Prr5l (NM_001083810) Mouse Tagged ORF Clone
CAT#: MR222021
- TrueORF®
Prr5l (Myc-DDK-tagged) - Mouse proline rich 5 like (Prr5l), transcript variant 1
ORF Plasmid: tGFP
"NM_001083810" in other vectors (3)
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CNY 3,656.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | 2600010E01Rik; 4833411O04Rik; Protor-2; Protor2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR222021 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGCTCCTTCCGCCGGCCTAGACCACGCTTTATGAGCTCCCCTGTGCTCAGCGAGCTGCCCCGCTTCC AGGCTGCCCGGCAGGCTCTGCAGCTAAGTTCCAACTCTGCCTGGAACAGTGTGCAGACAGCTGTGATCAA CGTTTTCAAAGGGGGCGGCTTGCAGAGCAATGAGCTGTATGCACTGAACGAAAGCATCAGGCGGCTGCTG AAGAGTGAACTTGGATCATTCATTACTGACTATTTCCAGAACCAGCTTCTTGCAAAAGGACTGTCCTTTG TGGAGGAGAAGATCAAGCTGTGTGAAGGTGACAATCGGATTGAGGTTCTGGCTGAAGTCTGGGACCACTT CTTCACCGAGACTCTCCCTACCCTGCAGGCAATATTTTATCCGGTTCAGGGACAGGAGCTGACCATCCGC CAGATCTCCCTGCTGGGCTTCCGGGACCTGGTCTTGCTGAAGGTGAAGCTGGGTGACGTGCTGCTGTTGG CACAGTCCAAGTTGCCTTCTTCTGTTATACAAATGCTGCTCATTCTGCAGAGTGTTCACGAGCCCACAGG CCCAAGTGAGGGCTACTTGCAGCTGGAGGAGCTGGTGAAGCAAGTGGTGTCTCCCTTTCTCAGCATCAGC GGGGACCGCAGCTGCTCAGGCCCCACGTACTCACTGGCCAGGAGGCATTCCAGGGTCCGGCCCAAGGTGA CTGTCCTGAACTATGCCTCTCTGATGACCACGGTTGGCCGGCCACTGAACGAGATGGTCTTGACCCCTCT AACAGAACAGGAAGGGGAGGCATACCTGGAGAAGTGTGGCAGTGTCCGGCGGCACACAGTGGCCAACGCC CACTCAGACATCCAGCTGCTGGCGATGGCCACCATGATGCACTCAGGGTTGGGGGAGGAGGCTGGCGGCG AGGACAAACACCTGCTCTTGCCACCCAGCTTCCCCCCACCACACCGCCAGTGCTCCAGTGAACCCAGCAT CCTCGACAGCCCCGATGAACTGGAACTAGAAGACGTGGCAAGTGGCAGCCAGGAGGACTCAGAGCTGAAC TGTGCTTCCCTCAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222021 protein sequence
Red=Cloning site Green=Tags(s) MGSFRRPRPRFMSSPVLSELPRFQAARQALQLSSNSAWNSVQTAVINVFKGGGLQSNELYALNESIRRLL KSELGSFITDYFQNQLLAKGLSFVEEKIKLCEGDNRIEVLAEVWDHFFTETLPTLQAIFYPVQGQELTIR QISLLGFRDLVLLKVKLGDVLLLAQSKLPSSVIQMLLILQSVHEPTGPSEGYLQLEELVKQVVSPFLSIS GDRSCSGPTYSLARRHSRVRPKVTVLNYASLMTTVGRPLNEMVLTPLTEQEGEAYLEKCGSVRRHTVANA HSDIQLLAMATMMHSGLGEEAGGEDKHLLLPPSFPPPHRQCSSEPSILDSPDELELEDVASGSQEDSELN CASLS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_001083810 |
ORF Size | 1068 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq Size | 4296 bp |
RefSeq ORF | 1113 bp |
Locus ID | 72446 |
UniProt ID | A2AVJ5 |
MW | 39.3 kDa |
Gene Summary | Associates with the mTORC2 complex that regulates cellular processes including survival and organization of the cytoskeleton (By similarity). Regulates the activity of the mTORC2 complex in a substrate-specific manner preventing for instance the specific phosphorylation of PKCs and thereby controlling cell migration (PubMed:22609986). Plays a role in the stimulation of ZFP36-mediated mRNA decay of several ZFP36-associated mRNAs, such as TNF-alpha and GM-CSF, in response to stress. Required for ZFP36 localization to cytoplasmic stress granule (SG) and P-body (PB) in response to stress.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MG222021 | Prr5l (tGFP-tagged) - Mouse proline rich 5 like (Prr5l) transcript variant 1, (10ug) |
CNY 3,710.00 |
|
MR222021L3 | Lenti ORF clone of Prr5l (Myc-DDK-tagged) - Mouse proline rich 5 like (Prr5l), transcript variant 1 |
CNY 5,230.00 |
|
MR222021L4 | Lenti ORF clone of Prr5l (mGFP-tagged) - Mouse proline rich 5 like (Prr5l), transcript variant 1 |
CNY 5,230.00 |