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Rnf13 (NM_011883) Mouse Tagged ORF Clone
CAT＃: MR222794
- TrueORF®
Rnf13 (Myc-DDK-tagged) - Mouse ring finger protein 13 (Rnf13), transcript variant 2
ORF Plasmid: tGFP
"NM_011883" in other vectors (4)
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CNY 2,660.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2010001H16Rik; Rz; Rzf |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222794 representing NM_011883
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCTCTCCATTGGGATGCTCATGCTGTCCGCCACACAGGTCTACACCATCTTGACTGTGCAGCTCT TTGCATTCCTAAACCTGCTGCCTGTAGAAGCAGACATTTTAGCATATAATTTTGAAAATGCATCTCAGAC ATTTGAGGACCTTCCAGCAAGATTTGGTTATAGACTACCAGCTGAAGGCTTAAAGGGTTTTCTGATTAAC TCAAAACCAGAGAATGCGTGTGAACCCATAGTGCCTCCACCACTGAAAGACAATTCTTCCGGCACGTTCA TCGTGCTGATTAGAAGACTTGATTGCAATTTTGATATAAAGGTTTTAAATGCACAGAGAGCAGGATACAA AGCAGCCATAGTTCACAATGTAGACTCTGATGACCTGATTAGCATGGGATCCAACGACATTGATACACTA AAGAAAATTGACATTCCGTCTGTCTTTATTGGTGAATCATCAGCTAATTCCCTGAAAGATGAATTCACAT ATGAAAAAGGGGGCCACATTATCTTAGTTCCAGAACTGAGTCTTCCTTTGGAGTACTATCTGATCCCCTT CCTCATCATAGTGGGCATCTGCCTCATCTTAATAGTCATTTTCATGATCACAAAATTTGTTCAGGACAGA CATAGAAACAGAAGAAATAGACTTCGTAAAGATCAACTTAAAAAACTTCCCGTACATAAATTTAAAAAGG GAGATGAGTACGATGTGTGTGCTATCTGTCTGGAGGAGTATGAAGACGGAGATAAGCTAAGGATCCTCCC CTGCTCCCATGGTATGAGTACTCACACAGTGCTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222794 representing NM_011883
Red=Cloning site Green=Tags(s) MLLSIGMLMLSATQVYTILTVQLFAFLNLLPVEADILAYNFENASQTFEDLPARFGYRLPAEGLKGFLIN SKPENACEPIVPPPLKDNSSGTFIVLIRRLDCNFDIKVLNAQRAGYKAAIVHNVDSDDLISMGSNDIDTL KKIDIPSVFIGESSANSLKDEFTYEKGGHIILVPELSLPLEYYLIPFLIIVGICLILIVIFMITKFVQDR HRNRRNRLRKDQLKKLPVHKFKKGDEYDVCAICLEEYEDGDKLRILPCSHGMSTHTVL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_011883 |
| ORF Size | 804 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_011883.5 |
| RefSeq Size | 1116 bp |
| RefSeq ORF | 807 bp |
| Locus ID | 24017 |
| UniProt ID | O54965 |
| MW | 30.7 kDa |
| Gene Summary | This gene encodes a member of the PA-TM-RING family of proteins that contain a protease associated (PA) domain and a RING finger domain separated by a transmembrane (TM) domain. The encoded protein is an E3 ubiquitin ligase localized to the endosomal-lysosomal vesicles and inner nuclear membrane. Mice lacking the encoded protein have impaired learning abilities associated with a decreased synaptic vesicle density and dysregulated SNARE complex assembly. Alternative splicing of this gene results in multiple transcript variants. A pseudogene for this gene has been identified on the X chromosome. [provided by RefSeq, Jan 2015] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209692 | Rnf13 (untagged) - Mouse ring finger protein 13 (Rnf13), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG222794 | Rnf13 (tGFP-tagged) - Mouse ring finger protein 13 (Rnf13) transcript variant 2, (10ug) |
CNY 2,950.00 |
|
| MR222794L3 | Lenti ORF clone of Rnf13 (Myc-DDK-tagged) - Mouse ring finger protein 13 (Rnf13), transcript variant 2 |
CNY 4,560.00 |
|
| MR222794L4 | Lenti ORF clone of Rnf13 (mGFP-tagged) - Mouse ring finger protein 13 (Rnf13), transcript variant 2 |
CNY 4,560.00 |
