Sim1 (NM_011376) Mouse Tagged ORF Clone

CAT＃: MR224997

TrueORF^®

Sim1 (Myc-DDK-tagged) - Mouse single-minded homolog 1 (Drosophila) (Sim1)

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_011376" in other vectors (4)

Need custom modification / cloning service?
Get a free quote

Mouse Protein / Lysate Request

CNY 5,600.00

CNY 5,800.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	bHLHe14; mSIM1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR224997 representing NM_011376 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAAGAAAAGTCTAAAAATGCTGCCCGAACGAGGAGGGAGAAAGAAAACAGTGAATTTTATGAACTGG CTAAATTACTGCCTTTACCCTCAGCCATCACCTCCCAACTGGACAAAGCATCCATCATCAGACTCACGAC CAGCTATCTCAAAATGAGAGTGGTCTTTCCAGAAGGGCTTGGCGAGGCGTGGGGCCACACCAGTCGAACC AGCCCCTTGGACAACGTTGGTCGCGAACTCGGCTCTCATCTACTCCAGACTCTGGATGGCTTCATCTTCG TGGTGGCCCCAGATGGGAAGATCATGTACATTTCAGAGACAGCTTCAGTTCACCTGGGCCTTTCTCAGGT AGAGCTGACTGGGAACAGCATCTATGAATATATCCACCCGGCTGACCACGATGAGATGACAGCCGTGCTC ACAGCCCATCAGCCCTACCACTCTCACTTTGTACAGGAGTACGAGATCGAACGCTCCTTCTTTCTAAGGA TGAAGTGTGTTTTGGCCAAGCGGAATGCTGGCCTCACCTGCGGTGGCTACAAGGTCATTCACTGTAGTGG CTACCTGAAGATCCGCCAGTACAGCCTGGATATGTCTCCCTTTGATGGATGCTACCAAAATGTGGGCTTG GTGGCTGTGGGTCACTCCCTGCCTCCGAGTGCTGTGACAGAGATCAAACTACACAGCAACATGTTCATGT TCCGAGCCAGTCTGGATATGAAGCTTATTTTCCTGGACTCCAGGGTGGCAGAGCTGACTGGATATGAACC TCAGGACTTGATTGAGAAGACCCTGTACCATCACGTGCATGGCTGTGACACCTTCCACCTACGCTGTGCA CACCACTTACTGTTGGTGAAGGGACAAGTGACCACCAAGTACTACAGGTTCTTGGCGAAGCAGGGCGGCT GGGTCTGGGTGCAGAGTTACGCCACCATCGTACACAACAGCCGCTCCTCCAGGCCGCACTGCATCGTCAG CGTCAACTACGTCCTCACAGACACAGAATACAAAGGGCTACAGCTCTCCCTGGACCAGATATCAGCCTCT AAGCCCACTTTCTCCTATACCAGCAGTTCCACTCCCACTATCTCGGACAACAGGAAGGGGGCCAAGTCAA GGCTCTCCAGTAGCAAGTCAAAATCTAGGACCTCCCCATATCCCCAGTATTCGGGATTCCATACTGAGAG ATCGGAATCTGACCATGACAGCCAGTGGGGTGGAAGTCCCCTGACTGATACTGCCTCCCCACAGCTCCTG GACCCAGAGAGGCCTGGCTCTCAGCACGAATTGTCCTGTGCGTATAGGCAGTTCCCAGATCGCAGTTCTC TCTGCTATGGTTTTGCTCTGGACCATTCCAGGCTGGTGGAGGACAGGCACTTCCACACCCAAGCCTGCGA GGGAGGACGGTGCGAGGCAGGCAGGTACTTCCTGGGAGCACCACCTACTGGGAGAGATCCCTGGTGGGGC TCTCGAGCAGCTTTGCCTCTTACCAAGGCCTCCCCGGAGAGCAGAGAAGCCTATGAGAACAGCATGCCTC ATATCACCTCCATCCACAGAATCCACGGTCGAGGTCACTGGGATGAAGATAGTGTGGTCAGCTCTCCAGA CCCTGGGTCAGCCAGTGAATCTGGTGACCGATACCGCACCGAACAGTATCAAAATAGCCCACATGAACCT AGCAAAATTGAAACTCTCATAAGAGCCACCCAGCAGATGATTAAAGAGGAAGAGAACAGATTACAGCTAA GGAAAGCCCCTCCAGACCAACTGGCTTCCATTAATGGAGCTGGGAAAAAACACTCCCTCTGTTTTGCAAA CTATCAACAGCCCCCACCCACAGGTGAGGTCTGCCACAGCTCTGCTCTTGCCAGCACTTCACCATGTGAC CATATCCAGCAGAGAGAGGGAAAGATGCTCAGCCCTCATGAAAATGACTATGACAACAGCCCCACTGCAC TGTCTCGGATAAGTAGTCCTAGTTCTGATCGCATTACAAAGTCTAGTTTAATCCTGGCTAAAGACTATCT ACATTCAGATATGTCTCCCCATCAGACAGCAGGAGACCATCCTGCCATCTCTCCAAACTGTTTTGGCTCC CACCGGCAGTATTTTGATAAGCATGCCTATACATTGACTGGTTATGCCCTGGAACACTTATATGACAGTG AGACTATTAGAAACTATTCCTTGGGCTGCAATGGCTCACACTTTGATGTGACTTCCCATCTAAGGATGCA ACCAGACCCAGCACAAGGACACAAGGGAACATCTGTTATAATAACCAATGGAAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR224997 representing NM_011376 Red=Cloning site Green=Tags(s) MKEKSKNAARTRREKENSEFYELAKLLPLPSAITSQLDKASIIRLTTSYLKMRVVFPEGLGEAWGHTSRT SPLDNVGRELGSHLLQTLDGFIFVVAPDGKIMYISETASVHLGLSQVELTGNSIYEYIHPADHDEMTAVL TAHQPYHSHFVQEYEIERSFFLRMKCVLAKRNAGLTCGGYKVIHCSGYLKIRQYSLDMSPFDGCYQNVGL VAVGHSLPPSAVTEIKLHSNMFMFRASLDMKLIFLDSRVAELTGYEPQDLIEKTLYHHVHGCDTFHLRCA HHLLLVKGQVTTKYYRFLAKQGGWVWVQSYATIVHNSRSSRPHCIVSVNYVLTDTEYKGLQLSLDQISAS KPTFSYTSSSTPTISDNRKGAKSRLSSSKSKSRTSPYPQYSGFHTERSESDHDSQWGGSPLTDTASPQLL DPERPGSQHELSCAYRQFPDRSSLCYGFALDHSRLVEDRHFHTQACEGGRCEAGRYFLGAPPTGRDPWWG SRAALPLTKASPESREAYENSMPHITSIHRIHGRGHWDEDSVVSSPDPGSASESGDRYRTEQYQNSPHEP SKIETLIRATQQMIKEEENRLQLRKAPPDQLASINGAGKKHSLCFANYQQPPPTGEVCHSSALASTSPCD HIQQREGKMLSPHENDYDNSPTALSRISSPSSDRITKSSLILAKDYLHSDMSPHQTAGDHPAISPNCFGS HRQYFDKHAYTLTGYALEHLYDSETIRNYSLGCNGSHFDVTSHLRMQPDPAQGHKGTSVIITNGS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_011376
ORF Size	2295 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_011376.3, NP_035506.2
RefSeq Size	7355 bp
RefSeq ORF	2298 bp
Locus ID	20464
UniProt ID	Q61045
MW	85.5 kDa
Gene Summary	Transcriptional factor that may have pleiotropic effects during embryogenesis and in the adult.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221432	Sim1 (untagged) - Mouse single-minded homolog 1 (Drosophila) (Sim1), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 9,880.00
MG224997	Sim1 (tGFP-tagged) - Mouse single-minded homolog 1 (Drosophila) (Sim1), (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,370.00
MR224997L3	Lenti ORF clone of Sim1 (Myc-DDK-tagged) - Mouse single-minded homolog 1 (Drosophila) (Sim1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
MR224997L4	Lenti ORF clone of Sim1 (mGFP-tagged) - Mouse single-minded homolog 1 (Drosophila) (Sim1)	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00

You may also need

Empty Vector

DDK/Flag Antibody

其它Sim1产品

Expression Plasmids

Lentiviral Particles

CRISPR

RNAi

Proteins

Antibodies