEKEQDSDVSEDVKPEEKENEENKEL TDTCKERESDAGKKKVEHEISEGNVATAAAAALASAATKAKHLAAVEERKIKSLVALLVETQMKKLEIKL RHFEELETIMDREKEALEQQRQQLLTERQNFHMEQLKYAELRARQQMEQQQQHGQTPQQAHQHTGGPGMA PLGATGHPGMMPHQQPPPYPLMHHQMPPPHPPQPGQIPGPGSMMPGQPMPGRMIPAVAANIHPTGSGPTP PGMPPMPGNILGPRVPLTAPNGMYPPPPQQQQPPPPADGVPPPPAPGPPASATP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009211 |
| ORF Size | 3312 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009211.2, NP_033237.2 |
| RefSeq Size | 5684 bp |
| RefSeq ORF | 3315 bp |
| Locus ID | 20588 |
| UniProt ID | P97496 |
| MW | 123.3 kDa |
| Gene Summary | Involved in transcriptional activation and repression of select genes by chromatin remodeling (alteration of DNA-nucleosome topology). Component of SWI/SNF chromatin remodeling complexes that carry out key enzymatic activities, changing chromatin structure by altering DNA-histone contacts within a nucleosome in an ATP-dependent manner. May stimulate the ATPase activity of the catalytic subunit of the complex. Belongs to the neural progenitors-specific chromatin remodeling complex (npBAF complex) and the neuron-specific chromatin remodeling complex (nBAF complex). During neural development a switch from a stem/progenitor to a postmitotic chromatin remodeling mechanism occurs as neurons exit the cell cycle and become committed to their adult state. The transition from proliferating neural stem/progenitor cells to postmitotic neurons requires a switch in subunit composition of the npBAF and nBAF complexes. As neural progenitors exit mitosis and differentiate into neurons, npBAF complexes which contain ACTL6A/BAF53A and PHF10/BAF45A, are exchanged for homologous alternative ACTL6B/BAF53B and DPF1/BAF45B or DPF3/BAF45C subunits in neuron-specific complexes (nBAF). The npBAF complex is essential for the self-renewal/proliferative capacity of the multipotent neural stem cells. The nBAF complex along with CREST plays a role regulating the activity of genes essential for dendrite growth.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223589 | Smarcc1 (untagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 (Smarcc1), (10ug) |
CNY 14,160.00 |
|
| MG225193 | Smarcc1 (tGFP-tagged) - Mouse SWI/SNF related matrix associated actin dependent regulator of chromatin subfamily c member 1 (Smarcc1), (10ug) |
CNY 12,160.00 |
|
| MR225193L3 | Lenti ORF clone of Smarcc1 (Myc-DDK-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 (Smarcc1) |
CNY 12,920.00 |
|
| MR225193L4 | Lenti ORF clone of Smarcc1 (mGFP-tagged) - Mouse SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily c, member 1 (Smarcc1) |
CNY 12,920.00 |
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