Scn5a (NM_021544) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mH1; Nav1.5; Nav1.5c; SkM1; SkM2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222902 representing NM_021544 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCAAACTTCCTGTTACCTCGGGGCACCAGCAGCTTCCGTAGGTTCACCCGGGAGTCACTGGCGGCCA TCGAGAAGCGCATGGCTGAAAAACAAGCCCGTGGTTCGGCCACCTCACAGGAGAGCCGTGAGGGCCTGCC AGAGGAGGAGGCTCCCCGGCCCCAGCTGGACCTACAGGCCTCCAAAAAGCTGCCAGATCTCTATGGCAAC CCACCCCGAGAGCTCATTGGGGAGCCCCTGGAAGACCTGGACCCTTTCTATAGTACCCAGAAGACCTTCA TCGTGCTGAATAAGGGCAAAACCATCTTCCGGTTCAGTGCCACCAATGCCTTGTACGTCCTCAGCCCCTT CCACCCGGTGCGCAGAGCGGCTGTGAAGATTTTGGTACATTCGCTCTTCAGCATGCTCATCATGTGCACC ATCCTAACCAACTGTGTGTTCATGGCACAACACGACCCTCCGCCTTGGACCAAATATGTTGAGTACACCT TCACCGCCATCTACACCTTTGAGTCTCTGGTCAAGATTCTAGCTCGAGGCTTCTGCCTGCATGCGTTCAC CTTCCTCCGGGACCCGTGGAACTGGCTAGACTTCAGTGTGATCGTCATGGCGTATGTATCAGAGAATATA AAGCTAGGCAATTTGTCGGCTCTTCGAACTTTCAGAGTCCTGAGAGCTCTGAAAACGATTTCAGTTATTC CAGGCCTGAAGACAATCGTGGGAGCCCTAATCCAGTCTGTGAAGAAGCTAGCCGATGTGATGGTCCTTAC TGTCTTCTGCCTCAGTGTCTTTGCCCTCATTGGCCTGCAGCTCTTCATGGGCAACCTGAGGCACAAGTGC GTGCGTAACTTTACTGAGCTCAATGGCACCAATGGTTCCGTGGAGGCCGATGGCATAGTCTGGAACTCTC TGGACGTCTACCTCAATGACCCAGCCAATTACCTACTCAAGAATGGCACCACGGATGTGTTGCTGTGTGG GAACAGCTCTGATGCCGGGACATGTCCTGAAGGCTACCGGTGCCTGAAGGCAGGTGAGAACCCTGACCAC GGTTACACCAGCTTCGACTCCTTCGCCTGGGCTTTCCTTGCGCTCTTCCGCCTGATGACACAGGACTGCT GGGAACGTCTATACCAGCAGACCCTGAGGTCCGCAGGAAAGATCTACATGATCTTCTTCATGCTTGTCAT ATTCCTGGGCTCCTTCTACCTTGTGAACTTGATCCTGGCTGTGGTGGCCATGGCTTACGAGGAGCAAAAC CAAGCCACCATCGCCGAGACGGAAGAGAAGGAGAAGCGTTTCCAGGAGGCCATGGAGATGCTCAAGAAGG AACACGAGGCTCTCACCATCAGGGGCGTGGATACTGTGTCCCGTAGCTCTCTGGAGATGTCTCCTTTGGC CCCAGTAACCAACCATGAGAGAAGGAGCAAGAGGAGGAAACGACTGTCTTCAGGGACAGAGGATGGCGGA GATGACAGGCTCCCCAAGTCGGACTCAGAAGACGGTCCCAGAGCATTGAATCAGCTCAGCCTCACCCATG GGCTCAGCCGGACATCCATGAGGCCCCGCTCCAGCCGAGGGAGCATTTTCACATTCCGGCGACGGGACCA AGGCTCCGAGGCAGATTTTGCAGATGACGAGAACAGTACAGCAGGAGAGAGCGAGAGCCACCGCACATCG CTGCTGGTGCCCTGGCCCCTGCGCCGTCCCAGCACCCAAGGGCAGCCCGGCTTTGGAACCTCAGCTCCTG GTCATGTCCTCAATGGCAAAAGAAACAGCACCGTGGACTGCAATGGGGTGGTTTCCTTGCTGGGGGCAGG GGACGCAGAGGCCACCTCTCCAGGGAGCCACCTTCTCCGCCCTATAGTGCTGGACAGACCCCCAGACACG ACCACGCCGTCAGAGGAGCCGGGTGGGCCCCAGATGCTGACACCGCAGGCTCCATGTGCAGATGGCTTTG AGGAGCCCGGGGCACGGCAACGGGCACTCAGCGCCGTCAGTGTCCTCACCAGCGCTCTGGAAGAGTTGGA GGAGTCCCACCGTAAGTGTCCACCATGCTGGAATCGCTTTGCCCAGCATTACCTCATCTGGGAGTGCTGC CCACTCTGGATGTCCATCAAGCAGAAGGTGAAGTTCGTGGTCATGGACCCGTTTGCTGACCTCACCATCA CCATGTGCATCGTGCTCAACACGCTCTTCATGGCTCTGGAGCACTACAACATGACAGCCGAGTTTGAGGA GATGCTGCAGGTCGGAAACTTGGTCTTCACGGGAATCTTCACGGCAGAGATGACCTTCAAGATCATCGCC CTTGACCCATACTACTACTTCCAGCAGGGCTGGAATATCTTCGACAGCATCATCGTCATCCTCAGCCTCA TGGAGCTGGGCCTGTCTCGCATGGGCAACCTGTCTGTGCTGCGTTCCTTCCGTCTGCTGCGGGTCTTCAA GCTGGCCAAATCATGGCCCACCCTGAACACACTCATCAAGATCATCGGGAACTCTGTTGGTGCCCTGGGG AACCTGACCCTGGTGCTGGCCATCATCGTCTTCATCTTCGCCGTGGTGGGTATGCAGCTCTTCGGCAAGA ACTACTCAGAGCTGAGGCACCGAATCAGCGACTCTGGCCTGCTGCCCCGCTGGCACATGATGGACTTCTT CCACGCCTTCCTCATCATCTTCCGCATCCTCTGTGGAGAGTGGATCGAGACCATGTGGGACTGCATGGAG GTGTCTGGGCAGTCGCTGTGCTTGCTGGTCTTCCTGCTGGTCATGGTCATTGGCAACCTTGTGGTCCTGA ATCTCTTCTTGGCCTTGCTGCTCAGCTCCTTCAGCGCAGACAACCTCACAGCCCCTGACGAGGATGGGGA GATGAACAACCTCCAGCTGGCCCTGGCTCGCATCCAAAGGGGCCTTCGCTTTGTCAAGCGGACCACCTGG GACTTCTGCTGCGGGCTCCTGCGGCGGCGACCTAAGAAGCCCGCGGCTCTTGCCACCCACAGCCAGCTGC CCAGCTGTATCGCCGCTCCCAGGTCCCCACCACCCCCAGAGGTGGAGAAGGCGCCCCCAGCCCGCAAGGA AACACGGTTCGAGGAAGACAAGCGGCCGGGCCAGGGCACCCCTGGGGATACCGAGCCTGTGTGTGTTCCC ATCGCAGTGGCTGAGTCAGACACTGATGACCAGGAAGAGGATGAGGAGAACAGCCTTGGCACGGAGGAGG AAGAGTCCAGCAAACAGCAGGAATCCCAAGTTGTGTCTGGTGGCCACGAGCCCCCCCAGGAGCCCAGGGC CTGGAGCCAGGTGTCAGAGACCACATCCTCTGAAGCCGAGGCCAGTACGTCTCAGGCAGACTGGCAGCAA GAGCGGGAAGCGGAGCCCCGGGCCCCGGGGTGCGGTGAGACTCCCGAGGACAGTTACTCCGAGGGTAGCA CCGCTGACATGACCAACACCGCAGACCTCCTGGAGCAAATCCCAGACCTCGGGGAGGACGTCAAGGACCC AGAGGACTGCTTCACTGAAGGCTGCGTCCGACGTTGTCCTTGCTGCATGGTGGACACGACGCAGGCCCCA GGGAAGGTCTGGTGGAGACTGCGCAAGACCTGCTACCGCATAGTGGAGCACAGCTGGTTCGAGACTTTCA TCATCTTCATGATCCTGCTCAGCAGCGGAGCGCTGGCCTTCGAGGACATCTACCTGGAGGAGCGGAAGAC CATCAAGGTTCTGCTGGAGTACGCCGACAAGATGTTCACCTATGTCTTTGTGTTGGAGATGCTGCTCAAG TGGGTGGCCTACGGCTTCAAGAAGTACTTCACCAACGCCTGGTGCTGGCTGGACTTCCTAATTGTGGACG TCTCACTGGTCAGCCTCGTGGCCAACACCTTAGGCTTTGCCGAGATGGGTCCCATCAAGTCCCTGAGGAC ACTGCGTGCACTTCGACCCCTGAGGGCTTTGTCACGATTCGAGGGCATGAGGGTGGTGGTCAACGCGCTG GTGGGCGCCATCCCCTCCATCATGAATGTCCTCCTCGTCTGCCTCATCTTCTGGCTCATCTTCAGCATCA TGGGCGTGAACCTCTTCGCCGGGAAGTTCGGTAGGTGCATCAACCAGACCGAAGGGGACCTGCCTCTGAA CTACACCATTGTGAACAACAAGAGCGAGTGTGAGTCCTTCAACGTGACTGGAGAGTTGTACTGGACCAAG GTGAAAGTCAACTTTGACAACGTGGGGGCTGGGTACCTGGCCCTGCTGCAGGTGGCGACATTTAAAGGCT GGATGGACATCATGTATGCGGCTGTGGACTCCAGAGGGTATGAAGAGCAGCCGCAGTGGGAGGACAACCT CTACATGTACATCTACTTCGTCGTCTTCATCATCTTCGGCTCCTTCTTCACCCTCAACCTGTTTATTGGT GTCATCATTGATAACTTCAACCAGCAGAAGAAAAAGTTAGGGGGCCAGGACATCTTCATGACGGAGGAGC AGAAGAAGTACTACAATGCCATGAAGAAGCTGGGCTCCAAGAAACCCCAGAAGCCCATCCCACGGCCTCT GAACAAGTACCAGGGTTTCATATTCGACATTGTGACCAAGCAGGCCTTCGATGTTACCATCATGTTCCTC ATCTGTTTAAACATGGTGACCATGATGGTAGAGACAGATGATCAGAGCCCTGAAAAAGTCAACATCTTGG CCAAGATCAACCTGCTCTTCGTGGCCATCTTCACAGGAGAGTGTATTGTCAAGATGGCTGCCCTGCGCCA CTATTACTTCACCAACAGCTGGAACATCTTCGATTTCGTGGTTGTCATCCTCTCCATCGTTGGCACTGTC CTCTCCGACATCATCCAGAAGTACTTCTTCTCCCCAACGCTCTTCCGTGTCATCCGTCTGGCCAGGATCG GCCGCATCCTCAGGCTGATCCGAGGAGCCAAGGGGATTCGCACGTTGCTCTTCGCCCTCATGATGTCCCT GCCCGCCCTCTTCAACATCGGCCTCCTCCTCTTCCTCGTCATGTTCATCTACTCCATCTTCGGCATGGCC AACTTTGCCTACGTCAAGTGGGAGGCTGGCATCGACGACATGTTCAACTTCCAGACCTTTGCCAACAGCA TGCTGTGCCTTTTCCAGATCACCACGTCGGCTGGCTGGGATGGCCTCCTCAGCCCCATCCTCAACACAGG ACCCCCCTACTGTGACCCCAACCTGCCCAACAGCAATGGCTCCAGGGGGAACTGTGGGAGCCCAGCAGTG GGCATCCTCTTCTTCACCACCTACATCATCATCTCCTTCCTCATCGTGGTCAACATGTACATTGCCATCA TCCTGGAGAACTTCAGTGTGGCCACAGAAGAGAGCACAGAGCCCCTGAGCGAGGATGACTTCGACATGTT CTATGAGATCTGGGAGAAGTTCGACCCGGAGGCCACCCAGTTCATTGAGTATTTGGCCCTGTCCGACTTT GCCGATGCCCTGTCTGAGCCACTCCGCATCGCCAAGCCCAACCAGATAAGCCTCATCAATATGGACCTGC CCATGGTGAGCGGGGACCGCATCCACTGTATGGACATCCTGTTCGCCTTCACCAAGAGGGTGCTCGGGGA GTCTGGGGAGATGGATGCCCTGAAGATCCAGATGGAGGAGAAATTCATGGCGGCCAATCCTTCCAAGATC TCCTATGAGCCCATCACCACCACCCTGCGGCGAAAACATGAGGAGGTGTCGGCCACGGTCATCCAGCGGG CCTTCCGGAGGCACCTCCTGCAGCGCTCGGTGAAGCATGCTTCCTTCCTCTTCCGCCAGCAAGCGGGCAG CAGTGGCCTCTCTGATGAGGATGCCCCCGAGCGAGAGGGACTCATTGCCTACATGATGAATGAGAACTTC TCCCGGCGCAGCGGTCCCCTCTCCAGCTCTTCCATCTCCTCCACGTCCTTCCCACCGTCCTATGACAGCG TCACGAGGGCCACCAGTGATAACCTCCCAGTGCGTGCGTCGGACTACAGCCGCAGCGAAGATCTTGCAGA CTTCCCTCCATCTCCAGATAGGGACCGAGAGTCTATAGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222902 representing NM_021544 Red=Cloning site Green=Tags(s) MANFLLPRGTSSFRRFTRESLAAIEKRMAEKQARGSATSQESREGLPEEEAPRPQLDLQASKKLPDLYGN PPRELIGEPLEDLDPFYSTQKTFIVLNKGKTIFRFSATNALYVLSPFHPVRRAAVKILVHSLFSMLIMCT ILTNCVFMAQHDPPPWTKYVEYTFTAIYTFESLVKILARGFCLHAFTFLRDPWNWLDFSVIVMAYVSENI KLGNLSALRTFRVLRALKTISVIPGLKTIVGALIQSVKKLADVMVLTVFCLSVFALIGLQLFMGNLRHKC VRNFTELNGTNGSVEADGIVWNSLDVYLNDPANYLLKNGTTDVLLCGNSSDAGTCPEGYRCLKAGENPDH GYTSFDSFAWAFLALFRLMTQDCWERLYQQTLRSAGKIYMIFFMLVIFLGSFYLVNLILAVVAMAYEEQN QATIAETEEKEKRFQEAMEMLKKEHEALTIRGVDTVSRSSLEMSPLAPVTNHERRSKRRKRLSSGTEDGG DDRLPKSDSEDGPRALNQLSLTHGLSRTSMRPRSSRGSIFTFRRRDQGSEADFADDENSTAGESESHRTS LLVPWPLRRPSTQGQPGFGTSAPGHVLNGKRNSTVDCNGVVSLLGAGDAEATSPGSHLLRPIVLDRPPDT TTPSEEPGGPQMLTPQAPCADGFEEPGARQRALSAVSVLTSALEELEESHRKCPPCWNRFAQHYLIWECC PLWMSIKQKVKFVVMDPFADLTITMCIVLNTLFMALEHYNMTAEFEEMLQVGNLVFTGIFTAEMTFKIIA LDPYYYFQQGWNIFDSIIVILSLMELGLSRMGNLSVLRSFRLLRVFKLAKSWPTLNTLIKIIGNSVGALG NLTLVLAIIVFIFAVVGMQLFGKNYSELRHRISDSGLLPRWHMMDFFHAFLIIFRILCGEWIETMWDCME VSGQSLCLLVFLLVMVIGNLVVLNLFLALLLSSFSADNLTAPDEDGEMNNLQLALARIQRGLRFVKRTTW DFCCGLLRRRPKKPAALATHSQLPSCIAAPRSPPPPEVEKAPPARKETRFEEDKRPGQGTPGDTEPVCVP IAVAESDTDDQEEDEENSLGTEEEESSKQQESQVVSGGHEPPQEPRAWSQVSETTSSEAEASTSQADWQQ EREAEPRAPGCGETPEDSYSEGSTADMTNTADLLEQIPDLGEDVKDPEDCFTEGCVRRCPCCMVDTTQAP GKVWWRLRKTCYRIVEHSWFETFIIFMILLSSGALAFEDIYLEERKTIKVLLEYADKMFTYVFVLEMLLK WVAYGFKKYFTNAWCWLDFLIVDVSLVSLVANTLGFAEMGPIKSLRTLRALRPLRALSRFEGMRVVVNAL VGAIPSIMNVLLVCLIFWLIFSIMGVNLFAGKFGRCINQTEGDLPLNYTIVNNKSECESFNVTGELYWTK VKVNFDNVGAGYLALLQVATFKGWMDIMYAAVDSRGYEEQPQWEDNLYMYIYFVVFIIFGSFFTLNLFIG VIIDNFNQQKKKLGGQDIFMTEEQKKYYNAMKKLGSKKPQKPIPRPLNKYQGFIFDIVTKQAFDVTIMFL ICLNMVTMMVETDDQSPEKVNILAKINLLFVAIFTGECIVKMAALRHYYFTNSWNIFDFVVVILSIVGTV LSDIIQKYFFSPTLFRVIRLARIGRILRLIRGAKGIRTLLFALMMSLPALFNIGLLLFLVMFIYSIFGMA NFAYVKWEAGIDDMFNFQTFANSMLCLFQITTSAGWDGLLSPILNTGPPYCDPNLPNSNGSRGNCGSPAV GILFFTTYIIISFLIVVNMYIAIILENFSVATEESTEPLSEDDFDMFYEIWEKFDPEATQFIEYLALSDF ADALSEPLRIAKPNQISLINMDLPMVSGDRIHCMDILFAFTKRVLGESGEMDALKIQMEEKFMAANPSKI SYEPITTTLRRKHEEVSATVIQRAFRRHLLQRSVKHASFLFRQQAGSSGLSDEDAPEREGLIAYMMNENF SRRSGPLSSSSISSTSFPPSYDSVTRATSDNLPVRASDYSRSEDLADFPPSPDRDRESIV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_021544
ORF Size	6060 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_021544.4, NP_067519.2
RefSeq Size	8452 bp
RefSeq ORF	6063 bp
Locus ID	20271
UniProt ID	Q9JJV9
MW	228.2 kDa
Gene Summary	This protein mediates the voltage-dependent sodium ion permeability of excitable membranes. Assuming opened or closed conformations in response to the voltage difference across the membrane, the protein forms a sodium-selective channel through which Na(+) ions may pass in accordance with their electrochemical gradient (PubMed:11834499, PubMed:23420830). It is a tetrodotoxin-resistant Na(+) channel isoform. This channel is responsible for the initial upstroke of the action potential. Channel inactivation is regulated by intracellular calcium levels (By similarity).[UniProtKB/Swiss-Prot Function]

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