Peg3 (NM_008817) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AL022617; ASF-1; End4; Gcap4; mKIAA0287; Pw1; Zfp102
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR224127 representing NM_008817, codon optimized. Due to the complexity of NM_008817, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTACCACCACGAAGACGATACCAATTCAGACATGAACTCAGATGACGACATGAGTCGCAGCGGCAGGG AAACTCCCCCTCCAAGGCCATCTCATGCTTTCGGGTCCGAGCGCGACCTGGAAAGGCGCGGTCGGTCCAG AGACGTGGAGCCAAGAGACAGATGGCCGTATACACGGAACCCCCGCTCTAGATTGCCCCAAAGGGACCTG AGCCTGCCCGTGATGAGTCGGCCACACTTTGGTCTGGACCGCGATGACGATCGCAGGTCAATGGACTATG AGTCACGGTCACAAGACGCGGAGAGCTACCAGAATGTGGTCGAGCTGAAAGAGGATAAAAAGCCCCAGAA CCCCATTCAGGACAATTTGGAGAACTACCGCAAGCTCCTGAGCCTGGGGGTTCAGCTGGCGGAGGACGAC CGACACTCCCACATGACGCAGGGGCACTCAAGTCGGAGTAAGCGCACCGCTTACCCTAGTACTAGTAGGG GCCTGAAGCCAATGCCAGAAGCCAAGAAGCCCAGTCACCGGCGAGGAATTTGCGAGGACGAAAGTTCTCA CGGCGTTATTATGGAAAAATTTATTAAGGATGTGGCTAGAAACCCCAAGAGTGGTCGAGCCCGGGAGCTG AACGAACGCCCTCCACCTCGGTTCCCCAGACCGAACGACAACTGGAAGGACAGCAGTTCCTCACGCCGCG AGAGTGTCATTCAGGAGAGAGGATATGAAGGCAGCGCTTTCCGCGGCGGCTTCAGATTTAACGCCGACTT GGCAAGCCGGAGCCGGGCACTCGAAAGAAAGCGGCGCTACCATTTTGACTCTGACGAAAGGGGTTCCGGA CACGAGCACAAGAGTTGTGTTCGAAAGAAGCCTTTCGAGTGTGGAGCTGAAATGCGGCAGGCCATGTCTA TGGGCAACTTGAATTCACCCAGCTTCTCCGAGAGCCAATCCATAGATTTTGGGGCTAACCCCTATGTGTG CGACGAATGTGGCCGGCAATTCTCAGTGATTTCCGAATTCGTAGAGCATCAAATTATGCACACCAGGGAG AACCTGTATGAATATGGTGAGAGTTTTATCCACTCAGTAGCAGTCAATGAAGTGCAGAAGGGCCAAGGAG GAGGCAAGCGCTTTGAATGTAAAGAGTGCGGGGAAACATTTTCTAGGAGTGCCGCCCTGGCAGAGCACCG CCAGATTCACGCTAGAGAGTACCTGGCCGAATGCAGGGACCAAGAAGACGAGGAGACTATTATGCCAAGC CCCACCTTCTCAGAGCTCCAAAAGATGTATGGGAAGGACAAGTTTTACGAGTGTAAGGTATGCAAGGAGA CGTTTCTTCACAGCTCAGCCCTCATCGAGCACCAAAAAATCCATGGCCGAGGCAACAGCGATGATAGAGA CAACGAGCGCGAGAGAGAGAGAGATAGGCTCCGCGCCAGAGCCCGCGAGCAGAGGGAGAGAGAAAGGGAG CGAGAAAGGGAGCGCGAGCTGGGGGAGCCCTTTCTGACGTGCCCAAACTTTAACGAGTTTAGGAAAATGT ATCGCAAAGACAAAATTTACGAATGCAAAGTCTGTGGAGAGTCCTTTCTGCATCTGAGCTCCCTCAGGGA ACATCAGAAAATCCACACCAGAGGAAATCCGTTCGAAAACAAATCACGCATGTGCGAGGAAACCTTTGTC CCGAGCCAATCCCTTCGGCGAAGACAAAAGACCTACCGGGAAAAGCTGTTTGACTTCAATAACGCAAGAG ATGCTCTGATGGGAAATTCTGATTCATCAGAACACCAGAAAAATCGCTCCCGCCGGAACTTTTTCGAGGG GCGAGGGTTCGAGAAGCCCTTTGTAGAAAGTCAGAAATCTCATACCATCACCCGCCCTCCTGAGAATAAG GACGACGACAAGCCGTTTACCATCAGCGTGAACCCAAATGATAAGCTTAAGTTTCCTATCATGGAGAACG GTTCCCAAGGAAAATCATATGAACGCAGTGTAATCCATTCTCTCGGTTCTGCAGAAGCACAGAAATCACA TGGTGGACTGGGCTTCTCAAAGCCAAGGCCGGTGGCGGAGAGTTCAACTCAGTCCAGCTCCAGCATCTAC TACCCCCGAGCCCATTCTGGAGGAAACACCTACGAAGGTAAGGAATACAAGGACAGTATAATACATAGTC TTCCTGCCCCTCGGCCACTTAAGCGGCATCGGGCTAACGACCATATTCAGTGCGATGAGGGCGGAGAGAG CAGCATATACATTCCCGACATCATTAACAAGGGACGCAAGATTCCCGCAAGAGAAGACGCCTATGAAGGA TCATCTTCCAGCAACTATCATACACCAAACGTGAGTCGCGCTGAACCACCTTCACTTAGTGGAGAGAGCC ATGATTCCAAGCAGGACGTGACATTCAGCGTCCCCTCATCATCAGTCAGAGAACATCAGAAAGCACGCGC GAAGAAAAAATACATAGAGCCGCGAAATAATGAGACATCCGTGATCCACTCTTTGCCATTTGGCGAACTG CTGGCTGGTCACAGGAGAGCCAAGTTCTTCGAGTGCCAGGAGTGCGGAGAAGCCTTCGCACGAAGGAGCG AGCTCATCGAACACCAGAAGATCCACGATCGCGAGCGGCCATCAGGGTCCCGACACTATGAACGATCAGT GATACGCAGCCTGGCCCCATCCGACCCTCAGACATCTTACGCACAGGAGAGGTTCATCCAGGAACAGGTT CGCAAATTTAGGGCCTTTGGCCAGCGGTCCACCACATCTAATAACCTGTCAGTGCAAAAAATTTATGCCC AGGAAACATTTAATGCAGAAGAGCCTCATGATAAAGAAACCCACGGACAAAAGATCCATGATAAAGAGCC ATACGGTAAAGAGCCCAGCGGCAAAGAGCCTCATGGTGATGAACCTCAGGACAAGGAGCCTCTTGATCAG GAGATGCGATCAGAGGAACCGCACGACGATAAACCTCACGGTCAGGAACCTCATGATGACAAGCCCCACG GCCAGGAGCCTCACGACGACAAGCCGCATGGTCAGGAGCCACACGGCGACGAACCTCACGGCCAGGAGCC TCACGGCGATGAACCACATGACAAGGAGCCTATTGATCAGGAAATGAGAAGTGAGGAGCCACATTCTGAG GAGAGCCACGGGGACGAGCCGCACGGAGAAGAATCACATGGCCAAGAGAAGGTTGAGGACGCTACGATTC AGGCTTCTGTGTCCGAGGAGCACCAAAAGGACGATGCAGGCGATGCGATTTACGAATGTCAGGACTGCGG TCTGGGATTTACCGACCTCAATGATCTCACGTCTCATCAGGACACCCATAGCAGGAAGGCTCTTGTAGAC AGCCGCGAGTACGCACACAGTGAAGTGCACGCGCACTCAGTGTCCGAGTTTGAAAAAAAGTGTAGTGGAG AAAAGCTGTATGAGTGCCCGAAGTGCGGCGAATCTTTTATACATTCATCCTTGCTCTTTGAGCACCAGCG AGTACACGAACAGGATCAGTTGTATTCTGTGAAGGCATGTGACGACGCATTCATAGCACTGCTCCCTGTG CGGCCTCGACGAAATTGCACTGTCGAGCGAAATCCGGCTGTGTCTGGTTCCGCAATACGCTGTAGACAGT GTGGTCAGGGATTCATCCACTCTTCTGCCTTGAACGAGCATATGCGGCAACACAGGGATAACGAGATCAT GGAGCAATCCGAGCTCTCAGACGAAATCTTTATTCAGGGGCTGGCCCTGACAGAATACCAAGGCAGCGAG ACAGAAGAGAAACTGTTTGAGTGCACCATCTGTGGAGAGTGTTTCTTCACCGCCAAGCAGCTCGGGGACC ATCACACCAAGGTACACAAGGACGAGCCTTATGAATACGGGCCCTCATATACCCATGCTTCCTTCCTTAC TGAACCGCTTAGGAAGCATATTCCTCTTTACGAGTGTAAGGACTGTGGCCAGAGCTTTTTGGATGATACC GTCATCGCTGAGAGAATGGTCTTTCATCCAGAACGGGAAGGCGGCTCAGAGATTGTCGCCGCTACAGCTC AGGAGGTGGAGGCTAACGTGTTGATCCCTCAGGAAGTCCTTCGCATCCAAGGTAGCAACGCAGAGGCGGC AGAACCAGAGGTGGAGGCCGCCGAGCCTGAGGTTGAAGCCGCCGAGCCCGAGGTGGAAGCTGCTGAGCCC AATGGTGAGGCGGAGGGACCTGACGGCGAGGCCGCCGAGCCTGATGGAGAGGCTGAACAGCCTAATGGCG AAGCTGAGCAACCGAACGGTGATGCTGATGAACCAGATGGCGCCGGTATCGAAGATCCTGAAGAACGGGC TGACGAGCCTGAAGAGGATGTGGAAGAGCCCGAGGGAGACGCCGACGAGCCAGACGGGGCAGATATCGAA GATCCAGAAGAGGAAGGCGAAGATCAGGAGATCGAGGTCGAGGAGCCTTACTACAATTGCCACGAGTGCG CTGAGACATTCGCCAGTTCTTCTGCATTTGGCGAGCATCTGAAGAGCCACGCCTCAGTGATCATCTTCGA GCCAGCGAACGCTCCTGGTGAGTGCTCCGGCTACATCGAAAGAGCTAGCACTTCCGCTGGGGGAGCAGAA CAGGCTGATGACAAGTACTTCAAGTGCGATGTCTGTGGCCAGCTGTTTAATGACAGGCTTTCTCTCGCTA GGCACCAGAACAGCCACACTGGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR224127 representing NM_008817 Red=Cloning site Green=Tags(s) MYHHEDDTNSDMNSDDDMSRSGRETPPPRPSHAFGSERDLERRGRSRDVEPRDRWPYTRNPRSRLPQRDL SLPVMSRPHFGLDRDDDRRSMDYESRSQDAESYQNVVELKEDKKPQNPIQDNLENYRKLLSLGVQLAEDD RHSHMTQGHSSRSKRTAYPSTSRGLKPMPEAKKPSHRRGICEDESSHGVIMEKFIKDVARNPKSGRAREL NERPPPRFPRPNDNWKDSSSSRRESVIQERGYEGSAFRGGFRFNADLASRSRALERKRRYHFDSDERGSG HEHKSCVRKKPFECGAEMRQAMSMGNLNSPSFSESQSIDFGANPYVCDECGRQFSVISEFVEHQIMHTRE NLYEYGESFIHSVAVNEVQKGQGGGKRFECKECGETFSRSAALAEHRQIHAREYLAECRDQEDEETIMPS PTFSELQKMYGKDKFYECKVCKETFLHSSALIEHQKIHGRGNSDDRDNERERERDRLRARAREQRERERE RERERELGEPFLTCPNFNEFRKMYRKDKIYECKVCGESFLHLSSLREHQKIHTRGNPFENKSRMCEETFV PSQSLRRRQKTYREKLFDFNNARDALMGNSDSSEHQKNRSRRNFFEGRGFEKPFVESQKSHTITRPPENK DDDKPFTISVNPNDKLKFPIMENGSQGKSYERSVIHSLGSAEAQKSHGGLGFSKPRPVAESSTQSSSSIY YPRAHSGGNTYEGKEYKDSIIHSLPAPRPLKRHRANDHIQCDEGGESSIYIPDIINKGRKIPAREDAYEG SSSSNYHTPNVSRAEPPSLSGESHDSKQDVTFSVPSSSVREHQKARAKKKYIEPRNNETSVIHSLPFGEL LAGHRRAKFFECQECGEAFARRSELIEHQKIHDRERPSGSRHYERSVIRSLAPSDPQTSYAQERFIQEQV RKFRAFGQRSTTSNNLSVQKIYAQETFNAEEPHDKETHGQKIHDKEPYGKEPSGKEPHGDEPQDKEPLDQ EMRSEEPHDDKPHGQEPHDDKPHGQEPHDDKPHGQEPHGDEPHGQEPHGDEPHDKEPIDQEMRSEEPHSE ESHGDEPHGEESHGQEKVEDATIQASVSEEHQKDDAGDAIYECQDCGLGFTDLNDLTSHQDTHSRKALVD SREYAHSEVHAHSVSEFEKKCSGEKLYECPKCGESFIHSSLLFEHQRVHEQDQLYSVKACDDAFIALLPV RPRRNCTVERNPAVSGSAIRCRQCGQGFIHSSALNEHMRQHRDNEIMEQSELSDEIFIQGLALTEYQGSE TEEKLFECTICGECFFTAKQLGDHHTKVHKDEPYEYGPSYTHASFLTEPLRKHIPLYECKDCGQSFLDDT VIAERMVFHPEREGGSEIVAATAQEVEANVLIPQEVLRIQGSNAEAAEPEVEAAEPEVEAAEPEVEAAEP NGEAEGPDGEAAEPDGEAEQPNGEAEQPNGDADEPDGAGIEDPEERADEPEEDVEEPEGDADEPDGADIE DPEEEGEDQEIEVEEPYYNCHECAETFASSSAFGEHLKSHASVIIFEPANAPGECSGYIERASTSAGGAE QADDKYFKCDVCGQLFNDRLSLARHQNSHTG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_008817
ORF Size	4713 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008817.2, NP_032843.2
RefSeq Size	6624 bp
RefSeq ORF	4716 bp
Locus ID	18616
UniProt ID	Q3URU2
MW	178.9 kDa
Gene Summary	Induces apoptosis in cooperation with SIAH1A. Acts as a mediator between p53/TP53 and BAX in a neuronal death pathway that is activated by DNA damage. Acts synergistically with TRAF2 and inhibits TNF induced apoptosis through activation of NF-kappa-B. Plays a role in regulating maternal behavior and offspring growth.[UniProtKB/Swiss-Prot Function]

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