Phf1 (NM_009343) Mouse Tagged ORF Clone
CAT#: MR223650
- TrueORF®
Phf1 (Myc-DDK-tagged) - Mouse PHD finger protein 1 (Phf1)
ORF Plasmid: tGFP
"NM_009343" in other vectors (4)
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CNY 4,160.00
CNY 4,940.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AW557215; D17Ertd455; D17Ertd455e; mPc; Pcl1; PHF; Phf2; Tctex3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR223650 representing NM_009343
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGCAGCTCCCCCGGCTGAGCCGTTTGGGTGCCCCCTCTCTCTGGGATCCAGCTTCCCCTGCTCCCA CCTCAGGCCCCAGACCTCGGCTTTGGGAAGGCCAAGATGTGCTGGCCAGATGGACTGATGGGCTGCTGTA CTTGGGTACCATCAAAAAGGTGGACAGTGCTCGAGAGGTGTGTCTGGTCCAGTTTGAGGACGATTCCCAG TTTCTGGTTCTATGGAAGGATATCAGCCCAGCTGCCCTCCCTGGGGAGGAGCTGCTCTGTTGTGTGTGTC GCTCTGAGACCGTGGTACCTGGGAACCGGCTGGTCAGCTGTGAGAAGTGTCGCCATGCTTATCACCAGGA CTGTCACGTTCCCAGGGCCCCGGCCCCTGGAGAAGGAGAGGGCGCATCCTGGGTATGCCGCCAGTGTGTC TTTGCAATCGCCACTAAGAGGGGAGGCGCACTGAAGAAAGGTCCCTATGCCCGGGCCATGCTGGGCATGA AGCTCTCCCTGCCATACGGATTAAAAGGGCTGGACTGGGATGCTGGACATTTGAGCAACAGACAGCAGAG CTACTGCTACTGTGGAGGCCCTGGAGAGTGGAACCTGAAAATGCTGCAGTGCCGGAGCTGCCTCCAGTGG TTCCATGAGGCCTGCACCCAGTGTCTGAGCAAGCCCCTCCTCTACGGAGACAGATTCTATGAATTTGAGT GCTGTGTGTGCCGGGGCGGCCCTGAGAAGGTCCGGAGGTTACAGCTTCGCTGGGTGGATGTGGCCCATCT TGTCCTCTACCACCTCAGCGTTTGCTGTAAGAAGAAATATTTTGATTTTGACCGAGAGATCCTCCCCTTC ACCTCTGAGAATTGGGACAGTCTGCTCCTGGGGGAGCTCTCAGATACTCCCAAGGGAGAGCGCTCTTCCC AGCTCCTTTCTGCTCTTAACAGCCACAAGGACCGTTTCATTTCAGGGAGGGAGATTAAAAAGCGAAAATG TCTTTTTGGTCTCCATGCTCGGACCCCTCCTCCTGTGGAGCTTCTCACTGGAGATGGAGCCCCCACCAGC TTCCCTTCAGGGCAGGGCCCTGGGGGAGGGGTCTCACGTCCCCTGGGGAAACGATGGAGGTCGGAGCCAG AACCCTTAAGGAGGAGGCAGAAGGGGAAAGTGGAGGAACTGGGGCCACCCACGGCAGCACACAGTCGGCA TGGGTCCCGGGAGCAGAGGGCCCTGCAGGCCTCGGTGTCTCCACCACCCCCCAGCCCTAACCAGAGCTAT GAGGGCAGCAGCGGCTACAACTTCCGGCCCACAGACGCCCGCTGTCTGCCCAGCAGCCCTATCCGGATGT TCGCCTCCTTCCACCCTTCTGCCAGCACTGCAGGGACCTCTGGGGACAGTGAACCCCCAGATAGGTCACC TCTGGGACTTCACATTGGCTTCCCCACAGACACCCCTAAAAGTTCACCCCACTCAGTGACGGCCTCATCT TCCTCGGTCCCAGCCCTGACCCCAGGCTTTTCCAGACATTCACCCCCTTCTCCCTTGTGCCGTAGTCTGT CTCCGGGGACTGGGGGAGGAGTCCGAGGTGGGGTTAGCTACCTGTCCCGAGGGGACCCTGTGAGGGTCCT TGCTCGAAGAGTGCGGCCTGACGGCTCTGTGCAGTACCTAGTTGAGTGGGGAGGGGGGGGCATCTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223650 representing NM_009343
Red=Cloning site Green=Tags(s) MAQLPRLSRLGAPSLWDPASPAPTSGPRPRLWEGQDVLARWTDGLLYLGTIKKVDSAREVCLVQFEDDSQ FLVLWKDISPAALPGEELLCCVCRSETVVPGNRLVSCEKCRHAYHQDCHVPRAPAPGEGEGASWVCRQCV FAIATKRGGALKKGPYARAMLGMKLSLPYGLKGLDWDAGHLSNRQQSYCYCGGPGEWNLKMLQCRSCLQW FHEACTQCLSKPLLYGDRFYEFECCVCRGGPEKVRRLQLRWVDVAHLVLYHLSVCCKKKYFDFDREILPF TSENWDSLLLGELSDTPKGERSSQLLSALNSHKDRFISGREIKKRKCLFGLHARTPPPVELLTGDGAPTS FPSGQGPGGGVSRPLGKRWRSEPEPLRRRQKGKVEELGPPTAAHSRHGSREQRALQASVSPPPPSPNQSY EGSSGYNFRPTDARCLPSSPIRMFASFHPSASTAGTSGDSEPPDRSPLGLHIGFPTDTPKSSPHSVTASS SSVPALTPGFSRHSPPSPLCRSLSPGTGGGVRGGVSYLSRGDPVRVLARRVRPDGSVQYLVEWGGGGIF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_009343 |
ORF Size | 1677 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_009343.3, NP_033369.2 |
RefSeq Size | 2429 bp |
RefSeq ORF | 1680 bp |
Locus ID | 21652 |
UniProt ID | Q9Z1B8 |
MW | 61.6 kDa |
Gene Summary | The protein encoded by this gene belongs to the polycomb-like protein family, which is a component of polycomb repressive complex-2. This complex represses gene expression by catalyzing the trimethylation of histone H3 lysine 27 and is required for the regulation of developmental genes including homeotic genes. The gene is expressed primarily in testis tissue. Small interfering RNA-mediated knockdown in cultured cell lines results in changes in homeotic gene expression coincident with alterations in promoter methylation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2014] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC218965 | Phf1 (untagged) - Mouse PHD finger protein 1 (Phf1), (10ug) |
CNY 7,130.00 |
|
MG223650 | Phf1 (tGFP-tagged) - Mouse PHD finger protein 1 (Phf1), (10ug) |
CNY 5,420.00 |
|
MR223650L3 | Lenti ORF clone of Phf1 (Myc-DDK-tagged) - Mouse PHD finger protein 1 (Phf1) |
CNY 6,840.00 |
|
MR223650L4 | Lenti ORF clone of Phf1 (mGFP-tagged) - Mouse PHD finger protein 1 (Phf1) |
CNY 6,840.00 |