Heyl (NM_013905) Mouse Tagged ORF Clone
CAT#: MR221867
- TrueORF®
Heyl (Myc-DDK-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif-like (Heyl)
ORF Plasmid: tGFP
"NM_013905" in other vectors (4)
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CNY 2,400.00
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | bHLHb33; hesr3; Hey3; Hrt3 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR221867 representing NM_013905
Red=Cloning site Blue=ORF Green=Tags(s) CTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGCGGCCCAGGGCGCCCAGTGGCTCCGACGGCGAGTCTGATGGACCCATCGATGTGGGTCAAGAGA ACGATCTTAGCCAGATGGCCAGGCCGCTGACTACCCCCAGCCCTTCGCAGATGCAAGCCCGGAAGAAGCG CAGAGGGATCATAGAGAAACGGCGCAGAGACCGCATCAACAGTAGCCTTTCTGAATTGCGACGATTGGTC CCCACTGCCTTTGAGAAACAGGGCTCCTCCAAGCTGGAGAAAGCTGAGGTCTTGCAGATGACCGTGGATC ACTTGAAGATGCTCCATGCCTCTGGTGGCACAGGGTTCTTTGATGCCCGAGCCCTGGCTGTTGACTTCCG GAGTATTGGGTTTCGGGAATGCCTCACTGAGGTCATAAGATACCTGGGGGTCCTGGAAGGACCCAGCAGC CATGCCGACCCTGTCCGGATTCGCCTCCTCTCCCACCTCAAGAGCTATGCAGCAGAGATGGAGCCCTCAC CCACGACCACCAGTGCCCTGGCCTTTCCCGTGTGGCCCTGGTCCTTCCTGCATAGCTGTCCAGGCTTGCC TTCCCTGAACAGTCAGCTCGCCATCCTGGGAAGAGTGCCCGGCCCTGTCCTCCCCAGCATCTCTTCTCCC CCTTACCCTATCTCAGCCCTGAGGTCGGCACCAGTGCACAGACCTGCTGGTACCATCCCCCCAACCCGGC GGAATTTGTTGCCCAGCCGAGGGGTGACTTCCACCCAGAGAGCCCATCTTCCTGAGAGGCCAGCTGCCCC TCCACCCACAGCCCTTGATGCCAGGGCTGCCAGGAGCATAGTCCCAATCCCACCATGTTCCCCCACAACA GCCCCTGGAGCTGGGAAATCTGATGACAATGTGTCTGGTTCTATCTCCAGTCCATGTCCTTCAGGGCCAA CTGGGAGGCCTGCGGGAGCAGTGTTCTACCACTCTTGGGTCTCAGAAATCACTGAAATTGGGGCTTTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCTGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_013905 |
ORF Size | 978 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013905.3, NP_038933.2 |
RefSeq Size | 4532 bp |
RefSeq ORF | 981 bp |
Locus ID | 56198 |
UniProt ID | Q9DBX7 |
MW | 35.4 kDa |
Gene Summary | Transcriptional repressor which binds preferentially to the canonical E box sequence 5'-CACGTG-3' (By similarity). Downstream effector of Notch signaling required for cardiovascular development. Specifically required for the Notch-induced endocardial epithelial to mesenchymal transition, which is itself criticial for cardiac valve and septum development. Represses transcription by the cardiac transcriptional activators GATA4 and GATA6.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC210027 | Heyl (untagged) - Mouse hairy/enhancer-of-split related with YRPW motif-like (Heyl), (10ug) |
CNY 3,990.00 |
|
MG221867 | Heyl (tGFP-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif-like (Heyl), (10ug) |
CNY 2,850.00 |
|
MR221867L3 | Lenti ORF clone of Heyl (Myc-DDK-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif-like (Heyl) |
CNY 4,750.00 |
|
MR221867L4 | Lenti ORF clone of Heyl (mGFP-tagged) - Mouse hairy/enhancer-of-split related with YRPW motif-like (Heyl) |
CNY 4,750.00 |