Med13 (NM_001080931) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1110067M05Rik; D030023K18; Thrap1; Trap240
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR224038 representing NM_001080931 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTTCCTCCTTCGTGTCGAACGGGGCCAGCCTGGAAGATTGTCACTGTAACCTCTTCTGCCTGGCCG ACTTGACAGGAATTAAGTGGAAAAGATATGTATGGCAAGGCCCAACTTCCGCCCCTATTCTGTTTCCGGT GACAGAAGAAGACCCCATTTTGAGCAGTTTTAGTCGATGCCTTAAGGCAGATGTACTTGGTGTTTGGCGG CGAGATCAAAGACCTGGAAGAAGAGAATTGTGGATATTTTGGTGGGGTAAAGATCCCAATTTTGCTGACC TTATTCACCATGACTTATCAGAAGAGGAAGATGGAGTATGGGAGAATGGCCTTTCCTATGAATGCCGTAC TTTGCTTTTCAAAGCAGTTCACAATCTGTTGGAGCGATGTTTGATGAACCGAAACTTTGTACGAATTGGC AAGTGGTTTGTGAAGCCATATGAGAAAGATGAAAAACCTATAAATAAAAGTGAACACTTGTCCTGCTCTT TCACCTTTTTCTTGCATGGAGACAGCAATGTTTGTACCAGTGTGGAAATTAACCAACATCAACCTGTATA CCTTCTCAGTGAAGAGCATGTCACCCTTGCTCAACAGTCTAATAGCCCATTTCAAGTTATCTTAAGTCCA TTTGGACTAAATGGCACTCTCACGGGACAGGCTTTCAAGATGTCTGATTCAGCTACGAAAAAATTAATTG GTGAATGGAAACAGTTCTATCCTATTTCATGTGGTTTGAAGGAGATGTCTGAGGAGAAACAGGACGATAT GGATTGGGAAGACGATTCTTTGGCTGCGGTGGAAGTTCTTGTTGCGGGTGTCCGAATGATCTACCCAGCA TGCTTTGTGCTAGTCCCTCAGTCAGACATTCCTGCTCCTAGCTCTGTGGGAGCTTCTCACTGTTCAGCTT CTTGCTTGGGTATCCATCAAGTGCCTGCTTCCACAAGAGATCCTGCTATGTCTTCAGTTACTCTCACTCC ACCTACATCTCCTGAGGAAGTCCAAACAGTTGATCCTCAGTCCGCACAGAAGTGGGTCAAATTTTCTTCA GTATCCGATGGCTTCAGCACTGACAGTACTAGCCATCATGGTGGGAAAATACCCAGAAAATTAGCAAATC ATGTGGTGGATAGAGTCTGGCAAGAATGTAATATGAACAGATTACAGAACAAGAGGAAGTATTCTGCTAC ATCCAGCGGCCTGTGTGAGGAAGAGACTGCCGATAAAATAGGATGCTGGGACTTTGTTGAAGCCACACAA AGAACAAGCTGCAGTTGTTTGAGGCATAAAAGTCTCAAGACAAGAAATACTGGGCAACAAGGACAGGCAC CATCTTTAGGGCAGCAACAGCAAGTACTTCCTAAGCACAAGACCAATGAGAAGCAAGACAAGAGTGAGAA GCCACAGAAGCGCCCCTTGACTCCCTTTCACCATCGTGTATCAGTTAGTGATGAGATTGGCATGGACACA GATTCAGCCAGCCAAAGACTTGTGATCTCTGCTGCAGATAGTCAAGTGAGGTTTTCAAACATCCGAACTA ATGATGTAGCAAAGACTCCTCAGATGCATGGCACTGAACTGGCAAATTCACCTCAGCCTCCCCCACTTAG TCCTCATCCTTGTGATGTGGTTGATGAAGGAGTGACTAAAACACCTTCAACTCCGCAGAGTCAACATTTT TATCAAATGCCAACACCGGATCCCTTGGTTCCTACCAAACCAATGGAAGATAGGATAGACAGTTTGTCCC AGTCTTTCCCACCTCCATTTCAGGAAGCTGTAGAACCTACCGTGTATGTTGGTACAGCAGTAAGCTTGGA AGAAGATGAAGCTAATGTAGCCTGGAAGTATTACAAAGTCCCAAAGAAAAAAGATGTAGAATTTTTACCA CCTCAACTTCCAAATGATAAATTCAAGGATGATCCAGTTGGACCTTTTGGACAGGAAAGTGTAACATCAG TTACAGAGTTAATGGTGCAGTGTAAGAAGCCTTTAAAAGTTTCTGATGAAATAGTCCAGCAATATCAAAT TAAAAATCAGTATCTTTCAGCAATAGCATCTGATACAGAACAAGAACCTAAAATTGATCCATATGCATTT GTTGAAGGAGATGAGGAATTCATTTTTACTGATAAAAAAGATAGACAAAATAGCGAGAGAGAAGCTGGGA AGAAACACAAGGTGGAAGATGGGACATCTGCTGTCACAGTGTTATCACACGAAGAAGATGCTATGTCATT ATTTAGTCCCTCTAAACAAGATGCTCCACGCCCTACAAATCATGCCCGTCCTCCATCAACCAGTTTGATT TATGACTCAGATTTGGCTGTGTCTTACACTGACCTTGATAACCTCTTCAATTCTGATGAAGATGAATTAA CACCTGGATCTAAAAAGTCAGCAAGTGGATCAGATGATAAAGCCAGCTCCAAAGAATCAAAGACAGGAAA TCTGGATCCGTTGTCCTGCATAAGCACTGCAGACCTCCACAAAATGTACCCCACGCCACCCTCACTGGAA CAGCACATTATGGGCTTCTCTCCAATGAATATGAATAACAAAGAATACGGTAGTGTGGACACAGCACCCG GAGGGACTGTCCTGGAAGGAAATAGCTCTAGTGTAGGAACGCAGTTCAGAATCGAGGTCGAGGAAGGATT CTGTAGCCCTAAACCGTCTGAAATTAAAGATTTTTCTTATGTCTATAAGCCCGAAAATTGTCAAGTTCTA GTAGGATGTTCCATGTTTGCACCTTTAAAAACTCTACCAAGCCATTGTCTGCCTCCTATCAAATTGCCAG AAGAGTGTGTTTACCGGCAGAGCTGGACTGTTGGAAAATTGGACTTGCTTCCTTCAGGCCCTTCAATGCC ATTCATCAAGGAGGGTGATGGCAGTAATTTGGATCAGGACTATGGCCCCGCCTACACACCGCAAACCCAT GCTTCTTTTGGGATGCCTCCGAGCAGCGCACCTCCTAGTAATGGTGGTGCAGGAATTCTTCCTTCCCCAT CCACCCCTAGGTTTCCAACTCCAAGGACTCCAAGGACTCCAAGGACCCCTCGTGGGGCTGGTGGGCCTGC TAGTGCTCAAGGTTCAGTCAAGTATGAGAATTCAGACTTGTATTCACCAGCTTCCACCCCATCTACATGC CGACCCCTCAATTCTGTTGAACCTGCAACTGTTCCTTCCATCCCTGAAGCGCACAGTCTTTATGTAAACC TCATCCTTTCAGAATCAGTAATGAATTTGTTTAAAGACTGTAATTTTGATAGTTGCTGTATTTGTGTCTG CAATATGAATATCAAGGGTGCCGATGTTGGAGTTTACATTCCAGACCCAACCCAAGAAGCACAGTATCGG TGCACCTGTGGCTTCAGTGCTGTCATGAACCGAAAATTTGGAAATAATTCAGGATTATTTCTTGAAGATG AACTTGACATCATAGGACGTAACACAGACTGTGGGAAAGAAGCAGAAAAACGTTTTGAAGCTCTCAGGGC TTCCTCTGTGGAGAATGTTAATGGAGGCCTGAAGGAATCTGAAAAGGTGCCTGATGAATTAATATTACTG CTACAAGATCAATGCACTAATTTATTTTCACCCTTTGGAGCAGCAGACCAAGATCCTTTTCCCAAAGTGG GTATAAGTAGCAATTGGGTGCGTGTTGAGGAGCGGGACTGTTGTAGTGACTGCTGCCTCGCCTTAGAACA TGGGCGTCAGTTCATGGATAATATGTCAGGAGGGAAAGTTGATGAAGCACTTGTCAGAAGCTCATGCTTA CATCCGTGGGCCAAGCAAAATGATGCGAGCGTGCAGTGTTCACAGGATATACTTCGGATGCTCCTTTCTC TTCAGCCAGTTCTTCAAGATGCTATTCAGAAAAAAAGAACAGTGAGGCCTTGGGGTGTTCAAGGTCCTCT CACATGGCAACAATTTCATAAAATGGCTGGCCGAGGCTCTTATGGAACTGATGAATCTCCGGAACCACTG CCGATTCCCACGTTTTTGTTGGGTTATGATTACGATTTTCTGGTGCTTTCTCCATTTGCTCTCCCTTACT GGGAGAAACTTATGCTGGAACCCTATGGATCTCAAAGAGATATAGCCTATGTTGTACTCTGTCCAGAGAA TGAAGCTTTGTTAAATGGAGCCAGAAGCTTTTTTAGAGATCTCACTGCAATATATGAGTCCTGTCGATTA GGCCAACACAGACCTATTTCTCGATTGTTGACGGATGGGATCATGAAAGTGGGAGCTACTGCATCAAAGA AACTTTCAGAAAAGTTTGTAACAGAATGGTTTTCTCAGGCAGCTGATGGTAACAATGAAGCATTTTCCAA ATTGAAGCTGTATGCACAAGTCTGCAGATATGATTTAGGTCCATATCTTGCTTCTCAGCCGTTGGACAGC TCTCTGCTCTCCCAGCCAAATTTAGTTGCCCCTCCAAATCAGTCTTTGGTTACTGCACCTCAGATGACAA ATACTGGAAACGCTAATGCTCCATCTGCCACCTTAGCATCTGCGGCAAGTAGCACTATGACAATGACTTC AGGTGTTCCCATATCTACTTCAGTTGCCACAGCTAATTCAACTTTGACCACAACTTCATCTTCATCATCT TCCAGTCTGAGTAGTGGAGTATCATCAAATAAACTGCCTTCGTTTCCACCCTTTGGCAGTATGAACACTA GTGGTACAGGATCCATGTCTGCACAAGCAAGTACGGTTCAGAGTGGCCAGCTAGGAGGACAGCAGTCGTC ATCTCTGCAGGCAGCAGGAATATCAGGGGAGTCAGCTTCTCTTCCCACTCAGCCACATCCTGATGTGTCT GAAAGCACAATGGATCGTGATAAAGTGGGGATTCCCACAGATGGTGATTCACATGCAATTACCTACCCTC CTGCAATTGTTGTTTATATAATCGATCCTTTTACATATGAAAATAAAGACGAGAGCACTAACTCTTCTAA TGTATGGACTTTGGGGCTCCTTCGATGCTTCTTGGAAATGGTCCAAACTCTTCCTCCTCATATCAAGAGC ACTGTTTCTGTGCAGATTGTTCCTTGTCAGTACCTGTTACAACCTGTGAAACACGACGACAGACAGATCT ACTCCCAGCATTTAAAATCTTTGGCTTTTTCGGTGTTTACCCAGTGTCGGAGACCACTTCCAACATCAAC CAATGTAAAAACACTGACTGGGTTTGGTCCAGGCTTAGCTATGGAAACTGCTCTTAAAAGTCCTGATAGA CCAGAGTGTATTCGACTTTATACACCACCTTTTATTCTGGCTCCGGTGAAGGACAAACAAACAGAGCTAG GAGAGACCTTTGGAGAAGCTGGACAGAAATACAATGTTCTTTTTGTGGGCTACTGTTTATCACATGATCA AAGGTGGATTCTTGCATCTTGCACAGATCTATATGGAGAACTCCTAGAAACATGTATCATTAACATCGAT GTTCCAAATAGGGCCCGTCGGAAAAAGGGTTCTGCTAGAAGATTTGGTCTACAGAAACTGTGGGAGTGGT GCTTGGGACTTGTTCAGATGAGCTCTTTACCATGGAGAGTTGTGATTGGTCGCCTGGGAAGGATTGGACA TGGAGAGTTGAAAGATTGGAGTTGTTTGCTGAGTCGTCGAAACTTGCAATCTCTAAGCAAAAGGCTCAAA GACATGTGTAGGATGTGTGGCATATCTGCTGCAGACTCTCCAAGTATCCTTAGTGCTTGTTTGGTTGCAA TGGAACCCCAAGGTTCTTTTGTTATCATGCCAGATTCTGTGTCAACTGGTTCTGTTTTTGGAAGAAGTAC CACTCTAAATATGCAGACGCCTCAGCTAAATACCCCACAAGATACATCCTGTACTCATATACTTGTGTTC CCTACTTCTGCTTCTGTGCAAGTAGCTTCAGCTACTTACACCACTGAAAATTTGGATTTAGCTTTCAATC CCAACAATGATGGAGCAGACGGAATGGGTATCTTTGATTTATTAGACACAGGAGATGATCTTGATCCTGA TATCATTAATATCCTTCCTGCATCTCCAACTGCATCTCCTGTACATTCTCCAGGCTCTCATTACCCCCAT GGAGGTGATGCTGGCAAGGGTCAGGGTACTGATCGGCTACTTTCAACAGAATCGCATGATGAAGTAACTA ATATTCTTCAACAACCATTGGCCCTTGGATACTTTGTATCAACTGCCAAAGCGGGTCCATTACCTGACTG GTTCTGGTCAGCATGCCCTCAAGCACAATATCAATGCCCCCTTTTTCTTAAGGCCTCTTTGCACCTCCAC GTGCCTTCAGTGCAATCTGACGAGCTGCTTCACAGCAAACACTCCCACCCACTGGACTCAAATCAGACTT CAGATGTCCTCAGGTTTGTTTTGGAACAGTACAATGCACTCTCATGGCTAACCTGTGACCCTGCAGTCCA GGACAGACGCTCATGTCTCCCAGTTCATTTTGTGGTGCTGAATCAGTTATATAACTTCATCATGAATATG CTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR224038 representing NM_001080931 Red=Cloning site Green=Tags(s) MSSSFVSNGASLEDCHCNLFCLADLTGIKWKRYVWQGPTSAPILFPVTEEDPILSSFSRCLKADVLGVWR RDQRPGRRELWIFWWGKDPNFADLIHHDLSEEEDGVWENGLSYECRTLLFKAVHNLLERCLMNRNFVRIG KWFVKPYEKDEKPINKSEHLSCSFTFFLHGDSNVCTSVEINQHQPVYLLSEEHVTLAQQSNSPFQVILSP FGLNGTLTGQAFKMSDSATKKLIGEWKQFYPISCGLKEMSEEKQDDMDWEDDSLAAVEVLVAGVRMIYPA CFVLVPQSDIPAPSSVGASHCSASCLGIHQVPASTRDPAMSSVTLTPPTSPEEVQTVDPQSAQKWVKFSS VSDGFSTDSTSHHGGKIPRKLANHVVDRVWQECNMNRLQNKRKYSATSSGLCEEETADKIGCWDFVEATQ RTSCSCLRHKSLKTRNTGQQGQAPSLGQQQQVLPKHKTNEKQDKSEKPQKRPLTPFHHRVSVSDEIGMDT DSASQRLVISAADSQVRFSNIRTNDVAKTPQMHGTELANSPQPPPLSPHPCDVVDEGVTKTPSTPQSQHF YQMPTPDPLVPTKPMEDRIDSLSQSFPPPFQEAVEPTVYVGTAVSLEEDEANVAWKYYKVPKKKDVEFLP PQLPNDKFKDDPVGPFGQESVTSVTELMVQCKKPLKVSDEIVQQYQIKNQYLSAIASDTEQEPKIDPYAF VEGDEEFIFTDKKDRQNSEREAGKKHKVEDGTSAVTVLSHEEDAMSLFSPSKQDAPRPTNHARPPSTSLI YDSDLAVSYTDLDNLFNSDEDELTPGSKKSASGSDDKASSKESKTGNLDPLSCISTADLHKMYPTPPSLE QHIMGFSPMNMNNKEYGSVDTAPGGTVLEGNSSSVGTQFRIEVEEGFCSPKPSEIKDFSYVYKPENCQVL VGCSMFAPLKTLPSHCLPPIKLPEECVYRQSWTVGKLDLLPSGPSMPFIKEGDGSNLDQDYGPAYTPQTH ASFGMPPSSAPPSNGGAGILPSPSTPRFPTPRTPRTPRTPRGAGGPASAQGSVKYENSDLYSPASTPSTC RPLNSVEPATVPSIPEAHSLYVNLILSESVMNLFKDCNFDSCCICVCNMNIKGADVGVYIPDPTQEAQYR CTCGFSAVMNRKFGNNSGLFLEDELDIIGRNTDCGKEAEKRFEALRASSVENVNGGLKESEKVPDELILL LQDQCTNLFSPFGAADQDPFPKVGISSNWVRVEERDCCSDCCLALEHGRQFMDNMSGGKVDEALVRSSCL HPWAKQNDASVQCSQDILRMLLSLQPVLQDAIQKKRTVRPWGVQGPLTWQQFHKMAGRGSYGTDESPEPL PIPTFLLGYDYDFLVLSPFALPYWEKLMLEPYGSQRDIAYVVLCPENEALLNGARSFFRDLTAIYESCRL GQHRPISRLLTDGIMKVGATASKKLSEKFVTEWFSQAADGNNEAFSKLKLYAQVCRYDLGPYLASQPLDS SLLSQPNLVAPPNQSLVTAPQMTNTGNANAPSATLASAASSTMTMTSGVPISTSVATANSTLTTTSSSSS SSLSSGVSSNKLPSFPPFGSMNTSGTGSMSAQASTVQSGQLGGQQSSSLQAAGISGESASLPTQPHPDVS ESTMDRDKVGIPTDGDSHAITYPPAIVVYIIDPFTYENKDESTNSSNVWTLGLLRCFLEMVQTLPPHIKS TVSVQIVPCQYLLQPVKHDDRQIYSQHLKSLAFSVFTQCRRPLPTSTNVKTLTGFGPGLAMETALKSPDR PECIRLYTPPFILAPVKDKQTELGETFGEAGQKYNVLFVGYCLSHDQRWILASCTDLYGELLETCIINID VPNRARRKKGSARRFGLQKLWEWCLGLVQMSSLPWRVVIGRLGRIGHGELKDWSCLLSRRNLQSLSKRLK DMCRMCGISAADSPSILSACLVAMEPQGSFVIMPDSVSTGSVFGRSTTLNMQTPQLNTPQDTSCTHILVF PTSASVQVASATYTTENLDLAFNPNNDGADGMGIFDLLDTGDDLDPDIINILPASPTASPVHSPGSHYPH GGDAGKGQGTDRLLSTESHDEVTNILQQPLALGYFVSTAKAGPLPDWFWSACPQAQYQCPLFLKASLHLH VPSVQSDELLHSKHSHPLDSNQTSDVLRFVLEQYNALSWLTCDPAVQDRRSCLPVHFVVLNQLYNFIMNM L TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001080931
ORF Size	6513 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001080931.2
RefSeq Size	11787 bp
RefSeq ORF	6516 bp
Locus ID	327987
UniProt ID	Q5SWW4
MW	239 kDa
Gene Summary	Component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors (By similarity).[UniProtKB/Swiss-Prot Function]

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