Greb1l (NM_001083628) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mKIAA4095
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221437 representing NM_001083628 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGAACTCATATGCTGGGCAACTGAAATCTGCTAGATTTGAGGAAGCTCTACACAACTCCATAGAAG CCTCGCTCAGATGTAGTACTGCAGTGCCACGGCCAATCTTCTCCCAGCTCTACTTGGACCCAGACCAGCA TCCTTTCTCCACTGCAGATGTCAAACCAAAGGTGGAAGATCTAGATAAAGACCTAGTACATCCCTATACT CAAAATGGCAGCGTGGATTTTTCTCACAATGTAGCAATGAATGAAATGGAAGATGATGAAGATGAAGAAG AAATGTCTGACTCAAACAGCCCACCAATTCCCTACTCACAAAAACCTGCCCCAGAAGGATCTTGCACGAC AGATGGGTTTTGTCAAGCTGGAAAGGATCTGCGTTTGGTGTCACTGTGCATGGAACAAATTGACATCCCA GCCGGGTTCCTGCTGGTGGGGGCCAAGTCTCCTAACCTCCCTGAACACATCCTAGTTTGTGCTGTGGACA AGCGATTTCTACCAGATGACCATGGGAAAAATGCACTTTTAGGGTTTTCTGGAAATTGTATCGGTTGTGG AGAAAGAGGATTTCGATATTTCACAGAATTTTCCAACCACATTAACTTGAAGCTCACCACCCAGCCGAAG AAGCAGAAGCACTTAAAGTACTACCTAGTCAGGACCTCCCAGGGCGTGTTATCAAAGGGACCTCTCATCT GCTGGAAAGAATGTAGAAGCCGACAATCCTCTGCTCTTTGCCACTCCACTAAGCCTATTTCTTCAGTGTC ATCAGCTGTGGCACCAGAAAATGGAACAGCTAATGGATACAAAGCAGGCTTCACGGTGACAGAGGCAGCA AATGGGACCAGCGGCCATGGAGGCAAGAGCAGCTCATGCAGTTCCACTCCATCTCGCCCCGGGAACTACT CTTTGTCACCAAGACCTACCTTCACATCCGTAGACCAAGCTAACATGTTCATTTCTGGCCCGCCAAAGAA ACGGCACCGGGGATGGTATCCTGGGTCACCTGTCTCCCAGTCGGCTCTAGTTGTGCCAGCTCCCACAGTT CGTCCTCTTTCAAGAACTGAGCCCCTTTTATCTACCCCAGTTCCCCAGACCCCATTAACTGGAATTCTCC AGCCTCGGCCTGTCCTTGCAGGGGAAACTGTGATTGTTCCTGAAAACCTGCTGAGTAACTCAGGAGTTAG ACCAGTGATCCTGATAGGCTATGGCACTTTACCCTATTTCTATGGAAATGTTGGTGACATTGTTGTGAGT CCTCTGCTGGTGAATTGCTACAAGATTCCACAATTGGAAAACAAAGATCTGGAACAATTAGGGTTAACTA GCACCCATCTCCTGAGTGTGGAGAACATGATCCTTCTGACTATACAGTACCTTGTACGATTAGGTCCTGA CCAGATTCCTCTCCGGGAAGAGTTTGAGCAGATCATGCTGACTGCTATGCAGGAGTTTTCTGTGAGAGAG CGAGCCCTGCCTTTGGGCGCTCCGTGTGCACCCATGTCGCCGGCACAGCTCCCCTGGCTGGCTAGGCTGG CGGCCAGTGTGTCTCAGGACCTGGTTCATGTGATTGTGACCCAGAACTCTCTAGCAGAAGGCATTTCAGA GACGCTGAGGCTTCTCAGTGAAATGAAACACTACCAGAGGCTGCCAGATTACGTCGTGGCAATTTGTGCC TCAAAAATCAGAGGAAATGAATTTTGTGTGGTAGTATTAGGTCAGCATCAGTCCCGGGCCCTGGCAGAGA GCATGCTTACCACAAGTGAGTTTTTGAAAGAAATTAGCTATGAGCTCATCACAGGAAAGGTCAGCTTCCT GGCATCACATTTCAAAACAACATCATTAGGGGATGACCTGGACAAGCTGCTTGAAAAAATGCAACAAAGA AGAGGAGACAGTGTAGTCACTCCTTTCAATGGTGACCTTGATGAGTGTGTGTCCCCTCAGGAGGCTGCTG CTATGATTCCCACACAAAACCTGGATGTAGATAACGAAACCTTCCAAATTTATCAACCACAGCTCACAGT TGCCAGAAGACTCTTATCCCAGGTGTGTGCAATCGCAGACAGCGGCAGCCAGAGCCTGGACCTCGGCCAC TTTAGCAAAGTGGACTTCATCATCATCGTCCCAAGGTCAGAGGTTCTGGTCCAGCAAACTCTCCAGCGTG TTCGACAGTCAGGTGTCCTTGTAGACCTGGGTCTGGAGGAAAGTGGGCTGGCTCATCAGAGAGCAGAGAG ATACGTTGTTCGACTGGACAATGAGATCCAAAGCAAGTTTGAAGTTTTCATGAGGAGGGTGAAGCAGAAC CCATACACACTGTTTGTGCTGGTTCATGACAACTCCCATGTGGAGCTAACGAGTGTGATTTCAGGCTCTC TGTCACATGGTGAGCCAACCCACGGATTGGCTGATCGAGTTATTAATTGCAGAGAAGTTCTCGAAGCTTT CAACCTCCTGGTGCTCCAGGTGAGCTCCTTCCCATACACACTGCAGACCCAACAGTCTCGAATCAGCTCC AGCAACGAGGTCCACTGGATACAACTGGATACAATGGAGGACGCAGGCTGCGAGAAGCTGTACTTTGGCT TGGATGAGTACAGCAAGTCTCTGCAGTGGGGGATCACCAGCCCACTCCTGAGATGCGATGAGACCTTTGA GAAGATGGTGAGCACCCTCTTGGAGAGGTACCCCCGGCTGCATAGCATGGTCGTGCGCTGCTATCTACTC ATCCAGCAGTACTCGGAGGCCTTGATGGCTCTCACCACTATGGCGTCCCTCCGAGACCACAGCACACCAG AAACGCTCAGCATTATGGACGACCTTATCACCTCCCCAGGAAAAAACAAAAGCGGGAAGGGACACATGCT CGTCATCAGAGTGCCTTCTGTGCAGCTGGCGATGCTGGCCAAGGAGCGGCTGCAGGAAGTGCGAGACAAG CTGGGTCTGCAGTATCGCTTTGAGATCATCCTGGGGAGCCCCGCCTCCGAACTCACTGTTGAAACTCACT TTGTAACCCGACTAAAGACCTGGAGAGGAAATGACCAGGACGAGTGGATCCCTCGGACATATCAAGACTT GGAAGGTCTGCCCTGCATAGTGATATTGACTGGCAAGGACCCTCTTGGAGAAACTTTCCCCAGGTCCTTA AAGTACTGTGACCTGCGATTAATCGACTCAAGCTATTTAACTCGAACGGCCTTGGAGCAGGAGGTGGGCC TGGCATGCTGCTATGTCTCCAAAGAGGTCATCCGGGGGCCTGCTGCTGCCCTGGACCTCAGTGCAAAGGA GGCTGAGAGGGTCCCCGCCAGTGAGAATGATTCTGAGGAGCTGCTCATCGATTTGGAAAGGCCACAGAGC AACAGTAGCGCTGTCACAGGAACCTCAGGTTCCATCATGGAGAATGGGGTGAGTTCTTCTAGCACGGCTG GCAAACCCCAGCAGCAGCTTCTGACCCCCACCAGCAGCATTCGGCTGGATGAAGGGGTCTCTGCCAGCAC CGCTGTGGTCGGGGAGATCCTGAAGCAGGAGTGTGACTCCTTGGACCCTCCGATGGCCAGTAGCACCACC TCCAAGCCTTCTTCATCCTCATCCTCGAGTGCCCAGGCTCTTGCGTGGTCAAGGCAGCCCCGGGGCCTGC ACACAGCCCTGCCCCCCGTGATCATCTTATCCAAAGCCGCATACAGCCTGCTGGGATCCCAGAAGGGCGG CAGGCTGCCATCCTCCTCGTCCTTGCTGCCCCATGCTGATGTGGCCTGGGTGAGCTCCCTGCGACCAGGC CTGCACAAAGACATGAGCAGCGAGGAGCAGTCCCTTTATTACCGCCAGTGGACCTCAGCCCGGCAGCACC ACGCTGACTACAGCAACCAGCCAGACCCCATCTCAGGCGCCCGAACCTTGCACCCGAGGCGGCTGCTGCT CACTGGACCCCCCCAGGTGGGGAAGACCGGCTCCTATCTACAGTTCCTTAGGATCCTGTTCCGAATGCTC ATCCGGCTGCTGGAGGTAGACGTGTACAATGAGGAAGAGATAAACACAGATCACAGCGATGACAGCGAAC TGAGCCAGTCAGAGGGAGAGCCCTGGCCTGACATCGAGACCTTCAGCAAAATGCCTTTCGACGTCAGTGT ACACGACCCCAAGTACCGTCTGATGAGCCTGGTCTACAGTGAGAAGCTGGCTGGCATCAAGCAAGAAGTA ATAAAGGAATACAAAGTGGAGGAGCCCCGGCAACGAGAAACCATGTCTATGATGCTAACCCAGTATGCAG CCTATAATACCTTTCACCACTGTGAACAGTGCCAGCAGTACATGGCCTTCACCCCTGCCTCCCAGATGTC CGATTCCACCCTTCACGCATTCACGTTCTCTTCTTCTATGCTGGGAGAAGAAGTCCAGCTCTATTTCATC ATCCCCAAGTCCAAAGAGAGTCATTTTGTCTTCAGCAAGCAGGGCAGACATCTGGAAAGCATGCGGCTGC CCCTGGTTTCTGACAAGAATCTAAATGCAGTCAAGAGTCCTATTTTCACTCCTTCCAGTGGTCGCCATGA ACATGGGCTCCTAAACCTTTTCCACGCCATGGAGGGCATCAGCCACCTTCACCTCCTGGTAGTCAAAGAA TATGAGATGCCACTATACCGAAAATACTGGCCCAACCACATCATGCTGGTGCTTCCTGGCATGTTTAATA ATGCAGGCGTGGGTGCTGCTAGGTTCCTCATTAAGGAGCTGTCATATCACAACCTAGAGCTGGAAAGGAA TCGCCTGGAGGAACTGGGAGTCAAGCGGCAGTGTGTCTGGCCTTTCATCGTTGTGATGGATGACTCCTGT GTCCTGTGGAACATTCACAGTGTTCAGGAGCAAACCAGCCAGCCTACAGAAGCAGGAATTTCCAGTAAGA ATGTGTCCCTGAAGAGCGTCTTGCAGCATATTGAAGCCACACCAAAAATTATCCACTACGCAATCCTGGG CATACAGAAATGGAACAGCAAGCTGACTTCACAGAGCCTAAAGGCCCCGTTCTCAAGATGTCATGTGCAT GATTTCATTCTCCTCAATATAGACCTAACTCAGAATGTACAGTATGACTTCAACAGGTATTTCTGTGAAG ACGTTGACTTTAACCTGAGAACAAACAGCAGTGGCCTCCTCATCTGCCGCTTTAATAACTTCAGTCTCAT GAAGAAGCACGTTCAGGTTGGAGGGCAAAGGGACTTCATCATTAAACCAAAGCTCATGGTTTCAGAGAAC GTAGTCCCCATCCTGCCCCTTCAGTACGTCTGCGCCCCTGACAGTGAACACACACTCCTGGCAGCCCCTG CACAGTTCCTCCTGGAGAAGTTCCTTCAACATGCCTCATATAAACTCTTCCCTAAAGCCATCCATAACTT CAAGAGTCCTGTGCTGGCCATTGACTGCTACCTTAACATTGGACAGGAGGTAGCCATATGCTATGTCAGC TCCAGACCACACTCCAGCAACGTCAATTGTGAAGGGGTGTTCTTCAGTGGACTCCTCCTGTACCTCTGCG ACTCTTTTGTAGGTGCTGATCTTCTCAAGAAATTCAAGTTTCTAAAAGGTGCTACCCTGTGTGTCATCTG CCAAGACAGAAGCTCCTTACGTCAAACAATTGTCCGCTTAGAGTTAGAGGATGAGTGGCAGTTCCGCCTC CGGGATGAGTTTCAAACTGCTAACAGCAGTGATGACAAGCCTCTCTACTTTCTTACTGGACGCCATGTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221437 representing NM_001083628 Red=Cloning site Green=Tags(s) MGNSYAGQLKSARFEEALHNSIEASLRCSTAVPRPIFSQLYLDPDQHPFSTADVKPKVEDLDKDLVHPYT QNGSVDFSHNVAMNEMEDDEDEEEMSDSNSPPIPYSQKPAPEGSCTTDGFCQAGKDLRLVSLCMEQIDIP AGFLLVGAKSPNLPEHILVCAVDKRFLPDDHGKNALLGFSGNCIGCGERGFRYFTEFSNHINLKLTTQPK KQKHLKYYLVRTSQGVLSKGPLICWKECRSRQSSALCHSTKPISSVSSAVAPENGTANGYKAGFTVTEAA NGTSGHGGKSSSCSSTPSRPGNYSLSPRPTFTSVDQANMFISGPPKKRHRGWYPGSPVSQSALVVPAPTV RPLSRTEPLLSTPVPQTPLTGILQPRPVLAGETVIVPENLLSNSGVRPVILIGYGTLPYFYGNVGDIVVS PLLVNCYKIPQLENKDLEQLGLTSTHLLSVENMILLTIQYLVRLGPDQIPLREEFEQIMLTAMQEFSVRE RALPLGAPCAPMSPAQLPWLARLAASVSQDLVHVIVTQNSLAEGISETLRLLSEMKHYQRLPDYVVAICA SKIRGNEFCVVVLGQHQSRALAESMLTTSEFLKEISYELITGKVSFLASHFKTTSLGDDLDKLLEKMQQR RGDSVVTPFNGDLDECVSPQEAAAMIPTQNLDVDNETFQIYQPQLTVARRLLSQVCAIADSGSQSLDLGH FSKVDFIIIVPRSEVLVQQTLQRVRQSGVLVDLGLEESGLAHQRAERYVVRLDNEIQSKFEVFMRRVKQN PYTLFVLVHDNSHVELTSVISGSLSHGEPTHGLADRVINCREVLEAFNLLVLQVSSFPYTLQTQQSRISS SNEVHWIQLDTMEDAGCEKLYFGLDEYSKSLQWGITSPLLRCDETFEKMVSTLLERYPRLHSMVVRCYLL IQQYSEALMALTTMASLRDHSTPETLSIMDDLITSPGKNKSGKGHMLVIRVPSVQLAMLAKERLQEVRDK LGLQYRFEIILGSPASELTVETHFVTRLKTWRGNDQDEWIPRTYQDLEGLPCIVILTGKDPLGETFPRSL KYCDLRLIDSSYLTRTALEQEVGLACCYVSKEVIRGPAAALDLSAKEAERVPASENDSEELLIDLERPQS NSSAVTGTSGSIMENGVSSSSTAGKPQQQLLTPTSSIRLDEGVSASTAVVGEILKQECDSLDPPMASSTT SKPSSSSSSSAQALAWSRQPRGLHTALPPVIILSKAAYSLLGSQKGGRLPSSSSLLPHADVAWVSSLRPG LHKDMSSEEQSLYYRQWTSARQHHADYSNQPDPISGARTLHPRRLLLTGPPQVGKTGSYLQFLRILFRML IRLLEVDVYNEEEINTDHSDDSELSQSEGEPWPDIETFSKMPFDVSVHDPKYRLMSLVYSEKLAGIKQEV IKEYKVEEPRQRETMSMMLTQYAAYNTFHHCEQCQQYMAFTPASQMSDSTLHAFTFSSSMLGEEVQLYFI IPKSKESHFVFSKQGRHLESMRLPLVSDKNLNAVKSPIFTPSSGRHEHGLLNLFHAMEGISHLHLLVVKE YEMPLYRKYWPNHIMLVLPGMFNNAGVGAARFLIKELSYHNLELERNRLEELGVKRQCVWPFIVVMDDSC VLWNIHSVQEQTSQPTEAGISSKNVSLKSVLQHIEATPKIIHYAILGIQKWNSKLTSQSLKAPFSRCHVH DFILLNIDLTQNVQYDFNRYFCEDVDFNLRTNSSGLLICRFNNFSLMKKHVQVGGQRDFIIKPKLMVSEN VVPILPLQYVCAPDSEHTLLAAPAQFLLEKFLQHASYKLFPKAIHNFKSPVLAIDCYLNIGQEVAICYVS SRPHSSNVNCEGVFFSGLLLYLCDSFVGADLLKKFKFLKGATLCVICQDRSSLRQTIVRLELEDEWQFRL RDEFQTANSSDDKPLYFLTGRHV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms	CHROMATOGRAMS Sequencher program is needed, download here.
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001083628
ORF Size	5739 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001083628.1, NP_001077097.1
RefSeq Size	8421 bp
RefSeq ORF	5742 bp
Locus ID	381157
UniProt ID	B9EJV3
MW	213.6 kDa
Gene Summary	Plays a major role in early metanephros and genital development.[UniProtKB/Swiss-Prot Function]

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