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Ube3a (NM_001033962) Mouse Tagged ORF Clone
CAT＃: MR225048
- TrueORF®
Ube3a (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 3
ORF Plasmid: tGFP
"NM_001033962" in other vectors (4)
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CNY 8,460.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4732496B02; 5830462N02Rik; A130086L21Rik; Hpve6a; mKIAA4216 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR225048 representing NM_001033962
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGAGCAGCTGCAAAGCATCTAATAGAACGCTACTACCATCAGTTAACTGAGGGCTGTGGAAATG AGGCCTGCACGAATGAGTTTTGTGCTTCCTGTCCAACTTTTCTTCGTATGGATAACAATGCAGCAGCTAT TAAAGCCCTTGAGCTTTATAAAATTAATGCAAAACTCTGTGATCCTCATCCCTCCAAGAAAGGAGCAAGC TCAGCTTACCTTGAGAACTCAAAAGGTGCATCTAACAACTCAGAGATAAAAATGAACAAGAAGGAAGGAA AAGATTTTAAAGATGTGATTTACCTAACTGAAGAGAAAGTATATGAAATTTATGAATTTTGTAGAGAGAG TGAGGATTATTCCCCTTTAATTCGTGTAATTGGAAGAATATTTTCTAGTGCTGAGGCACTGGTTCTGAGC TTTCGGAAAGTCAAACAGCACACAAAGGAGGAATTGAAATCTCTTCAAGAAAAGGATGAAGACAAGGATG AAGATGAAAAGGAAAAAGCTGCATGTTCTGCTGCTGCTATGGAAGAAGACTCAGAAGCATCTTCTTCAAG GATGGGTGATAGTTCACAGGGAGACAACAATGTACAAAAATTAGGTCCTGATGATGTGACTGTGGATATT GATGCTATTAGAAGGGTCTACAGCAGTTTGCTCGCTAATGAAAAATTAGAAACTGCCTTCCTGAATGCAC TTGTATATCTGTCACCTAACGTGGAATGTGATTTGACATATCATAATGTGTATACTCGAGATCCTAATTA TCTCAATTTGTTCATTATTGTAATGGAGAATAGTAATCTCCACAGTCCTGAATATCTGGAAATGGCGTTG CCATTATTTTGCAAAGCTATGTGTAAGCTACCCCTTGAAGCTCAAGGAAAACTGATTAGGCTGTGGTCTA AATACAGTGCTGACCAGATTCGGAGAATGATGGAAACATTTCAGCAACTTATTACCTACAAAGTCATAAG CAATGAATTTAATAGCCGAAATCTAGTGAATGATGATGATGCCATTGTTGCTGCTTCAAAGTGTTTGAAA ATGGTTTACTATGCAAATGTAGTGGGAGGGGATGTGGACACAAATCATAATGAGGAAGATGATGAAGAAC CCATACCTGAGTCCAGCGAATTAACACTTCAGGAGCTTCTGGGAGATGAAAGAAGAAATAAGAAAGGTCC TCGAGTGGATCCACTAGAAACCGAACTTGGCGTTAAAACTCTAGACTGTCGAAAACCACTTATCTCCTTT GAAGAATTCATTAATGAACCACTGAATGATGTTCTAGAAATGGACAAAGATTATACCTTTTTCAAAGTTG AAACAGAGAACAAATTCTCTTTTATGACATGTCCCTTTATATTGAATGCTGTCACAAAGAATCTGGGATT ATATTATGACAATAGAATTCGCATGTACAGTGAAAGAAGAATCACTGTTCTTTACAGCCTAGTTCAAGGA CAGCAGTTGAATCCGTATTTGAGACTCAAAGTCAGACGTGACCATATTATAGATGATGCACTGGTCCGGC TAGAGATGATTGCTATGGAAAATCCTGCAGACTTGAAGAAGCAGTTGTATGTGGAATTTGAAGGAGAACA AGGAGTAGATGAGGGAGGCGTTTCCAAAGAGTTTTTTCAGTTGGTTGTGGAGGAAATTTTTAATCCAGAT ATTGGTATGTTCACATATGATGAAGCTACGAAATTATTTTGGTTTAATCCATCTTCTTTTGAAACTGAGG GTCAGTTTACTCTGATTGGCATAGTCCTGGGTCTGGCTATTTACAATAATTGTATACTGGATGTCCATTT TCCCATGGTTGTATACAGGAAGCTAATGGGGAAAAAAGGAACCTTTCGTGACTTGGGAGACTCTCACCCA GTTTTATATCAGAGTTTAAAGGATTTATTGGAATATGAAGGGAGTGTGGAAGATGATATGATGATCACTT TCCAGATATCACAGACAGATCTTTTTGGTAACCCAATGATGTATGATCTAAAAGAAAATGGTGATAAAAT TCCAATTACAAATGAAAACAGGAAGGAATTTGTCAATCTCTATTCAGACTACATTCTCAATAAATCTGTA GAAAAACAATTCAAGGCATTTCGCAGAGGTTTTCATATGGTGACTAATGAATCGCCCTTAAAATACTTAT TCAGACCAGAAGAAATTGAATTGCTTATATGTGGAAGCCGGAATCTAGATTTCCAGGCACTAGAAGAAAC TACAGAGTATGACGGTGGCTATACGAGGGAATCTGTTGTGATTAGGGAGTTCTGGGAAATTGTTCATTCG TTTACAGATGAACAGAAAAGACTCTTTCTGCAGTTTACAACAGGCACAGACAGAGCACCTGTTGGAGGAC TAGGAAAATTGAAGATGATTATAGCCAAAAATGGCCCAGACACAGAAAGGTTACCTACATCTCATACTTG CTTTAATGTCCTTTTACTTCCGGAATATTCAAGCAAAGAAAAACTTAAAGAGAGATTGTTGAAGGCCATC ACATATGCCAAAGGATTTGGCATGCTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR225048 representing NM_001033962
Red=Cloning site Green=Tags(s) MKRAAAKHLIERYYHQLTEGCGNEACTNEFCASCPTFLRMDNNAAAIKALELYKINAKLCDPHPSKKGAS SAYLENSKGASNNSEIKMNKKEGKDFKDVIYLTEEKVYEIYEFCRESEDYSPLIRVIGRIFSSAEALVLS FRKVKQHTKEELKSLQEKDEDKDEDEKEKAACSAAAMEEDSEASSSRMGDSSQGDNNVQKLGPDDVTVDI DAIRRVYSSLLANEKLETAFLNALVYLSPNVECDLTYHNVYTRDPNYLNLFIIVMENSNLHSPEYLEMAL PLFCKAMCKLPLEAQGKLIRLWSKYSADQIRRMMETFQQLITYKVISNEFNSRNLVNDDDAIVAASKCLK MVYYANVVGGDVDTNHNEEDDEEPIPESSELTLQELLGDERRNKKGPRVDPLETELGVKTLDCRKPLISF EEFINEPLNDVLEMDKDYTFFKVETENKFSFMTCPFILNAVTKNLGLYYDNRIRMYSERRITVLYSLVQG QQLNPYLRLKVRRDHIIDDALVRLEMIAMENPADLKKQLYVEFEGEQGVDEGGVSKEFFQLVVEEIFNPD IGMFTYDEATKLFWFNPSSFETEGQFTLIGIVLGLAIYNNCILDVHFPMVVYRKLMGKKGTFRDLGDSHP VLYQSLKDLLEYEGSVEDDMMITFQISQTDLFGNPMMYDLKENGDKIPITNENRKEFVNLYSDYILNKSV EKQFKAFRRGFHMVTNESPLKYLFRPEEIELLICGSRNLDFQALEETTEYDGGYTRESVVIREFWEIVHS FTDEQKRLFLQFTTGTDRAPVGGLGKLKMIIAKNGPDTERLPTSHTCFNVLLLPEYSSKEKLKERLLKAI TYAKGFGML TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033962 |
| ORF Size | 2547 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033962.2 |
| RefSeq Size | 4910 bp |
| RefSeq ORF | 2550 bp |
| Locus ID | 22215 |
| MW | 98 kDa |
| Gene Summary | E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and transfers it to its substrates. Several substrates have been identified including the ARNTL/BMAL1, ARC, RAD23A and RAD23B, MCM7 (which is involved in DNA replication), annexin A1, the PML tumor suppressor, and the cell cycle regulator CDKN1B (PubMed:20211139, PubMed:24728990). Additionally, may function as a cellular quality control ubiquitin ligase by helping the degradation of the cytoplasmic misfolded proteins. Finally, UBE3A also promotes its own degradation in vivo (By similarity). Plays an important role in the regulation of the circadian clock: involved in the ubiquitination of the core clock component ARNTL/BMAL1, leading to its proteasomal degradation (PubMed:24728990). Acts as a regulator of synaptic development by mediating ubiquitination and degradation of ARC (PubMed:20211139). Synergizes with WBP2 in enhancing PGR activity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222043 | Ube3a (untagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 3, (10ug) |
CNY 10,830.00 |
|
| MG225048 | Ube3a (tGFP-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a) transcript variant 3, (10ug) |
CNY 9,310.00 |
|
| MR225048L3 | Lenti ORF clone of Ube3a (Myc-DDK-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 3 |
CNY 10,360.00 |
|
| MR225048L4 | Lenti ORF clone of Ube3a (mGFP-tagged) - Mouse ubiquitin protein ligase E3A (Ube3a), transcript variant 3 |
CNY 10,360.00 |
