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Tsc1 (NM_022887) Mouse Tagged Lenti ORF Clone
CAT＃: MR222584L4
- LentiORF®
Lenti ORF clone of Tsc1 (mGFP-tagged) - Mouse tuberous sclerosis 1 (Tsc1)
"NM_022887" in other vectors (4)
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CNY 10,260.00
Product images
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | hamartin; mKIAA0243 |
| Vector | pLenti-C-mGFP-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(MR222584).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_022887 |
| ORF Size | 3480 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_022887.4, NP_075025.2 |
| RefSeq Size | 7700 bp |
| RefSeq ORF | 3483 bp |
| Locus ID | 64930 |
| UniProt ID | Q9EP53 |
| Gene Summary | In complex with TSC2, inhibits the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling (By similarity). Implicated as a tumor suppressor. Involved in microtubule-mediated protein transport, but this seems to be due to unregulated mTOR signaling (PubMed:16707451). Acts as a co-chaperone for HSP90AA1 facilitating HSP90AA1 chaperoning of protein clients such as kinases, TSC2 and glucocorticoid receptor NR3C1 (PubMed:29127155). Increases ATP binding to HSP90AA1 and inhibits HSP90AA1 ATPase activity (PubMed:29127155). Competes with the activating co-chaperone AHSA1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins (By similarity). Recruits TSC2 to HSP90AA1 and stabilizes TSC2 by preventing the interaction between TSC2 and ubiquitin ligase HERC1 (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223770 | Tsc1 (untagged) - Mouse tuberous sclerosis 1 (Tsc1), (10ug) |
CNY 14,920.00 |
|
| MG222584 | Tsc1 (tGFP-tagged) - Mouse tuberous sclerosis 1 (Tsc1), (10ug) |
CNY 9,220.00 |
|
| MR222584 | Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (Tsc1) |
CNY 8,080.00 |
|
| MR222584L3 | Lenti ORF clone of Tsc1 (Myc-DDK-tagged) - Mouse tuberous sclerosis 1 (Tsc1) |
CNY 10,260.00 |
