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Gnptab (NM_001004164) Mouse Tagged ORF Clone
CAT＃: MR225190
- TrueORF®
Gnptab (Myc-DDK-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (Gnptab)
ORF Plasmid: tGFP
"NM_001004164" in other vectors (4)
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CNY 12,896.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | EG432486; mKIAA1208 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225190 representing NM_001004164
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTTAAGCTCCTGCAGAGACAGACCTATACCTGCCTATCCCACAGGTATGGGCTCTACGTCTGCT TCGTGGGCGTCGTTGTCACCATCGTCTCGGCTTTCCAGTTCGGAGAGGTGGTTCTGGAATGGAGCCGAGA TCAGTACCATGTTTTGTTTGATTCCTACAGAGACAACATTGCTGGGAAATCCTTTCAGAATCGGCTCTGT CTGCCCATGCCAATCGACGTGGTTTACACCTGGGTGAATGGCACTGACCTTGAACTGCTAAAGGAGCTAC AGCAGGTCCGAGAGCACATGGAGGAAGAGCAGAGAGCCATGCGGGAAACCCTCGGGAAGAACACAACCGA ACCGACAAAGAAGAGTGAGAAGCAGCTGGAATGTCTGCTGACGCACTGCATTAAGGTGCCCATGCTTGTT CTGGACCCGCCCCTGCCAGCCAACTGCACCCTGAAGGATCTGCCAACCCTTTATCCATCTTTCCACGCTG CCAGCGACATGTTCAATGTTGCGAAACCAAAAAATCCGTCTACTAATGTCTCCGTTGTCGTTTTTGACAC TACTAAGGATGTTGAAGACGCCCATGCTGGACCGTTTAAGGGAGGCAGCAAACAGATGGTTTGGAGAGCC TACTTGACAACAGACAAAGAAGCCCCTGGCTTAGTGCTGATGCAAGGCTTGGCGTTCCTGAGTGGATTCC CACCGACCTTCAAGGAGACGAGTCAACTGAAGACAAAGCTGCCAGAAAAGCTTTCCTCTAAAATAAAGCT GTTGCGGCTGTACTCGGAGGCCAGTGTCGCTCTTCTGAAATTGAATAATCCCAAGGGTTTCCAAGAGCTG AACAAGCAGACCAAGAAGAACATGACCATCGATGGGAAGGAACTGACCATCAGCCCTGCGTATCTGCTGT GGGACCTGAGTGCCATCAGCCAGTCCAAGCAGGATGAGGACGTGTCTGCCAGCCGCTTTGAGGATAATGA AGAGCTGAGGTACTCGCTGCGATCTATCGAGAGACACGCGCCATGGGTACGGAATATTTTCATTGTCACC AACGGGCAGATTCCATCCTGGCTGAACCTTGACAACCCTCGAGTGACCATAGTGACCCACCAGGACATTT TCCAAAATCTGAGCCACTTGCCTACTTTCAGTTCCCCTGCTATTGAAAGTCACATTCACCGCATCGAAGG GCTGTCCCAGAAGTTTATTTATCTAAATGACGATGTCATGTTCGGTAAGGACGTCTGGCCGGACGATTTT TACAGCCACTCCAAAGGTCAAAAGGTTTATTTGACATGGCCTGTGCCAAACTGTGCAGAGGGCTGCCCGG GCTCCTGGATTAAAGACGGCTATTGTGATAAGGCCTGTAATAACTCAGCCTGTGACTGGGATGGCGGAGA CTGCTCTGGTAATACTGCAGGGAACCGGTTTGTTGCAGGAGGTGGGGGTACCGGGAATATTGGAGCTGGA CAGCACTGGCAGTTTGGTGGAGGAATAAACACCATCTCTTACTGTAACCAAGGATGTGCAAACTCCTGGC TGGCTGACAAGTTCTGTGACCAAGCCTGTAACGTCTTATCCTGCGGGTTTGATGCTGGTGACTGTGGACA AGATCATTTTCATGAATTGTATAAAGTAACACTTCTCCCAAACCAGACTCACTATGTTGTCCCCAAAGGT GAATACCTGTCTTATTTCAGCTTTGCAAACATAGCCAGAAGAGGAGTTGAAGGGACCTACAGCGACAACC CCATCATCCGCCACGCGTCCATTGCAAACAAGTGGAAAACCATACACCTGATAATGCACAGTGGGATGAA CGCAACCACGATCTATTTTAACCTCACTCTTCAAAACGCCAACGACGAAGAGTTCAAGATCCAGATAGCA GTAGAGGTGGACACGAGGGAGGCGCCCAAACTGAATTCTACAACCCAGAAGGCCTATGAAAGTTTGGTTA GCCCAGTGACACCTCTTCCTCAGGCTGACGTCCCTTTTGAAGATGTCCCCAAAGAGAAACGCTTCCCCAA GATCAGGAGACATGATGTAAATGCAACAGGGAGATTCCAAGAGGAGGTGAAAATCCCCCGGGTAAATATT TCACTCCTTCCCAAAGAGGCCCAGGTGAGGCTGAGCAACTTGGATTTGCAACTAGAACGTGGAGACATCA CTCTGAAAGGATATAACTTGTCCAAGTCAGCCCTGCTAAGGTCTTTCCTGGGGAATTCACTAGATACTAA AATAAAACCTCAAGCTAGGACCGATGAAACAAAAGGCAACCTGGAGGTCCCACAGGAAAACCCTTCTCAC AGACGTCCACATGGCTTTGCTGGTGAACACAGATCAGAGAGATGGACTGCCCCAGCAGAGACAGTGACCG TGAAAGGCCGTGACCACGCTTTGAATCCACCCCCGGTGTTGGAGACCAATGCAAGATTGGCCCAGCCTAC ACTAGGCGTGACTGTGTCCAAAGAGAACCTTTCACCGCTGATCGTTCCCCCAGAAAGCCACTTGCCAAAA GAAGAGGAGAGTGACAGGGCAGAAGGCAATGCTGTACCTGTAAAGGAGTTAGTGCCTGGCAGACGGTTGC AGCAGAATTATCCAGGCTTTTTGCCCTGGGAGAAAAAAAAGTATTTCCAAGACCTTCTTGATGAGGAAGA GTCATTGAAGACCCAGTTGGCGTACTTTACAGACAGCAAACATACCGGGAGGCAACTAAAAGATACATTT GCAGACTCCCTCCGATACGTCAATAAAATTCTCAACAGCAAGTTTGGATTCACATCCAGGAAAGTCCCTG CACACATGCCGCACATGATTGACAGGATCGTTATGCAAGAACTCCAAGATATGTTCCCTGAAGAATTTGA CAAGACTTCATTTCACAAGGTGCGTCACTCTGAGGACATGCAGTTTGCCTTCTCCTACTTTTATTACCTC ATGAGTGCAGTTCAGCCCCTCAATATTTCCCAAGTCTTTCATGAAGTAGACACAGACCAATCTGGTGTCT TGTCTGATAGGGAAATCCGAACACTGGCCACGAGAATTCACGACCTACCTTTAAGCTTGCAGGATTTGAC AGGTTTGGAACACATGTTAATAAATTGCTCAAAAATGCTCCCCGCTAATATCACTCAACTCAACAACATC CCACCGACTCAGGAAGCATACTACGACCCCAACCTGCCTCCGGTCACTAAGAGTCTTGTCACCAACTGTA AGCCAGTAACTGACAAGATCCACAAAGCCTATAAAGACAAGAACAAATACAGGTTTGAAATCATGGGAGA GGAAGAAATCGCTTTCAAGATGATACGAACCAATGTTTCTCATGTGGTTGGCCAGTTGGATGACATCAGA AAAAACCCCAGGAAGTTCGTTTGTCTGAATGACAACATTGACCACAACCATAAAGATGCCCGGACAGTGA AGGCTGTCCTCAGGGACTTCTATGAGTCCATGTTTCCCATACCTTCCCAGTTTGAGCTGCCAAGAGAGTA TCGGAACCGCTTTCTGCACATGCATGAGCTCCAAGAATGGCGGGCATATCGAGACAAGCTGAAGTTTTGG ACCCACTGCGTACTAGCAACGTTGATTATATTTACTATATTCTCATTTTTTGCTGAACAGATAATTGCTC TGAAGCGAAAGATATTTCCCAGGAGGAGGATACACAAAGAAGCTAGTCCAGACCGAATCAGGGTG AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR225190 representing NM_001004164
Red=Cloning site Green=Tags(s) MLLKLLQRQTYTCLSHRYGLYVCFVGVVVTIVSAFQFGEVVLEWSRDQYHVLFDSYRDNIAGKSFQNRLC LPMPIDVVYTWVNGTDLELLKELQQVREHMEEEQRAMRETLGKNTTEPTKKSEKQLECLLTHCIKVPMLV LDPPLPANCTLKDLPTLYPSFHAASDMFNVAKPKNPSTNVSVVVFDTTKDVEDAHAGPFKGGSKQMVWRA YLTTDKEAPGLVLMQGLAFLSGFPPTFKETSQLKTKLPEKLSSKIKLLRLYSEASVALLKLNNPKGFQEL NKQTKKNMTIDGKELTISPAYLLWDLSAISQSKQDEDVSASRFEDNEELRYSLRSIERHAPWVRNIFIVT NGQIPSWLNLDNPRVTIVTHQDIFQNLSHLPTFSSPAIESHIHRIEGLSQKFIYLNDDVMFGKDVWPDDF YSHSKGQKVYLTWPVPNCAEGCPGSWIKDGYCDKACNNSACDWDGGDCSGNTAGNRFVAGGGGTGNIGAG QHWQFGGGINTISYCNQGCANSWLADKFCDQACNVLSCGFDAGDCGQDHFHELYKVTLLPNQTHYVVPKG EYLSYFSFANIARRGVEGTYSDNPIIRHASIANKWKTIHLIMHSGMNATTIYFNLTLQNANDEEFKIQIA VEVDTREAPKLNSTTQKAYESLVSPVTPLPQADVPFEDVPKEKRFPKIRRHDVNATGRFQEEVKIPRVNI SLLPKEAQVRLSNLDLQLERGDITLKGYNLSKSALLRSFLGNSLDTKIKPQARTDETKGNLEVPQENPSH RRPHGFAGEHRSERWTAPAETVTVKGRDHALNPPPVLETNARLAQPTLGVTVSKENLSPLIVPPESHLPK EEESDRAEGNAVPVKELVPGRRLQQNYPGFLPWEKKKYFQDLLDEEESLKTQLAYFTDSKHTGRQLKDTF ADSLRYVNKILNSKFGFTSRKVPAHMPHMIDRIVMQELQDMFPEEFDKTSFHKVRHSEDMQFAFSYFYYL MSAVQPLNISQVFHEVDTDQSGVLSDREIRTLATRIHDLPLSLQDLTGLEHMLINCSKMLPANITQLNNI PPTQEAYYDPNLPPVTKSLVTNCKPVTDKIHKAYKDKNKYRFEIMGEEEIAFKMIRTNVSHVVGQLDDIR KNPRKFVCLNDNIDHNHKDARTVKAVLRDFYESMFPIPSQFELPREYRNRFLHMHELQEWRAYRDKLKFW THCVLATLIIFTIFSFFAEQIIALKRKIFPRRRIHKEASPDRIRV SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001004164 |
| ORF Size | 3705 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001004164.3 |
| RefSeq Size | 5204 bp |
| RefSeq ORF | 3708 bp |
| Locus ID | 432486 |
| UniProt ID | Q69ZN6 |
| MW | 141.4 kDa |
| Gene Summary | Catalyzes the formation of mannose 6-phosphate (M6P) markers on high mannose type oligosaccharides in the Golgi apparatus. M6P residues are required to bind to the M6P receptors (MPR), which mediate the vesicular transport of lysosomal enzymes to the endosomal/prelysosomal compartment.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224020 | Gnptab (untagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (Gnptab), (10ug) |
CNY 15,770.00 |
|
| MG225190 | Gnptab (tGFP-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase alpha and beta subunits (Gnptab), (10ug) |
CNY 14,496.00 |
|
| MR225190L3 | Lenti ORF clone of Gnptab (Myc-DDK-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (Gnptab) |
CNY 12,730.00 |
|
| MR225190L4 | Lenti ORF clone of Gnptab (mGFP-tagged) - Mouse N-acetylglucosamine-1-phosphate transferase, alpha and beta subunits (Gnptab) |
CNY 12,730.00 |
