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Ipo7 (NM_181517) Mouse Tagged ORF Clone
CAT＃: MR221858
- TrueORF®
Ipo7 (Myc-DDK-tagged) - Mouse importin 7 (Ipo7)
ORF Plasmid: tGFP
"NM_181517" in other vectors (4)
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CNY 10,360.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | A330055O14Rik; C330016G14; Imp7; Ranbp7 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221858 representing NM_181517
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGATCCCAACACCATCATCGAGGCCCTCCGGGGCACCATGGACCCAGCCCTGCGCGAGGCCGCTGAGC GCCAGCTCAATGAAGCACACAAGTCTCTGAATTTCGTTTCAACACTGCTCCAGATTACTATGTCAGAACA ACTGGATTTACCTGTGAGACAGGCAGGTGTTATTTATTTAAAAAATATGATAACACAATATTGGCCTGAT CGGGAAGCCACACCAGGGGACATCGCTCCATATACTATTCCAGAAGAAGACCGGCATTGTATTCGAGAAA ATATTGTAGAAGCCATTATCCACTCACCTGAGCTTATCAGGGTACAGCTTACTACGTGCATCCATCATAT CATCAAACATGACTATCCAAGCCGCTGGACTGCCATTGTGGACAAGATTGGCTTTTATCTTCAGTCTGAT AACAGTGCTTGTTGGCTGGGAATCCTTCTTTGCCTTTATCAGCTTGTGAAAAATTATGAGTACAAGAAAC CAGAAGAGCGAAGTCCGTTAGTGGCAGCCATGCAGCACTTTCTGCCAGTTCTGAAGGATCGCTTTATCCA GCTGCTTTCTGACCAATCCGACCAGTCTGTCCTCATTCAAAAGCAGATATTCAAGATCTTCTATGCTCTT GTTCAGTATACACTACCCCTGGAGCTGATAAACCAACAGAATCTAACTGAATGGGTAGAAATTTTGAAGA CAGTTGTGAATAGGGATGTACCTAATGAAACACTTCAAGTTGAAGAAGATGATAGACCTGAGTTACCCTG GTGGAAATGTAAGAAGTGGGCCTTACATATTTTAGCAAGACTTTTTGAAAGATATGGAAGCCCTGGGAAT GTTTCTAAGGAGTATAATGAGTTTGCTGAAGTCTTTCTGAAGGCCTTTGCTGTTGGTGTCCAGCAAGTTT TATTGAAAGTGTTATATCAGTACAAGGAAAAACAATATATGGCGCCTCGAGTTTTACAGCAGACATTAAA TTATATTAATCAAGGAGTTTCCCATGCACTTACGTGGAAGAACCTGAAGCCCCATATACAAGGCATTATC CAAGATGTTATTTTTCCATTGATGTGCTATACAGATGCTGATGAGGAGCTCTGGCAAGAAGATCCTTATG AATATATACGCATGAAGTTCGATGTGTTTGAAGATTTCATTTCTCCTACTACTGCTGCTCAAACACTTTT GTTTACAGCCTGTAGCAAAAGGAAAGAGGTACTGCAAAAGACTATGGGATTTTGTTACCAAATTCTTACT GAACCCAATGCTGATCCTCGGAAAAAAGATGGAGCCTTGCATATGATTGGCTCTTTAGCTGAAATACTTC TAAAGAAAAAGATCTACAAAGATCAAATGGAATACATGTTGCAAAATCATGTATTTCCTCTCTTCAGCAG TGAGCTAGGTTACATGAGAGCAAGGGCTTGCTGGGTTCTTCACTATTTTTGTGAAGTGAAGTTCAAAAGT GATCAAAATCTCCAAACAGCTTTAGAGCTGACAAGAAGATGTCTGATTGATGATCGAGAAATGCCTGTGA AAGTGGAAGCTGCCATTGCACTACAGGTGTTGATCAGCAATCAAGAAAAAGCTAAAGAGTACATCACTCC ATTTATCAGACCTGTCATGCAGGCTCTTCTTCATATTATAAGAGAAACAGAAAATGATGATCTTACAAAT GTAATTCAGAAAATGATCTGTGAATATAGTGAAGAAGTTACTCCTATTGCTGTAGAAATGACACAACATC TGGCAATGACATTTAACCAAGTAATCCAGACTGGGCCAGATGAGGAAGGAAGTGATGACAAAGCAGTTAC TGCTATGGGAATTCTGAACACAATTGATACACTTCTGAGTGTGGTTGAAGATCACAAAGAGATAACCCAG CAGCTTGAGGGAATCTGCTTACAAGTCATTGGGACCGTTCTACAACAGCATGTCTTAGAATTCTATGAGG AGATCTTTTCTTTGGCACATAGTCTGACATGTCAACAAGTGTCTCCTCAGATGTGGCAGTTACTTCCCCT TGTGTTTGAGGTATTTCAGCAAGATGGCTTTGATTACTTTACAGACATGATGCCTCTTCTACATAACTAT GTAACAGTTGATACAGACACTCTTCTCTCTGATACCAAGTATCTGGAGATGATATACAGTATGTGTAAAA AGGTTCTTACTGGAGTCGCAGGAGAAGATGCGGAATGCCATGCGGCAAAATTGTTAGAGGTCATTATTCT GCAGTGCAAAGGCCGGGGCATTGATCAGTGTATACCATTATTCGTGGAAGCAGCTTTAGAAAGACTGACC AGAGAGGTTAAAACAAGTGAACTCAGAACAATGTGCCTGCAGGTTGCAATTGCAGCATTGTATTATAATC CACACCTACTGCTTAATACTTTAGAAAATCTTCGCTTCCCAAATAATGTTGAACCTGTTACAAATCATTT TATCACACAATGGCTTAATGACGTTGACTGTTTTCTGGGGCTTCATGACAGGAAGATGTGTGTTCTTGGC CTATGCGCCCTTATTGATATGGAACAAATACCACAGGTTTTAAATCAGGTTTCAGGACAGATTTTGCCAG CTTTTATCCTTTTATTTAATGGATTGAAAAGAGCATATGCCTGCCATGCAGAACACGAGAATGACAGTGA CGATGATGAGGATGCGGAAGATGATGATGAAACTGAAGAACTGGGGAGTGATGAAGATGATATTGATGAA GATGGACAAGAATATTTGGAGATCCTGGCTAAACAAGCTGGTGAGGATGGAGATGATGAAGATTGGGAAG AAGATGATGCTGAGGAGACTGCTCTGGAAGGCTATTCCACAATCATCGATGATGAAGACAACCCTGTTGA TGAGTATCAAATCTTTAAAGCTATATTTCAAACTATTCAAAATCGCAATCCTGTATGGTATCAGGCTTTG ACTCACGGTCTTAATGAAGAGCAAAGGAAACAGTTACAGGACATAGCAACTCTAGCTGATCAAAGAAGAG CAGCCCATGAGTCCAAAATGATTGAAAAGCATGGAGGATACAAATTCAGTGCACCAGTTGTGCCAAGTTC TTTCAATTTTGGAGGCCCAGCACCGGGGATGAAT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221858 representing NM_181517
Red=Cloning site Green=Tags(s) MDPNTIIEALRGTMDPALREAAERQLNEAHKSLNFVSTLLQITMSEQLDLPVRQAGVIYLKNMITQYWPD REATPGDIAPYTIPEEDRHCIRENIVEAIIHSPELIRVQLTTCIHHIIKHDYPSRWTAIVDKIGFYLQSD NSACWLGILLCLYQLVKNYEYKKPEERSPLVAAMQHFLPVLKDRFIQLLSDQSDQSVLIQKQIFKIFYAL VQYTLPLELINQQNLTEWVEILKTVVNRDVPNETLQVEEDDRPELPWWKCKKWALHILARLFERYGSPGN VSKEYNEFAEVFLKAFAVGVQQVLLKVLYQYKEKQYMAPRVLQQTLNYINQGVSHALTWKNLKPHIQGII QDVIFPLMCYTDADEELWQEDPYEYIRMKFDVFEDFISPTTAAQTLLFTACSKRKEVLQKTMGFCYQILT EPNADPRKKDGALHMIGSLAEILLKKKIYKDQMEYMLQNHVFPLFSSELGYMRARACWVLHYFCEVKFKS DQNLQTALELTRRCLIDDREMPVKVEAAIALQVLISNQEKAKEYITPFIRPVMQALLHIIRETENDDLTN VIQKMICEYSEEVTPIAVEMTQHLAMTFNQVIQTGPDEEGSDDKAVTAMGILNTIDTLLSVVEDHKEITQ QLEGICLQVIGTVLQQHVLEFYEEIFSLAHSLTCQQVSPQMWQLLPLVFEVFQQDGFDYFTDMMPLLHNY VTVDTDTLLSDTKYLEMIYSMCKKVLTGVAGEDAECHAAKLLEVIILQCKGRGIDQCIPLFVEAALERLT REVKTSELRTMCLQVAIAALYYNPHLLLNTLENLRFPNNVEPVTNHFITQWLNDVDCFLGLHDRKMCVLG LCALIDMEQIPQVLNQVSGQILPAFILLFNGLKRAYACHAEHENDSDDDEDAEDDDETEELGSDEDDIDE DGQEYLEILAKQAGEDGDDEDWEEDDAEETALEGYSTIIDDEDNPVDEYQIFKAIFQTIQNRNPVWYQAL THGLNEEQRKQLQDIATLADQRRAAHESKMIEKHGGYKFSAPVVPSSFNFGGPAPGMN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_181517 |
| ORF Size | 3114 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_181517.5 |
| RefSeq Size | 3117 bp |
| RefSeq ORF | 3117 bp |
| Locus ID | 233726 |
| UniProt ID | Q9EPL8 |
| MW | 119.9 kDa |
| Gene Summary | Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation (By similarity). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223340 | Ipo7 (untagged) - Mouse importin 7 (Ipo7), (10ug) |
CNY 13,400.00 |
|
| MG221858 | Ipo7 (tGFP-tagged) - Mouse importin 7 (Ipo7), (10ug) |
CNY 11,400.00 |
|
| MR221858L3 | Lenti ORF clone of Ipo7 (Myc-DDK-tagged) - Mouse importin 7 (Ipo7) |
CNY 12,260.00 |
|
| MR221858L4 | Lenti ORF clone of Ipo7 (mGFP-tagged) - Mouse importin 7 (Ipo7) |
CNY 12,260.00 |
