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Plag1 (NM_019969) Mouse Tagged ORF Clone
CAT＃: MR223145
- TrueORF®
Plag1 (Myc-DDK-tagged) - Mouse pleiomorphic adenoma gene 1 (Plag1)
ORF Plasmid: tGFP
"NM_019969" in other vectors (4)
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CNY 3,656.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223145 representing NM_019969
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCACTGTCATTCCTGGTGATTTGTCAGAAGTAAGAGATACCCAGAAAGCCCCTTCAGGGAAACGTA AGCGTGGTGAAAGCAAACCAAGAAAAAACTTTCCTTGCCAACTGTGTGACAAGGCCTTTAACAGTGTTGA GAAATTAAAGGTTCACTCCTTCTCTCACACGGGAGAGAGGCCCTACAAGTGCACACACCAAGACTGCACC AAGGCCTTTGTTTCTAAGTACAAATTACAAAGGCACATGGCTACTCACTCTCCTGAGAAAACCCACAAGT GTAATTACTGTGAGAAAATGTTTCACCGGAAAGACCATCTGAAGAATCACCTCCATACACATGACCCCAA CAAAGAGACCTTTAAATGCGAAGAGTGTGGCAAGAGCTACAATACCAAGCTTGGTTTTAAGCGACACTTG GCCTTGCATGCTGCCACCAGTGGTGACCTCACCTGCAAGGTGTGTTTGCAGAATTTTGAGAGCACAGGTG TGCTCCTAGAGCACCTGAAATCTCACGCGGGCAAGTCATCTGGGGGTGTGAAGGAGAAAAAGCACCAATG TGAGCACTGCGAACGCAGGTTCTACACCCGGAAGGATGTCCGGAGACACATGGTGGTACACACAGGAAGA AAGGACTTCCTCTGTCAGTACTGCGCACAGAGATTTGGACGAAAGGATCACCTCACTCGACACATGAAGA AGAGTCACAATCAGGAGCTTCTGAAGGTCAAAACGGAACCAGTAGATTTCCTGGACCCATTTACCTGCAA CATGTCTGTGCCTATAAAAGATGAACTCCTGCCGGTCATGTCCTTACCTTCCAGTGAACTCTTGTCAAAG CCATTCACAAACACTTTGCAGTTAAACCTCTATAACACTCCATTTCAGTCCATGCAGAGCTCTGGGTCTG CTCACCAAATGATAACAACTTTACCTTTGGGAATGACATGCCCCATAGATATGGATGCTGTTCACCCCTC TCATCATCTTGCTTTCAAATGCCCATTCAGTTCTACCTCATACGCAATCTCTATTCCTGAAAAAGAACAG CCATTAAAGGGGGAAATTGAGAGTTACCTGATGGAGCTACAAGGTGGTGCACCATCTTCATCCCAGGATT CTCCAGCATCGTCATCTAAGCTAGGGTTAGAGCCTCAGAGTGGGTCGCCAGATGATGGTGCTGGGGACCT CTCCCTGTCAAAGAGCTCTATCTCTATCAGTGACCCCCTCAGCACACCAGCATTGGATTTCTCTCAGTTG TTCAATTTCATACCATTGAATGGTCCCCCTTATAACCCACTTTCAGTGGGAAGCCTTGGGATGAGCTATT CCCAGGAGGAAGCACACTCTTCTGTCTCTCAGCTGCCCACACAAACACAGGATCTTCAGGACCCTGCAAA CACTGTGGGTCTAAGTTCTCTGCACTCACTGTCGGCAGCGTTCACCAGCAGCCTGAGCTCAAGCACTACC TTGCCCCGTTTCCACCAGGCGTTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223145 representing NM_019969
Red=Cloning site Green=Tags(s) MATVIPGDLSEVRDTQKAPSGKRKRGESKPRKNFPCQLCDKAFNSVEKLKVHSFSHTGERPYKCTHQDCT KAFVSKYKLQRHMATHSPEKTHKCNYCEKMFHRKDHLKNHLHTHDPNKETFKCEECGKSYNTKLGFKRHL ALHAATSGDLTCKVCLQNFESTGVLLEHLKSHAGKSSGGVKEKKHQCEHCERRFYTRKDVRRHMVVHTGR KDFLCQYCAQRFGRKDHLTRHMKKSHNQELLKVKTEPVDFLDPFTCNMSVPIKDELLPVMSLPSSELLSK PFTNTLQLNLYNTPFQSMQSSGSAHQMITTLPLGMTCPIDMDAVHPSHHLAFKCPFSSTSYAISIPEKEQ PLKGEIESYLMELQGGAPSSSQDSPASSSKLGLEPQSGSPDDGAGDLSLSKSSISISDPLSTPALDFSQL FNFIPLNGPPYNPLSVGSLGMSYSQEEAHSSVSQLPTQTQDLQDPANTVGLSSLHSLSAAFTSSLSSSTT LPRFHQAFQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019969 |
| ORF Size | 1497 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019969.3, NP_064353.2 |
| RefSeq Size | 4635 bp |
| RefSeq ORF | 1500 bp |
| Locus ID | 56711 |
| UniProt ID | Q9QYE0 |
| MW | 56 kDa |
| Gene Summary | Transcription factor whose activation results in up-regulation of target genes, such as IGFII, leading to uncontrolled cell proliferation: when overexpressed in cultured cells, higher proliferation rate and transformation are observed. Other target genes such as CRLF1, CRABP2, CRIP2, PIGF are strongly induced in cells with PLAG1 induction. Proto-oncogene whose ectopic expression can trigger the development of pleomorphic adenomas of the salivary gland and lipoblastomas. Cooperates with CBFB-MYH11.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216999 | Plag1 (untagged) - Mouse pleiomorphic adenoma gene 1 (Plag1), (10ug) |
CNY 6,460.00 |
|
| MG223145 | Plag1 (tGFP-tagged) - Mouse pleiomorphic adenoma gene 1 (Plag1), (10ug) |
CNY 3,710.00 |
|
| MR223145L3 | Lenti ORF clone of Plag1 (Myc-DDK-tagged) - Mouse pleiomorphic adenoma gene 1 (Plag1) |
CNY 5,230.00 |
|
| MR223145L4 | Lenti ORF clone of Plag1 (mGFP-tagged) - Mouse pleiomorphic adenoma gene 1 (Plag1) |
CNY 5,230.00 |
