Lpin1 (NM_001130412) Mouse Tagged ORF Clone
CAT#: MR222313
- TrueORF®
Lpin1 (Myc-DDK-tagged) - Mouse lipin 1 (Lpin1), transcript variant 3
ORF Plasmid: tGFP
"NM_001130412" in other vectors (3)
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CNY 8,930.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 4631420P06; fld; Kiaa0188; Lipin1; mKIAA0188 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222313 representing NM_001130412
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTACGTGGGGCAGCTGGCCGGCCAGGTGTTTGTGACGGTGAAGGAGCTCTACAAGGGACTGAACC CCGCCACGCTGTCGGGATGCATCGACATCATTGTCATCCGGCAGCCCAATGGCAGTCTGCAGTGCTCCCC TTTCCACGTCCGCTTCGGCAAGATGGGTGTCCTCCGCTCCCGAGAGAAAGTGGTGGACATAGAAATCAAT GGGGAGTCCGTGGATTTGCACATGAAGTTGGGAGACAACGGAGAAGCATTTTTTGTTCAAGAGACTGACA ACGATCAGGAAATCATCCCCATGTACCTGGCCACGTCCCCCATCCTGTCAGAAGGAGCTGCGAGAATGGA AAGCCAGCTGAAGAGGAACTCTGTAGACAGAATCAGGTGCCTGGATCCCACTACAGCTGCCCAGGGCCTG CCTCCCAGCGACACCCCATCCACTGGTTCTCTGGGGAAGAAGAGAAGGAAAAGGAGGAGGAAGGCCCAGT TGGACAATCTCAAAAGAGATGACAATGTCAACACATCTGAGGATGAGGACATGTTTCCCATAGAGATGAG CTCGGATGAGGACACAGCACCGATGGATGGAAGCAGAACTCTTCCTAATGATGTACCACCATTCCAAGAT GATATTCCTAAGGAAAACTTCCCCTCGATTTCAACGTACCCCCAGTCAGCATCGTATCCCAGTTCGGACA GAGAATGGTCCCCCAGCCCCAGTCCTTCAGGCTCCCGGCCCTCAACACCAAAAAGTGACTCTGAGCTGGT CAGTAAGTCTGCAGACAGGTTGACGCCAAAGAATAACCTGGAAATGCTCTGGCTGTGGGGTGAATTGCCA CAGGCTGCAAAGTCCTCTTCTCCACACAAGATGAAAGAGTCCAGCCCCTTAGGGAGCCGGAAGACTCCTG ATAAAATGAATTTTCAGGCCATTCACAGCGAGTCTTCAGATACTTTTAGTGACCAGTCGCCAACAATGGC CCGGGGACTGCTCATCCACCAGAGTAAGGCCCAGACGGAAATGCAGTTTGTGAACGAGGAGGATCTCGAG TCCTTGGGGGCGGCAGCCCCACCTTCACCCGTGGCCGAAGAGCTCAAGGCCCCATATCCCAACACCGCAC AGTCGTCGAGCAAGACAGATTCCCCTTCCAGGAAGAAAGATAAACGGAGCCGACACCTTGGAGCTGATGG TGTTTATCTGGACGACCTCACGGACATGGACCCTGAAGTGGCAGCCCTGTATTTCCCCAAGAATGGGGAT CCTGGTGGGCTCCCCAAACAAGCCAGTGACAACGGAGCCAGGTCAGCCAACCAGTCACCACAGTCTGTGG GAGGCTCGGGCATCGACAGTGGTGTGGAGAGCACCTCCGACAGCCTGAGGGACCTGCCATCCATCGCCAT CTCCCTCTGCGGTGGCCTCAGTGACCACAGAGAGATCACCAAAGATGCATTTTTGGAACAAGCCGTGTCA TATCAGCAATTTGCCGACAACCCTGCTATCATCGATGACCCCAACCTCGTGGTCAAGGTTGGCAATAAGT ATTACAACTGGACAACAGCAGCTCCTCTACTTCTGGCGATGCAGGCTTTCCAGAAACCTTTGCCAAAGGC CACTGTGGAATCCATCATGAGAGATAAGATGCCCAAAAAGGGAGGAAGATGGTGGTTTTCCTGGAGAGGA AGAAATGCCACAATCAAAGAGGAAAGCAAGCCTGAACAGTGCCTGACTGGGAAAGGCCACAATACCGGAG AGCAGCCTGCCCAGCTTGGCCTGGCCACCAGGATAAAGCATGAGTCATCCTCCAGTGATGAAGAGCACGC AGCCGCCAAGCCATCAGGTTCGAGCCACCTCTCTCTCTTGTCCAACGTCAGCTACAAAAAGACCCTGCGG CTCACGTCGGAGCAGCTGAAAAGCTTGAAGTTGAAGAACGGCCCCAATGATGTGGTGTTCAGTGTCACTA CCCAGTACCAGGGCACCTGTCGCTGCGAGGGCACCATCTACCTGTGGAATTGGGACGACAAAGTCATCAT CTCAGATATCGATGGGACCATCACAAGATCTGATACTCTTGGTCACATTTTGCCCACGCTGGGAAAGGAT TGGACTCACCAGGGCATTGCAAAGCTGTACCACAAAGTAAGCCAGAATGGCTACAAGTTTCTCTATTGTT CGGCACGTGCCATTGGGATGGCGGACATGACGAGGGGCTACCTGCACTGGGTCAACGAGAGGGGCACGGT GCTTCCACAGGGCCCGCTTCTGCTCAGCCCGAGCAGCCTCTTCTCCGCCTTGCACAGAGAAGTGATTGAA AAGAAGCCAGAAAAGTTCAAAGTCCAGTGTTTGACAGACATCAAGAACCTGTTTTTCCCAAACACAGAAC CCTTCTATGCTGCTTTTGGGAACCGGCCTGCTGATGTGTATTCCTACAAGCAAGTGGGAGTGTCCCTGAA TAGGATCTTCACTGTGAACCCCAAGGGTGAGCTGGTGCAGGAGCATGCCAAGACCAACATCAGCTCGTAC GTGCGGCTCTGCGAAGTGGTCGATCACGTCTTCCCATTGCTAAAGAGAAGCCATTCCTGTGACTTCCCCT GTTCAGACACTTTCAGTAACTTCACCTTTTGGAGAGAGCCACTGCCACCTTTTGAAAACCAGGACATGCA TTCAGCCTCAGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222313 representing NM_001130412
Red=Cloning site Green=Tags(s) MNYVGQLAGQVFVTVKELYKGLNPATLSGCIDIIVIRQPNGSLQCSPFHVRFGKMGVLRSREKVVDIEIN GESVDLHMKLGDNGEAFFVQETDNDQEIIPMYLATSPILSEGAARMESQLKRNSVDRIRCLDPTTAAQGL PPSDTPSTGSLGKKRRKRRRKAQLDNLKRDDNVNTSEDEDMFPIEMSSDEDTAPMDGSRTLPNDVPPFQD DIPKENFPSISTYPQSASYPSSDREWSPSPSPSGSRPSTPKSDSELVSKSADRLTPKNNLEMLWLWGELP QAAKSSSPHKMKESSPLGSRKTPDKMNFQAIHSESSDTFSDQSPTMARGLLIHQSKAQTEMQFVNEEDLE SLGAAAPPSPVAEELKAPYPNTAQSSSKTDSPSRKKDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGD PGGLPKQASDNGARSANQSPQSVGGSGIDSGVESTSDSLRDLPSIAISLCGGLSDHREITKDAFLEQAVS YQQFADNPAIIDDPNLVVKVGNKYYNWTTAAPLLLAMQAFQKPLPKATVESIMRDKMPKKGGRWWFSWRG RNATIKEESKPEQCLTGKGHNTGEQPAQLGLATRIKHESSSSDEEHAAAKPSGSSHLSLLSNVSYKKTLR LTSEQLKSLKLKNGPNDVVFSVTTQYQGTCRCEGTIYLWNWDDKVIISDIDGTITRSDTLGHILPTLGKD WTHQGIAKLYHKVSQNGYKFLYCSARAIGMADMTRGYLHWVNERGTVLPQGPLLLSPSSLFSALHREVIE KKPEKFKVQCLTDIKNLFFPNTEPFYAAFGNRPADVYSYKQVGVSLNRIFTVNPKGELVQEHAKTNISSY VRLCEVVDHVFPLLKRSHSCDFPCSDTFSNFTFWREPLPPFENQDMHSASA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001130412 |
ORF Size | 2673 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_001130412.1, NP_001123884.1 |
RefSeq Size | 5626 bp |
RefSeq ORF | 2676 bp |
Locus ID | 14245 |
UniProt ID | Q91ZP3 |
MW | 99 kDa |
Gene Summary | Plays important roles in controlling the metabolism of fatty acids at different levels. Acts as a magnesium-dependent phosphatidate phosphatase enzyme which catalyzes the conversion of phosphatidic acid to diacylglycerol during triglyceride, phosphatidylcholine and phosphatidylethanolamine biosynthesis. Acts also as nuclear transcriptional coactivator for PPARGC1A/PPARA regulatory pathway to modulate lipid metabolism gene expression. Is involved in adipocyte differentiation. Isoform 1 is recruited at the mitochondrion outer membrane and is involved in mitochondrial fission by converting phosphatidic acid to diacylglycerol.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222313 | Lpin1 (tGFP-tagged) - Mouse lipin 1 (Lpin1) transcript variant 3, (10ug) |
CNY 9,790.00 |
|
MR222313L3 | Lenti ORF clone of Lpin1 (Myc-DDK-tagged) - Mouse lipin 1 (Lpin1), transcript variant 3 |
CNY 10,830.00 |
|
MR222313L4 | Lenti ORF clone of Lpin1 (mGFP-tagged) - Mouse lipin 1 (Lpin1), transcript variant 3 |
CNY 10,830.00 |