United States
Germany
Japan
United Kingdom
China
Pou2f1 (NM_011137) Mouse Tagged ORF Clone
CAT＃: MR223763
- TrueORF®
Pou2f1 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 1
ORF Plasmid: tGFP
"NM_011137" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 8,720.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810482H01Rik; NF-A1; Oct-1; Oct1; Otf-1; Otf1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223763 representing NM_011137
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGACGGAGGAGCAGCGAGTCAAGATGAGAGTTCAGCCGCGGCGGCAGCAGCAGCAGACTCAAGAA TGAATAATCCATCAGAAACCAATAAATCATCTATGGAGAGTGAAGATGCCAGCACAGGCACACAAACCAA TGGTCTGGACTTTCAGAAACAGCCCGTGCCCGTTGGAGGAGCGATCTCCACAGCCCAGGCCCAGGCCTTC CTCGGACATCTTCACCAGGTCCAGCTAGCTGGGACAAGTTTACAGGCTGCTGCTCAGTCTTTAAATGTAC AGTCTAAATCCAGTGAAGAGTCGGGAGATTCGCAGCAGTCGAGCCAGCCTTCTTCCCAGCCGCCTTCAGT GCAGTCAGCCATTCCCCAGACCCAGCTAATGCTGGCTGGGGGACAGATAACTGGGCTCACGTTGACCCCA GCCCAGCAACAGTTACTGCTACAGCAGGCGCAGGCCCAGGCCCAGCTCCTGGCCGCTGCAGTGCAGCAAC ACTCCGCCAGCCAACAGCACAGTGCTGCTGGGGCCACCATCTCAGCCTCCGCCGCCACACCCATGACGCA GATCCCCCTGTCTCAGCCCATACAGATTGCACAGGATCTTCAACAATTGCAACAGCTTCAGCAGCAAAAT CTCAACTTGCAACAGTTTGTGTTGGTGCACCCAACCACCAACCTGCAACCAGCACAGTTTATCATCTCAC AGACCCCCCAGGGCCAGCAGGGTCTCCTGCAAGCGCAAAATCTTTTAACGCAACTACCTCAGCAAAGCCA AGCCAACCTCCTACAGCCACAGCCAAGCATCACCCTCACGTCCCAGCCTACCACCCCAACTCGCACAATA GCAGCAGCCTCAGTTCAGACACTTCCACAGAGCCAGTCAACACCAAAGCGAATTGACACTCCCAGCTTGG AGGAGCCCAGTGACCTTGAGGAGCTTGAGCAGTTTGCCAAGACTTTCAAACAAAGACGAATCAAACTTGG ATTCACTCAGGGTGATGTTGGGCTCGCTATGGGGAAATTATATGGAAATGACTTCAGCCAAACCACCATC TCTCGCTTTGAAGCCTTGAACCTCAGCTTTAAGAACATGTGCAAGTTAAAGCCCCTTTTAGAGAAGTGGC TAAATGATGCAGAGAACCTCTCATCTGATTCTACAGCATCTAGCCCAAGTGCTTTGAATTCTCCAGGATT GGGGGCTGAGGGCTTGAATCGTAGGAGGAAAAAACGCACCAGCATAGAGACCAACATCCGTGTGGCCTTA GAGAAGAGTTTCATGGAGAATCAAAAGCCTACCTCGGAAGACATCACCTTGATTGCTGAACAGCTCAATA TGGAAAAGGAGGTGATTCGTGTTTGGTTTTGTAACCGCCGCCAGAAGGAGAAAAGAATCAACCCGCCCAG CAGTGGTGGGACCAGCAGCTCACCTATCAAAGCAATTTTCCCCAGCCCAGCCTCATTGGTGGCAACCACT CCAAGCCTTGTGACAAGCAGTACGGCAACTACCCTCACAGTCAACCCTGTCCTCCCTTTAACCAGTGCTG CTGTGACTAATCTCTCTCTTACAGATCAAGATCTTAGAAGAGGATGCAGCTGGGAAGTGCTTAGGAGTCT ACCAGACAGAGTCACCACCACAGCAGGCACTACAGACTCGACGTCCAACAACAACACGGCCACGGTGATT TCCACAGCACCCCCTGCTTCCTCAGCAGTCACATCCCCTTCCTTGAGTCCCTCTCCCTCTGCCTCGGCCT CCACCTCAGAGGCCTCCAGTGCCAGTGAGACCAACACGACACAGACCACCTCCACGCCTCTTCCCTCCCC TCTCGGAGCCAGCCAGGTGATGGTGACCACGCCCGGCTTACAGACAGCAGCCGCCGCTCTCCAAGGAGCG GCACAGTTGCCAGCAAACGCCAGTCTTGCTGCTATGGCTGCTGCTGCGGGACTCAGCCCAGGCCTCATGG CACCCTCACAGTTTGCTGCTGGAGGTGCCTTACTCAGTCTCAGTCCGGGGACTCTGGGCAGTGCTCTCAG CCCAGCCCTAATGAGCAACAGTACACTGGCAACGATTCAAGCTCTTGCTTCTAGTGGCTCTCTTCCAATA ACGTCTCTGGATGCAACTGGGAACCTGGTATTTGCCAATGCAGGAGGAGCCCCGAACATCGTGACTGCAC CTCTGTTCCTGAACCCTCAGAACCTCTCTCTGCTCACCAGCAACCCAGTAAGCTTGGTTTCTGCCGCTGC AGCCTCCACAGGGAACTCTGCACCTACAGCCAGCCTTCATGCCTCCTCCACCTCAACTGAGTCCATCCAG AGCTCTCTGTTCACAGTCGCCTCTGCCAGTGGGCCTGCTTCCACCACCACAGCTGCCTCCAAGGCACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223763 representing NM_011137
Red=Cloning site Green=Tags(s) MADGGAASQDESSAAAAAAADSRMNNPSETNKSSMESEDASTGTQTNGLDFQKQPVPVGGAISTAQAQAF LGHLHQVQLAGTSLQAAAQSLNVQSKSSEESGDSQQSSQPSSQPPSVQSAIPQTQLMLAGGQITGLTLTP AQQQLLLQQAQAQAQLLAAAVQQHSASQQHSAAGATISASAATPMTQIPLSQPIQIAQDLQQLQQLQQQN LNLQQFVLVHPTTNLQPAQFIISQTPQGQQGLLQAQNLLTQLPQQSQANLLQPQPSITLTSQPTTPTRTI AAASVQTLPQSQSTPKRIDTPSLEEPSDLEELEQFAKTFKQRRIKLGFTQGDVGLAMGKLYGNDFSQTTI SRFEALNLSFKNMCKLKPLLEKWLNDAENLSSDSTASSPSALNSPGLGAEGLNRRRKKRTSIETNIRVAL EKSFMENQKPTSEDITLIAEQLNMEKEVIRVWFCNRRQKEKRINPPSSGGTSSSPIKAIFPSPASLVATT PSLVTSSTATTLTVNPVLPLTSAAVTNLSLTDQDLRRGCSWEVLRSLPDRVTTTAGTTDSTSNNNTATVI STAPPASSAVTSPSLSPSPSASASTSEASSASETNTTQTTSTPLPSPLGASQVMVTTPGLQTAAAALQGA AQLPANASLAAMAAAAGLSPGLMAPSQFAAGGALLSLSPGTLGSALSPALMSNSTLATIQALASSGSLPI TSLDATGNLVFANAGGAPNIVTAPLFLNPQNLSLLTSNPVSLVSAAAASTGNSAPTASLHASSTSTESIQ SSLFTVASASGPASTTTAASKAQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011137 |
| ORF Size | 2382 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011137.3, NP_035267.2 |
| RefSeq Size | 12978 bp |
| RefSeq ORF | 2382 bp |
| Locus ID | 18986 |
| UniProt ID | P25425 |
| MW | 81.6 kDa |
| Gene Summary | Transcription factor that binds to the octamer motif (5'-ATTTGCAT-3') and activates the promoters of the genes for some small nuclear RNAs (snRNA) and of genes such as those for histone H2B and immunoglobulins. Modulates transcription transactivation by NR3C1, AR and PGR (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Transcriptional co-repressor CtBP2 orchestrates epithelial-mesenchymal transition through a novel transcriptional holocomplex with OCT1
,Ma, Y;Sekiya, M;Kainoh, K;Matsuda, T;Iwasaki, H;Osaki, Y;Sugano, Y;Suzuki, H;Takeuchi, Y;Miyamoto, T;Yahagi, N;Nakagawa, Y;Matsuzaka, T;Shimano, H;,
Biochem. Biophys. Res. Commun.
,PubMed ID 31866012
[POU2F1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC221631 | Pou2f1 (untagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 1, (10ug) |
CNY 10,170.00 |
|
| MG223763 | Pou2f1 (tGFP-tagged) - Mouse POU domain class 2 transcription factor 1 (Pou2f1) transcript variant 1, (10ug) |
CNY 8,650.00 |
|
| MR223763L3 | Lenti ORF clone of Pou2f1 (Myc-DDK-tagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 1 |
CNY 9,790.00 |
|
| MR223763L4 | Lenti ORF clone of Pou2f1 (mGFP-tagged) - Mouse POU domain, class 2, transcription factor 1 (Pou2f1), transcript variant 1 |
CNY 9,790.00 |
