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Rassf5 (NM_018750) Mouse Tagged ORF Clone
CAT＃: MR222330
- TrueORF®
Rassf5 (Myc-DDK-tagged) - Mouse Ras association (RalGDS/AF-6) domain family member 5 (Rassf5)
ORF Plasmid: tGFP
"NM_018750" in other vectors (4)
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CNY 3,330.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1300019G20Rik; Maxp1; Nore1; Nore1A; Nore1B; Rapl |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222330 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTCCCCGGCCATCGGGCAACGTCCCTACCCGCTGCTCCTAGATCCCGAGCCGCCGCGGTATCTGC AGAGTCTGGGTGGCACCGAGCCGCCACCTCCCGCCCGGCCGCGCCGCTGCATCCCCACGGCCCTGATCCC CGCGGCCGGGGCGTCAGAGGATCGCGGTGGCAGGAGGAGTGGCCGGAGGGACCCCGAACCCACGCCCCGA GACTGCCGACACGCTCGCCCTGTCCGGCCCGGTCTGCAGCCGAGACTGCGGCTGCGACCTGGGTCACACC GACCCCGCGACGTGAGGAGCATCTTCGAGCAGCCGCAGGATCCCCGCGTCTTGGCCGAGAGAGGCGAGGG GCACCGTTTCGTGGAACTGGCGCTGCGGGGCGGTCCGGGCTGGTGTGACCTGTGCGGACGAGAGGTGCTG CGGCAGGCGCTGCGCTGCGCTAATTGTAAATTCACCTGCCACTCGGAGTGCCGCAGCCTGATCCAGCTGG ACTGCAGACAGAAGGGGGGCCCTGCCCTGGATAGACGCTCTCCAGAAAGCACCCTCACCCCAACCTTGAA CCAGAATGTCTGTAAGGCAGTGGAGGAGACACAGCACCCGCCCACGATACAGGAGATCAAGCAGAAGATT GACAGCTATAACAGCAGGGAGAAGCACTGCCTGGGCATGAAGCTGAGTGAAGATGGCACCTACACAGGTT TCATCAAAGTGCATTTGAAGCTCCGACGGCCAGTGACGGTGCCCGCTGGGATCCGGCCCCAGTCCATCTA TGATGCCATTAAGGAAGTGAACCCTGCAGCCACCACAGACAAGCGGACTTCCTTCTACCTGCCACTCGAT GCCATCAAGCAGCTACATATCAGCAGCACCACCACGGTTAGTGAGGTCATCCAGGGGCTGCTCAAGAAGT TCATGGTTGTGGACAACCCACAGAAGTTTGCACTTTTTAAGCGGATACACAAAGATGGACAAGTGCTCTT CCAGAAACTCTCCATTGCTGACTATCCTCTCTACCTTCGTCTGCTCGCTGGGCCTGACACCGATGTTCTC AGCTTTGTGCTAAAGGAGAATGAAACTGGAGAGGTGGAGTGGGATGCCTTTTCCATTCCTGAACTCCAGA ACTTTTTAACTATCCTGGAAAAAGAGGAGCAGGACAAGATCCATCAACTGCAAAAGAAGTACAACAAATT CCGTCAGAAACTGGAAGAGGCATTACGAGAGTCCCAAGGGAAGCCGGGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222330 protein sequence
Red=Cloning site Green=Tags(s) MASPAIGQRPYPLLLDPEPPRYLQSLGGTEPPPPARPRRCIPTALIPAAGASEDRGGRRSGRRDPEPTPR DCRHARPVRPGLQPRLRLRPGSHRPRDVRSIFEQPQDPRVLAERGEGHRFVELALRGGPGWCDLCGREVL RQALRCANCKFTCHSECRSLIQLDCRQKGGPALDRRSPESTLTPTLNQNVCKAVEETQHPPTIQEIKQKI DSYNSREKHCLGMKLSEDGTYTGFIKVHLKLRRPVTVPAGIRPQSIYDAIKEVNPAATTDKRTSFYLPLD AIKQLHISSTTTVSEVIQGLLKKFMVVDNPQKFALFKRIHKDGQVLFQKLSIADYPLYLRLLAGPDTDVL SFVLKENETGEVEWDAFSIPELQNFLTILEKEEQDKIHQLQKKYNKFRQKLEEALRESQGKPG TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018750 |
| ORF Size | 1242 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018750.4, NP_061220.2 |
| RefSeq Size | 3421 bp |
| RefSeq ORF | 1242 bp |
| Locus ID | 54354 |
| UniProt ID | Q5EBH1 |
| MW | 46.7 kDa |
| Gene Summary | Potential tumor suppressor. Seems to be involved in lymphocyte adhesion by linking RAP1A activation upon T-cell receptor or chemokine stimulation to integrin activation. Isoform 2 stimulates lymphocyte polarization and the patch-like distribution of ITGAL/LFA-1, resulting in an enhanced adhesion to ICAM1. Together with RAP1A may participate in regulation of microtubule growth. The association of isoform 2 with activated RAP1A is required for directional movement of endothelial cells during wound healing (By similarity). May be involved in regulation of Ras apoptotic function. The RASSF5-STK4/MST1 complex may mediate HRAS and KRAS induced apoptosis.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC209965 | Rassf5 (untagged) - Mouse Ras association (RalGDS/AF-6) domain family member 5 (Rassf5), (10ug) |
CNY 3,990.00 |
|
| MG222330 | Rassf5 (tGFP-tagged) - Mouse Ras association (RalGDS/AF-6) domain family member 5 (Rassf5), (10ug) |
CNY 3,710.00 |
|
| MR222330L3 | Lenti ORF clone of Rassf5 (Myc-DDK-tagged) - Mouse Ras association (RalGDS/AF-6) domain family member 5 (Rassf5) |
CNY 5,230.00 |
|
| MR222330L4 | Lenti ORF clone of Rassf5 (mGFP-tagged) - Mouse Ras association (RalGDS/AF-6) domain family member 5 (Rassf5) |
CNY 5,230.00 |
