Myt1l (NM_008666) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900046C06Rik; 2900093J19Rik; C630034G21Rik; mKIAA1106; Nztf1; Pmng1; Png-1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR223825 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACGTGGACTCTGAGGAGAAGCGCCATCGCACACGGTCCAAAGGGGTTCGAGTTCCTGTGGAGCCAG CCATACAAGAGCTGTTCAGCTGTCCCACTCCAGGCTGCGACGGCAGTGGTCACGTCAGTGGCAAATATGC ACGACACAGAAGTGTATATGGTTGTCCCTTGGCTAAAAAAAGAAAAACGCAAGATAAACAGCCCCAAGAA CCTGCTCCCAAGCGAAAACCATTTGCAGTAAAAGCAGATAGTTCCTCAGTAGACGAATGTTATGAGAGTG ATGGTACTGAAGACATGGATGATAAGGAGGAAGATGATGATGAGGAGTTCTCTGAAGACAATGATGAGCA AGGGGATGATGACGACGAAGATGAGGTGGATCGGGAAGACGAGGAGGAGATCGAGGAGGAAGATGATGAA GAAGATGATGATGATGAAGATGGTGACGATGTAGAAGAGGAAGAAGAGGATGATGATGAAGAGGAGGAAG AAGAGGAAGAGGAAGAAGAAAATGAAGACCATCAAATGAGTTGTACTCGAATAATGCAGGACACAGACAA GGATGATAACAACAATGATGAGTATGATAACTATGATGAACTGGTAGCTAAGTCGCTATTAAATCTTGGC AAAATTGCTGAGGATGCAGCATACCGAGCCAGGACTGAATCAGAGATGAACAGCAATACCTCCAATAGTC TGGAGGACGATAGTGACAAAAACGAAAACCTCGGTCGGAAAAGCGAACTGAGTCTAGACTTAGACAGTGA TGTTGTTAGAGAAACAGTGGACTCCCTTAAGCTGTTAGCACAAGGACATGGTGTTGTGCTATCAGAGAAT ATCAGTGACAGAAGTTATGCTGAGGGGATGTCACAGCAGGACAGTAGAAATATGAACTATGTCATGCTAG GGAAGCCCATGAACAATGGACTCATGGAGAAGATGGTGGAGGAGAGTGATGAGGAAGTGTGTCTAAGTAG TCTAGAGTGCCTGAGGAACCAGTGCTTTGACCTGGCCAGGAAACTCAGCGAGACCAACCCACAGGACAGG AGTCAGCCACCCAACATGAGTGTGCGCCAACATGTCCGGCAAGAGGACGACTTCCCTGGGAGGACGCCAG ACAGGAGCTACTCGGATATGATGAACCTTATGCGGCTGGAGGAGCAGCTCAGTCCCAGGTCTAGAACGTT CTCCAGCTGTGCCAAGGAGGATGGGTGTCATGAGAGGGATGATGACACCACCTCAGTGAACTCAGACAGG TCTGAGGAAGTGTTTGACATGACCAAGGGCAACCTGACTCTGCTAGAGAAAGCCATTGCCTTGGAGACAG AGAGAGCCAAGGCCATGCGGGAGAAGATGGCCATGGATGCTGGGAGAAGGGATAACCTGAGATCCTATGA GGACCAGTCTCCAAGACAGCTGGCTGGGGAAGACAGAAAATCCAAATCCAGTGACAGCCATGTCAAAAAG CCATACTATGATCCCTCAAGAACAGAAAAGAGAGAGAGCAAGTGTCCAACCCCCGGGTGTGATGGAACCG GCCACGTAACTGGGCTTTACCCGCATCACCGCAGTCTGTCTGGATGCCCGCACAAAGATAGGGTCCCTCC AGAAATTCTTGCCATGCATGAAAATGTTCTCAAGTGTCCCACTCCAGGCTGCACAGGGCGAGGGCATGTG AATAGCAACAGGAACTCGCACAGAAGCCTCTCTGGATGCCCCATTGCTGCTGCAGAAAAACTGGCAAAGG CCCAAGAGAAACACCAGAGCTGTGATGTGTCCAAATCCAACCAGGCCTCAGACCGAGTCCTCAGGCCAAT GTGCTTTGTCAAACAGCTTGAGATTCCTCAGTATGGCTACAGAAACAATGTTCCCACAACCACACCACGC TCCAACCTGGCCAAGGAGCTTGAGAAATACTCCAAGACTTCGTTTGAGTACAACAGTTACGACAACCATA CTTATGGCAAAAGAGCCATAGCTCCCAAGGTGCAAACCAGGGACATATCCCCCAAAGGATATGACGATGC CAAGCGGTACTGCAAGAATGCCAGCCCCAGCAGCAGCACCACCAGCAGCTATGCACCTAGCAGCAGCAGC AACCTCAGCTGTGGTGGTGGCAGCAGCGCCAGTAGCACGTGTAGCAAGAGCAGCTTTGACTACACACATG ACATGGAGGCCGCACACATGGCAGCCACAGCCATTCTCAACCTGTCCACACGTTGTCGTGAAATGCCACA GAACCTGTCCACCAAGCCACAGGACCTGTGTACTGCCCGGAACCCAGACATGGAGGTGGATGAGAATGGC ACCCTGGACCTGAGCATGAACAAGCAGAGGCCTCGAGACAGCTGCTGCCCAGTCCTGACACCCCTGGAAC CCATGTCTCCGCAGCAGCAGGCCGTGATGAGCAGCCGATGCTTCCAGCTGAGCGAGGGGGATTGCTGGGA CTTGCCTGTAGACTACACCAAAATGAAGCCTCGGAGGGTAGATGAGGATGAGCCCAAAGAGATTACCCCA GAAGACTTGGACCCATTCCAGGAGGCTCTGGAAGAAAGACGGTATCCAGGGGAGGTGACCATCCCAAGCC CCAAACCCAAGTACCCTCAGTGCAAGGAAAGCAAAAAGGACTTAATAACTCTGTCTGGCTGCCCCCTGGC GGACAAAAGCATTCGAAGTATGCTGGCCACCAGTTCCCAAGAGCTCAAGTGCCCCACCCCTGGCTGTGAC GGTTCTGGACACATCACTGGCAATTACGCTTCTCATCGAAGCCTTTCTGGGTGCCCGAGAGCAAAGAAGA GTGGCATCCGGATAGCACAGAGCAAAGAGGACAAGGAAGACCAGGAGCCAATCAGGTGTCCGGTACCTGG CTGTGACGGTCAGGGACACATCACTGGGAAGTATGCATCCCACCGCAGCGCCTCCGGGTGTCCCTTGGCA GCCAAGAGGCAGAAAGATGGGTACCTTAATGGCTCCCAGTTCTCCTGGAAGTCGGTCAAGACGGAGGGCA TGTCCTGCCCTACCCCCGGGTGTGATGGGTCAGGACACGTCAGTGGCAGCTTCCTCACACACCGCAGCTT GTCAGGATGTCCAAGAGCCACATCAGCAATGAAGAAAGCAAAGCTGTCTGGAGAACAGATGTTGACTATC AAGCAGCGAGCCAGCAACGGTATAGAAAATGATGAAGAAATCAAGCAGTTAGATGAAGAGATCAAGGAGC TTAATGAGTCCAATTCCCAGATGGAGGCTGACATGATCAAACTCAGAACTCAGATCACCACAATGGAGAG CAACCTGAAGACGATTGAGGAGGAGAACAAAGTCATTGAACAGCAGAATGAGTCGCTCTTGCACGAGTTG GCCAACCTGAGCCAGTCCCTGATCCACAGCCTCGCCAACATCCAGCTGCCTCACATGGATCCAATCAATG AACAAAATTTTGATGCTTACGTGACTACTTTGACGGAAATGTATACAAATCAAGATCGTTATCAGAGTCC AGAAAATAAAGCCCTACTGGAAAATATAAAGCAGGCTGTGAGAGGAATTCAGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR223825 protein sequence Red=Cloning site Green=Tags(s) MDVDSEEKRHRTRSKGVRVPVEPAIQELFSCPTPGCDGSGHVSGKYARHRSVYGCPLAKKRKTQDKQPQE PAPKRKPFAVKADSSSVDECYESDGTEDMDDKEEDDDEEFSEDNDEQGDDDDEDEVDREDEEEIEEEDDE EDDDDEDGDDVEEEEEDDDEEEEEEEEEEENEDHQMSCTRIMQDTDKDDNNNDEYDNYDELVAKSLLNLG KIAEDAAYRARTESEMNSNTSNSLEDDSDKNENLGRKSELSLDLDSDVVRETVDSLKLLAQGHGVVLSEN ISDRSYAEGMSQQDSRNMNYVMLGKPMNNGLMEKMVEESDEEVCLSSLECLRNQCFDLARKLSETNPQDR SQPPNMSVRQHVRQEDDFPGRTPDRSYSDMMNLMRLEEQLSPRSRTFSSCAKEDGCHERDDDTTSVNSDR SEEVFDMTKGNLTLLEKAIALETERAKAMREKMAMDAGRRDNLRSYEDQSPRQLAGEDRKSKSSDSHVKK PYYDPSRTEKRESKCPTPGCDGTGHVTGLYPHHRSLSGCPHKDRVPPEILAMHENVLKCPTPGCTGRGHV NSNRNSHRSLSGCPIAAAEKLAKAQEKHQSCDVSKSNQASDRVLRPMCFVKQLEIPQYGYRNNVPTTTPR SNLAKELEKYSKTSFEYNSYDNHTYGKRAIAPKVQTRDISPKGYDDAKRYCKNASPSSSTTSSYAPSSSS NLSCGGGSSASSTCSKSSFDYTHDMEAAHMAATAILNLSTRCREMPQNLSTKPQDLCTARNPDMEVDENG TLDLSMNKQRPRDSCCPVLTPLEPMSPQQQAVMSSRCFQLSEGDCWDLPVDYTKMKPRRVDEDEPKEITP EDLDPFQEALEERRYPGEVTIPSPKPKYPQCKESKKDLITLSGCPLADKSIRSMLATSSQELKCPTPGCD GSGHITGNYASHRSLSGCPRAKKSGIRIAQSKEDKEDQEPIRCPVPGCDGQGHITGKYASHRSASGCPLA AKRQKDGYLNGSQFSWKSVKTEGMSCPTPGCDGSGHVSGSFLTHRSLSGCPRATSAMKKAKLSGEQMLTI KQRASNGIENDEEIKQLDEEIKELNESNSQMEADMIKLRTQITTMESNLKTIEEENKVIEQQNESLLHEL ANLSQSLIHSLANIQLPHMDPINEQNFDAYVTTLTEMYTNQDRYQSPENKALLENIKQAVRGIQV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_008666
ORF Size	3555 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_008666.3, NP_032692.2
RefSeq Size	7192 bp
RefSeq ORF	3558 bp
Locus ID	17933
MW	132.8 kDa
Gene Summary	Transcription factor that plays a key role in neuronal differentiation by specifically repressing expression of non-neuronal genes during neuron differentiation (PubMed:28379941). In contrast to other transcription repressors that inhibit specific lineages, mediates repression of multiple differentiation programs (PubMed:28379941). Also represses expression of negative regulators of neurogenesis, such as members of the Notch signaling pathway, including HES1 (PubMed:28379941). The combination of three transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to reprogram fibroblasts and other somatic cells into induced neuronal (iN) cells in vitro (PubMed:20107439, PubMed:24243019, PubMed:27281220). Directly binds the 5'-AAGTT-3' core motif present on the promoter of target genes and represses transcription by recruiting a multiprotein complex containing SIN3B (PubMed:28379941). The 5'-AAGTT-3' core motif is absent from the promoter of neural genes (PubMed:28379941).[UniProtKB/Swiss-Prot Function]

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