Cask (NM_009806) Mouse Tagged ORF Clone

CAT#: MR223129

  • TrueORF®

Cask (Myc-DDK-tagged) - Mouse calcium/calmodulin-dependent serine protein kinase (MAGUK family) (Cask)

ORF Plasmid: DDK tGFP



  "NM_009806" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 6,576.00

CNY 7,890.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms DXPri1; DXRib1; LIN-2; mLin-2; Pals3
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR223129 representing NM_009806
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGCCGACGACGACGTGCTGTTCGAGGATGTGTACGAGCTATGCGAGGTGATCGGCAAGGGTCCCTTCA
GTGTTGTACGGCGATGTATCAACAGAGAAACTGGGCAACAATTTGCTGTAAAAATTGTTGATGTAGCCAA
GTTCACATCAAGTCCAGGGTTAAGTACAGAAGATCTAAAGCGGGAAGCCAGTATCTGTCATATGCTGAAG
CATCCACACATTGTAGAGCTGTTGGAGACATATAGCTCAGATGGGATGCTTTACATGGTTTTTGAATTTA
TGGATGGAGCAGATCTGTGTTTTGAAATCGTAAAGCGAGCTGATGCTGGTTTTGTATACAGTGAAGCTGT
AGCCAGCCACTACATGAGACAGATACTGGAAGCTCTGCGCTACTGTCATGATAATAACATAATTCACAGG
GACGTGAAGCCCCACTGTGTTCTCCTTGCCTCAAAAGAAAACTCGGCACCTGTTAAACTTGGGGGCTTTG
GGGTGGCCATTCAGTTAGGAGAATCTGGACTTGTTGCTGGAGGCCGCGTTGGAACACCTCACTTTATGGC
CCCAGAAGTGGTCAAGAGAGAGCCTTACGGAAAGCCTGTGGATGTCTGGGGCTGTGGTGTGATCCTTTTC
ATCCTGCTCAGTGGCTGTTTGCCTTTCTACGGAACCAAGGAAAGATTGTTTGAAGGCATTATTAAAGGAA
AATATAAGATGAATCCAAGGCAGTGGAGCCATATCTCTGAAAGTGCCAAAGACCTAGTACGCCGCATGCT
GATGCTGGATCCTGCTGAAAGGATCACTGTTTATGAAGCACTGAATCACCCATGGCTTAAGGAGCGGGAT
CGTTATGCCTACAAAATCCATCTTCCAGAAACAGTAGAACAACTGAGGAAATTCAATGCAAGGAGAAAAC
TAAAGGGTGCAGTACTAGCTGCTGTGTCAAGTCACAAATTCAATTCCTTCTATGGGGACCCTCCTGAAGA
GTTGCCAGATTTCTCCGAAGACCCTACCTCCTCAGGAGCCGTCTCTCAGGTGCTGGACAGCCTGGAAGAG
ATTCACGCACTTACAGACTGCAGTGAAAAGGACCTAGATTTTCTACACAGTGTTTTCCAGGATCAACATC
TTCACACACTGCTGGATCTGTATGACAAAATTAACACAAAGTCTTCGCCACAAATCAGGAATCCTCCAAG
TGATGCAGTACAGAGAGCCAAAGAGGTATTGGAAGAAATTTCATGTTACCCTGAGAATAATGATGCGAAG
GAACTAAAGCGTATTTTAACACAACCTCATTTCATGGCCTTACTTCAGACTCATGATGTAGTGGCACATG
AAGTTTACAGTGATGAAGCATTAAGGGTCACACCTCCCCCGACTTCCCCCTATTTAAACGGTGATTCTCC
AGAAAGTGCAAACGGAGACATGGACATGGAGAATGTGACCAGAGTTCGGCTGGTACAGTTCCAAAAGAAC
ACGGATGAGCCAATGGGAATCACTTTGAAAATGAATGAGCTAAATCATTGTATTGTCGCAAGAATCATGC
ATGGGGGTATGATTCACAGGCAAGGTACACTTCATGTTGGTGATGAAATCCGAGAAATCAATGGCATCAG
TGTCGCTAACCAAACAGTGGAACAGCTACAGAAAATGCTTAGGGAAATGCGAGGGAGTATTACCTTCAAG
ATTGTGCCAAGCTACCGCACTCAGTCTTCGTCCTGTGAGGACTTGCCATCAACCACCCAACCAAAAGGAC
GACAGATCTATGTAAGAGCACAATTTGAATATGATCCAGCCAAGGATGACCTCATCCCCTGCAAAGAAGC
TGGCATCCGGTTCCGAGTTGGTGACATCATCCAGATTATTAGTAAGGATGACCACAACTGGTGGCAGGGT
AAACTGGAAAACTCCAAAAATGGAACTGCAGGTCTCATTCCTTCTCCTGAACTTCAGGAATGGCGAGTAG
CTTGCATTGCCATGGAGAAGACCAAACAAGAGCAGCAGGCCAGCTGTACTTGGTTTGGCAAGAAAAAGAA
GCAGTACAAAGATAAATATTTGGCAAAGCACAACGCAGTGTTTGATCAATTAGATCTTGTCACATATGAA
GAAGTAGTCAAACTGCCAGCATTCAAAAGGAAAACATTAGTCTTATTAGGTGCACATGGTGTTGGAAGAA
GACACATAAAAAATACCCTCATCACAAAGCACCCGG[AT]CCGGTTTGCGTACCCTATTCCACATACAAC
CAGACCTCCAAAGAAAGATGAAGAAAATGGCAAGAATTATTACTTTGTATCTCATGACCAAATGATGCAA
GACATCTCAAATAACGAATACTTGGAGTATGGCAGCCATGAGGATGCAATGTACGGGACAAAACTGGAGA
CCATTCGGAAAATCCATGAGCAGGGGCTGATTGCGATTCTGGACGTGGAGCCTCAGGCACTGAAGGTCCT
GAGGACTGCAGAGTTTGCTCCTTTTGTTGTCTTCATTGCGGCGCCAACTATCACTCCAGGTTTAAATGAG
GATGAATCTCTTCAGCGCCTGCAGAAGGAGTCCGATGTCTTGCAGAGAACATATGCACACTACTTCGATC
TCACAATTATCAACAACGAAATTGATGAGACAATCAGACATCTGGAAGAAGCTGTCGAGCTTGTGTGCAC
AGCCCCACAGTGGGTCCCAGTCTCCTGGGTCTAT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR223129 representing NM_009806
Red=Cloning site Green=Tags(s)

MADDDVLFEDVYELCEVIGKGPFSVVRRCINRETGQQFAVKIVDVAKFTSSPGLSTEDLKREASICHMLK
HPHIVELLETYSSDGMLYMVFEFMDGADLCFEIVKRADAGFVYSEAVASHYMRQILEALRYCHDNNIIHR
DVKPHCVLLASKENSAPVKLGGFGVAIQLGESGLVAGGRVGTPHFMAPEVVKREPYGKPVDVWGCGVILF
ILLSGCLPFYGTKERLFEGIIKGKYKMNPRQWSHISESAKDLVRRMLMLDPAERITVYEALNHPWLKERD
RYAYKIHLPETVEQLRKFNARRKLKGAVLAAVSSHKFNSFYGDPPEELPDFSEDPTSSGAVSQVLDSLEE
IHALTDCSEKDLDFLHSVFQDQHLHTLLDLYDKINTKSSPQIRNPPSDAVQRAKEVLEEISCYPENNDAK
ELKRILTQPHFMALLQTHDVVAHEVYSDEALRVTPPPTSPYLNGDSPESANGDMDMENVTRVRLVQFQKN
TDEPMGITLKMNELNHCIVARIMHGGMIHRQGTLHVGDEIREINGISVANQTVEQLQKMLREMRGSITFK
IVPSYRTQSSSCEDLPSTTQPKGRQIYVRAQFEYDPAKDDLIPCKEAGIRFRVGDIIQIISKDDHNWWQG
KLENSKNGTAGLIPSPELQEWRVACIAMEKTKQEQQASCTWFGKKKKQYKDKYLAKHNAVFDQLDLVTYE
EVVKLPAFKRKTLVLLGAHGVGRRHIKNTLITKHPXXRFAYPIPHTTRPPKKDEENGKNYYFVSHDQMMQ
DISNNEYLEYGSHEDAMYGTKLETIRKIHEQGLIAILDVEPQALKVLRTAEFAPFVVFIAAPTITPGLNE
DESLQRLQKESDVLQRTYAHYFDLTIINNEIDETIRHLEEAVELVCTAPQWVPVSWVY

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_009806
ORF Size 2694 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Note Plasmids are not sterile.  For experiments where strict sterility is required, filtration with 0.22um filter is required.
Reference Data
RefSeq NM_009806.2, NM_009806.3, NP_033936.2
RefSeq Size 8260 bp
RefSeq ORF 2694 bp
Locus ID 12361
UniProt ID O70589
MW 102.5 kDa
Gene Summary Multidomain scaffolding protein with a role in synaptic transmembrane protein anchoring and ion channel trafficking. Contributes to neural development and regulation of gene expression via interaction with the transcription factor TBR1. Binds to cell-surface proteins, including amyloid precursor protein, neurexins, and syndecans. May mediate a link between the extracellular matrix and the actin cytoskeleton via its interaction with syndecan and with the actin/spectrin-binding protein 4.1.[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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