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Nfat5 (NM_018823) Mouse Tagged ORF Clone
CAT＃: MR224219
- TrueORF®
Nfat5 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant b
ORF Plasmid: tGFP
"NM_018823" in other vectors (4)
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CNY 10,616.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AI225870; B130038B15Rik; CAG-8; CAG80; mKIAA0827; NFATL1; nfatz; OREBP; TonEBP |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224219 representing NM_018823
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCCTCGGACTTCATCTCATTGCTCAGCGCGGACCTAGACCTGGAATCGCCCAAGTCCCTGTACTCGC GAGATTCTCTGAAGTTACACCCATCACAGACTTTTCATAGAGCTGGACTATTGGAAGAATCTGTCTATGA TCTTCTCCCAAAGGAGTTACAGTTACCTCCACCTAGAGAAACATCTGTAGCATCAATGAGTCAGACAAGC GGTGGTGAGGCAGGCTCGCCTCCTCCAGCTGTAGTTGCTGCTGATGCTTCTTCAGCTCCCTCCTCTTCCT CCATGGGCGGTGCTTGCAGCTCCTTTACCACCTCTTCCAGCCCTACCATTTATTCTACCTCAGTCACCGA CAGCAAGGCTATGCAAGTGGAGAGCTGCTCCTCAGCCGTGGGGGTAAGTAACAGAGGGGTAAGTGAAAAG CAGTTAACCGGTAACACAGTTCAGCAGCATCCATCAACCCCGAAGAGGCACACAGTTTTGTACATCTCAC CACCACCTGAGGACTTGCTGGATAACAGTCGGATGTCCTGCCAGGATGAGGGGTGTGGATTGGAATCTGA GCAGAGCTGCAGTATGTGGATGGAGGATTCCCCCTCCAACTTCAGTAACATGAGCACCAGTTCCTACAAT GATAACACTGAGGTACCTCGTAAATCACGAAAACGAAATCCAAAGCAGAGGCCGGGGGTCAAACGACGAG ATTGTGAAGAATCTAATATGGATATATTTGATGCCGACAGTGCCAAAGCACCTCACTATGTGCTTTCTCA GCTTACCACGGACAACAAAGGCAACTCAAAAGCTGGAAATGGAACATTGGACAGCCAAAAGGGAACTGGA GTAAAGAAGAGCCCTATGTTGTGCGGACAGTATCCGGTTAAAAGTGAGGGGAAGGAGCTGAAGATAGTGG TACAGCCTGAAACCCAACACCGAGCCCGGTACCTGACAGAGGGCAGCCGAGGCTCTGTAAAAGACAGAAC ACAGCAAGGCTTTCCTACGGTGAAGCTGGAAGGCCATAATGAACCAGTGGTGTTGCAGGTATTTGTGGGC AATGATTCTGGTCGAGTAAAACCACATGGATTCTATCAGGCCTGTAGAGTAACTGGGCGAAATACAACTC CCTGCAAAGAAGTGGACATTGAAGGTACCACTGTTATAGAAGTTGGTCTTGATCCTAGCAACAACATGAC ACTGGCGGTGGACTGTGTTGGAATATTGAAGCTGAGGAATGCCGATGTTGAAGCCAGAATTGGAATTGCT GGATCTAAGAAAAAGAGCACTCGTGCCAGATTGGTTTTTCGAGTTAATATCACAAGGAAAGATGGCTCTA CTCTGACATTGCAGACACCTTCTTCCCCCATTTTATGCACTCAGCCAGCAGGAGTTCCTGAGATCTTAAA GAAAAGCTTGCATAGCTGTTCAGTGAAAGGAGAGGAAGAAGTATTTTTAATTGGGAAAAACTTTCTGAAA GGAACTAAAGTTATTTTCCAGGAAAATGTTTCTGATGAAAACTCTTGGAAATCAGAAGCTGAAATTGACA TGGAGTTATTCCATCAGAACCATCTTATTGTGAAGGTTCCTCCGTATCATGACCAACATATAACTTTGCC TGTATCAGTGGGAATATATGTTGTGACCAATGCTGGAAGATCTCATGATGTTCAGCCATTTACGTACACT CCGGATCCAGCAGCTGGTGCTTTGAATGTAAATGTGAAAAAGGAGATATCTAGTCCAGCAAGACCTTGCT CTTTTGAAGAAGCCATGAAAGCAATGAAAACGACTGGATGTAACGTAGATAAGGTGACTATCCTTCCTAA TGCCCTGATCACTCCACTCATATCAAGCAGTATGATTAAGACTGAAGATGTTACTCCAATGGAAGTAACT TCAGAAAAAAGATCTTCCCCAATTTTTCAGACTACAAAGAGCATTGGATCAACCCAGCAAACCTTAGAAA CTATTTCTAACATAGCGGGGGGTGCACCCTTCTCCTCACCGTCGTCATCTTCCCACTTAACTCCTGAAAG TGAGAATCAGCAGCAGCTTCAGCCCAAGGCATACAACCCAGAGACCCTGACAACTATCCAGACACAGGAC ATCTCACAGCCCGGGACCTTTCCCGCAGTTTCTGCTGCTAGTCAGCTGCCCAGCAGCGATGCACTACTGC AGCAGGCAACACAGTTTCAGACAAGAGAAGCTCAGTCCAGAGACACAATACAGTCAGATACAGTGGTTAA CTTGTCACAGTTAACTGAAGCATCACAGCAGCAGCAATCCCCACTACAAGAACAAGCACAAACGTTACAG CAGCAGATACCATCAAATATTTTTCCATCGCCAAGCAGTGTCAGCCAGCTCCAGAGTACTATTCAGCAGC TGCAAGCAGGAAGTTTCACAGGCAGCGCTGCTGGCGGCAGGAGTGGAAGCGTTGATTTGGTCCAGCAGGT TTTAGAGGCACAACAGCAGTTATCGTCTGTTCTGTTTTCTACTCCAGATGGGAATGAGAATGTTCAAGAA CAGCTTAATGCAGACATTTTCCAAGTCAGTCAAATTCAAAATAGTGTAAGCCCTGGAATGTTTTCTTCAG CGGAGTCTGCAGTTCACACTAGACCAGATAACTTACTACCTGGGAGGGCTGACAGCGTCCATCAACAGAC TGAAAATACACTGTCTAATCAGCAGCAGCAGCAACAGCAGCAGCAGCAAGTGATGGAGTCATCAGCTGCA ATGGTGATGGAGATGCAGCAGAGCATTTGCCAAGCAGCTGCCCAGATCCAGTCAGAGCTGTTTCCTTCAG CTGCTTCAGCAAGTGGAAGCCTTCAGCAGTCTCCAGTTTACCAGCAGCCTTCTCACATGATGAGTGCACT GCCTACCAACGAGGACATGCAGATGCAGTGTGAGCTGTTCTCGTCTCCCCCTGCAGCTTCTGGAAACGAA ACTTCCACAACCACCACGCCACAGGTGGCAACCCCTGGCTCCACCATGTTTCAGACACCAAGTTCAGGAG ATGGAGAAGAAACTGGAGCACAAGCAAAACAGATTCAGAACAGCGTCTTTCAGACAATGGTCCAAATGCA GCGCAGTGGTGACAGCCAACCTCAAGTAAACCTCTTTTCATCTACAAAAAATATAATGAGTGTTCAGAAT AATGGTACCCAGCAACAAGGGAATAGCTTGTTCCAGCAAGGGAGCGAGATGATGTCACTTCAGTCTGGGA ACTTTTTGCAGCAGTCCTCTCATTCACAGGCTCAGCTCTTTCATCCTCAAAATCCTATTGCTGATGCTCA GAACCTTTCCCAGGAAACTCAAGGTTCAATTTTTCATAGTCCAAATCCTATTGTCCACAGTCAAACTTCC ACAGCATCCTCTGAACAACTGCAGCCTTCAATGTTTCACTCTCAGAATACCATTGCTGTGTTACAGGGCT CTTCAGTTCCTCAAGACCAGCAGTCACCCAACATATTTCTTTCCCAAAGTTCTATCAATAATCTTCAAAC TAACACAGTAGCCCAAGAAGAGCAGATTTCATTTTTTGCAGCACAGAACTCAATTTCTCCACTTCAGTCA ACATCAAACACTGAGCAGCAAGCTGCTTTCCAACAGCAGCCTCCAATCTCACACATCCAGACCCCTATTC TTTCCCAGGAACAGGCACAACCCTCTCAGCAAGGGTTATTTCAGCCTCAGGTGGCCCTTGGCTCCCTGCC TCCTAATCCAATGCCTCAAAACCAGCAAGGTCCAATTTTCCAAACACAGCGCCCAATAGTTGGCATGCAG AGTAACTCTCCATCCCAGGAGCAGCAGCAGCAGCAACAGCAGCAACAGCAGCAGCAGCAACAGCAACAAC AACAGCAACAGAGCATTTTATTCAGTAATCAGAATGCCATGGCTACAATGGCCTCCCAGAAGCAGCCACC ACCAAACATGATGTTTAGCCCAAACCAGAACCCAATGGCTAGTCAGGAGCAGCAGAACCAGTCAATTTTT CACCAGCAAAGTAATATGGCCCCAATGAACCAAGAGCAGCAGCCCATGCAATTTCAGAATCAGCCTACGG TTTCCTCACTTCAGAACCCAGGTCCTACGCAGTCTGAGTCACCACAGACCTCCTTGTTCCATAGCTCTCC TCAGATCCAGTTGGTTCAAGGGTCACCTAGTTCTCAAGATCAGCAAGTAACACTCTTCCTCTCTCCAGCA TCCATGTCTGCATTGCAAACCAGTATAAACCAACCAGACATGCAGCAGTCTCCTCTTTATTCCCCGCAGA ACAACATCCCTGGAATCCAAGGAAGCACTTCTTCACCCCAGCCACAGGCTACTTTATTTCACAACACTAC AGGAGGCACAATAAACCAAATACAAAATTCTCCTGGCTCATCTCAGCAGACTTCAGGAATGTTCTTATTT GGCATTCAAAATAATTGTAGTCAGCTTTTAACCTCCGGACCAGCTACATTGCCAGATCAGCTGATGGCCA TAAATCAGCAGGGCCAACCACAAAATGAGGGCCAATCTTCTGTGACAACACTTCTTTCTCAGCAAATGCC AGAGACTTCCCCACTGGCCTCCTCTGTGAACAGCAGTCAGAACATGGAAAAGATTGATTTGCTTGTTTCA TTGCAAAGCCAAGGGAACAATTTAACCGGCTCCTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224219 representing NM_018823
Red=Cloning site Green=Tags(s) MPSDFISLLSADLDLESPKSLYSRDSLKLHPSQTFHRAGLLEESVYDLLPKELQLPPPRETSVASMSQTS GGEAGSPPPAVVAADASSAPSSSSMGGACSSFTTSSSPTIYSTSVTDSKAMQVESCSSAVGVSNRGVSEK QLTGNTVQQHPSTPKRHTVLYISPPPEDLLDNSRMSCQDEGCGLESEQSCSMWMEDSPSNFSNMSTSSYN DNTEVPRKSRKRNPKQRPGVKRRDCEESNMDIFDADSAKAPHYVLSQLTTDNKGNSKAGNGTLDSQKGTG VKKSPMLCGQYPVKSEGKELKIVVQPETQHRARYLTEGSRGSVKDRTQQGFPTVKLEGHNEPVVLQVFVG NDSGRVKPHGFYQACRVTGRNTTPCKEVDIEGTTVIEVGLDPSNNMTLAVDCVGILKLRNADVEARIGIA GSKKKSTRARLVFRVNITRKDGSTLTLQTPSSPILCTQPAGVPEILKKSLHSCSVKGEEEVFLIGKNFLK GTKVIFQENVSDENSWKSEAEIDMELFHQNHLIVKVPPYHDQHITLPVSVGIYVVTNAGRSHDVQPFTYT PDPAAGALNVNVKKEISSPARPCSFEEAMKAMKTTGCNVDKVTILPNALITPLISSSMIKTEDVTPMEVT SEKRSSPIFQTTKSIGSTQQTLETISNIAGGAPFSSPSSSSHLTPESENQQQLQPKAYNPETLTTIQTQD ISQPGTFPAVSAASQLPSSDALLQQATQFQTREAQSRDTIQSDTVVNLSQLTEASQQQQSPLQEQAQTLQ QQIPSNIFPSPSSVSQLQSTIQQLQAGSFTGSAAGGRSGSVDLVQQVLEAQQQLSSVLFSTPDGNENVQE QLNADIFQVSQIQNSVSPGMFSSAESAVHTRPDNLLPGRADSVHQQTENTLSNQQQQQQQQQQVMESSAA MVMEMQQSICQAAAQIQSELFPSAASASGSLQQSPVYQQPSHMMSALPTNEDMQMQCELFSSPPAASGNE TSTTTTPQVATPGSTMFQTPSSGDGEETGAQAKQIQNSVFQTMVQMQRSGDSQPQVNLFSSTKNIMSVQN NGTQQQGNSLFQQGSEMMSLQSGNFLQQSSHSQAQLFHPQNPIADAQNLSQETQGSIFHSPNPIVHSQTS TASSEQLQPSMFHSQNTIAVLQGSSVPQDQQSPNIFLSQSSINNLQTNTVAQEEQISFFAAQNSISPLQS TSNTEQQAAFQQQPPISHIQTPILSQEQAQPSQQGLFQPQVALGSLPPNPMPQNQQGPIFQTQRPIVGMQ SNSPSQEQQQQQQQQQQQQQQQQQQQQSILFSNQNAMATMASQKQPPPNMMFSPNQNPMASQEQQNQSIF HQQSNMAPMNQEQQPMQFQNQPTVSSLQNPGPTQSESPQTSLFHSSPQIQLVQGSPSSQDQQVTLFLSPA SMSALQTSINQPDMQQSPLYSPQNNIPGIQGSTSSPQPQATLFHNTTGGTINQIQNSPGSSQQTSGMFLF GIQNNCSQLLTSGPATLPDQLMAINQQGQPQNEGQSSVTTLLSQQMPETSPLASSVNSSQNMEKIDLLVS LQSQGNNLTGSF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018823 |
| ORF Size | 4656 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018823.2, NP_061293.2 |
| RefSeq Size | 13389 bp |
| RefSeq ORF | 4659 bp |
| Locus ID | 54446 |
| UniProt ID | Q9WV30 |
| MW | 168.3 kDa |
| Gene Summary | Transcription factor involved in the transcriptional regulation of osmoprotective and inflammatory genes. Regulates hypertonicity-induced cellular accumulation of osmolytes.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224674 | Nfat5 (untagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant b, (10ug) |
CNY 19,950.00 |
|
| MG224219 | Nfat5 (tGFP-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5) transcript variant b, (10ug) |
CNY 12,216.00 |
|
| MR224219L3 | Lenti ORF clone of Nfat5 (Myc-DDK-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant b |
CNY 15,490.00 |
|
| MR224219L4 | Lenti ORF clone of Nfat5 (mGFP-tagged) - Mouse nuclear factor of activated T-cells 5 (Nfat5), transcript variant b |
CNY 15,490.00 |
