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Atg4c (NM_175029) Mouse Tagged ORF Clone
CAT＃: MR223616
- TrueORF®
Atg4c (Myc-DDK-tagged) - Mouse autophagy-related 4C (yeast) (Atg4c), transcript variant 1
ORF Plasmid: tGFP
"NM_175029" in other vectors (3)
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CNY 3,656.00
CNY 4,090.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Apg4-C; Apg4c; Atg4cl; Autl1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223616 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGGCTTCAGGAACAGATGAAGTTGACAAGCTAAAAACTAAATTTATATCTGCTTGGAACAATATGA AATATAGTTGGGTGTTGAAAACAAAGACATATTTTAGTAGAAATTCACCTGTTTTATTGCTTGGAAAATG TTATCATTTTAAATATGAAGATGAAAGCAAGATGTTGCCTGCAAGATCAGGATGTGCCATTGAAGATCAT GTAATTGCAGGGAATGTGGAAGAGTTCCGTAAGGATTTCATTTCTAGATTATGGCTGACCTACAGGGAAG AATTTCCTCAAATCGAAGCTTCAGCCTTGACGACAGACTGTGGCTGGGGCTGCACGCTGCGGACCGGCCA GATGCTGCTGGCTCAGGGACTCATACTCCATTTTCTCGGTAGAGCTTGGACCTGGCCTGATGCGCTGCAC ATTGAGAACGCAGACTCTGACTCATGGACTTCCAACACTGTCAAAAAGTTTACTGCATCGTTTGAAGCTT CACTTTCGGGGGACAGAGAACTCAGAACTCCAGCAGTTTCTCTGAAGGAAACATCTGGGAAGTGTCCCGA TGACCACGCAGTGCGGAATGAGGCTTATCACAGGAAGATCATCTCTTGGTTTGGAGATTCCCCCGTGGCC GTCTTTGGCTTACATCGTCTGATAGAGTTTGGGAAGAAGTCTGGGAAAAAAGCTGGCGATTGGTATGGAC CAGCGGTGGTTGCTCACATTTTAAGAAAAGCAGTTGAAGAAGCAAGGCATCCGGATTTGCAAGGGCTAAC TATTTATGTTGCCCAGGACTGTACAGTTTACAATTCTGACGTGATTGATAAGCAGACTGACTCCGTGACA GCTGGAGATGCACGTGACAAGGCTGTCATTATTTTAGTTCCTGTTAGACTCGGTGGAGAAAGGACCAATA CCGACTATCTGGAGTTTGTGAAGGGTGTTTTAAGCCTTGAATACTGTGTAGGTATCATTGGCGGCAAACC TAAACAGTCATATTACTTTGCTGGATTTCAAGATGACAGTTTGATTTACATGGATCCTCATTACTGCCAG TCTTTTGTAGATGTCAGCATAAAGGATTTCCCTCTCGAGACATTCCACTGTCCATCTCCTAAAAAGATGT CATTTCGAAAAATGGATCCTAGTTGTACGATAGGATTTTACTGTCGAAATGTCCAGGACTTTGAACGAGC GTCTGAAGAAATCACTAAGATGTTGAAAATTTCCTCTAAGGAGAAATATCCACTATTTACTTTTGTAAAT GGCCATTCCAAAGACTTTGATTTTACATCTACCGCAGCCAGTGAAGAAGACCTTTTCTCAGAAGATGAAA GGAAGAATTTCAAAAGATTTAGCACAGAAGAGTTTGTCCTCCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223616 protein sequence
Red=Cloning site Green=Tags(s) MEASGTDEVDKLKTKFISAWNNMKYSWVLKTKTYFSRNSPVLLLGKCYHFKYEDESKMLPARSGCAIEDH VIAGNVEEFRKDFISRLWLTYREEFPQIEASALTTDCGWGCTLRTGQMLLAQGLILHFLGRAWTWPDALH IENADSDSWTSNTVKKFTASFEASLSGDRELRTPAVSLKETSGKCPDDHAVRNEAYHRKIISWFGDSPVA VFGLHRLIEFGKKSGKKAGDWYGPAVVAHILRKAVEEARHPDLQGLTIYVAQDCTVYNSDVIDKQTDSVT AGDARDKAVIILVPVRLGGERTNTDYLEFVKGVLSLEYCVGIIGGKPKQSYYFAGFQDDSLIYMDPHYCQ SFVDVSIKDFPLETFHCPSPKKMSFRKMDPSCTIGFYCRNVQDFERASEEITKMLKISSKEKYPLFTFVN GHSKDFDFTSTAASEEDLFSEDERKNFKRFSTEEFVLL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_175029 |
| ORF Size | 1377 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_175029.2 |
| RefSeq Size | 3236 bp |
| RefSeq ORF | 1377 bp |
| Locus ID | 242557 |
| UniProt ID | Q811C2 |
| MW | 52.1 kDa |
| Gene Summary | Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3 and GABARAPL2, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms (By similarity). Is not essential for autophagy development under normal conditions but is required for a proper autophagic response under stressful conditions such as prolonged starvation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG223616 | Atg4c (tGFP-tagged) - Mouse autophagy-related 4C (yeast) (Atg4c) transcript variant 1, (10ug) |
CNY 4,470.00 |
|
| MR223616L3 | Lenti ORF clone of Atg4c (Myc-DDK-tagged) - Mouse autophagy-related 4C (yeast) (Atg4c), transcript variant 1 |
CNY 5,990.00 |
|
| MR223616L4 | Lenti ORF clone of Atg4c (mGFP-tagged) - Mouse autophagy-related 4C (yeast) (Atg4c), transcript variant 1 |
CNY 5,990.00 |
