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Mfrp (NM_147126) Mouse Tagged ORF Clone
CAT＃: MR221747
- TrueORF®
Mfrp (Myc-DDK-tagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 1
ORF Plasmid: tGFP
"NM_147126" in other vectors (4)
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CNY 4,352.00
CNY 5,130.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | rd6 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR221747 representing NM_147126
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAAGGACTATGACGACGTCATCCTCCGTCCAGAGGCGAGTGAGTTGAGCAAGACAGAGTTCTGTAATC CTGCTTTCGACCCGGAAGCAGGGCCATCCTGCCCTCCACCAGCGTTACAGAGGGATGTCGGCAGCCGGCT CCAAGCCCCCTGGCATGCCCAGCGTCTCCGGGGGCTACAGCCCGACTGCCACTTCTCCTGGTTCTGTATT CTGCTTCTCAGTGGCCTGCTGCTCCTGCTGCTGGGGCTACTGGTGGCTGTCATCCTGGCTCAGTTGCAGG CTACATCCCTCCCCAGGACTACCAAGAACCCACTGCTCACCCGAGGCCTCACCCCCATGGGTGTCATTCC CAGCACCACCCCTAACACCACCACCACCACCACCACTACCACCCCAGCAAGGACAGGGCAGCAGGAGGCA GCCATGAGCCCTACACACCAGACCACCTGTGGAGGCCTCCTTCCTGGTCCCAGTGGTTTCTTCAGCAGCC CTAACTACCCAGACCTCTACCCACCCCTCAGCCACTGTGTCTGGCATATCCAGGTAGCCGCGGGCCAGAC AATACAGCTCAAGATTCAAGCCCTCAGCATAGAGAGTATGCTCACCTGTCTTTTTGATCGCTTGGAAATT ATCTCAGAGCCTACAGGCCCTCTCCTCAGGGTGTGTGGTAAAACACCTCCTGCCACATTAAACACCAATA CCAGCCACCTCCGTGTGTCCTTCGTCTCTGATAACGATGTGGAAGGGTCTGGTTTCCAGGCCTGGTACCA AGCAGTGGCCCCTGGACATTGGAGCTGTGCCCATAATGAGTTCCACTGTGACCTTCTCCTCTGCCTGAAG CGTGACTCTGTATGTGACGGTATTACCGAGTGTGCCGATGGCAGTGACGAGGCCAACTGCAGTGCCAAGA CGTTGGGGTGTGGAGGGAACCTGACTGGGCTCTATGGCGTATTCTCTACCCCAAACTATCCACAGCACTA CCCTCACCAACAGCTTTGCACCTGGTACATCGAAGTGCCTGTGGGATACGGGATAAGACTGGAGTTCCAC AACTTCAGTCTGGAAGCACAGGCTGAGTGCAAGTTTGACTACGTGGAAGTGTACGAGGCCAGCAACCTGG GAACCTTCAGCTTCCTGGGCAGGTTCTGTGGAGCAGAGCCACCACTCAACGTTGTCTCCTCGATGCACCA GCTGGCTGTAATCTTCAAGACGGATCTTGGTATCAGCAGCGGGGGCTTTCTAGCCACCTACCAGGCCATC AATACTACAGAGAGTGGGTGTCCCTGGGCAGAGTTCTGCCAGAGCGGAGGATATAGGGATCTGCAATGGA TGTGTGACTTATGGAAAGACTGTGCAAATGACAGCAACGACAACTGCAGCAGCCACTTGTCCCCACAACC AGACCTGACCTGTGAACCTGTCCAGGTGGAGATGTGCCTTGGACTAAGCTACAATACCACGGCCTTTCCT AACATCTGGGTGGGCCTGGCCACGCAGACAGAGGTGACAGACATCCTCCGAGGCTACAAGAGTCTGACAA GTCTACCCTGCTACCAGACTTTCCAGAGGTTCCTCTGTGGACTGCTTGTGCCTCGATGCACCTCACTGGG CACTATCCTACCCCCTTGTCGTTCCGTCTGCCAGGCGGCGGAGCAGCAGTGCCAGTCTAGCCTGGCATTA TTGGGCACCCCCTGGCCTTTCAACTGCAACAGGCTGCCCGTGGCAGCTAGCCTGGAAGCTTGCTCCCAGC CC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR221747 representing NM_147126
Red=Cloning site Green=Tags(s) MKDYDDVILRPEASELSKTEFCNPAFDPEAGPSCPPPALQRDVGSRLQAPWHAQRLRGLQPDCHFSWFCI LLLSGLLLLLLGLLVAVILAQLQATSLPRTTKNPLLTRGLTPMGVIPSTTPNTTTTTTTTTPARTGQQEA AMSPTHQTTCGGLLPGPSGFFSSPNYPDLYPPLSHCVWHIQVAAGQTIQLKIQALSIESMLTCLFDRLEI ISEPTGPLLRVCGKTPPATLNTNTSHLRVSFVSDNDVEGSGFQAWYQAVAPGHWSCAHNEFHCDLLLCLK RDSVCDGITECADGSDEANCSAKTLGCGGNLTGLYGVFSTPNYPQHYPHQQLCTWYIEVPVGYGIRLEFH NFSLEAQAECKFDYVEVYEASNLGTFSFLGRFCGAEPPLNVVSSMHQLAVIFKTDLGISSGGFLATYQAI NTTESGCPWAEFCQSGGYRDLQWMCDLWKDCANDSNDNCSSHLSPQPDLTCEPVQVEMCLGLSYNTTAFP NIWVGLATQTEVTDILRGYKSLTSLPCYQTFQRFLCGLLVPRCTSLGTILPPCRSVCQAAEQQCQSSLAL LGTPWPFNCNRLPVAASLEACSQP TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_147126 |
| ORF Size | 1752 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Note | Plasmids are not sterile. For experiments where strict sterility is required, filtration with 0.22um filter is required. |
| Reference Data | |
| RefSeq | NM_147126.3, NP_667337.1 |
| RefSeq Size | 4292 bp |
| RefSeq ORF | 1755 bp |
| Locus ID | 259172 |
| UniProt ID | Q8K480 |
| MW | 63.7 kDa |
| Gene Summary | The protein encoded by this gene contains a region with similarity to the cysteine-rich domain (CRD) of frizzled, a gene originally found in Drosophila that controls tissue polarity. This protein functions in eye development, where it is necessary for the maintenance of photoreceptor outer segments. Mutations in this gene cause retinal degeneration 6 in mice, which gives rise to a mouse model for human retinitis punctata albescens. Bicistronic transcripts composed of the coding sequences for this gene (Mfrp) and the C1q and tumor necrosis factor related protein 5 gene (C1qtnf5) have been identified, and the resulting products can interact with each other. Co-transcription of C1qtnf5 and Mfrp has been observed in both human and mouse. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jun 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219353 | Mfrp (untagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 1, (10ug) |
CNY 7,410.00 |
|
| MG221747 | Mfrp (tGFP-tagged) - Mouse membrane-type frizzled-related protein (Mfrp) transcript variant 1, (10ug) |
CNY 5,610.00 |
|
| MR221747L3 | Lenti ORF clone of Mfrp (Myc-DDK-tagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 1 |
CNY 7,030.00 |
|
| MR221747L4 | Lenti ORF clone of Mfrp (mGFP-tagged) - Mouse membrane-type frizzled-related protein (Mfrp), transcript variant 1 |
CNY 7,030.00 |
