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Ago3 (NM_153402) Mouse Tagged ORF Clone
CAT＃: MR221842
- TrueORF®
Ago3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3)
ORF Plasmid: tGFP
"NM_153402" in other vectors (4)
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CNY 6,304.00
CNY 7,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AW048688; C130014L07Rik; Eif2c3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221842 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAATCGGCTCCGCAGGACCCATTGGGGCCCAGCCCCTATTCATCGTGCCCAGAAGACCTGGGTATG GCACTATGGGCAAACCCATTAAACTGTTGGCTAACTGTTTTCAAGTTGAAATCCCAAAGATTGATGTCTA CCTCTATGAAGTTGACATCAAACCAGACAAGTGTCCTCGAAGAGTGAACAGAGAAGTAGTTGATTCAATG GTTCAGCATTTTAAAGTGACTATATTTGGAGACCGTAGACCAGTTTATGATGGAAAAAGAAGCCTTTATA CAGCCAATCCACTTCCTGTGGCAACCACTGGGGTAGATTTAGATGTTACATTACCTGGGGAAGGTGGAAA AGATCGACCTTTCAAGGTGTCAGTCAAATTTGTGTCTCGAGTCAGCTGGCACCTACTACATGAAGCACTG GCTGGAGGGACTTTGCCTGAGCCACTGGAATTAGACAAGCCTGTCAGCACTAACCCCGTCCATGCCGTTG ACGTGGTGCTCCGACATCTGCCCTCTATGAAATATACGCCTGTGGGGCGTTCCTTTTTCTCAGCTCCAGA AGGATATGACCACCCTCTGGGAGGGGGCAGGGAAGTATGGTTTGGATTCCATCAGTCTGTTCGGCCTGCC ATGTGGAAAATGATGCTAAATATTGATGTTTCTGCCACTGCCTTTTACAAAGCACAACCTGTAATTCAGT TCATGTGTGAAGTTCTTGACATTCACAACATTGATGAACAACCCAGACCCCTGACTGATTCTCATCGGGT AAAATTCACTAAGGAGATAAAAGGTCTGAAAGTTGAAGTGACTCATTGTGGAACAATGAGACGGAAATAC CGTGTTTGTAATGTAACGAGAAGGCCTGCCAGCCACCAAACCTTTCCTTTACAGCTAGAAAATGGCCAAA CTGTGGAGAGGACTGTGGCTCAGTATTTCAGAGAAAAGTACACGCTGCAGCTGAAGTACCCACACCTTCC CTGCCTGCAAGTGGGGCAGGAGCAGAAGCATACATACCTGCCACTAGAAGTCTGTAATATTGTGGCTGGG CAAAGGTGTATCAAGAAACTAACGGACAATCAGACTTCTACAATGATAAAGGCAACAGCAAGATCTGCAC CAGATAGACAAGAGGAAATTAGCAGATTGGTAAGAAGTGCAAATTATGAAACAGATCCATTTGTTCAGGA GTTTCAGTTTAAAGTGCGGGATGAAATGGCCCATGTGACAGGACGTGTACTTCCAGCACCTATGCTCCAG TACGGAGGACGGAACCGGACAGTAGCCACACCCAGCCACGGAGTATGGGACATGCGAGGAAAACAGTTCC ACACAGGAGTCGAAATCAAAATGTGGGCTATTGCTTGCTTTGCCACACAGAGGCAGTGCAGAGAAGAAAT ACTAAAAGGTTTCACAGACCAGCTTCGGAAGATTTCCAAGGATGCAGGGATGCCCATCCAGGGTCAGCCA TGCTTCTGCAAATACGCACAGGGGGCAGACAGCGTGGAGCCCATGTTCCGGCATCTCAAGAACACCTACT CAGGACTGCAGCTCATTATCGTCATCCTGCCCGGGAAGACACCAGTGTATGCGGAAGTGAAACGTGTGGG AGATACACTTTTGGGTATGGCCACACAGTGTGTACAAGTCAAGAATGTAATAAAAACATCTCCTCAAACT CTTTCAAACTTGTGCCTAAAGATCAATGTTAAACTTGGAGGAATCAATAATATTCTTGTACCTCATCAAA GACCTTCTGTGTTCCAGCAACCAGTGATCTTCCTGGGCGCAGATGTCACACACCCGCCTGCTGGTGACGG AAAAAAGCCCTCCATTGCTGCTGTTGTAGGTAGTATGGATGCCCATCCAAGCAGATACTGTGCCACAGTA AGAGTTCAGAGACCCCGACAGGAAATCATCCAGGACTTGGCCTCCATGGTCCGGGAACTGCTCATCCAAT TTTATAAATCAACCCGATTCAAACCTACTCGTATAATCTTCTATCGGGATGGTGTTTCAGAAGGACAGTT CAGACAGGTATTATATTATGAGCTACTGGCAATTCGAGAAGCCTGTATCAGTTTGGAAAAAGACTATCAA CCTGGAATAACCTACATTGTTGTTCAGAAGAGACATCATACGCGGTTGTTCTGTGCTGATAGGACAGAAA GGGTTGGAAGAAGCGGCAACATCCCAGCTGGAACCACGGTGGATACCGACATTACTCACCCATATGAATT CGACTTTTATCTCTGTAGCCATGCTGGAATACAGGGTACCAGCCGTCCTTCACACTATCATGTTTTATGG GATGACAACTTCTTTACTGCAGATGAACTTCAGCTGCTCACTTACCAGCTCTGTCACACTTACGTACGCT GTACAAGATCTGTTTCTATCCCTGCACCAGCATATTACGCTCACCTGGTGGCATTCAGAGCCAGATATCA TCTTGTGGACAAGGAACATGACAGTGCTGAAGGAAGCCATGTTTCAGGACAGAGCAATGGGCGAGATCCA CAAGCTCTTGCCAAGGCTGTACAGATTCACCAAGATACCTTACGCACAATGTACTTCGCT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221842 protein sequence
Red=Cloning site Green=Tags(s) MEIGSAGPIGAQPLFIVPRRPGYGTMGKPIKLLANCFQVEIPKIDVYLYEVDIKPDKCPRRVNREVVDSM VQHFKVTIFGDRRPVYDGKRSLYTANPLPVATTGVDLDVTLPGEGGKDRPFKVSVKFVSRVSWHLLHEAL AGGTLPEPLELDKPVSTNPVHAVDVVLRHLPSMKYTPVGRSFFSAPEGYDHPLGGGREVWFGFHQSVRPA MWKMMLNIDVSATAFYKAQPVIQFMCEVLDIHNIDEQPRPLTDSHRVKFTKEIKGLKVEVTHCGTMRRKY RVCNVTRRPASHQTFPLQLENGQTVERTVAQYFREKYTLQLKYPHLPCLQVGQEQKHTYLPLEVCNIVAG QRCIKKLTDNQTSTMIKATARSAPDRQEEISRLVRSANYETDPFVQEFQFKVRDEMAHVTGRVLPAPMLQ YGGRNRTVATPSHGVWDMRGKQFHTGVEIKMWAIACFATQRQCREEILKGFTDQLRKISKDAGMPIQGQP CFCKYAQGADSVEPMFRHLKNTYSGLQLIIVILPGKTPVYAEVKRVGDTLLGMATQCVQVKNVIKTSPQT LSNLCLKINVKLGGINNILVPHQRPSVFQQPVIFLGADVTHPPAGDGKKPSIAAVVGSMDAHPSRYCATV RVQRPRQEIIQDLASMVRELLIQFYKSTRFKPTRIIFYRDGVSEGQFRQVLYYELLAIREACISLEKDYQ PGITYIVVQKRHHTRLFCADRTERVGRSGNIPAGTTVDTDITHPYEFDFYLCSHAGIQGTSRPSHYHVLW DDNFFTADELQLLTYQLCHTYVRCTRSVSIPAPAYYAHLVAFRARYHLVDKEHDSAEGSHVSGQSNGRDP QALAKAVQIHQDTLRTMYFA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_153402 |
| ORF Size | 2583 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_153402.1, NM_153402.2, NP_700451.2 |
| RefSeq Size | 7635 bp |
| RefSeq ORF | 2583 bp |
| Locus ID | 214150 |
| UniProt ID | Q8CJF9 |
| MW | 97.3 kDa |
| Gene Summary | Required for RNA-mediated gene silencing (RNAi). Binds to short RNAs such as microRNAs (miRNAs) and represses the translation of mRNAs which are complementary to them. Proposed to be involved in stabilization of small RNA derivates (riRNA) derived from processed RNA polymerase III-transcribed Alu repeats containing a DR2 retinoic acid response element (RARE) in stem cells and in the subsequent riRNA-dependent degradation of a subset of RNA polymerase II-transcribed coding mRNAs by recruiting a mRNA decapping complex involving EDC4 (PubMed:19174539). Possesses RNA slicer activity but only on select RNAs bearing 5'- and 3'-flanking sequences to the region of guide-target complementarity (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC222183 | Ago3 (untagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3), (10ug) |
CNY 10,930.00 |
|
| MG221842 | Ago3 (tGFP-tagged) - Mouse eukaryotic translation initiation factor 2C 3 (Eif2c3), (10ug) |
CNY 7,890.00 |
|
| MR221842L3 | Lenti ORF clone of Ago3 (Myc-DDK-tagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3) |
CNY 9,030.00 |
|
| MR221842L4 | Lenti ORF clone of Ago3 (mGFP-tagged) - Mouse eukaryotic translation initiation factor 2C, 3 (Eif2c3) |
CNY 9,030.00 |
