Plxnb1 (NM_172775) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	2900002G15Rik; AU042020; mKIAA0407
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR222673 representing NM_172775 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGTCCTCGGCCCAGTTCTTCTCCAGGTGTTCTGGGCCGGGTGTGTCGTCACCCTGCGGTCCCCTC TGCCAGCTGCTTTCACTGCCAATGGCACACATCTACAACACTTGGCAAGGGACCCCACCACAGGTACCCT CTATGTAGGGGCCACCAACTTCCTGTTCCAGTTGAGCCCTGGGCTGCAGCTGGAAGCCGTGGTGTCCACG GGCCCTGTGAATGACAGCCGGGATTGCCTGCCACCTGTGATACCTGATGAATGTCCCCAAGCCCAGCCTA CTAACAACCCTAACCAGCTGCTCCTGGTGAGCCCAGAGGCTCTGGTGGTGTGTGGGAGCATACACCAGGG CATCTGTGAGCTACGGAGCCTGGGACAGATCAGGCAGCTGCTGCTACGGCCAGAGCGACCTGGGGACACC CAGTATGTGGCTGCAAATGACCCTGCAGTCAGTACAGTGGGGCTGGTGGCCCAGGGATTGGTAGGGGAGC CCCTCCTGTTTGTGGGGCGGGGATACACCAGCAGGGGTGTAGGTGGTGGGATTCCTCCCATTACAACCCG AGCCCTGCGACCACCGGACCCCCAAGCTGCCTTCTCTTATGAAGAAACAGCCAAGTTAGCAGTGGGCCGC CTGTCCGAGTACAGCCACCACTTCGTGAGTGCCTTTGTACGCGGGGCCAGTGCATACTTCCTGTTCTTGC GGCGAGACCTGAAGGCCCCTTCTAGAGCTTTCCGTGCCTATGTGTCTCGAGTGTGCCTTCAGGACCAGCA CTACTACTCTTATGTGGAATTGCCCCTGGCCTGCCAGGGTGGTCGTTACGGTCTTATCCAGGCTGCAGCT GTAGCCACGTCCAAGGAGGTGGCCCGTGGGGACGTACTCTTTGCAGCTTTCTCCTCAGTGGCTCCTCCCA CTGTGGATTGGCCCCTGTCAGCATCTACTGGGGCATCTGGAACCTCTGTGCTCTGTGCCTTCCCCCTGGA TGAGGTAGACCAGCTTGCTAATTACACTCGAGATGCCTGTTATACTCGGGAAGGCCGTGCTGAGAACGGG ACCAAGGTTGCTGACATTGCATACGATGTCCTTTCCGACTGTGCGCAGCTACCAGTGGACACCCCGGATG CTTTTCCATGTGGCTCTGACCACACACCCAGTCCCATGGTCAGCTGTGTCCCTTTGGAAGCCACGCCAAT TCTGGAGCTACCAGGGGTTCAGCTAACAGCTGTGGCTGTCACCATGGAAGATGGACACACTATTGCTTTC CTGGGTGACAGTCAAGGACAGTTGCATAGGGTCTACTTAGGCCCTGGAAGAAGTGCTGCCCCATATTCTA AACAGAGCATCCAGCCGGGGTCTCCTGTGAACAGAGATCTTACCTTTGATGGTACCTTTGAGCATCTCTA TGTAGCAACTCAGACTACTCTTGTGAAGGTTCCTGTGGCTCCTTGTGCTCAGCATCTGGACTGTGACTCT TGCCTTGCTCACAGGGACCCTTATTGCGGATGGTGTGTGCTCCTGGGCAGGTGTAGTCGCCGGTCGGAGT GCTCAAGGGACCAGGGCCCAGAGCAGTGGCTGTGGAGCTTCCAGCCGGAACTGGGCTGTCTTCGAGTGGT GGCCGTGAGCCCTGCCAATATCAGTCGGGAAGAGAGGAGGGAGGTTTTCTTGTCAGTGCCAGGCCTGCCA TCTCTCTGGCCAGGGGAGTCATATTTCTGCTACTTTGGAGACCAACAGAGTCCTGCTCTACTGACCAGTT CTGGTGTGATGTGTCCCTCCCCAGACCCCAGTGAGGCTCCAGTGCTGCAGAGAGGAGCCGACCATATCTC TGTGAACGTGGAGCTCAGGTTTGGTGCCGTGGTGATCGCCAGCACCTCCCTCTCCTTCTATGACTGCGTG GCAGTTACTGCGTCTTCCCCATCTGCACCGTGCCGGGCCTGTGTGAGCAGCCGCTGGGGCTGTAACTGGT GTGTGTGGCAGCAGCTGTGCACACACAAGGCCTCGTGTGACGCTGGGCCTATGGTGGCAAGCCAACAGAG CCCACTCCTTCCCCTAATCCCTCCTGCAAGGGATGAACTCACCCCTTTCCCACCCACAGTCCCCCAAACC ACGGTCACTCCTACCCCCAACAGCTTCCCAATAGAGCCTAGGGCTCCCTCCACAGCCTCAGATGTCCTAC CTGGGGCCAAGCCTTCCCGGCTCAGCCTCTGGGGCCCATGGGCAGGTCCTGGCCCCATACTTTCCCCTAC TTCCACAGAGTCACCTCTTCATGAGAAGCCCCTTCCTCCTGACCCCCCTACCATACCTGGAACCACTGTC CCTGCCCCCACTGGCTTGGGACCATCGACCACACCTGAGGACCTCTTGGCCTCCTACCCATTCCCCTCAG ATGCAGCTGCAGTGTCCCCTGCAGAGCCTGGCCCTGAGGCTCTGCCTTCCATGGTGGCTCTGGACCAGCC CCCTGGCACTGTTCCAGACACTACTTTCCCAGGGGCCCCTGGCTCCATGAAGCCCGTTCTGGATTGGCTC ACCAAAGGAGGCGGCGAGCTGCCCGAGGCGGATGAGTGGATGGGGGGTGACACGCCCGCCTTCTCCACTT CCACACTCCTCTCAGGTGATGGAGACTCAGCAGAGCACGAGGGCCCTCCTGCCCCCCTCATCCTCCTGTC CAGCCTCGACTACCAGTACGACACCCCCGGGCTCTGGGAGCTGGGAGAGGTGAATCAGAGGGTGAGCTCC TGCCCCTGTGTGGAGACCGTCCAGGGCTCCTTGCTGATACCGGTCCATGTGGAACGCGAAGTCCAGCTTC GAGGCAGGAACCTGTGGCTTTTCCAGGATGGCCCGAGGAGCAGCGAGTGTGTGCTGGAGCTAGGGAGTCG GGAGGTGGCTGTGGAGGCTCAGGTGGAGTGTGCGCCGCCTCCAGATGTCTGGTGCCACATCAAGTGCCAG CAGCATCAGTTCAGCTATGAAGCTTTGAAGCCAGAACTGCAGGTGGGGCTGTTCCTGCGTTGGGCAGGCG GTCTGCGCGTGGACAGTGCCGATGGGCTGCATGTGGTGTTGTATGACTGCTCTGTGGGACATGGGGACTG CAGCCGCTGCCAAACTGCCATGCCTCAGTACGACTGTGTGTGGTGTGAGGGGGAGCGTCCGCGTTGTGTG GCCCGGGAAGCCTGTAATGAAGCCGAGACTGTGGCCACTCAGTGCCCCGCACCTCTCATTCACTCGGTGG ATCCACTGACTGGACCTATAGATGGAGGCACCCGTGTCACTATCAGGGGCTCCAACCTGGGCCAACATGT GCAGGATGTCCTGGACATGGTCAGAGTGGCCGGAGTTCCCTGCGCTGTGGATGCTGGGGAGTATGATGTC TCTAGTAGTCTTGTGTGCATCACTGGAGCCAGCGGGGAGGAGGTGACTGGCACTGTGGCAGTGGAGGTGC CTGGAAGAGGACACGGTGTCTCAGAGTTCAGCTTTGCCTATCAGGATCCAAAAGTACACTCCATCTTCCC AGCCCGTGGCCCTAGAGCTGGAGGTACCCGCCTTACCCTGCATGGTTCTAAGCTCCTGACTGGACGGCTA GAGGACATCCGTGTGGTGGTTGGAGACCAGCCTTGCCACCTGCTCCTGGAGCAGCAGTCTGAGCAGCTAC ACTGTGAGACCGGCCCATACCCTGTGCCTGCTGAACTTCCAGTGACTGTCTTGTTTGGGGCCACTGAGCG GAGGCTTCAGCACGGCCAATTCAAGTATACATCAGACCCCAATGTCACCTCAGTGGGCCCCTCCAAGAGC TTCTTCAGCGGAGGACGTGAGATATGGGTCCGCGGCCAGGATCTTGATGTGGTACAGAGGCCAAGAATCC GAGTGACCGTGGTCCCAAGACAGCATGGCCAGGGGCTTGCACAGAAGCAACACGTGGTCCCTGAGAAATT TGAGGAGCCGTGTCTCGTGAACTCCTCCCACCTCCTCATGTGCCGCACTCCCGCTCTCCCTGGCCCACCC TGGGACTCTGGGGTCCAGGTGGAGTTTATCCTCGACAACATGGTCTTTGACTTTGCTGCACTGAGCCCCA CACCCTTCTCCTATGAGGCTGATCCCACCCTGCGTTCCCTGAACCCCGAGGATCCCAGCACGCCGTTCCG GCACAAGCCAGGGAGTGTGTTCTCTGTGGAGGGGGAGAATCTGGACCTCGCCATGTCTAAAGAAGAGGTG GTGGCCATGATAGGGGACGGGCCCTGCGTGGTAAAGACACTGACCCGGAACCACCTGTACTGTGAGCCCC CTGTGGAGCAGCCCCTGCCACATCCCCATGCCCTCCGAGAGGCTCCAGATGCTTTGCCTGAGTTCACGGT ACAGATGGGCAACCTGCGCTTCTCCTTGGGTCATGTGCAGTACGATGGCGAGAGCCCCGTGGCTTTTCCT GTGGCAGCCCAAGTGGGCTTGGGAGTGGGCACGTCTCTCCTGGCTCTGGGTGTCATCATCATTGTCCTCA TATACAGGAGGAAGAGCAAGCAGGCCCTGAGGGACTATAAGAAAGTGCAGATCCAGCTGGAGAACCTGGA GAGCAGTGTACGGGACCGCTGTAAGAAGGAGTTTACAGACCTCATGACGGAGATGACGGATCTCACCAGT GACCTCCTTGGCAGCGGTATCCCCTTCCTTGACTACAAAGTGTATGCTGAGAGGTTCTTCTTCCCTGGGT ACCGGGAGTCCCCCTTGCACAGGGACCTCGGTGTGCCTGACAGCAGGCGACCCACCGTGGAACAGGGCCT GGGGCAGCTCTCCAACCTGCTAAACAGCAAGCTCTTCCTTACCAAGTTCATCCACACACTGGAGAGTCAG CGCACCTTCTCTGCTCGGGACCGTGCCTACGTGGCATCTCTGCTCACTGTTGCACTTCACGGGAAGCTTG AATACTTCACGGACATACTGCGGACTCTGCTCAGTGACCTGGTAGCTCAGTATGTTGCCAAGAACCCCAA GCTGATGCTGCGCAGGACAGAGACCGTGGTAGAAAAGCTGCTCACCAACTGGATGTCCATCTGCCTCTAC ACCTTTGTGAGGGACTCTGTGGGAGAGCCTCTGTATATGCTCTTCAGAGGGATTAAGCATCAAGTGGACA AGGGTCCCGTGGACAGTGTGACTGGCAAAGCCAAATACACTCTGAATGACAACCGCCTGCTCAGAGAGGA TGTGGAGTACCGTCCCTTGACCTTGAATGCTCTTCTGGCTGTGGGGCCTGGGGCAGGAGAAGCCCAGTGT GTACCTGTGAAAGTCCTGGACTGTGACACCATCTCCCAGGCCAAGGAGAAGATGCTAGACCAGCTTTACA AGGGAGTGCCTCTTGCCCAGCGGCCCGACTCTTGCACCTTGGATGTTGAATGGCGGTCTGGAGTGGCTGG GCACCTTATCCTTTCTGATGAGGACGTCACTTCCGAACTCCAGGGTCTGTGGAGGCGTCTGAATACACTG CAACATTACAAGGTCCCAGATGGAGCAACGGTGGCCCTTGTCCCCTGCCTCACCAAGCATATTCTTAGGG AAAACCAGGATTATGTCCCTGGGGAACGGACCCCAATGCTGGAGGATGTAGATGAGGGGGGCATCCGGCC CTGGCACCTGGTAAAGCCGAGTGATGAACCAGAGCCTCCCAGGCCGAGGAGGGGCAGCCTTCGGGGTGGG GAGCGTGAGCGAGCCAAGGCTATCCCTGAGATCTACCTGACACGCCTGCTATCCATGAAGGGCACACTGC AGAAGTTTGTGGATGACCTGTTCCAGGTGATTCTCAGCACCAGCCGCCCTGTGCCTCTGGCTGTGAAGTA CTTCTTTGACTTGCTGGATGAACAAGCTCAGCAGCATGGCATCTCTGATCAGGATACTATCCACATCTGG AAGACCAACAGCCTGCCGCTAAGGTTCTGGATCAACATCATCAAGAACCCACAGTTTGTGTTCGATGTGC AGACTTCGGATAACATGGATGCTGTGCTCCTGGTCATTGCACAGACCTTCATGGATGCTTGCACCCTGGC CGACCACAAGCTGGGCCGGGATTCTCCCATCAACAAACTTCTGTATGCTCGAGATATTCCCCGTTACAAA CAGATGGTGGAAAGGTACTATGCAGACATCAGACAGACTGTCCCGGCCAGTGACCAAGAGATGAACTCAG TCTTGGCGGAGCTGTCCCGGAACTGCTCTGCCGACCTTGGGGCGCGAGTGGCTCTGCATGAACTCTACAA GTATATCAACAAGTACTATGACCAGATCATCACTGCCCTGGAGGAGGATGGCACTGCCCAGAAGATGCAG CTGGGCTACCGGCTCCAGCAGATCGCCGCTGCTGTGGAAAACAAGGTCACGGATCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR222673 representing NM_172775 Red=Cloning site Green=Tags(s) MSVLGPVLLQVFWAGCVVTLRSPLPAAFTANGTHLQHLARDPTTGTLYVGATNFLFQLSPGLQLEAVVST GPVNDSRDCLPPVIPDECPQAQPTNNPNQLLLVSPEALVVCGSIHQGICELRSLGQIRQLLLRPERPGDT QYVAANDPAVSTVGLVAQGLVGEPLLFVGRGYTSRGVGGGIPPITTRALRPPDPQAAFSYEETAKLAVGR LSEYSHHFVSAFVRGASAYFLFLRRDLKAPSRAFRAYVSRVCLQDQHYYSYVELPLACQGGRYGLIQAAA VATSKEVARGDVLFAAFSSVAPPTVDWPLSASTGASGTSVLCAFPLDEVDQLANYTRDACYTREGRAENG TKVADIAYDVLSDCAQLPVDTPDAFPCGSDHTPSPMVSCVPLEATPILELPGVQLTAVAVTMEDGHTIAF LGDSQGQLHRVYLGPGRSAAPYSKQSIQPGSPVNRDLTFDGTFEHLYVATQTTLVKVPVAPCAQHLDCDS CLAHRDPYCGWCVLLGRCSRRSECSRDQGPEQWLWSFQPELGCLRVVAVSPANISREERREVFLSVPGLP SLWPGESYFCYFGDQQSPALLTSSGVMCPSPDPSEAPVLQRGADHISVNVELRFGAVVIASTSLSFYDCV AVTASSPSAPCRACVSSRWGCNWCVWQQLCTHKASCDAGPMVASQQSPLLPLIPPARDELTPFPPTVPQT TVTPTPNSFPIEPRAPSTASDVLPGAKPSRLSLWGPWAGPGPILSPTSTESPLHEKPLPPDPPTIPGTTV PAPTGLGPSTTPEDLLASYPFPSDAAAVSPAEPGPEALPSMVALDQPPGTVPDTTFPGAPGSMKPVLDWL TKGGGELPEADEWMGGDTPAFSTSTLLSGDGDSAEHEGPPAPLILLSSLDYQYDTPGLWELGEVNQRVSS CPCVETVQGSLLIPVHVEREVQLRGRNLWLFQDGPRSSECVLELGSREVAVEAQVECAPPPDVWCHIKCQ QHQFSYEALKPELQVGLFLRWAGGLRVDSADGLHVVLYDCSVGHGDCSRCQTAMPQYDCVWCEGERPRCV AREACNEAETVATQCPAPLIHSVDPLTGPIDGGTRVTIRGSNLGQHVQDVLDMVRVAGVPCAVDAGEYDV SSSLVCITGASGEEVTGTVAVEVPGRGHGVSEFSFAYQDPKVHSIFPARGPRAGGTRLTLHGSKLLTGRL EDIRVVVGDQPCHLLLEQQSEQLHCETGPYPVPAELPVTVLFGATERRLQHGQFKYTSDPNVTSVGPSKS FFSGGREIWVRGQDLDVVQRPRIRVTVVPRQHGQGLAQKQHVVPEKFEEPCLVNSSHLLMCRTPALPGPP WDSGVQVEFILDNMVFDFAALSPTPFSYEADPTLRSLNPEDPSTPFRHKPGSVFSVEGENLDLAMSKEEV VAMIGDGPCVVKTLTRNHLYCEPPVEQPLPHPHALREAPDALPEFTVQMGNLRFSLGHVQYDGESPVAFP VAAQVGLGVGTSLLALGVIIIVLIYRRKSKQALRDYKKVQIQLENLESSVRDRCKKEFTDLMTEMTDLTS DLLGSGIPFLDYKVYAERFFFPGYRESPLHRDLGVPDSRRPTVEQGLGQLSNLLNSKLFLTKFIHTLESQ RTFSARDRAYVASLLTVALHGKLEYFTDILRTLLSDLVAQYVAKNPKLMLRRTETVVEKLLTNWMSICLY TFVRDSVGEPLYMLFRGIKHQVDKGPVDSVTGKAKYTLNDNRLLREDVEYRPLTLNALLAVGPGAGEAQC VPVKVLDCDTISQAKEKMLDQLYKGVPLAQRPDSCTLDVEWRSGVAGHLILSDEDVTSELQGLWRRLNTL QHYKVPDGATVALVPCLTKHILRENQDYVPGERTPMLEDVDEGGIRPWHLVKPSDEPEPPRPRRGSLRGG ERERAKAIPEIYLTRLLSMKGTLQKFVDDLFQVILSTSRPVPLAVKYFFDLLDEQAQQHGISDQDTIHIW KTNSLPLRFWINIIKNPQFVFDVQTSDNMDAVLLVIAQTFMDACTLADHKLGRDSPINKLLYARDIPRYK QMVERYYADIRQTVPASDQEMNSVLAELSRNCSADLGARVALHELYKYINKYYDQIITALEEDGTAQKMQ LGYRLQQIAAAVENKVTDL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_172775
ORF Size	6357 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_172775.1, NM_172775.2, NP_766363.2
RefSeq Size	8600 bp
RefSeq ORF	6360 bp
Locus ID	235611
UniProt ID	Q8CJH3
MW	231.8 kDa
Gene Summary	Receptor for SEMA4D (PubMed:19788569). Plays a role in GABAergic synapse development (PubMed:23699507, PubMed:29981480). Mediates SEMA4A- and SEMA4D-dependent inhibitory synapse development (PubMed:23699507, PubMed:29981480). Plays a role in RHOA activation and subsequent changes of the actin cytoskeleton (By similarity). Plays a role in axon guidance, invasive growth and cell migration (By similarity).[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.