Cyr61 (NM_010516) Mouse Tagged ORF Clone
CAT#: MR221828
- TrueORF®
Cyr61 (Myc-DDK-tagged) - Mouse cysteine rich protein 61 (Cyr61)
ORF Plasmid: tGFP
"NM_010516" in other vectors (4)
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CNY 3,656.00
Cited in 1 publication. |
CNY 300.00
Specifications
Product Data | |
Type | Mouse Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | AI325051; CCN1; Igfbp10 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>MR221828 representing NM_010516
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCTCCAGCACCTTCAGGACGCTCGCTGTCGCCGTCACCCTTCTCCACTTGACCAGACTGGCGCTCT CCACCTGCCCCGCCGCCTGCCACTGCCCTCTGGAGGCACCCAAGTGCGCCCCGGGAGTCGGGTTGGTCCG GGACGGCTGCGGCTGCTGTAAGGTCTGCGCTAAACAACTCAACGAGGACTGCAGCAAAACTCAGCCCTGC GACCACACCAAGGGGTTGGAATGCAATTTCGGCGCCAGCTCCACCGCTCTGAAAGGGATCTGCAGAGCTC AGTCAGAAGGCAGACCCTGTGAATATAACTCCAGAATCTACCAAAACGGGGAAAGCTTCCAGCCCAACTG TAAACACCAGTGCACATGTATTGATGGCGCCGTGGGCTGCATTCCTCTGTGTCCCCAAGAACTGTCTCTC CCCAATCTGGGCTGTCCCAACCCCCGGCTGGTGAAAGTCAGCGGGCAGTGCTGTGAAGAGTGGGTTTGTG ATGAAGACAGCATTAAGGACTCCCTGGACGACCAGGATGACCTCCTCGGACTCGATGCCTCGGAGGTGGA GTTAACGAGAAACAATGAGTTAATCGCAATTGGAAAAGGCAGCTCACTGAAGAGGCTTCCTGTCTTTGGC ACCGAACCGCGAGTTCTTTTCAACCCTCTGCACGCCCATGGCCAGAAATGCATCGTTCAGACCACGTCTT GGTCCCAGTGCTCCAAGAGCTGCGGAACTGGCATCTCCACACGAGTTACCAATGACAACCCAGAGTGCCG CCTGGTGAAAGAGACCCGGATCTGTGAAGTGCGTCCTTGTGGACAACCAGTGTACAGCAGCCTAAAAAAG GGCAAGAAATGCAGCAAGACCAAGAAATCCCCAGAACCAGTCAGATTTACTTATGCAGGATGCTCCAGTG TCAAGAAATACCGGCCCAAATACTGCGGCTCCTGCGTAGATGGCCGGTGCTGCACACCTCTGCAGACCAG AACTGTGAAGATGCGGTTCCGATGCGAAGATGGAGAGATGTTTTCCAAGAATGTCATGATGATCCAGTCC TGCAAATGTAACTACAACTGCCCGCATCCCAACGAGGCATCGTTCCGACTGTACAGCCTATTCAATGACA TCCACAAGTTCAGGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221828 representing NM_010516
Red=Cloning site Green=Tags(s) MSSSTFRTLAVAVTLLHLTRLALSTCPAACHCPLEAPKCAPGVGLVRDGCGCCKVCAKQLNEDCSKTQPC DHTKGLECNFGASSTALKGICRAQSEGRPCEYNSRIYQNGESFQPNCKHQCTCIDGAVGCIPLCPQELSL PNLGCPNPRLVKVSGQCCEEWVCDEDSIKDSLDDQDDLLGLDASEVELTRNNELIAIGKGSSLKRLPVFG TEPRVLFNPLHAHGQKCIVQTTSWSQCSKSCGTGISTRVTNDNPECRLVKETRICEVRPCGQPVYSSLKK GKKCSKTKKSPEPVRFTYAGCSSVKKYRPKYCGSCVDGRCCTPLQTRTVKMRFRCEDGEMFSKNVMMIQS CKCNYNCPHPNEASFRLYSLFNDIHKFRD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_010516 |
ORF Size | 1137 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_010516.2, NP_034646.1 |
RefSeq Size | 2028 bp |
RefSeq ORF | 1140 bp |
Locus ID | 16007 |
UniProt ID | P18406 |
MW | 42.2 kDa |
Gene Summary | Promotes cell proliferation, chemotaxis, angiogenesis and cell adhesion. Appears to play a role in wound healing by up-regulating, in skin fibroblasts, the expression of a number of genes involved in angiogenesis, inflammation and matrix remodeling including VEGA-A, VEGA-C, MMP1, MMP3, TIMP1, uPA, PAI-1 and integrins alpha-3 and alpha-5 (By similarity). CCN1-mediated gene regulation is dependent on heparin-binding (By similarity). Down-regulates the expression of alpha-1 and alpha-2 subunits of collagen type-1 (By similarity). Promotes cell adhesion and adhesive signaling through integrin alpha-6/beta-1, cell migration through integrin alpha-1/beta-5 and cell proliferation through integrin alpha-v/beta-3 (By similarity).[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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Plasminogen regulates mesenchymal stem cell-mediated tissue repair after ischemia through Cyr61 activation
,Duan, H;He, Z;Lin, M;Wang, Y;Yang, F;Mitteer, RA;Kim, HJ;Yeo, E;Han, H;Qin, L;Fan, Y;Gong, Y;,
JCI Insight
,PubMed ID 32759492
[CYR61]
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Other Versions
SKU | Description | Size | Price |
---|---|---|---|
MC208761 | Cyr61 (untagged) - Mouse cysteine rich protein 61 (Cyr61), (10ug) |
CNY 3,656.00 |
|
MG221828 | Cyr61 (tGFP-tagged) - Mouse cysteine rich protein 61 (Cyr61), (10ug) |
CNY 3,710.00 |
|
MR221828L3 | Lenti ORF clone of Cyr61 (Myc-DDK-tagged) - Mouse cysteine rich protein 61 (Cyr61) |
CNY 5,230.00 |
|
MR221828L4 | Lenti ORF clone of Cyr61 (mGFP-tagged) - Mouse cysteine rich protein 61 (Cyr61) |
CNY 5,230.00 |