Arhgap1 (NM_001145902) Mouse Tagged ORF Clone
CAT#: MR222984
- TrueORF®
Arhgap1 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 1
ORF Plasmid: tGFP
"NM_001145902" in other vectors (4)
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CNY 3,656.00
CNY 4,280.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | B230365D05Rik; C76222; Cdc42GAP; p50rhoGAP |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222984 representing NM_001145902
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAAGTGGTTGGGGAGCTGGGGGCCCCCGAGGGGGCGGTTCCCATTCCCATCTCGGAGCCGGATG TTTGCCGAGCTTTGACAGGCGTGGCAGAGGAAGCATTGCCGGAACGAGCCATGGACCCTCTCTCAGAGCT GCAAGATGACCTGACGCTGGATGACACCAGCCAAGCTCTGAACCAGTTGAAGCTGGCCTCCATTGATGAG AAGAACTGGCCTTCTGATGAAATGCCAGACTTCCCCAAGTCAGATGACTCCAAAAGCAGCTCTCCAGAAC CTGTGACACACCTGAAGTGGGATGACCCTTACTATGACATTGCCCGGCACCAGATTGTGGAAGTAGCCGG TGATGATAAGTATGGGCGGAAGATCATTGTATTCAGTGCTTGCCGGATGCCCCCCAGCCACCAGCTGGAC CACAGCAAGCTCTTGGGGTATTTGAAACACACATTGGACCAGTATGTGGAGAGTGACTACACACTCCTGT ACCTGCACCATGGCCTGACCAGTGACAACAAGCCCTCCCTCAGCTGGCTACGGGATGCCTACCGAGAGTT TGACCGCAAGTACAAGAAGAACATCAAGGCTCTGTACATCGTGCACCCAACCATGTTCATCAAAACTCTG CTTATCCTCTTTAAGCCTCTCATCAGTTTCAAGTTTGGACGGAAGATCTTCTACGTGAATTACCTGAGTG AGCTGAGCGAGCACGTGAAGCTGGAGCAGTTGGGGATCCCTCGCCAAGTGCTCAAGTATGATGACTTCCT CAAATCCACACAGAAAAGCCCTGCAACAGCTCCCAAGCCCATGCCACCACGGCCCCCCCTGCCCAATCAG CAGTTTGGGGTCTCCTTACAGCACCTCCAGGAGAAAAGCCCAGGCCAGGACCCCATTCCCATTGTCCTCA GGGAGACCGTCGCCTACTTGCAGGCTCACGCTCTCACCACTGAGGGCATCTTCCGGAGGTCTGCCAACAC CCAGGTTGTCCGGGAAGTGCAGCAGAAATACAATATGGGGCTGCCGGTGGACTTTGACCAGTACAACGAG CTGCACTTGCCCGCTGTCATCCTCAAGACATTCCTTCGGGAGCTTCCTGAACCCTTGCTAACCTTTGACC TGTACCCCCATGTTGTGGGCTTCCTCAATATTGATGAAAGCCAGAGAGTGGAAGTGACACAGCAGGTTCT CCAAACTCTGCCAGAGGAGAACTACCAGGTGCTTCATTTCCTGACTGCCTTCCTGGTGCAGATATCTGCC CACTGTGACCAGAACAAGATGACCAACACTAACTTGGCTGTAGTCTTCGGCCCTAACCTGCTCTGGGCTA AAGATGCTGCCATCACCCTCAAGGCTATCAATCCCATCAACACCTTCACTAAGTTCCTCCTGGATCATCA AGGGGAATTGTTCCCCAGCACAGATGCCCAGGGGGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222984 representing NM_001145902
Red=Cloning site Green=Tags(s) MAEVVGELGAPEGAVPIPISEPDVCRALTGVAEEALPERAMDPLSELQDDLTLDDTSQALNQLKLASIDE KNWPSDEMPDFPKSDDSKSSSPEPVTHLKWDDPYYDIARHQIVEVAGDDKYGRKIIVFSACRMPPSHQLD HSKLLGYLKHTLDQYVESDYTLLYLHHGLTSDNKPSLSWLRDAYREFDRKYKKNIKALYIVHPTMFIKTL LILFKPLISFKFGRKIFYVNYLSELSEHVKLEQLGIPRQVLKYDDFLKSTQKSPATAPKPMPPRPPLPNQ QFGVSLQHLQEKSPGQDPIPIVLRETVAYLQAHALTTEGIFRRSANTQVVREVQQKYNMGLPVDFDQYNE LHLPAVILKTFLRELPEPLLTFDLYPHVVGFLNIDESQRVEVTQQVLQTLPEENYQVLHFLTAFLVQISA HCDQNKMTNTNLAVVFGPNLLWAKDAAITLKAINPINTFTKFLLDHQGELFPSTDAQGV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001145902 |
ORF Size | 1437 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001145902.1, NP_001139374.1 |
RefSeq Size | 2904 bp |
RefSeq ORF | 1440 bp |
Locus ID | 228359 |
UniProt ID | Q5FWK3 |
MW | 54.9 kDa |
Gene Summary | GTPase activator for the Rho, Rac and Cdc42 proteins, converting them to the putatively inactive GDP-bound state. Cdc42 seems to be the preferred substrate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC216716 | Arhgap1 (untagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 1, (10ug) |
CNY 6,270.00 |
|
MG222984 | Arhgap1 (tGFP-tagged) - Mouse Rho GTPase activating protein 1 (Arhgap1) transcript variant 1, (10ug) |
CNY 4,750.00 |
|
MR222984L3 | Lenti ORF clone of Arhgap1 (Myc-DDK-tagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 1 |
CNY 6,180.00 |
|
MR222984L4 | Lenti ORF clone of Arhgap1 (mGFP-tagged) - Mouse Rho GTPase activating protein 1 (Arhgap1), transcript variant 1 |
CNY 6,180.00 |
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