Mtdh (NM_026002) Mouse Tagged ORF Clone
CAT#: MR224759
- TrueORF®
Mtdh (Myc-DDK-tagged) - Mouse metadherin (Mtdh)
ORF Plasmid: tGFP
"NM_026002" in other vectors (4)
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CNY 4,320.00
CNY 4,560.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 3D3; 3D3/Lyric; 2610103J23Rik; AEG-1; AV353288; D8Bwg1112e; Lyric |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR224759 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTGCACGAAGCTGGCAGGACGAGCTGGCCCAGCAGGCCGAGGAGGGCTCTGCCCGGCTGCGGGAGT TGCTCTCGGTCGGCCTAGGTTTTCTGCGCACGGAGTTGGGCCTCGACCTGGGGCTAGAGCCGAAGCGGTA CCCGGGCTGGGTGATCCTGGTGGGCACCGGCGCTCTCGGGCTGCTCCTGCTCTTCCTTCTAGGTTACGGC TGGGCCGCGGCTTGCGCCGGCGCCCGCAAGAAGCGAAGGAGCCCGCCCCGCAAGCGGGAGGAGGCGGCCC CGCCGACTCCGGCCCCCGACGACCTAGCCCAGCTGAAGAATCTCAGAAGCGAGGAGCAAAAGAAGAAGAA CCGGAAGAAGCTTCCTGAAAAGCCCAAACCAAATGGACGGACTGTTGAAGTACCCGAGGATGAAGTTGTT AGAAATCCCCGAAGTATAACTGCAAAACAAGCACCAGAGACAGACAAGAAAAATGAAAAGTCAAAGAAAA ATAAGAAGAAATCAAAGTCAGATGCTAAAGCAGTGCAAAACAGTTCACGCCATGATGGAAAGGAAGTTGA TGAAGGAGCCTGGGAAACTAAAATTAGTCACAGAGAGAAACGACAACAGCGTAAACGTGATAAAGTGCTG ACTGATTCTGGTTCATTGGATTCAACTATCCCTGGGATAGAAAATATCATCACAGTTACCACCGAGCAAC TTACAACTGCATCATTTCCTGTTGGTTCCAAGAAGAATAAAGGTGATTCTCATCTAAATGTTCAAGTTAG CAACTTTAAGTCTGGAAAAGGAGATTCTACACTGCAGGTTTCTTCAAGGCTGAATGAAAATCTTACTGTC AATGGAGGAGGCTGGAGTGAAAAGTCTGTAAAACTCTCCTCACAATTGAGTGAGGAGAAGTGGAACTCTG TCCCACCTGCTTCTGCAGGCAAGAGGAAAACAGAGCCATCGGCTTGGACTCAAGACACTGGTGACACTAA TGCAAATGGGAAAGACTGGGGAAGGAATTGGAGTGATCGCTCAATATTTTCTGGCATTGGATCTACTGCT GAGCCAGTTTCTCAGTCTACCACTTCTGATTATCAGTGGGATGTTAGCCGTAATCAACCTTATATCGATG ATGAATGGTCTGGGTTAAATGGTTTGTCTTCTGCTGACCCTAGCTCAGACTGGAATGCACCAGCAGAGGA GTGGGGGAACTGGGTAGATGAAGATAGAGCTTCACTTCTGAAGTCCCAGGAACCAATTTCTAATGATCAA AAGGTTTCAGATGATGATAAAGAAAAAGGGGAGGGAGCTCTTCCAACTGGAAAATCTAAAAAGAAAAAGA AGAAAAAGAAGAAGCAAGGGGAAGATAACTCTCACACACAGGACACAGAAGACCTAGAAAAGGACACTAG AGAAGAGCTTCCAGTGAATACCTCAAAAGCCCGACCAAAACAGGAGAAAGCTTGTTCCCTGAAGACCATG AGCACTAGTGACCCAGCTGAAGTACTCATCAAAAATAGCCAGCCTGTCAAGACTCTTCCTCCTGCTATCT CTGCCGAGCCATCTATTACCTTATCAAAAGGTGACTCTGACAACAGCTCTTCCCAAGTGCCACCGATGTT ACAAGACACAGACAAGCCCAAGTCAAATGCTAAGCAAAACAGTGTGCCTCCCTCACAGACCAAGTCTGAA ACTAACTGGGAATCTCCAAAACAAATAAAAAAGAAGAAAAAGGCCAGACGGGAAACG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224759 protein sequence
Red=Cloning site Green=Tags(s) MAARSWQDELAQQAEEGSARLRELLSVGLGFLRTELGLDLGLEPKRYPGWVILVGTGALGLLLLFLLGYG WAAACAGARKKRRSPPRKREEAAPPTPAPDDLAQLKNLRSEEQKKKNRKKLPEKPKPNGRTVEVPEDEVV RNPRSITAKQAPETDKKNEKSKKNKKKSKSDAKAVQNSSRHDGKEVDEGAWETKISHREKRQQRKRDKVL TDSGSLDSTIPGIENIITVTTEQLTTASFPVGSKKNKGDSHLNVQVSNFKSGKGDSTLQVSSRLNENLTV NGGGWSEKSVKLSSQLSEEKWNSVPPASAGKRKTEPSAWTQDTGDTNANGKDWGRNWSDRSIFSGIGSTA EPVSQSTTSDYQWDVSRNQPYIDDEWSGLNGLSSADPSSDWNAPAEEWGNWVDEDRASLLKSQEPISNDQ KVSDDDKEKGEGALPTGKSKKKKKKKKKQGEDNSHTQDTEDLEKDTREELPVNTSKARPKQEKACSLKTM STSDPAEVLIKNSQPVKTLPPAISAEPSITLSKGDSDNSSSQVPPMLQDTDKPKSNAKQNSVPPSQTKSE TNWESPKQIKKKKKARRET TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_026002 |
ORF Size | 1740 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_026002.2, NM_026002.3, NM_026002.4, NP_080278.3 |
RefSeq Size | 3680 bp |
RefSeq ORF | 1740 bp |
Locus ID | 67154 |
UniProt ID | Q80WJ7 |
MW | 63.8 kDa |
Gene Summary | Downregulates SLC1A2/EAAT2 promoter activity when expressed ectopically. Activates the nuclear factor kappa-B (NF-kappa-B) transcription factor. Promotes anchorage-independent growth of immortalized melanocytes and astrocytes which is a key component in tumor cell expansion. Promotes lung metastasis and also has an effect on bone and brain metastasis, possibly by enhancing the seeding of tumor cells to the target organ endothelium. Induces chemoresistance (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC219308 | Mtdh (untagged) - Mouse metadherin (Mtdh), (10ug) |
CNY 7,410.00 |
|
MG224759 | Mtdh (tGFP-tagged) - Mouse metadherin (Mtdh), (10ug) |
CNY 5,040.00 |
|
MR224759L3 | Lenti ORF clone of Mtdh (Myc-DDK-tagged) - Mouse metadherin (Mtdh) |
CNY 6,460.00 |
|
MR224759L4 | Lenti ORF clone of Mtdh (mGFP-tagged) - Mouse metadherin (Mtdh) |
CNY 6,460.00 |