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Cbfa2t3 (NM_001109873) Mouse Tagged ORF Clone
CAT＃: MR224532
- TrueORF®
Cbfa2t3 (Myc-DDK-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2
ORF Plasmid: tGFP
"NM_001109873" in other vectors (4)
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CNY 4,360.00
CNY 5,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A630044F12Rik; AI465270; AW229127; Cbfa2t3h; ETO-2; Eto2; MTGR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224532 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCAGACTCCCCGGCTGAAGTGAAGACGCAGCCCCGGTCCACACCCCCCAGCATGCCGCCCCCACCTC CCACCTCATCCCAGGGAGCCACACGACCACCCTCCTTCACACCTCACACACATGGCGAGGATGGACCTGC GACGTCTCTTCCCCATGGCCGTTTCCACGGCTGCTTAAAGTGGTCCATGGTCTGTCTCTTGATGAACGGC AGCAGCCACTCACCCACGGCCATCCACGGTGCCCCATCTACACCCAATGGCTTCAGCAACGGCCCAGCCA CCTCATCCACGGCCTCGCTCTCCACACAGCACCTGCCCCCGGCGTGCGGGGCACGGCAGCTCAGCAAGCT TAAGCGTTTCCTCACCACCCTACAGCAGTTTGGCAGTGACATCTCACCTGAGATCGGGGAGCGCGTGCGC ACACTGGTGCTGGGCCTGGTGAACTCAACTCTGACGATCGAAGAGTTTCATGCCAAGCTCCAGGAAGCCA CCAACTTTCCACTGAGGCCGTTTGTTATCCCTTTTCTGAAGGCTAATCTTCCACTGCTGCAGCGTGAGCT CCTGCACTGTGCCCGCCTGGCCAAACAGACACCTGCCCAGTACCTGGCCCAGCACGAACAGCTGCTGCTG GACGCCAGCGCCACCTCCCCTGTCGACTCGTCTGAGCTCCTGCTGGAAGTCAACGAGAACGGCAAAAGGA GAACACCTGACAGGACCAAAGAGAATGGATCAGACCGGGACCCTCTGCACCCCGACCACCTCAGTAAGCG GTCCTGCACCCTGAGCCCCGCCCAGCGCTGCAGCCCCAGCAATGGGCTGCCCCACCCGACGCCACCCCCA CCCCCGCACTATCGCCTGGAGGACATGGCCATGGCCCACCATTTCCGGGACTCCTACCGCCATCCTGATC CCCGAGAGCTACGGGAACGCCACCGGCCCCTGGCCATACCTGGGTCTCGACAAGAAGAAGTGATTGATCA CAGGCTCACAGAACGCGAGTGGGCAGAAGAATGGAAGCACCTCAACAGTCTTCTGAACTGCATCATGGAC ATGGTGGAGAAGACCCGGCGATCCCTCACCGTCCTGCGCCGGTGTCAGGAGGCCGACCGTGAGGAACTCA ACCACTGGATCCGGTGCTACAGTGACTCTGAGGAGGGGAAGAAGGGCCCTACCCCCATCTCTGCCCGGTC CCTCAACAGCTGCAGTGGCCCTGAGGGGTCTCAGCTAGATGTTCACCGGGACTTCACGCCCAGGACCCTG TCTGGCTACATGCCTGAAGAGATCTGGAGGAAGGCTGAAGAAGCTGTGAATGAGGTGAAGCGCCAGGCCA TGTCAGAACTACAGAAAGCTGTGTCTGATGCGGAGCGCAAAGCCCATGAACTCATCACCACAGAGCGTGC CAAGATGGAACGAGCCCTGGCGGAGGCCAAGCGACAGGCCTCGGAGGATGCCCTGACTGTCATCAACCAG CAAGAGGACTCCAGCGAGAGCTGCTGGAACTGCGGGCGCAAGGCCAGCGAGACGTGCAGTGGCTGTAACG CCGCCCGCTACTGCGGGTCCTTCTGTCAGCACAAAGACTGGGAGAAACACCATCACGTGTGCGGCCAGAG TCTGCAGGGCCCCGCGGCTGCAGTGGCTGACCCACTACCTGGACAGCCTGACGCCACTGCCAGCCCCAGC GAAGCCGGCTCGGCAGGGCCCTCTCGTCCCTGCTCTCCGGGGCCGCCAGGCCCGCTGGACGCTGCTGTGC CCCGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224532 protein sequence
Red=Cloning site Green=Tags(s) MPDSPAEVKTQPRSTPPSMPPPPPTSSQGATRPPSFTPHTHGEDGPATSLPHGRFHGCLKWSMVCLLMNG SSHSPTAIHGAPSTPNGFSNGPATSSTASLSTQHLPPACGARQLSKLKRFLTTLQQFGSDISPEIGERVR TLVLGLVNSTLTIEEFHAKLQEATNFPLRPFVIPFLKANLPLLQRELLHCARLAKQTPAQYLAQHEQLLL DASATSPVDSSELLLEVNENGKRRTPDRTKENGSDRDPLHPDHLSKRSCTLSPAQRCSPSNGLPHPTPPP PPHYRLEDMAMAHHFRDSYRHPDPRELRERHRPLAIPGSRQEEVIDHRLTEREWAEEWKHLNSLLNCIMD MVEKTRRSLTVLRRCQEADREELNHWIRCYSDSEEGKKGPTPISARSLNSCSGPEGSQLDVHRDFTPRTL SGYMPEEIWRKAEEAVNEVKRQAMSELQKAVSDAERKAHELITTERAKMERALAEAKRQASEDALTVINQ QEDSSESCWNCGRKASETCSGCNAARYCGSFCQHKDWEKHHHVCGQSLQGPAAAVADPLPGQPDATASPS EAGSAGPSRPCSPGPPGPLDAAVPR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001109873 |
| ORF Size | 1755 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq Size | 7678 bp |
| RefSeq ORF | 1758 bp |
| Locus ID | 12398 |
| UniProt ID | O54972 |
| MW | 64.5 kDa |
| Gene Summary | Transcriptional corepressor which facilitates transcriptional repression via its association with DNA-binding transcription factors and recruitment of other corepressors and histone-modifying enzymes. Can repress the expression of MMP7 in a ZBTB33-dependent manner. Reduces the protein levels and stability of the transcriptinal regulator HIF1A; interacts with EGLN1 and promotes the HIF1A prolyl hydroxylation-dependent ubiquitination and proteasomal degradation pathway. Contributes to inhibition of glycolysis and stimulation of mitochondrial respiration by down-regulating the expression of glycolytic genes including PFKFB3, PFKFB4, PDK1, PFKP, LDHA and HK1 which are direct targets of HIF1A (By similarity). Regulates the proliferation and the differentiation of erythroid progenitors by repressing the expression of TAL1 target genes (PubMed:16407974). Plays a role in granulocyte differentiation (PubMed:15231665).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219266 | Cbfa2t3 (untagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2, (10ug) |
CNY 7,410.00 |
|
| MG224532 | Cbfa2t3 (tGFP-tagged) - Mouse core-binding factor runt domain alpha subunit 2 translocated to 3 (human) (Cbfa2t3) transcript variant 2, (10ug) |
CNY 5,610.00 |
|
| MR224532L3 | Lenti ORF clone of Cbfa2t3 (Myc-DDK-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2 |
CNY 7,030.00 |
|
| MR224532L4 | Lenti ORF clone of Cbfa2t3 (mGFP-tagged) - Mouse core-binding factor, runt domain, alpha subunit 2, translocated to, 3 (human) (Cbfa2t3), transcript variant 2 |
CNY 7,030.00 |
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