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Astn2 (NM_019514) Mouse Tagged ORF Clone
CAT＃: MR223104
- TrueORF®
Astn2 (Myc-DDK-tagged) - Mouse astrotactin 2 (Astn2), transcript variant 1
ORF Plasmid: tGFP
"NM_019514" in other vectors (4)
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CNY 9,048.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1d8; Astnl; bM452J22.1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223104 representing NM_019514
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCGCCGCCGGCGCCCGGCGCAGCCCGGGCCGCGGCTTGGGGCTTCGGGGGCGGCCGAGGCTCGGCT TCCACCCGGGGCCGCCGCCGCCACCGCCGCCGCCGCTGCTGTTGCTGTTTCTGCTCCTGCTGCCGCCGCC GCCGTTGCTGGCCGGCGCCACGGCCGCCGCCGCCTCGCGGGAGCCCGACAGCCCATGCCGGCTCAAGACC GTCACCGTATCCACGCTGCCCGCTCTGCGGGAAAGCGACATCGGCTGGAGCGGTGCCCGCACCGGGGCCG CGGCCGGGGCTGGGGCCGGGACCGGAGCCGGGGCCGGAGCTGCTGCTGCTGCCGCCTCCGCCGCGTCCCC GGGCTCCGCGGGTTCAGCCGGCACAGCCGCGGAGTCTCGTCTCCTTCTCTTTGTGCGTAATGAGCTCCCC GGGCGCATCGCGGTACAAGATGACCTGGACAACACTGAGCTGCCCTTCTTCACCCTGGAGATGTCTGGCA CAGCAGCGGACATTTCTTTGGTTCACTGGAGGCAGCAGTGGCTGGAGAATGGCACATTGTACTTCCACGT TTCCATGAGCAGCTCTGGGCAACTGGCTCAGGCCACTGCTCCCACACTCCAGGAGCCCTCGGAGATCGTT GAGGAACAGATGCATATCCTCCACATTTCTGTGATGGGTGGACTCATTGCACTTCTCCTCCTGCTGTTGG TGTTCACAGTGGCACTGTATGCCCAGCGACGCTGGCAGAAGCGCCGGCGCATCCCACAGAAGAGCGCAAG TACAGAAGCTACTCATGAGATTCACTACATCCCATCAGTGCTGCTGGGTCCTCAGGCCAGGGAAAGCTTC CGATCCTCCAGACTTCAGACACACAACTCAGTCATTGGTGTGCCTATCCGGGAAACCCCCATCCTGGATG ACTATGACTATGAAGAGGAGGAGGAACCACCCAGGCGAGCCAACCACGTCTCCCGTGAGGATGAGTTTGG TAGCCAGATGACCCATGCCCTGGACAGCTTGGGAAGGCCAGGAGAAGAGAAAGTGGAATTTGAGAAGAAA GGGGGAATCAGCTTTGGGAGAACCAAGGGGACATCAGGCTCAGAAGCAGATGACGAAACACAGCTGACCT TCTACACAGAGCAGTACCGCAGTCGCCGCCGCAGCAAAGGTTTACTGAAAAGCCCTGTGAATAAGACAGC CTTAACACTGATTGCTGTGAGCTCCTGCATCTTGGCCATGGTGTGTGGCAACCAGATGTCCTGTCCACTT ACTGTGAAGGTGACTTTGCATGTGCCTGAACACTTCATTGCAGATGGGAGCAGCTTTGTGGTGAGTGAAG GAAGCTACCTGGATATCTCCGATTGGCTAAACCCTGCCAAGCTGTCTCTGTATTACCAGATCAATGCCAC CTCGCCATGGGTGAGAGACCTCTGTGGACAGAGGACGACAGATGCCTGTGAACAGCTCTGTGACCCAGAT ACTGGGGAGTGCAGTTGTCATGAAGGTTACGCCCCTGACCCAGTCCACAGACATCTATGTGTTCGCAGTG ACTGGGGTCAAAGTGAAGGACCTTGGCCTTACACAACACTGGAGAGGGGCTATGATCTGGTGACAGGAGA GCAAGCCCCTGAAAAGATTCTCAGGTCTACTTTCAGCTTGGGCCAAGGCCTCTGGCTTCCGGTCAGCAAA AGCTTTGTGGTGCCACCGGTGGAGCTGTCCATCAACCCCCTGGCCAGCTGCAAGACGGATGTGCTCGTCA CAGAAGACCCTGCGGATGTCAGGGAAGAAGCAATGTTGTCCACGTACTTTGAAACCATCAACGACCTGCT GTCCTCCTTTGGTCCAGTCCGTGACTGCTCAAGGAACAACGGGGGCTGTACCCGTAACTTCAAGTGTGTG TCTGACCGCCAGGTGGACTCCTCGGGATGTGTGTGTCCTGAGGAACTGAAGCCCATGAAGGATGGCTCTG GCTGCTATGATCACTCCAAGGGCATCGACTGCTCTGATGGCTTTAATGGAGGGTGTGAGCAGCTTTGCCT ACAACAAACACTGCCTTTGCCCTATGATACCACCTCCAGCACCATCTTCATGTTCTGCGGCTGTGTGGAA GAGTACAAGCTGGCTCCTGATGGGAAATCCTGTCTAATGCTCTCAGATGTCTGTGAGGGCCCCAAGTGCC TCAAACCTGACTCCAAATTCAATGACACCCTCTTTGGAGAGATGCTACATGGTTACAACAACCGGACACA ACATGTAAACCAAGGCCAAGTCTTCCAAATGACATTCAGGGAGAATAACTTCATCAAGGACTTTCCACAG CTGGCAGATGGGCTGTTGGTGATCCCGCTGCCAGTGGAAGAGCAATGCCGGGGGGTTCTCTCTGAGCCCC TTCCAGACCTTCAGCTTCTCACAGGAGACATCAGGTATGATGAGGCCATGGGGTACCCCATGGTGCAACA GTGGCGTGTCCGGAGCAATCTCTATCGTGTGAAGCTGAGTACTATCACCCTCTCAGCAGGCTTCACCAAT GTCCTAAAGATCCTGACCAAAGAGAGCAGTCGGGATGAGCTGCTGTCCTTCATCCAGCACTATGGCTCCC ACTACATTGCCGAGGCTTTATACGGTTCTGAGCTCACCTGCATCATCCATTTTCCCAGCAAGAAGGTCCA GCAGCAGCTATGGCTCCAATATCAGAAAGAGACCACGGAGCTGGGCAGCAAGAAGGAGCTCAAGTCCATG CCCTTCATCACCTACCTCTCCGGTCTCCTGACAGCCCAGATGCTATCAGATGACCAGCTCATCTCAGGTG TGGAGATTCGATGTGAGGAGAAGGGCCGCTGTCCATCTACCTGTCACCTTTGCCGCCGGCCAGGCAAAGA GCAGCTGAGCCCCACACCAGTGCTGCTGGAGATAAACCGTGTGGTTCCACTTTATACCCTCATCCAAGAC AATGGCACAAAGGAGGCCTTCAAGAATGCACTGATGAGTTCTTACTGGTGTTCTGGTAAAGGAGATGTGA TTGATGACTGGTGCAGGTGTGACCTCAGTGCCTTTGATGCCAGTGGGCTTCCAAATTGTAGCCCCCTTCC ACAGCCGGTGTTACGACTTTCTCCAACAGTGGAGCCTTCCAGCACTGTGGTTTCCCTGGAGTGGGTAGAT GTTCAGCCAGCCATTGGAACCAAAGTCTCTGACTATATTCTGCAGCACAAGAAAGTGGATGAATACACAG ACACGGACCTATACACAGGAGAATTCCTGAGTTTTGCCGATGACTTACTTTCTGGCCTGGGTACATCTTG TGTAGCAGCTGGTCGAAGCCATGGAGAGGTTCCTGAAGTCAGTATCTACTCGGTGATCTTCAAGTGCCTG GAGCCAGATGGTCTTTACAAGTTCACTCTGTATGCTGTGGATACTCGAGGGAGGCACTCAGAGCTCAGCA CAGTGACTCTAAGGACAGCTTGTCCACTGGTAGATGACAACAAAGCAGAAGAGATAGCGGACAAGATTTA CAATCTATACAATGGTTACACAAGTGGCAAGGAGCAGCAGACTGCTTACAACACACTGATGGAAGTCTCC GCCTCCATGCTCTTCCGTGTCCAGCACCACTACAACTCTCACTATGAAAAGTTTGGTGACTTCGTCTGGA GGAGTGAGGATGAGCTAGGACCAAGGAAGGCTCATCTGATTCTGCGGCGGCTGGAGCGGGTCAGCAGCCA CTGTTCTAGTCTCTTGAGAAGCGCCTACATCCAGAGTCGTGTGGACACCATACCCTATCTCTTCTGCCGC AGTGAGGAGGTCCGGCCTGCAGGAATGGTGTGGTACAGCATCCTCAAAGACACCAAAATCACATGTGAGG AGAAGATGGTGTCTATGGCCCGAAACACGTACGGGGAAACCAAAGGCCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223104 representing NM_019514
Red=Cloning site Green=Tags(s) MAAAGARRSPGRGLGLRGRPRLGFHPGPPPPPPPPLLLLFLLLLPPPPLLAGATAAAASREPDSPCRLKT VTVSTLPALRESDIGWSGARTGAAAGAGAGTGAGAGAAAAAASAASPGSAGSAGTAAESRLLLFVRNELP GRIAVQDDLDNTELPFFTLEMSGTAADISLVHWRQQWLENGTLYFHVSMSSSGQLAQATAPTLQEPSEIV EEQMHILHISVMGGLIALLLLLLVFTVALYAQRRWQKRRRIPQKSASTEATHEIHYIPSVLLGPQARESF RSSRLQTHNSVIGVPIRETPILDDYDYEEEEEPPRRANHVSREDEFGSQMTHALDSLGRPGEEKVEFEKK GGISFGRTKGTSGSEADDETQLTFYTEQYRSRRRSKGLLKSPVNKTALTLIAVSSCILAMVCGNQMSCPL TVKVTLHVPEHFIADGSSFVVSEGSYLDISDWLNPAKLSLYYQINATSPWVRDLCGQRTTDACEQLCDPD TGECSCHEGYAPDPVHRHLCVRSDWGQSEGPWPYTTLERGYDLVTGEQAPEKILRSTFSLGQGLWLPVSK SFVVPPVELSINPLASCKTDVLVTEDPADVREEAMLSTYFETINDLLSSFGPVRDCSRNNGGCTRNFKCV SDRQVDSSGCVCPEELKPMKDGSGCYDHSKGIDCSDGFNGGCEQLCLQQTLPLPYDTTSSTIFMFCGCVE EYKLAPDGKSCLMLSDVCEGPKCLKPDSKFNDTLFGEMLHGYNNRTQHVNQGQVFQMTFRENNFIKDFPQ LADGLLVIPLPVEEQCRGVLSEPLPDLQLLTGDIRYDEAMGYPMVQQWRVRSNLYRVKLSTITLSAGFTN VLKILTKESSRDELLSFIQHYGSHYIAEALYGSELTCIIHFPSKKVQQQLWLQYQKETTELGSKKELKSM PFITYLSGLLTAQMLSDDQLISGVEIRCEEKGRCPSTCHLCRRPGKEQLSPTPVLLEINRVVPLYTLIQD NGTKEAFKNALMSSYWCSGKGDVIDDWCRCDLSAFDASGLPNCSPLPQPVLRLSPTVEPSSTVVSLEWVD VQPAIGTKVSDYILQHKKVDEYTDTDLYTGEFLSFADDLLSGLGTSCVAAGRSHGEVPEVSIYSVIFKCL EPDGLYKFTLYAVDTRGRHSELSTVTLRTACPLVDDNKAEEIADKIYNLYNGYTSGKEQQTAYNTLMEVS ASMLFRVQHHYNSHYEKFGDFVWRSEDELGPRKAHLILRRLERVSSHCSSLLRSAYIQSRVDTIPYLFCR SEEVRPAGMVWYSILKDTKITCEEKMVSMARNTYGETKGR TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019514 |
| ORF Size | 3900 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019514.3, NP_062387.2 |
| RefSeq Size | 4675 bp |
| RefSeq ORF | 3903 bp |
| Locus ID | 56079 |
| UniProt ID | Q80Z10 |
| MW | 144.1 kDa |
| Gene Summary | Mediates recycling of the neuronal cell adhesion molecule ASTN1 to the anterior pole of the cell membrane in migrating neurons. Promotes ASTN1 internalization and intracellular transport of endocytosed ASTN1 (PubMed:20573900). Selectively binds inositol-4,5-bisphosphate, inositol-3,4,5-trisphosphate and inositol-1,3,4,5-tetrakisphosphate, suggesting it is recruited to membranes that contain lipids with a phosphoinositide headgroup (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224187 | Astn2 (untagged) - Mouse astrotactin 2 (Astn2), transcript variant 1, (10ug) |
CNY 16,720.00 |
|
| MG223104 | Astn2 (tGFP-tagged) - Mouse astrotactin 2 (Astn2) transcript variant 1, (10ug) |
CNY 12,540.00 |
|
| MR223104L3 | Lenti ORF clone of Astn2 (Myc-DDK-tagged) - Mouse astrotactin 2 (Astn2), transcript variant 1 |
CNY 13,300.00 |
|
| MR223104L4 | Lenti ORF clone of Astn2 (mGFP-tagged) - Mouse astrotactin 2 (Astn2), transcript variant 1 |
CNY 13,300.00 |
