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Zfand5 (NM_009551) Mouse Tagged ORF Clone
CAT＃: MR223781
- TrueORF®
Zfand5 (Myc-DDK-tagged) - Mouse zinc finger, AN1-type domain 5 (Zfand5)
ORF Plasmid: tGFP
"NM_009551" in other vectors (4)
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CNY 2,400.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2310057A04Rik; 5830475F03Rik; AA415485; Za20d2; Zfp216 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223781 representing NM_009551
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCAGGAGACTAACCAGACCCCAGGGCCCATGCTGTGTAGTACAGGATGTGGCTTTTATGGGAATC CTAGGACAAATGGAATGTGTTCTGTTTGCTACAAAGAACATCTTCAGAGACAGCAGAATAGTGGCAGAAT GAGCCCAATGGGGACAGCTAGTGGTTCCAACAGTCCTACCTCAGATTCTGCATCTGTACAGAGAGCAGAT GCTGGTTTAAACAACTGTGAAGGTGCTGCTGGCAGCACATCTGAAAAATCAAGAAATGTGCCTGTGGCTG CCTTGCCTGTAACTCAACAAATGACAGAAATGAGCATTTCAAGAGAGGACAAAATAACTACCCCGAAAAC AGAGGTGTCAGAGCCAGTTGTCACTCAGCCCAGTCCATCAGTTTCTCAGCCCAGTTCTTCTCAAAGTGAA GAAAAAGCTCCTGAGTTGCCCAAACCAAAGAAGAACAGATGTTTTATGTGTAGAAAGAAAGTTGGCCTTA CAGGGTTTGACTGCCGATGTGGAAATTTGTTTTGTGGACTTCACCGTTACTCTGACAAGCACAACTGTCC ATATGATTACAAAGCAGAAGCTGCAGCAAAAATCAGAAAAGAAAATCCAGTTGTTGTGGCTGAAAAAATC CAGAGAATA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223781 representing NM_009551
Red=Cloning site Green=Tags(s) MAQETNQTPGPMLCSTGCGFYGNPRTNGMCSVCYKEHLQRQQNSGRMSPMGTASGSNSPTSDSASVQRAD AGLNNCEGAAGSTSEKSRNVPVAALPVTQQMTEMSISREDKITTPKTEVSEPVVTQPSPSVSQPSSSQSE EKAPELPKPKKNRCFMCRKKVGLTGFDCRCGNLFCGLHRYSDKHNCPYDYKAEAAAKIRKENPVVVAEKI QRI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009551 |
| ORF Size | 639 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009551.5, NP_033577.1 |
| RefSeq Size | 7385 bp |
| RefSeq ORF | 642 bp |
| Locus ID | 22682 |
| UniProt ID | O88878 |
| MW | 23.1 kDa |
| Gene Summary | Involved in protein degradation via the ubiquitin-proteasome system. May act by anchoring ubiquitinated proteins to the proteasome. Plays a role in ubiquitin-mediated protein degradation during muscle atrophy. Plays a role in the regulation of NF-kappa-B activation and apoptosis. Inhibits NF-kappa-B activation triggered by overexpression of RIPK1 and TRAF6 but not of RELA. Inhibits also tumor necrosis factor (TNF), IL-1 and TLR4-induced NF-kappa-B activation in a dose-dependent manner. Overexpression sensitizes cells to TNF-induced apoptosis. Is a potent inhibitory factor for osteoclast differentiation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209663 | Zfand5 (untagged) - Mouse zinc finger, AN1-type domain 5 (Zfand5), (10ug) |
CNY 3,990.00 |
|
| MG223781 | Zfand5 (tGFP-tagged) - Mouse zinc finger AN1-type domain 5 (Zfand5), (10ug) |
CNY 2,850.00 |
|
| MR223781L3 | Lenti ORF clone of Zfand5 (Myc-DDK-tagged) - Mouse zinc finger, AN1-type domain 5 (Zfand5) |
CNY 4,750.00 |
|
| MR223781L4 | Lenti ORF clone of Zfand5 (mGFP-tagged) - Mouse zinc finger, AN1-type domain 5 (Zfand5) |
CNY 4,750.00 |
