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Klra6 (NM_008464) Mouse Tagged ORF Clone
CAT＃: MR224004
- TrueORF®
Klra6 (Myc-DDK-tagged) - Mouse killer cell lectin-like receptor, subfamily A, member 6 (Klra6)
ORF Plasmid: tGFP
"NM_008464" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ly-49f; Ly49; Ly49f |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224004 representing NM_008464
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTGAGCCGGAGGTCACTTACTCAACTGTGAGACTTCATAAGTCTTCAAGGTTGCAGAAACTTGTAA GGCATGAGGAGACTCAAGGGCCCAGAGAAGCTGGCTACAGAAAGTGTTCAGTATGCTGGCAGCTCATTGT GAAAGCTCTTGGAATCCTCTGTTTCCTTCTTCTTATAACAGTTGCAGTGTTGGCAGTAAAGATTTTTCAG TATGGTCAACACAACCAAGAAATCCATGAAACTCTAAACTACCACCATAACTGCAGCAACATGCAAAGTG ATTTCAACTTAAAGGAAGAAATGTTGACAAATAGGTCTATAGACTCTAGGCCAGGCAATGAACTTCTGGA ATCCCTCAACAGAGAACAGAACAGAGGGTACAGTGAAACTAAGACAGATTTAGATTCCTCACAGGACACA GGGACAGGTGTTAAATACTGGTTCTGCTACCGTACTAAATGTTATTATTTCATCATGAACAAAAATACAT GGAGTGGATGTAAACAGAACTGCCAGCATTACAGCTTACCCCTTGTGAAGATAGACGATGAAAATGAACT GAAATTCCTTCAATTCCAGGTTATTCCAGACAGTTACTGGATTGGATTGTCATATGATAAGGAAAAAAAG GAGTGGGCATGGATTGACAATGGCCAATCTAAACTTGACATGAAAATAAGGAAAATGAACTTTAAGCCTG GAGGATGTGTATTTTTATCTAAAAGAAGACTAGAAGATACTAACTGTAAAAATTCCCACTACTGTATTTG TGGGAAGAAACTGGATAAATTTCCTCAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224004 representing NM_008464
Red=Cloning site Green=Tags(s) MSEPEVTYSTVRLHKSSRLQKLVRHEETQGPREAGYRKCSVCWQLIVKALGILCFLLLITVAVLAVKIFQ YGQHNQEIHETLNYHHNCSNMQSDFNLKEEMLTNRSIDSRPGNELLESLNREQNRGYSETKTDLDSSQDT GTGVKYWFCYRTKCYYFIMNKNTWSGCKQNCQHYSLPLVKIDDENELKFLQFQVIPDSYWIGLSYDKEKK EWAWIDNGQSKLDMKIRKMNFKPGGCVFLSKRRLEDTNCKNSHYCICGKKLDKFPH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008464 |
| ORF Size | 798 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008464.2, NP_032490.1 |
| RefSeq Size | 1116 bp |
| RefSeq ORF | 801 bp |
| Locus ID | 16637 |
| UniProt ID | Q60653 |
| MW | 31.3 kDa |
| Gene Summary | This gene belongs to the highly polymorphic family of C-type lectin-like Ly49 genes that are expressed in natural killer (NK) cells. The encoded protein is a homodimeric type II transmembrane receptor located at the cell surface and inhibits NK cell activation upon ligand binding. This gene is located in a cluster of several Klra paralogs on chromosome 6. Different strains of mice show variation in the number of paralogs, including strain specific duplications, deletions and pseudogene sequences. [provided by RefSeq, Apr 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC208837 | Klra6 (untagged) - Mouse killer cell lectin-like receptor, subfamily A, member 6 (Klra6), (10ug) |
CNY 3,990.00 |
|
| MG224004 | Klra6 (tGFP-tagged) - Mouse killer cell lectin-like receptor subfamily A member 6 (Klra6), (10ug) |
CNY 4,000.00 |
|
| MR224004L3 | Lenti ORF clone of Klra6 (Myc-DDK-tagged) - Mouse killer cell lectin-like receptor, subfamily A, member 6 (Klra6) |
CNY 4,750.00 |
|
| MR224004L4 | Lenti ORF clone of Klra6 (mGFP-tagged) - Mouse killer cell lectin-like receptor, subfamily A, member 6 (Klra6) |
CNY 4,750.00 |
