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Ptch2 (NM_008958) Mouse Tagged ORF Clone
CAT＃: MR224361
- TrueORF®
Ptch2 (Myc-DDK-tagged) - Mouse patched homolog 2 (Ptch2)
ORF Plasmid: tGFP
"NM_008958" in other vectors (4)
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CNY 8,232.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ptc; ptc2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224361 representing NM_008958
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTTCGGCCACTGTCCCTTGGAGAGCTACCTCCCAGCTACACACCTCCAGCTCGGTCCTCCGCACCTC ATATCCTAGCTGGGAGCCTGCAGGCTCCTCTCTGGCTTCGAGCTTACTTCCAAGGTCTACTCTTCTCCCT GGGGTGCAGGATCCAGAAACACTGCGGCAAAGTGCTCTTCCTGGGACTGGTGGCCTTTGGGGCTCTGGCA CTGGGTCTCCGAGTGGCTGTAATTGAGACAGACCTAGAACAGCTCTGGGTAGAAGTGGGCAGCAGGGTGA GCCAGGAGCTGCACTACACCAAGGAGAAGCTGGGGGAAGAGGCTGCGTACACCTCCCAGATGTTGATTCA GACTGCGCACCAGGAAGGGGGAAACGTCCTCACCCCCGAGGCACTTGACTTGCACCTCCAGGCGGCGCTC ACTGCCAGTAAAGTCCAAGTATCACTCTATGGGAAATCCTGGGATTTGAACAAGATCTGCTACAAATCAG GGGTTCCCCTTATTGAAAATGGGATGATCGAGCGGATGATTGAGAAGCTGTTTCCCTGTGTGATCCTCAC CCCGCTTGACTGCTTCTGGGAAGGAGCCAAACTCCAAGGGGGCTCTGCCTACTTGCCGGGCCGCCCTGAT ATCCAGTGGACCAACCTGGACCCTCAGCAGCTGCTCGAGGAGCTGGGCCCCTTTGCCTCTCTGGAGGGCT TCCGGGAACTGCTAGATAAGGCACAGGTGGGCCAGGCCTATGTGGGGCGACCCTGTCTGGACCCTGATGA CCCCCACTGTCCACCTAGTGCTCCCAACCGGCACAGCAGGCAGGCTCCCAATGTGGCTCAGGAGCTGAGT GGGGGCTGCCATGGCTTCTCCCACAAGTTCATGCACTGGCAGGAGGAACTGCTACTAGGGGGCACAGCCA GAGATCTCCAAGGACAGCTGCTGAGGGCAGAGGCCTTGCAGAGCACCTTCCTGCTCATGAGTCCCCGTCA GCTGTACGAGCACTTCCGGGGCGACTACCAGACACATGACATCGGCTGGAGCGAGGAGCAGGCCAGCATG GTGCTGCAGGCCTGGCAGAGGCGCTTTGTGCAGCTAGCCCAGGAGGCTCTGCCGGCCAACGCGTCCCAGC AGATCCATGCCTTCTCCTCCACCACCCTGGATGACATCCTGCGCGCGTTCTCTGAAGTCAGCACCACCCG TGTGGTAGGAGGCTATCTGCTTATGCTGGCCTATGCCTGCGTAACAATGCTGCGGTGGGACTGCGCCCAG TCCCAGGGTGCCGTAGGTCTTGCTGGGGTGTTGCTGGTGGCCCTGGCGGTGGCCTCAGGTCTTGGGCTTT GTGCCCTACTTGGCATCACTTTCAATGCTGCCACTACACAGGTGCTGCCCTTCTTGGCTCTGGGCATTGG CGTGGATGACATCTTCCTGCTGGCGCATGCCTTCACAAAAGCCCCACCTGACACCCCTCTCCCAGAGCGT ATGGGTGAGTGTTTGAGGAGCACGGGCACCAGCGTGGCACTCACATCCGTCAACAACATGGTCGCCTTTT TCATGGCTGCCCTGGTTCCCATTCCTGCGCTGCGGGCCTTCTCCCTGCAGGCAGCCATAGTGGTCGGCTG CAACTTTGCAGCGGTAATGCTCGTCTTCCCTGCCATCCTCAGCCTCGACCTGCGCCGGCGCCACCGCCAA CGCCTGGACGTTCTCTGCTGCTTTTCTAGCCCCTGCTCCGCTCAGGTCATTCAGATGCTGCCCCAGGAGC TAGGGGACAGAGCAGTACCAGTGGGCATTGCCCACCTGACTGCCACCGTGCAAGCCTTCACCCACTGCGA AGCCAGCAGCCAGCATGTAGTCACCATTTTGCCTCCTCAAGCCCACCTGCTGTCTCCAGCTTCTGACCCA CTGGGCTCCGAACTCTATAGCCCTGGAGGGTCTACACGGGACCTTCTCAGTCAGGAGGAGGGGACAGGGC CACAGGCGGCCTGCAGGCCCCTGCTCTGTGCCCACTGGACTCTCGCCCATTTTGCCCGCTATCAGTTTGC ACCTTTACTGCTCCAGACACGAGCCAAGGCCCTGGTGTTGCTGTTCTTTGGGGCTCTTTTGGGCCTGAGC CTCTATGGAGCCACCTTGGTACAAGATGGGCTGGCCCTGACAGATGTGGTCCCTAGGGGCACCAAGGAAC ATGCTTTCCTGAGCGCCCAGCTCAGGTACTTCTCCCTGTACGAGGTGGCTCTAGTGACACAGGGTGGCTT TGACTACGCCCACTCCCAACGCGCCCTCTTTGATCTGCACCAGCGCTTCAGCTCCCTCAAGGCTGTGCTG CCCCCACCTGCCACCCAGGCACCCCGCACCTGGCTTCACTACTACCGCAGCTGGCTACAGGGTATCCAGG CTGCATTTGACCAAGACTGGGCTTCTGGGCGCATCACCTGCCACTCTTACCGCAACGGCTCAGAAGATGG CGCCCTGGCCTACAAGCTGCTCATCCAAACCGGGAATGCCCAGGAGCCTCTGGATTTCAGCCAGCTGACC ACAAGGAAACTGGTGGACAAGGAGGGACTCATTCCCCCAGAGCTCTTCTACATGGGGCTAACCGTGTGGG TGAGCAGCGACCCCCTGGGCCTCGCAGCCTCTCAGGCCAACTTCTACCCCCCACCTCCAGAGTGGCTTCA CGACAAATATGATACCACCGGGGAGAACCTTCGCATCCCGGCAGCCCAGCCCTTGGAGTTTGCCCAGTTC CCCTTCCTGCTGCATGGACTCCAGAAGACTGCAGACTTTGTAGAAGCCATCGAAGGGGCCAGGGCGGCAT GCACAGAGGCAGGCCAGGCAGGGGTGCATGCCTACCCCAGTGGCTCCCCCTTCCTCTTCTGGGAGCAGTA TCTGGGTCTTCGGCGCTGCTTCCTGCTGGCAGTCTGCATCTTGCTGGTGTGCACCTTCCTCGTCTGTGCC CTGCTTCTACTCAGCCCGTGGACAGCTGGCCTCATAGTGCTGGTCCTGGCAATGATGACTGTGGAGCTCT TTGGTATCATGGGATTCCTGGGCATCAAACTGAGTGCCATCCCCGTGGTAATCCTCGTGGCCTCTATAGG CATTGGTGTTGAATTCACAGTTCACGTGGCTCTGGGCTTCCTGACCAGCCATGGTAGCCGGAACCTGCGG GCTGCTAGCGCTCTGGAACAGACCTTTGCCCCTGTGACTGATGGAGCTGTCTCCACCTTGCTGGGTCTGC TCATGCTTGCTGGTTCTAACTTTGACTTCATCATAAGGTATTTCTTTGTGGTGCTGACGGTGCTGACACT CTTGGGCCTGCTCCATGGACTCCTGCTGCTGCCTGTGCTGCTCTCTATCCTAGGCCCCCCGCCACAGGTG GTGCAGGTATACAAGGAGAGTCCACAGACCCTAAACTCCGCTGCTCCACAGAGAGGTGGGCTCAGGTGGG ACAGGCCCCCCACCCTGCCCCAGAGTTTTGCCAGAGTGACTACCTCCATGACTGTGGCCCTCCACCCACC ACCTCTGCCTGGAGCATACGTCCACCCAGCCTCCGAGGAGCCCACA AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC TGGATTACAAGGATGACGACGATAAGGTTTAA >MR224361 representing NM_008958
Red=Cloning site Green=Tags(s) MVRPLSLGELPPSYTPPARSSAPHILAGSLQAPLWLRAYFQGLLFSLGCRIQKHCGKVLFLGLVAFGALA LGLRVAVIETDLEQLWVEVGSRVSQELHYTKEKLGEEAAYTSQMLIQTAHQEGGNVLTPEALDLHLQAAL TASKVQVSLYGKSWDLNKICYKSGVPLIENGMIERMIEKLFPCVILTPLDCFWEGAKLQGGSAYLPGRPD IQWTNLDPQQLLEELGPFASLEGFRELLDKAQVGQAYVGRPCLDPDDPHCPPSAPNRHSRQAPNVAQELS GGCHGFSHKFMHWQEELLLGGTARDLQGQLLRAEALQSTFLLMSPRQLYEHFRGDYQTHDIGWSEEQASM VLQAWQRRFVQLAQEALPANASQQIHAFSSTTLDDILRAFSEVSTTRVVGGYLLMLAYACVTMLRWDCAQ SQGAVGLAGVLLVALAVASGLGLCALLGITFNAATTQVLPFLALGIGVDDIFLLAHAFTKAPPDTPLPER MGECLRSTGTSVALTSVNNMVAFFMAALVPIPALRAFSLQAAIVVGCNFAAVMLVFPAILSLDLRRRHRQ RLDVLCCFSSPCSAQVIQMLPQELGDRAVPVGIAHLTATVQAFTHCEASSQHVVTILPPQAHLLSPASDP LGSELYSPGGSTRDLLSQEEGTGPQAACRPLLCAHWTLAHFARYQFAPLLLQTRAKALVLLFFGALLGLS LYGATLVQDGLALTDVVPRGTKEHAFLSAQLRYFSLYEVALVTQGGFDYAHSQRALFDLHQRFSSLKAVL PPPATQAPRTWLHYYRSWLQGIQAAFDQDWASGRITCHSYRNGSEDGALAYKLLIQTGNAQEPLDFSQLT TRKLVDKEGLIPPELFYMGLTVWVSSDPLGLAASQANFYPPPPEWLHDKYDTTGENLRIPAAQPLEFAQF PFLLHGLQKTADFVEAIEGARAACTEAGQAGVHAYPSGSPFLFWEQYLGLRRCFLLAVCILLVCTFLVCA LLLLSPWTAGLIVLVLAMMTVELFGIMGFLGIKLSAIPVVILVASIGIGVEFTVHVALGFLTSHGSRNLR AASALEQTFAPVTDGAVSTLLGLLMLAGSNFDFIIRYFFVVLTVLTLLGLLHGLLLLPVLLSILGPPPQV VQVYKESPQTLNSAAPQRGGLRWDRPPTLPQSFARVTTSMTVALHPPPLPGAYVHPASEEPT SGPTRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008958 |
| ORF Size | 3546 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008958.3, NP_032984.1 |
| RefSeq Size | 3549 bp |
| RefSeq ORF | 3549 bp |
| Locus ID | 19207 |
| UniProt ID | O35595 |
| MW | 129 kDa |
| Gene Summary | This gene encodes a member of the patched family of transmembrane receptor proteins. The encoded protein may be a functional receptor for the morphogen sonic hedgehog (Shh) and is reportedly involved in limb and skin development. Homozygous mutant mice for this gene exhibit hair loss and epidermal hyperplasia. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Aug 2015] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Ptch2/Gas1 and Ptch1/Boc differentially regulate Hedgehog signalling in murine primordial germ cell migration
,Kim, Y;Lee, J;Seppala, M;Cobourne, MT;Kim, SH;,
Nat Commun
,PubMed ID 32332736
[PTCH2]
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Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223858 | Ptch2 (untagged) - Mouse patched homolog 2 (Ptch2), (10ug) |
CNY 15,200.00 |
|
| MG224361 | Ptch2 (tGFP-tagged) - Mouse patched homolog 2 (Ptch2), (10ug) |
CNY 11,400.00 |
|
| MR224361L3 | Lenti ORF clone of Ptch2 (Myc-DDK-tagged) - Mouse patched homolog 2 (Ptch2) |
CNY 12,260.00 |
|
| MR224361L4 | Lenti ORF clone of Ptch2 (mGFP-tagged) - Mouse patched homolog 2 (Ptch2) |
CNY 12,260.00 |
