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Adgra2 (NM_054044) Mouse Tagged ORF Clone
CAT＃: MR222754
- TrueORF®
Gpr124 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 124 (Gpr124)
ORF Plasmid: tGFP
"NM_054044" in other vectors (4)
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CNY 9,264.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 8430414O08Rik; 9530074E10Rik; Gpr124; mKIAA1531; Tem5 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222754 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCCGGTGCCTCCCGCGCGTTTGCTGCTGCTGCCGCTGCTGCCTTGTCTTCTGCTCCTGGCTCCTGGAA CTCGGGGTGCGCCTGGCTGCCCGGTCCCTATCCGCGGTTGCAAGTGCTCTGGGGAGCGGCCCAAGGGACT AAGTGGCGGCGCCCACAACCCGGCTCGAAGGAGGGTGGTGTGCGGCGGTGGGGATCTCCCCGAACCTCCA GATCCCGGCCTTCTGCCAAACGGCACCATCACCTTGCTCTTGAGCAACAACAAGATTACTGGGCTCCGCA ATGGATCCTTCTTGGGACTGTCCCTGTTGGAGAAGTTGGACCTGAGGAGCAATGTCATCAGCACTGTGCA GCCTGGAGCCTTCCTAGGTCTGGGAGAGCTAAAACGCTTAGATCTCTCCAACAATCGGATTGGCTGTCTC ACCTCTGAGACATTTCAAGGGCTCCCTAGACTTCTCAGACTAAACATATCTGGAAACATCTACTCTAGTC TGCAACCTGGGGTCTTTGATGAGCTGCCAGCCCTTAAGATTGTGGACTTTGGTACTGAGTTTCTGACCTG TGACTGCCGCCTGCGCTGGCTGCTGCCCTGGGCCCGGAATCACTCCCTGCAGCTGTCTGAGCGCACACTC TGTGCCTACCCCAGTGCCCTGCACGCCCATGCCCTGAGCAGCCTCCAGGAGTCCCAGCTTCGCTGTGAAG GGGCCCTGGAACTGCACACCCACTACCTCATCCCATCCCTTCGCCAAGTGGTGTTCCAGGGTGACCGCCT GCCCTTCCAGTGCTCAGCCAGCTACTTGGGCAACGATACCCGGATCCACTGGTACCACAATGGGGCTCCT ATGGAGAGCGATGAGCAGGCCGGCATCGTCCTTGCTGAAAACCTTATCCATGATTGCACCTTCATCACCA GTGAGCTGACCCTGTCTCACATTGGTGTGTGGGCCTCTGGTGAATGGGAGTGCTCCGTGTCCACAGTCCA AGGCAACACCAGCAAGAAGGTGGAGATAGTAGTGCTGGAGACCTCTGCCTCCTACTGCCCTGCAGAGCGG GTGACCAACAACCGTGGGGACTTCAGGTGGCCCCGAACCTTGGCTGGCATCACGGCTTACCAGTCCTGTT TACAGTACCCCTTCACCTCTGTGCCCTTGAGTGGGGGAGCCCCGGGTACCCGAGCCTCACGCAGGTGTGA CCGAGCTGGCCGCTGGGAGCCAGGGGACTACTCGCACTGTCTGTACACCAATGACATCACTCGGGTGCTC TACACCTTTGTGCTGATGCCCATCAACGCCTCCAATGCATTGACGTTGGCCCACCAGCTGCGAGTGTATA CCGCAGAGGCCGCCAGCTTCTCAGACATGATGGACGTGGTCTATGTGGCTCAGATGATCCAGAAGTTTTT GGGTTACGTTGACCAGATCAAGGAGCTGGTGGAGGTGATGGTGGACATGGCCAGCAACCTGATGCTGGTG GATGAGCACCTTCTGTGGCTGGCCCAGAGAGAAGACAAAGCCTGCAGTGGCATTGTGGGTGCCCTGGAGC GAATCGGAGGAGCTGCTCTTAGCCCCCATGCCCAGCACATCTCTGTGAATTCAAGAAATGTGGCACTGGA GGCCTACCTCATCAAGCCTCACAGCTACGTGGGTCTGACTTGCACGGCTTTCCAGAGGAGGGAGGTAGGA GTGTCGGGTGCACAGCCAAGCAGCGTCGGCCAGGATGCCCCAGTGGAGCCCGAACCCCTAGCTGATCAGC AGCTTAGGTTCCGCTGCACCACTGGGAGGCCCAACATTTCTCTGTCATCCTTCCACATCAAGAATAGCGT GGCCCTGGCCTCCATCCAGCTGCCCCCCAGCCTGTTCTCAACCCTTCCGGCTGCCCTGGCTCCCCCAGTC CCTCCAGATTGCACCCTGCAACTGCTGGTCTTCAGAAATGGCCGTCTTTTCCGCAGCCACGGCAACAACA CTTCCCGTCCTGGAGCAGCTGGGCCTGGCAAGAGGCGTGGTGTGGCCACCCCAGTCATATTTGCAGGAAC CAGTGGCTGTGGTGTGGGAAACTTGACGGAGCCCGTGGCTGTGTCACTGAGGCACTGGGCTGAAGGAGCT GACCCCATGGCAGCTTGGTGGAACCAGGACGGGCCTGGAGGCTGGAGTTCTGAAGGCTGCAGGCTCCGCT ACAGCCAGCCCAACGTCAGCTCCCTGTACTGCCAGCACTTGGGCAATGTGGCCGTGCTTATGGAGCTGAA TGCATTCCCGCGGGAGGCAGGAGGCTCTGGGGCTGGGTTGCATCCCGTCGTGTACCCCTGCACGGCTTTG CTGCTACTCTGTCTCTTCTCCACCATCATCACGTACATCCTCAACCACAGCTCCATCCACGTGTCCCGGA AGGGTTGGCACATGCTGCTGAACCTTTGTTTCCATATGGCCATGACCTCTGCTGTCTTCGTGGGGGGCGT CACGCTCACCAACTACCAAATGGTTTGTCAAGCGGTGGGCATCACTCTGCACTACTCTTCCTTGTCGTCA CTGCTCTGGATGGGGGTCAAGGCTCGAGTCCTCCACAAGGAGCTTAGTTGGAGGGCACCCCCTCTGGAAG AAGGGGAAGCAGCTCCGCCTGGTCCTCGCCCCATGCTCCGGTTCTACTTGATTGCTGGAGGGATCCCCCT CATAATCTGCGGCATCACCGCTGCGGTCAACATCCACAACTACCGGGACCACAGTCCCTATTGTTGGCTG GTGTGGCGTCCAAGCCTTGGTGCCTTCTACATACCGGTGGCGTTGATTCTGCCTATCACCTGGATCTACT TCTTGTGTGCAGGCCTTCACTTACGGAGCCATGTGGCCCAGAATCCAAAGCAGGGTAACAGGATCTCTCT GGAGCCAGGGGAAGAGCTGAGGGGTTCCACCAGGCTCAGGAGTAGTGGCGTCCTCCTGAATGACTCTGGT TCTCTTTTGGCTACAGTTAGCGCAGGAGTAGGGACACCTGCGCCCCCAGAGGATGGTGATGGCGTATATT CTCCTGGAGTCCAGCTGGGGGCGCTGATGACCACGCATTTCCTGTACCTGGCTATGTGGGCTTGTGGTGC CTTGGCGGTGTCTCAGCGCTGGCTGCCCCGAGTGGTGTGTAGCTGTCTGTACGGCGTGGCAGCTTCAGCT CTTGGTCTGTTTGTCTTCACTCACCACTGTGCCAGACGTAGAGATGTCCGGGCTTCCTGGCGCGCCTGCT GCCCTCCTGCTTCGCCCTCGGCCTCCCATGTCCCAGCCCGGGCCCTGCCGACTGCTACAGAGGATGGATC CCCAGTGTTGGGGGAGGGACCAGCCTCTCTCAAGTCCTCCCCAAGTGGCAGCAGTGGCCGCGCGCCGCCA CCCCCCTGCAAACTCACCAATCTGCAGGTGGCCCAGAGTCAGGTGTGCGAGGCAAGCGTGGCCGCCCGCG GAGATGGAGAGCCAGAGCCCACCGGCTCCCGTGGCAGCCTAGCTCCCCGGCACCATAACAACCTGCATCA TGGGCGCCGAGTACACAAGAGTCGGGCCAAGGGGCACCGAGCCGGAGAGACTGGTGGTAAGAGCCGGCTC AAGGCGTTGCGCGCGGGCACGTCCCCAGGAGCTCCCGAGCTGTTGTCCAGTGAGAGCGGCAGTTTGCACA ACAGCCCGTCTGACAGCTACCCGGGCAGCAGCCGCAACAGTCCAGGCGACGGTCTTCCACTCGAGGGTGA GCCCATGCTTACGCCGTCGGAGGGCAGTGACACAAGCGCCGCGCCAATCGCTGAGACGGGGCGCCCCGGG CAGCGCCGCAGCGCCAGCCGTGACAACCTCAAGGGCAGCGGCAGCGCGCTGGAGAGGGAGAGCAAGCGCC GCTCCTATCCGCTCAACACTACCAGTCTTAACGGTGCCCCCAAAGGGGGCAAGTATGAGGATGCCAGTGT AACTGGTGCAGAGGCCATAGCGGGAGGCTCCATGAAGACTGGCCTCTGGAAAAGCGAGACCACCGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222754 protein sequence
Red=Cloning site Green=Tags(s) MPVPPARLLLLPLLPCLLLLAPGTRGAPGCPVPIRGCKCSGERPKGLSGGAHNPARRRVVCGGGDLPEPP DPGLLPNGTITLLLSNNKITGLRNGSFLGLSLLEKLDLRSNVISTVQPGAFLGLGELKRLDLSNNRIGCL TSETFQGLPRLLRLNISGNIYSSLQPGVFDELPALKIVDFGTEFLTCDCRLRWLLPWARNHSLQLSERTL CAYPSALHAHALSSLQESQLRCEGALELHTHYLIPSLRQVVFQGDRLPFQCSASYLGNDTRIHWYHNGAP MESDEQAGIVLAENLIHDCTFITSELTLSHIGVWASGEWECSVSTVQGNTSKKVEIVVLETSASYCPAER VTNNRGDFRWPRTLAGITAYQSCLQYPFTSVPLSGGAPGTRASRRCDRAGRWEPGDYSHCLYTNDITRVL YTFVLMPINASNALTLAHQLRVYTAEAASFSDMMDVVYVAQMIQKFLGYVDQIKELVEVMVDMASNLMLV DEHLLWLAQREDKACSGIVGALERIGGAALSPHAQHISVNSRNVALEAYLIKPHSYVGLTCTAFQRREVG VSGAQPSSVGQDAPVEPEPLADQQLRFRCTTGRPNISLSSFHIKNSVALASIQLPPSLFSTLPAALAPPV PPDCTLQLLVFRNGRLFRSHGNNTSRPGAAGPGKRRGVATPVIFAGTSGCGVGNLTEPVAVSLRHWAEGA DPMAAWWNQDGPGGWSSEGCRLRYSQPNVSSLYCQHLGNVAVLMELNAFPREAGGSGAGLHPVVYPCTAL LLLCLFSTIITYILNHSSIHVSRKGWHMLLNLCFHMAMTSAVFVGGVTLTNYQMVCQAVGITLHYSSLSS LLWMGVKARVLHKELSWRAPPLEEGEAAPPGPRPMLRFYLIAGGIPLIICGITAAVNIHNYRDHSPYCWL VWRPSLGAFYIPVALILPITWIYFLCAGLHLRSHVAQNPKQGNRISLEPGEELRGSTRLRSSGVLLNDSG SLLATVSAGVGTPAPPEDGDGVYSPGVQLGALMTTHFLYLAMWACGALAVSQRWLPRVVCSCLYGVAASA LGLFVFTHHCARRRDVRASWRACCPPASPSASHVPARALPTATEDGSPVLGEGPASLKSSPSGSSGRAPP PPCKLTNLQVAQSQVCEASVAARGDGEPEPTGSRGSLAPRHHNNLHHGRRVHKSRAKGHRAGETGGKSRL KALRAGTSPGAPELLSSESGSLHNSPSDSYPGSSRNSPGDGLPLEGEPMLTPSEGSDTSAAPIAETGRPG QRRSASRDNLKGSGSALERESKRRSYPLNTTSLNGAPKGGKYEDASVTGAEAIAGGSMKTGLWKSETTV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_054044 |
| ORF Size | 3990 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_054044.2, NP_473385.2 |
| RefSeq Size | 5520 bp |
| RefSeq ORF | 4011 bp |
| Locus ID | 78560 |
| UniProt ID | Q91ZV8 |
| MW | 142.5 kDa |
| Gene Summary | Endothelial receptor which functions together with RECK to enable brain endothelial cells to selectively respond to Wnt7 signals (WNT7A or WNT7B) (PubMed:25373781, PubMed:25558062, PubMed:28803732). Plays a key role in Wnt7-specific responses, such as endothelial cell sprouting and migration in the forebrain and neural tube, and establishment of the blood-brain barrier (PubMed:21071672, PubMed:21282641, PubMed:21421844, PubMed:25373781, PubMed:28288111). Acts as a Wnt7-specific coactivator of canonical Wnt signaling: required to deliver RECK-bound Wnt7 to frizzled by assembling a higher-order RECK-ADGRA2-Fzd-LRP5-LRP6 complex (By similarity). ADGRA2-tethering function does not rely on its G-protein coupled receptor (GPCR) structure but instead on its combined capacity to interact with RECK extracellularly and recruit the Dishevelled scaffolding protein intracellularly (By similarity). Binds to the glycosaminoglycans heparin, heparin sulfate, chondroitin sulfate and dermatan sulfate (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224269 | Gpr124 (untagged) - Mouse G protein-coupled receptor 124 (Gpr124), (10ug) |
CNY 17,100.00 |
|
| MG222754 | Gpr124 (tGFP-tagged) - Mouse G protein-coupled receptor 124 (Gpr124), (10ug) |
CNY 11,880.00 |
|
| MR222754L3 | Lenti ORF clone of Gpr124 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 124 (Gpr124) |
CNY 12,730.00 |
|
| MR222754L4 | Lenti ORF clone of Gpr124 (mGFP-tagged) - Mouse G protein-coupled receptor 124 (Gpr124) |
CNY 12,730.00 |
