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Sgca (NM_009161) Mouse Tagged ORF Clone
CAT＃: MR224511
- TrueORF®
Sgca (Myc-DDK-tagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 2
ORF Plasmid: tGFP
"NM_009161" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 50DA; 50DAG; A; ad; Asg |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224511 representing NM_009161
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAGCAGCAGTAACTTGGATACCTCTCCTGGCAGGTCTCCTGGCAGGACTGAGGGACACCAAGGCCC AGCAGACAACTTTACACCTACTTGTGGGTCGTGTGTTTGTGCATCCTTTGGAACATGCCACCTTCCTGCG CCTTCCAGAACACGTTGCGGTGCCACCCACTGTCCGACTCACCTACCACGCTCACCTCCAGGGACATCCA GACCTGCCCAGGTGGCTGCACTACACACAGCGCAGTCCCTATAACCCTGGCTTCCTCTACGGCTCCCCCA CTCCAGAAGATCGTGGGTACCAAGTCATCGAGGTCACAGCCTACAATCGAGACAGTTTTGACACCACTAG ACAGAGGCTGCTGCTGCTGATTGGGGACCCCGAAGGTCCCCGGTTGCCATACCAAGCTGAGTTCCTGGTG CGCAGCCATGATGTGGAGGAGGTGCTGCCCACCACACCTGCCAACCGCTTCCTCACCGCCTTGGGGGGAC TGTGGGAGCCAGGAGAGCTTCAGCTGCTCAACATCACTTCCGCCTTGGACCGGGGAGGCCGAGTCCCTCT TCCTATTGAGGGACGGAAGGAAGGGGTATACATTAAGGTAGGCTCTGCCACACCCTTCTCCACCTGCCTG AAGATGGTGGCGTCGCCCGACAGCTATGCCCGTTGTGCCCAGGGACAGCCTCCACTACTGTCCTGCTACG ACACTTTGGCACCCCACTTCCGCGTTGACTGGTGCAATGTGTCTCTGGTAGACAAGTCAGTACCCGAGCC CCTGGATGAGGTACCTACTCCAGGCGATGGGATCTTGGAGCACGACCCGTTCTTCTGCCCACCCACTGAA GCCACAGACCGAGACTTCCTGACAGATGCCTTGGTGACCCTCTTGGTGCCTTTGTTGGTGGCTCTGCTGC TTACTCTGTTGCTGGCTTACATCATGTGCTTTCGGCGTGAAGGACGGCTGAAGAGAGACATGGCCACCTC TGACATCCAGATGTTTCACCACTGTTCCATCCATGGGAATACAGAAGAGCTTCGGCAGATGGCAGCCAGC CGAGAGGTGCCCCGGCCTCTTTCCACCTTGCCCATGTTTAATGTTCGTACAGGAGAGCGGTTACCTCCCC GAGTAGACAGCGCACAGATGCCTCTTATCCTGGACCAGCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224511 representing NM_009161
Red=Cloning site Green=Tags(s) MAAAVTWIPLLAGLLAGLRDTKAQQTTLHLLVGRVFVHPLEHATFLRLPEHVAVPPTVRLTYHAHLQGHP DLPRWLHYTQRSPYNPGFLYGSPTPEDRGYQVIEVTAYNRDSFDTTRQRLLLLIGDPEGPRLPYQAEFLV RSHDVEEVLPTTPANRFLTALGGLWEPGELQLLNITSALDRGGRVPLPIEGRKEGVYIKVGSATPFSTCL KMVASPDSYARCAQGQPPLLSCYDTLAPHFRVDWCNVSLVDKSVPEPLDEVPTPGDGILEHDPFFCPPTE ATDRDFLTDALVTLLVPLLVALLLTLLLAYIMCFRREGRLKRDMATSDIQMFHHCSIHGNTEELRQMAAS REVPRPLSTLPMFNVRTGERLPPRVDSAQMPLILDQH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009161 |
| ORF Size | 1161 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009161.4, NP_033187.1 |
| RefSeq Size | 1428 bp |
| RefSeq ORF | 1164 bp |
| Locus ID | 20391 |
| UniProt ID | P82350 |
| MW | 43.7 kDa |
| Gene Summary | This gene encodes a member of the sarcoglycan alpha/epsilon family of transmembrane proteins. The encoded protein is part of the dystrophin-glycoprotein complex which links the extracellular matrix to the cytoskeleton in muscle fibers. Disruption of this gene results in progressive muscular dystrophy and is associated with the development of embryonal rhabdomysarcoma. [provided by RefSeq, Dec 2012] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209386 | Sgca (untagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG224511 | Sgca (tGFP-tagged) - Mouse sarcoglycan alpha (dystrophin-associated glycoprotein) (Sgca) transcript variant 2, (10ug) |
CNY 3,140.00 |
|
| MR224511L3 | Lenti ORF clone of Sgca (Myc-DDK-tagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 2 |
CNY 4,750.00 |
|
| MR224511L4 | Lenti ORF clone of Sgca (mGFP-tagged) - Mouse sarcoglycan, alpha (dystrophin-associated glycoprotein) (Sgca), transcript variant 2 |
CNY 4,750.00 |
